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Highly sensitive and selective detection of morphine (MO) was realized by coupling an immunoreaction of an anti-MO monoclonal antibody and a surface-plasmon-resonance (SPR) phenomena. A normorphine-bovine serum albumin (NM-BSA) conjugate prepared as an antigen was immobilized on the Au thin film of sensor chip by physical adsorption. The incident angle of the SPR system using the NM-BSA immobilized chip increased almost linearly with increasing concentration of antibody up to ca. 5 /spl mu/g/ml (ppm). The addition of MO into the antibody solution (5 ppm) was found to reduce the incident angle shift sharply because of the inhibition effect of MO. Based on this inhibiting principle, the present sensor could detect MO very sensitively in the concentration range of 0.1-10 ng/ml (ppb).

P-1894B an inhibitor of collagen prolyl hydroxylase was applied topically to cotton pellet-induced granuloma and to skin lesions in rats. The total hydroxyproline content of the granulomata was significantly reduced by this agent. It also reduced the hydroxyproline content in the tissues surrounding skin lesions during the healing process, with delayed diminution in lesion size. These results suggest that collagen prolyl hydroxylase inhibitor P-1894B may be a suitable agent for topical treatment of hypertrophic scar tissue or keloid.

End-systolic pressure-volume relationship (ESPVR) of the in situ heart in the dog was measured during changes in contractile state and was compared with end-systolic pressure-length (ESPLR) or stress-strain relationship (ESSSR). Circumferential segmental length and wall thickness at the equator and external long and short axis diameters of the left ventricle (LV) were determined sonomicrometrically, and LV volume was calculated by an ellipsoidal model. Circumferential wall stress at the equator was calculated by a very thin shell model. Contractile state was enhanced by an intravenous infusion of dobutamine and was suppressed by propranolol. ESPVR, ESPLR, and ESSSR were determined during a reduction of arterial pressure by occluding temporarily the inferior caval vein (IVC). ESPVR, ESPLR, and ESSSR during changes in end-systolic pressure from 108 +/- 3 to 71 +/- 2 mmHg were linear, irrespective of inotropic states (r greater than 0.92). Slopes of these relationships increased similarly in case of dobutamine and were reduced after propranolol, yet the extrapolated X-axis intercept of ESPVR, ESPLR, and ESSSR remained unchanged. Thus the slope of ESPVR is unique to the inotropic state, and both ESPLR and ESSSR are useful as a substitute for ESPVR when there is no regional wall motion abnormality.

End-systolic pressure-length relationship (ESPLR) was examined in the heterogenously contracting heart in open-chest dogs. Regional length was measured sonomicrometrically in the areas of both the left circumflex coronary artery (LCX) and left anterior descending coronary artery (LAD). ESPLR was linear during a transient reduction in arterial pressure following occlusion of the inferior caval vein (IVC). In group 1 (12 dogs), saline as a control condition (Cont) and isoproterenol (Iso, 0.05 microgram/min) to enhance the regional contractile state were infused into the LCX. Percent shortening in the LCX area increased from 13 to 16% (P less than 0.01) in Iso. The slope (Ees) of ESPLR increased from 57 to 71 mmHg/mm (P less than 0.01) in Iso. The extrapolated X-axis intercept (Lo) of ESPLR was unchanged. In the LAD area, Ees and Lo remained constant. In group 2 (13 dogs), lidocaine (1 mg/min, Lid 1; 5 mg/min, Lid 5) was infused into the LCX to induce mild and severe hypokinesis, respectively. Percent shortening in the LCX area was reduced from 14 to 8% (P less than 0.01 vs. Cont) in Lid 1 and 5% (P less than 0.01 vs. Cont, P less than 0.05 vs. Lid 1) in Lid 5. Ees tended to decrease in Lid 1 but to increase in Lid 5 (statistically not significant).(ABSTRACT TRUNCATED AT 250 WORDS)

The selective hydrogenation of 1,5,9-cyclododecatriene to cyclododecene by use of a series of complex catalysts of the type [Co(CO)3PR3]2(R = alkyl, aryl) in homogeneous phase has been studied with special interest in the relations between the σ-donor strength of used tert-phosphine and catalyst activity. The complex containing trialkylphosphines are stable enough to be recovered after the reaction and exerted almost complete selectivity. The mechanism of the selective hydrogenation is postulated in view of the effect of σ-donor ligand which modifies the equilibrium condition between σ- and π-allyl type reaction-intermediates produced by the addition of metal hydride to diene and also from in view of the configurational factors which may influence the stability of these intermediates.