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Made available in DSpace on 2021-07-14T10:40:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-23. Added 1 bitstream(s) on 2021-07-14T11:35:34Z : No. of bitstreams: 1 S0006-87052018000200348.pdf: 358042 bytes, checksum: 4a1a824c4d9939542af71b45e86bcd2f (MD5) São Paulo Research Foundation Safflower (Carthamus tinctorius L.) is a commercial seed crop grown for its good yield of high-quality oil. It is tolerant to water stress but may be sensitive to soil compaction. The aim of this study was to assess safflower growth under different degrees of soil compaction at depths of 0.15 m to 0.20 m. The experiment was carried out in PVC pots constructed from three rings. Five levels of penetration resistance (0.20, 0.33, 0.50, 0.93, and 1.77 MPa) were applied in the intermediate ring, and two safflower genotypes, IMA-4904 and IMA-2106, were examined. There was no difference between safflower genotypes with respect to their resistance to soil compaction, which reduced root length density in the compacted layer and changed the root distribution in the soil profile, but did not prevent the roots from crossing the compacted layer and developing in depth. Increased soil bulk density in the compacted layer increased root diameter of the IMA-2106 genotype. Penetration resistance levels over 0.20 MPa (density of 1.2 mg.dm–3) limited safflower root development. The maximum safflower growth occurred when the soil penetration resistance was 0.86 MPa. In this study, the Q1/2 index was higher than 1.77 and 1.55 for the IMA-2106 and IMA-4904 genotypes, respectively. Hence, safflower has proven to be tolerant to soil compaction, and stands out as a species with potential to decrease soil bulk density. Universidade Estadual Paulista “Julio de Mesquita Filho”, Faculdade de Ciências Agronômicas Universidade Estadual Paulista “Julio de Mesquita Filho”, Faculdade de Ciências Agronômicas São Paulo Research Foundation: 2014/10656–3; 2016/14323–4

Made available in DSpace on 2019-10-04T21:46:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01. Added 1 bitstream(s) on 2021-07-15T15:23:24Z : No. of bitstreams: 1 S1983-40632018000200163.pdf: 287442 bytes, checksum: 5b7fdce67a2feaf5b33354449d340292 (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Guar (Cyamopsis tetragonoloba L.) is commonly grown in arid lands, because of its high drought-tolerance. However, soil compaction may be a limiting factor to its growth. This study aimed to evaluate the guar growth, according to the soil penetration resistance (0.20 MPa, 0.33 MPa, 0.50 MPa, 0.93 MPa and 1.77 MPa, in a layer with depth between 0.15 m and 0.20 m), in a Rhodic Acrudox soil. The shoot and root dry mass, root length by the Q(1/2 )index (mechanical soil penetration resistance in which the root growth is reduced by 50 %) and root diameter were evaluated. The impairment of the guar shoot growth begins when the penetration resistance is greater than around 1 MPa. The soil compaction alters the distribution of guar roots in the soil profile, concentrating them in the 0.15 m layer, but it does not prevent roots from penetrating this layer and developing in depth. The root diameter increases in the compacted layer. A soil penetration resistance of up to 1.77 MPa does not influence the root length density below the compacted layer, as well as the total root length density of guar. Although the guar Q(1/2) index is greater than 1.58, the shoot and root dry mass are impaired. Univ Estadual Oeste Parana, Cascavel, PR, Brazil Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA Univ Estadual Paulista, Fac Ciencias Agron, Dept Agr, Botucatu, SP, Brazil Univ Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil Univ Estadual Paulista, Fac Ciencias Agron, Dept Agr, Botucatu, SP, Brazil Univ Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil FAPESP: 2014/10656-3 FAPESP: 2016/14323-4

Objective The objective was to characterize the fermentative and microbiological profile of Tifton 85 bermudagrass haylage with different layers of polyethylene film and storage time. METHODS The experimental design consisted of a randomized block design with four and six wrapping layers (100 and 150 microns in total. respectively) allocated in the main plots, through repeated measures analysis (30, 60, and 90 days of storage) with four replicates. RESULTS The storage time and number of wrapping layers did not show changes in the population of Clostridium and lactic acid bacteria. A decrease was observed in the enterobacteria population with an increase in the storage period in the two wrapping layers studied. Upon opening of the haylage at 30 days, the population of Bacillus was lower in haylages made with six layers of wrapping (3.63 log colony forming units/g). No growth of Listeria sp. or Salmonella sp. was observed during the experimental period. The fungal genera with a greater occurrence were Penicillium sp. and Fusarium sp. The following mycotoxins were not detected: ochratoxin A, fumonisins, and zearalenone. Relative to the organic butyric, propionic, and acetic acids, the haylages presented a low concentration of lactic acid; this may have prevented a drop in the pH, which was high when the silos were opened (5.4). The levels of ammoniacal nitrogen and soluble carbohydrates presented no variation among the number of wrapping layers, with an overall average of 35.55 and 38.04 g/kg. CONCLUSION Tifton 85 bermudagrass haylage wrapped with four and six layers presented adequate fermentation and microbiological characteristics in the evaluated periods.

