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Wuzi Yanzong Pill (WZYZP), a classic traditional Chinese medicine prescription, has been widely used to alleviate spermatogenesis dysfunction for hundreds of years. However, the molecular mechanisms of WZYZP treatment for spermatogenesis dysfunction remain limited. Here, our results showed that WZYZP significantly improved spermatogenesis and testicular energy metabolism. The 16S rDNA sequencing showed that WZYZP improved gut microbiota dysbiosis, including increased relative abundances of Lactobacillus with the capability of metabolizing Trp. The depletion of gut microbiota by antibiotics suppressed the ability of WZYZP to improve spermatogenesis dysfunction. The untargeted and targeted metabolomics results showed that WZYZP increased Trp metabolites especially aryl hydrocarbon receptor (AhR) ligands. Moreover, WZYZP modulated the AhR/AMPK pathway to improve the expression of spermatogenesis-related genes. The correlation analysis showed that gut microbiota was significantly correlated with Trp metabolites, and Trp metabolites were closely related to spermatogenesis. Overall, the results demonstrated that WZYZP could regulate gut microbiota, and the gut microbial Trp metabolites further act on testicular energy metabolism, thereby indirectly improving spermatogenic dysfunction. Our study provides a novel research strategy for complementary and alternative medicine in male infertility from the perspective of gut microbial metabolites.

Protein-based emulsion gels are an ideal delivery system due to their unique structure, remarkable encapsulation efficiency, and tunable digestive behavior. Freshwater mussel (Solenaia oleivora) protein isolate (SoPI), an emerging sustainable protein with high nutritional value, possesses unique value in the development of functional foods. Herein, composite emulsion gels were fabricated with SoPI and κ-carrageenan (κ-CG) for the delivery of curcumin. SoPI/κ-CG stabilized emulsions possessed a high encapsulation efficiency of curcumin with a value of around 95%. The addition of κ-CG above 0.50% facilitated the emulsion gel formation and significantly improved the gel strength with 1326 g. Furthermore, the storage and digestive stability of curcumin were significantly improved as the κ-CG concentration increased. At 1.50% κ-CG, around 80% and 90% curcumin remained after 21-day storage at 45 °C and the 6 h in vitro gastrointestinal digestion, respectively. The addition of 0.50% κ-CG obtained the highest bioaccessibility of curcumin (~60%). This study illustrated the potential of SoPI emulsion gels as a carrier for stabilizing and delivering hydrophobic polyphenols.

In a tritium-breeding blanket of a fusion reaction, helium, used as a tritium-purging gas, will purge the tritium breeder pebble beds to extract the tritium in blanket. The purge gas flow characteristics will affect the tritium extraction efficiency. The effect of the fixed wall on the pebble packing structures and purge gas flow characteristics was investigated by combining the discrete element method (DEM) and computational fluid dynamics (CFD) method. The results indicate that the fixed wall leads to a regular packing of the pebbles adjacent to the fixed wall in association with drastic fluctuations in the porosity of the pebble bed, which can affect the purge gas flow behaviors. Further analyses of helium flow behaviors show that the helium pressure in the pebble bed decreases in a linear manner along the flow direction, whereas the pressure drop gradient of helium increases gradually with an increase in the packing factor. The reduction in porosity in the pebble bed leads to a notable escalation in helium flow velocity. Concerning the direction perpendicular to the helium gas flow, the evolution of the cut-plane averaged velocity of helium is similar to that of the porosity, except in the region immediately adjacent to the wall. The pressure drop and flow characteristics obtained in this study can serve as input for the thermohydraulic analysis of the tritium blowing systems in the tritium-breeding blanket of a fusion reactor.

In order to enhance the durability of fuel cell systems in fuel cell hybrid electric vehicles (FCHEVs), researchers have been dedicated to studying the degradation monitoring models of fuel cells under driving conditions. To predict the actual degradation factors and lifespan of fuel cell systems, a semi-empirical and semi-physical degradation model suitable for automotive was proposed and developed. This degradation model is based on reference degradation rates obtained from experiments under known conditions, which are then adjusted using coefficients based on the electrochemical model. By integrating the degradation model into the vehicle simulation model of FCHEVs, the impact of different fuel cell sizes and dynamic limitations on the efficiency and durability of FCHEVs was analyzed. The results indicate that increasing the fuel cell stack power improves durability while reducing hydrogen consumption, but this effect plateaus after a certain point. Increasing the dynamic limitations of the fuel cell leads to higher hydrogen consumption but also improves durability. When considering only the rated power of the fuel cell, a comparison between 160 kW and 100 kW resulted in a 6% reduction in hydrogen consumption and a 10% increase in durability. However, when considering dynamic limitation factors, comparing the maximum and minimum limitations of a 160 kW fuel cell, hydrogen consumption increased by 10%, while durability increased by 83%.

To further improve the performance of the Proton Exchange Membrane Fuel Cell (PEMFC), in this paper, we designed a blocked flow channel with trapezoidal baffles, and geometric parameters of the baffle were optimized based on CFD simulation, Artificial Neural Network (ANN), and single-objective optimization methods. The analysis of velocity, pressure, and oxygen distribution in the cathode flow channel shows that the optimized trapezoidal baffle can improve oxygen transport during the reaction. The comparison of the optimization model with the straight flow channel model and the rectangular baffle model shows that the power density of the optimized model is 4.0% higher than that of the straight flow channel model at a voltage of 0.3 V, and the pressure drop is only 37.83% of that of the rectangular baffle model. For on-road PEMFC with a voltage of 0.6 V, the influence of pump power is significant, and the optimized trapezoidal baffle model has a net power increase of 1.47% compared to the rectangular baffle model at 50% pump efficiency and 3.94% at 30% pump efficiency.

In order to meet the increasing pollutants discharge standard, the selective catalytic reduction (SCR) module in the diesel engine after-treatment system is an important means to reduce nitrogen oxide (NOx) emissions. SCR systems are prone to urea crystallization at lower temperatures, especially during the cold-start conditions of diesel engines. In this study, we use the diesel engine after-treatment system test bench to obtain the boundary parameter of the simulation modules, and the urea crystallization risk assessment model of the diesel SCR system is established. Comparing the computational fluid dynamics (CFD) results with the test bench results, it is shown that the predicted urea film distribution of the assessment model is in good agreement with the experimental results. In order to clarify the various factors that affect the urea crystallization risk, this paper conducts a simulation analysis on a nozzle and mixer structure and operating parameters. The CFD results indicate that the increase in urea spray time will increase the maximum urea film thickness on the SCR system mixer surface. Exhaust temperature is the most important influencing factor. When the diesel engine exhaust temperature increases from 190 °C to 300 °C, the maximum urea film thickness decreases by 32 and the urea film mass accumulation decreases by 5%. Exhaust flow has a small impact on urea crystallization risk. When the exhaust flow increases from 300 kg/h to 600 kg/h, the maximum urea film thickness decreases by 39% and the urea film mass accumulation decreases by about 1%. In addition, urea spray rate, nozzle numbers, spray angle, and spray cone angle are also factors that affect urea crystallization risk.

To achieve diesel engine ultra-low nitrogen oxide emission, light-off selective catalyst reduction (LO-SCR) has been suggested for better performance with lower exhaust temperature. An electric heater upstream of the exhaust aftertreatment system was applied to significantly decrease the NOx emission at a low exhaust temperature. With a 7.2 kW electric heater coupled with LO-SCR, the NOx emission during 200~500 s of the world harmonized transient cycle (WHTC) decreased from 282.6 ppm to 61.5 ppm, which is a decrease of 45%. Application of an upstream diesel oxidation catalyst (DOC) decreased the NOx emission by 63% at the same interval at the cost of worse cold-start performance. The urea input was also adjusted to avoid NOx emission during the latter part of the WHTC.

One of the most productive ecosystems in the world, mangroves are susceptible to cold stress. However, there is currently insufficient knowledge of the adaptation mechanisms of mangrove plants in response to chilling stress. This study conducted a comparative analysis of transcriptomics and metabolomics to investigate the adaptive responses of Kandelia obovata (chilling-tolerant) and Avicennia marina (chilling-sensitive) to 5 °C. The transcriptomics results revealed that differentially expressed genes (DEGs) were mostly enriched in signal transduction, photosynthesis-related pathways, and phenylpropanoid biosynthesis. The expression pattern of genes involved in photosynthesis-related pathways in A. marina presented a downregulation of most DEGs, which correlated with the decrease in total chlorophyll content. In the susceptible A. marina, all DEGs encoding mitogen-activated protein kinase were upregulated. Phenylpropanoid-related genes were observed to be highly induced in K. obovata. Additionally, several metabolites, such as 4-aminobutyric acid, exhibited higher levels in K. obovata than in A. marina, suggesting that chilling-tolerant varieties regulated more metabolites in response to chilling. The investigation defined the inherent distinctions between K. obovata and A. marina in terms of signal transduction gene expression, as well as phenylpropanoid and flavonoid biosynthesis, during exposure to low temperatures.