The objective of this study was to evaluate the effects of biodigester treatment on the microbiological characteristics of swine wastewater (SW) at the production and storage sites, and to characterize and compare the microbiological composition of the soil, organic matter, and plants in the area of hay and haylage production. The area has been planted with Tifton 85 grass for eight years; and is exclusively intended for hay and haylage production, SW was used as the only fertilizer source. The experimental design was completely randomized with subdivided plotsin timeand five replications; the plots were the main areas of the SW in natura (affluent), the biodigester outlet (effluent), the storage pond, and the area of Tifton 85 production, which included the soil, plant residue on the soil surface (organic matter), and the aerial parts of the grasseswith subplots in the rainy and dry seasons. The microbial count in the SW was reduced during the treatment process, with higher counts in the affluent, and lower countsin the effluent and storage pond. The SW treatment was efficient in reducing the microbial population. The populations of Bacillus and Clostridiumwere influenced by the season of the year, with larger populations during the rainy season (summer) than during the dry season (winter). The mold genera identified in the SW area were Penicillium, Rhizopus, Fusarium, Helminthosporium, and Phoma. The genera Penicillium, Rhizopus, Fusarium, Cladosporium, Helminthosporium, Bipolaris, Phoma, Aspergillus, and Trichoderma were found in the area of Tifton 85 production.

This study aimed to evaluate the fractionation of carbohydrates and protein of grass hay Tifton 85 bermudagrass under two cutting heights in relation to ground level (0.04 and 0.08 m) during 120 days of storage. Samples were collected in the baling and hay stored in shed at 30, 60, 90 and 120 days of storage, after, it was subjected to laboratory procedures, in which the levels of soluble carbohydrates were determined, carbohydrate fractionation and protein fractionation. The results were studied in a randomized complete block design with split plot with two treatments allocated the plots: cutting height of 0.04 to 0.08 m from the ground and five times in the subplots: baling, 30, 60, 90 and 120 days of storage hay, with five repetitions. The soluble carbohydrate concentration hay responded in a quadratic manner to the storage time for cutting heights of 0.04 and 0.08 m above soil level; minimum values of 11.53 and 10.70 g kg-1 of DM were estimated for 93.44 and 91.16 storage days, respectively. The carbohydrate fraction A + B1 responded negatively linear to periods of storage evaluated in both cutting heights. The contents of the carbohydrate fraction B2 and proteins A and C exhibited a positive linear response to storage time for a cutting height of 0.04 m and a quadratic effect for hay cutting height at 0.08 m above soil level, with an opposite behavior for both cutting heights for the B1 protein levels and C carbohydrate fraction. The B2 fraction showed a negative linear response as a function of storage time, with a reduction in the fraction of 0.09 and 0.11 percentage units for each day of storage, for hay cutting height of 0.04 and 0.08 m, respectively. The B3 fraction responded quadratically to the storage time, the two studied cutting heights. The storage of Tifton 85 hay cut at 0.04 m above soil level for 120 days leads to a decrease in fractions of rapid ruminal degradation and increase of the indigestible fraction in carbohydrate and protein fractions. Based on the results obtained it is recommended the cutting of the Tifton 85 bermuda grass forage to produce hay at 0.08 m and its storage up to 30 days provided that under ideal conditions. Este trabalho objetivou avaliar o fracionamento de carboidratos e proteína do feno de capim Tifton 85 sob duas alturas de corte em relação ao nível do solo (0.04 e 0.08 m), durante 120 dias de armazenamento. Foram coletadas amostras no enfardamento e no feno armazenado em galpão aos 30, 60, 90 e 120 dias de armazenamento. Após foram submetidas a procedimentos laboratoriais, onde foram determinados os teores de carboidratos solúveis, fracionamento de carboidrato e fracionamento de proteína. Os resultados foram estudados sob o delineamento em blocos casualizados com parcelas subdivididas