A novel sulfonic-functionalized MnFe2O4 (MnFe2O4-HSO3) Fenton nanocatalyst was prepared and tested for the degradation of three antibiotics from different categories. The effect of category of the antibiotic was investigated and the obtained results indicated that the degradation rate was the highest for erythromycin, lower for tetracycline and the lowest for amikacin. Moreover, the role of -HSO3 was investigated by a series of comparative experiments between MnFe2O4 and MnFe2O4-HSO3. The introduction of -HSO3 prevented the leaching of metals and promoted the decomposition of H2O2 into •OH radicals. Finally, the excellent stability of MnFe2O4-HSO3 was verified.

As a powerful solution for heat dissipation in data centers, chip-level cooling continues to capture escalating attention in research and application domains. To accurately analyze system performance, identify potential avenues for system optimization, and inform future practical applications, we developed a steady-state, one-dimensional mathematical model for a novel pump-driven chip-level two-phase cooling system (PCTCS). This model was constructed based on our previous study and was confirmed against existing experimental data. Our simulations scrutinized PCTCS performance under default conditions and investigated the effects of key parameters, such as refrigerant type, condenser vertical positioning, and cooling water temperature. Results showed that the system could manage an 80 W power output from each CPU while maintaining CPU temperatures around 79 °C at a cooling water temperature of 45 °C. We discovered the choice of refrigerant had a significant impact on performance, with R32 outperforming R134a and R113. While the vertical position of the condenser influenced the PCTCS’s internal parameters, its overall impact on system performance was negligible. Moreover, provided the chip temperature remained within a safe range, our study found that increasing the cooling water temperature improved the energy efficiency ratio of the refrigerant pump and reduced the temperature difference between the chips and the cold source.

Developing effective therapies and medicines to conquer nonalcoholic steatohepatitis (NASH) is of great significance for public health and is faced with a major challenge. The activation of the thyroid hormone receptor agonist THRβ could be regulated by target drugs that has brought huge potential to the treatment of NASH. In this work, pyridazinone compound YWS01125 was synthesized for the first time. In this study, an ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method for YWS01125 determination was established, and the pharmacokinetics of YWS01125 was evaluated. The half-life values (t1/2)of three different doses of YWS01125 was 189.12 ± 95.27, 152.64 ± 37.98, and 181.95 ± 64.25 min, respectively, and the tissue distribution studies demonstrated that YWS01125 was quickly distributed to various tissues. With successful application in the pharmacokinetics study of YWS01125, the UPLC-MS/MS method has shown characteristics of high sensitivity, rapidity, and good selectivity.

The effects of mono-, dual- and tri-frequency ultrasound-assisted thawing (UAT) on the physicochemical quality, water-holding capacity, moisture migration and distribution and myofibrillary structure of frozen large yellow croaker (Larimichthys crocea) were detected. The results indicated that multifrequency UAT treatment significantly increased the thawing rate, maintained the stability of myofibrils and reduced the lipid oxidation. The multifrequency UAT samples had better water-holding capacity (higher water-holding capacity values, lower thawing loss and cooking loss) and physicochemical quality (higher hardness, springiness, resilience, chewiness and lower total volatile basic nitrogen (TVB-N) values, thiobarbituric acid reactive substances (TBARS) values), higher immobilized water content, and lower free water content. Therefore, the results provide a further understanding of the quality stability of frozen large yellow croaker treated by the multifrequency UAT.

Aiming at nonlinear flow in fractured porous media, based on the finite volume method, the discrete equations of Darcy flow in porous and Forchheimer flow in fracture were derived, and a solution method for coupling flow is proposed. The flow solution by the proposed method for single fracture and intersecting fracture is verified against Frih’s solution. Based on this method, nonlinear flow behavior for fractured rock deep-buried tunnels under high water heads was discussed. The results show that the hydraulic gradient of surrounding rock is characterized by “large at the bottom and small at the top”, with a maximum difference of 2.5 times. Therefore, the flow rate at the bottom of the tunnel is greater than that at the top. The fracture flow rate along the flow direction is also greater than that in the vertical flow direction, with a maximum difference of 60 times. The distribution homogeneity and density of fracture are the most important factors that affect the hydraulic behavior of fractured rock tunnels. The more fractures concentrated in the direction of water pressure and the greater the density, the greater the surrounding rock conductivity and the greater the flow rate of the tunnel. Under this condition, the water-inflow accident of the tunnel would be prone to occur. The research results provide a reference for the waterproof design and engineering practice of fractured rock tunnels.

The modified Cam-clay model (MCC model) is capable of representing the consolidation process of the soil under a complex stress path and thus is extensively adopted in the numerical analysis of excavation engineering. For reliability problems of ground surface settlement of excavation, minimal attention has been paid to investigating the effect of spatial variable MCC model parameters on the settlement during the staged construction of the excavation. Based on laboratory tests and sensitivity analysis, this study launched the probabilistic transient hydro-mechanical coupling analysis of excavation settlement considering spatial variable deformation parameters (λ and κ) using an MCS-FORM hybrid approach. The results show that: (1) Both the deformation parameters have a positive relationship with the settlement, and κ has a more significant effect than λ. (2) The observed maximum settlement and the location with maximum settlement follow a log-normal distribution. (3) An increasing COV of parameters leads to an enhanced surface settlement, expansion of the significant influence region, and decreased reliability. (4) The reliability index is greatly enhanced with the delimited controlled standard value of the surface settlement, Hcon, and it decreases significantly at the preliminary stage and then decreases progressively until stable during excavating. Overall, adopting the deterministic analysis without considering the spatial variability of MCC parameters leads to underestimating the risk due to the settlement and the significant influence region.

Root foraging enables plants to obtain more soil nutrients in a constantly changing nutrient environment. Little is known about the adaptation mechanism of adventitious roots of plants dominated by asexual reproduction (such as tea plants) to soil potassium heterogeneity. We investigated root foraging strategies for K by two tea plants (low-K tolerant genotype “1511” and low-K intolerant genotype “1601”) using a multi-layer split-root system. Root exudates, root architecture and transcriptional responses to K heterogeneity were analyzed by HPLC, WinRHIZO and RNA-seq. With the higher leaf K concentrations and K biological utilization indexes, “1511” acclimated to K heterogeneity better than “1601”. For “1511”, maximum total root length and fine root length proportion appeared on the K-enriched side; the solubilization of soil K reached the maximum on the low-K side, which was consistent with the amount of organic acids released through root exudation. The cellulose decomposition genes that were abundant on the K-enriched side may have promoted root proliferation for “1511”. This did not happen in “1601”. The low-K tolerant tea genotype “1511” was better at acclimating to K heterogeneity, which was due to a smart root foraging strategy: more roots (especially fine roots) were developed in the K-enriched side; more organic acids were secreted in the low-K side to activate soil K and the root proliferation in the K-enriched side might be due to cellulose decomposition. The present research provides a practical basis for a better understanding of the adaptation strategies of clonal woody plants to soil nutrient availability.

NRT1/PTR FAMILY (NPF) genes are characterized as nitrate and peptide transporters that played important roles in various substrates transport in plants. However, little is known about the NPF gene in tea plants. Here, a total of 109 CsNPF members were identified from the tea plant genome, and divided into 8 groups according to their sequence characteristics and phylogenetic relationship. Gene structure and conserved motif analysis supported the evolutionary conservation of CsNPFs. Many hormone and stress response cis-acting elements and transcription factor binding sites were found in CsNPF promoters. Syntenic analysis suggested that multiple duplication types contributed to the expansion of NPF gene family in tea plants. Selection pressure analysis showed that CsNPF genes experienced strong purifying selective during the evolution process. The distribution of NPF family genes revealed that 8 NPF subfamilies were formed before the divergence of eudicots and monocots. Transcriptome analysis showed that CsNPFs were expressed differently in different tissues of the tea plant. The expression of 20 CsNPF genes at different nitrate concentrations was analyzed, and most of those genes responded to nitrate resupply. Subcellular localization showed that both CsNPF2.3 and CsNPF6.1 were localized in the plasma membrane, which was consistent with the characteristics of transmembrane proteins involved in NO3- transport. This study provides a theoretical basis for further investigating the evolution and function of NPF genes.