no tempo com 2 tratamentos alocados nas parcelas: altura de corte de 0.04 e 0.08 m do solo e cinco tempos nas subparcelas: enfardamento, 30, 60, 90 e 120 dias de armazenamento do feno, com cinco repetições. A concentração de carboidratos solúveis respondeu de forma quadrática ao tempo de armazenamento, para altura de corte de 0.04 e 0.08 m do solo, estimando-se valores de mínima de 11.53 e 10.70 g kg-1 de MS aos 93.44 e 91.16 dias de armazenamento, respectivamente. A fração de carboidrato A + B1, respondeu de forma linear negativa aos períodos de armazenamento avaliados nas duas alturas de corte, não diferindo entre as alturas. Os teores da fração de carboidratos B2 e de proteína A e C apresentaram resposta linear positiva em função do tempo de armazenamento para altura de corte a 0.04 m e efeito quadrático para altura de corte do feno a 0.08 m do solo, sendo observado comportamento inverso em ambas as alturas de corte para os teores protéicos B1 e de carboidratos fração C. A fração proteica B2 apresentou resposta linear negativa em função do tempo de armazenamento, com redução da fração de 0,09 e 0,11 unidades percentuais a cada dia de armazenamento, para altura de corte do feno a 0.04 e 0.08 m, respectivamente. A fração B3 respondeu de forma quadrática ao tempo de armazenamento, nas duas alturas de corte estudadas. O armazenamento dofeno de capim Tifton 85 cortado a 0.04 m de altura do solo, durante 120 dias, ocasiona diminuição das frações de rápida degradação ruminal e aumento da fração indigestível, nos fracionamentos de carboidratos e de proteína. Recomenda-se o corte da forrageira Tifton 85 para produção de feno a 0.08 m e seu armazenamento até 30 dias em condições ideais.

This study aimed to evaluate the microbiological profile and aerobic stability of grass silage Tifton 85, with or without vacuum, and with or without microbial inoculants. The experimental design was completely randomized in a 2 x 2 factorial design, where the treatments included five replicates, with and without vacuum or addition of inoculants. The inoculum consisted of lactic acid bacteria (LAB), specifically Lactobacillus acidophilus, at a concentration of 3 x 109 CFU mL-1 per mL. The analyzed variables included the microbiological profile after opening the silage, as well as the aerobic stability at the time of opening on the sixth day of the silage's exposure to oxygen. It was found that no variation occurred in the population of lactic acid bacteria between the applied treatments. The Bacillus population was lower irrespective of the inoculant application, since it was applied in vacuum. When the population of Clostridium was applied, there was a reduction in the inoculant population in the vacuum system compared to that of the non-vacuum system. Without applying the inoculant, there was also a reduction in the population of Clostridium in the non-vacuum system. The yeast population showed linear growth in all of the evaluated treatments from the first to the sixth day of exposure to air, which may have contributed to the high temperatures observed during the air exposure period. There was no growth of fungi in the silage during the period of exposure to oxygen. The breaking of the aerobic stability occurred from the 3rd day after opening the silage. The pH was below the level that is considered to be good for silage preservation at the time of opening. In addition, during the exposure to oxygen, the same phenomenon occurred with the temperature, which demonstrated a quadratic behavior during the study period. Under the conditions evaluated, Tifton 85 bermudagrass silage lost its stability after the third day of exposure to air. A pesquisa objetivou avaliar o perfil microbiológico e a estabilidade aeróbia em silagens de capim Tifton 85, a vácuo e sem vácuo, adicionados ou não inoculante microbiano. O delineamento experimental foi inteiramente casualizado em esquema fatorial 2x2, sendo os tratamentos com e sem vácuo e com e sem adição de inoculante, com cinco repetições. O inoculante era composto por bactérias ácido láticas (BAL): Lactobacillus acidophilus, na concentração de 3 x 109 UFC ml-1 de células viáveis por ml do produto. As variáveis analisadas foram o perfil microbiológico após abertura da silagem e a estabilidade aeróbia da abertura ao sexto dia de exposição da silagem ao oxigênio. Verificou-se que não houve variação na população de bactérias láticas entre os tratamentos aplicados. A população de Bacillus foi inferior tanto com aplicação quanto sem aplicação de inoculante desde que aplicado o vácuo. A população de Clostridium quando aplicado o inoculante, o sistema a vácuo reduziu a população em comparação ao sistema sem vácuo. Sem a aplicação de inoculante o sistema sem vácuo reduziu a população de Clostridium. A população de leveduras apresentou tendência a crescimento linear em todos os tratamentos avaliados do primeiro ao sexto dia de exposição ao oxigênio o que pode ter contribuído para as altas temperaturas observadas no período de exposição ao ar. Não se observou crescimento de fungos nas silagens durante o período de exposição ao ar. A quebra da estabilidade aeróbia ocorreu a partir do 3º dia após abertura da silagem. O pH ficou abaixo do preconizado para uma boa conservação da silagem na abertura e durante a exposição aooxigênio, com comportamento quadrático, o mesmo ocorrendo com a temperatura no período avaliado. Silagens de capimtifton 85 nas condições avaliadas perdem a estabilidade após o terceiro dia de exposição ar.

Tropical soils are highly weathered, acidic, and low in silicon (Si) availability for plants. Si has been considered an essential nutrient for many grasses. Urochloa Convert HD364 is classified as a forage plant that accumulates Si, but the accumulation of this nutrient in the leaf can influence qualitative characteristics, fiber quality, plant architecture, and development of forage. In this study, we aimed to evaluate the production and nutritive value of Urochloa hybrid cultivar Convert HD364 (CIAT 36087) grown from soils collected in the state of Paraná, Brazil. The experiment was carried out in a greenhouse using 8-L plastic pots and three types of soil. Treatments were arranged in a randomized block design in a 3 × 5 factorial: three soils [Rhodic Acrudox (Ox1), Rhodic Hapludox (Ox2) and Arenic Hapludult (Ult)] and five silicate rates (0, 1, 2, 4 and 6 Mg ha-1 of calcium/magnesium silicate), with four replications. Application of CaSiO3 to the soil increases the concentration of Si in the leaves of Urochloa Convert HD364. The dry matter, crude protein and mineral matter, fiber quality, and digestibility of Urochloa were not influenced by the increase in Si levels in the leaves until 45 days after seeding. Forty-five days after planting, CaSiO3 did not interfere with the growth characteristics and production of Urochloa Convert HD364. There is no evidence that the increased Si levels in the leaf affected the production and nutritive value, especially fiber quality of Urochloa Convert HD364 until 45 days after sedding. Os solos tropicais são altamente intemperizados, ácidos e com baixa disponibilidade de silício (Si) para as plantas. O Si tem sido considerado um nutriente essencial para muitas gramíneas. A Urochloa Convert HD364 é classificada como planta forrageira que acumula Si, mas o acúmulo desse nutriente na folha pode influenciar as características qualitativas, qualidade da fibra, arquitetura da planta e no desenvolvimento da forragem. Neste trabalho, objetivou-se avaliar a produção e o valor nutritivo da Urochloa cv. Convert HD364 (CIAT 36087), cultivado em três solos coletados no estado do Paraná, Brasil. O experimento foi conduzido em casa de vegetação com vasos plásticos de 8 L e três tipos de solo. Os tratamentos foram arranjados em blocos casualizados em esquema fatorial 3 × 5: três solos [Latossolo Vermelho distrférrico (Ox1), Latossolo Vermelho eutroférrico (Ox2) e Argisolo Vermelho-Amarelo (Ult)] e cinco doses de silicato (0, 1, 2, 4 e 6 Mg ha- 1 de silicato de cálcio / magnésio), com quatro repetições. A aplicação de CaSiO3 no solo aumenta a concentração de Si nas folhas de Urochloa Convert HD364. A matéria seca, proteína bruta, matéria mineral, qualidade da fibra e digestibilidade da Urochloa Convert HD364 não foram influenciadas pelo aumento nos teores de Si nas folhas até 45 dias após a semeadura. Quarenta e cinco dias após o plantio, o CaSiO3 não interferiu nas características de crescimento e produção do Urochloa Convert HD364. Não há evidências de que o aumento dos níveis de Si na folha tenha afetado a produção e o valor nutritivo, especialmente a qualidade da fibra, de Urochloa Convert HD364 até 45 dias após a semeadura.

Wheat is a plant that accumulates silicon (Si). The application of silicon to the soil may influence the absorption of nutrients by the plant and, therefore, its nutritional balance. In this study, we aimed to evaluate the effects of calcium and magnesium silicate (CaSiO3/MgSiO3) on the ability of wheat (Triticum aestivum L.) to utilize silicon and absorb nutrients from soils collected in the state of Paraná, Brazil. The experiment was carried out in a greenhouse using 8-L plastic pots and three types of soil. Treatments were arranged in randomized blocks (3 × 5 factorial design): three soils [Rhodic Acrudox (Ox1), Rhodic Hapludox (Ox2), and Arenic Hapludult (Ult)], five silicate rates (0, 1, 2, 4, and 6 t ha–1 of calcium/magnesium silicate), and four replications were performed. The effects of calcium and magnesium silicate on the concentrations of Si, N, P, K+, Ca2+, Mg2+, S, Cu2+, Zn2+, Fe2+, and Mn2+ within leaves were evaluated. Silicon concentrations in wheat leaves and stems increased with increasing rates of calcium and magnesium silicate applied to the soil. Wheat shoots accumulated averages of 28.2% (Ox1), 60.61% (Ult), and 74.14% (Ox2) of the Si from the silicate applied to the soil. Silicate fertilization increased the amount of Ca+2 and Mg+2 within leaves and reduced the amount of Zn2+ and Mn2+ within leaves. Calcium and magnesium silicate prevented excessive amounts of Mn2+ from being absorbed by wheat, improving the balance in the absorption of this nutrient.

The goal of this study was to assess the dehydration curve and nutritional value of Tifton 85 bermudagrass at two cutting heights from ground level (4 and 8 cm) during 120 days of storage in a closed shed. The dehydration curve was determined using samples from the entire plant at eight different times. The experimental design consisted of randomized blocks with plots subdivided per times and five replicates. The second step consisted of assessing the nutritional value of the stored Tifton 85 bermudagrass in randomized blocks with plots subdivided per times and two treatments per plot: cutting height of four and eight centimeters from the ground, and five different times for the subplots, with five replicates. Dehydration of Tifton 85 bermudagrass at the two heights occurred in 48 hours, considered an ideal time for hay drying. The dry matter content responded quadratically to the time of storage of the two heights, only differing during baling and after 120 days of storage. Crude protein content had a quadratic behavior in the two cutting heights, with the smallest value after 30 days of storage (107.0 g kg-1) and the largest after 90 days (147.8 g kg-1) in the cutting height of eight centimeters. The ether extract exhibited a quadratic behavior in the two cutting heights, only differing after 90 days of storage. The neutral detergent fiber content had linear positive response according to the time of storage, with no difference between the cutting heights. For the neutral detergent fiber content in the two cutting heights, the quadratic regression model was the best fit to the data, differing between the heights after 30 and 60 days of storage. In vitro dry matter digestibility and in vitro cell wall digestibility values of the stored hay were lower than the values obtained at the time of cutting. Cutting performed at four centimeters from the ground was the most suitable for hay production due to higher dry matter production and nutritional value without difference between bailing treatments. Hay storage caused undesirable changes in the nutritional value, especially in fiber content and in vitro digestibility.