TiO2 powder was firstly synthesized and carbon fiber was secondly prepared via the carbonization of polyaniline fiber, and TiO2/carbon fiber composites were lastly synthesized via a simple method at room temperature. The prepared samples are evidently investigated by X-ray powder diffraction, scanning electron microscopy, energy dispersive spectroscopy, ultraviolet-visible diffuse reflectance spectroscopy, photoluminescence spectrum, and X-ray photoelectron spectroscopy, respectively. Using the monochromatic light of ultraviolet, the photocatalytic activity of the TiO2/CF composites was accurately evaluated with respect to the degradation of an aqueous dye (methylene blue) solution. The relationship between the photocatalytic degradation of methylene blue dye and its ratio, contact time, and the amount of catalyst was studied. The kinetics and mechanisms of degradation were discussed. The results show that TiO2/CF composites have good photocatalytic activity and stability. The TiO2/CF2/1 composite was used in effective photocatalytic degradation of methylene blue, the weight ratio of TiO2 to carbon fiber was 2:1, and the degradation rate was obtaining up to 97.7% of degradation during 120 min of reaction. The photocatalytic stability of TiO2/CF composites was dependent on the stability of their structure. After 5 repeated uses, the composite TiO2/CF2/1 still exhibited rather high activity toward the degradation of methylene blue, where the decolorization efficiency of methylene blue achieved 92% and the loss of activity was negligible. Based on radical trapping experiments, the mechanism of TiO2/CF composites on photocatalytic degradation of methylene blue is proposed, which could explain the enhanced photocatalytic activity of the composites better. Superoxide radicals, photogenerated holes, and photogenerated electrons were the main active substances for methylene blue degradation.

As rock fractures caused by micro-seismic events has potential safety hazards to underground workers, it is often necessary to accurately locate the micro-seismic source for hidden danger investigation. Micro-seismic data are generated in complex underground environments which are significantly affected by random noise. These data greatly influence subsequent micro-seismic source location, energy estimation, and disaster monitoring. In this paper, a new denoising method based on Time-Frequency Peak Filtering (TFPF) and Complete Ensemble Empirical Mode Decomposition with Adaptive Noise (CEEMDAN) is proposed for micro-seismic data. Micro-seismic data are decomposed into several Intrinsic Mode Functions (IMFs) by CEEMDAN. Then, discriminant factors are used to determine which IMF needs to be denoised by TFPF. Finally, the denoised result is reconstructed by inverse CEEMDAN. By comparing and analyzing different entropies, Fuzzy Entropy (FE) is selected as the best discriminant factor. The CEEMDAN-FE-TFPF denoising method can effectively avoid the influence of fixed window length of the conventional TFPF method. The effectiveness and superiority of this method are verified by experiments of synthetic and actual data.

With intensification of urbanization throughout the world, food security is being threatened by the population surge, frequent occurrence of extreme climate events, limited area of available cultivated land, insufficient utilization of urban space, and other factors. Determining the means by which high-yielding and high-quality crops can be produced in a limited space is an urgent priority for plant scientists. Dense planting, vertical production, and indoor cultivation are effective ways to make full use of space and improve the crop yield. The results of physiological and molecular analyses of the model plant species Arabidopsis thaliana have shown that the plant response to shade is the key to regulating the plant response to changes in light intensity and quality by integrating light and auxin signals. In this study, we have summarized the major molecular mechanisms of shade avoidance and shade tolerance in plants. In addition, the biotechnological strategies of enhancing plant shade tolerance are discussed. More importantly, cultivating crop varieties with strong shade tolerance could provide effective strategies for dense planting, vertical production, and indoor cultivation in urban agriculture in the future.

Logic gates, as one of the most important basic units in electronic integrated circuits (EICs), are also equally important in photonic integrated circuits (PICs). In this study, we proposed a non-volatile, ultra-compact all-photonics logic gate. The footprint is only 2 μm × 2 μm. We regulate the phase change of optical phase change materials(O-PCMs) Sb2Se3 to switch the function of the logic gate. The Sb2Se3 possess a unique non-volatile optical phase change function; therefore, when Sb2Se3 is in the crystalline or amorphous state, our device can work as XOR gate or AND gate, and our designed logic ‘1’ and logic ‘0’ contrasts reach 11.8 dB and 5.7 dB at 1550 nm, respectively. Compared with other traditional optical logic gates, our device simultaneously has non-volatile characteristics, tunability, and additionally an ultra-small size. These results could fully meet the needs of fusion between PICs and EICs, and developing truly chip-scale optoelectronic logic solution.

Nano-scaled Fe3O4 were synthesized using solvothermal synthesis, and amino-modification of nano-scaled Fe3O4 was conducted with APTES. With Fe3O4-NH2 and Zn2+ as the metal centers, 2,6-pyridinedicarboxylic acids as the organic ligand, and using the one-step ultrasonic-assisted method, a new magnetic zinc pyridinedicarboxylic acid nanocomposite (Zn-(PDC)2@Fe3O4) was synthesized. The structure, composition and morphology of the products were characterized using methods such as X-ray single crystal diffraction, Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction, and scanning electron microscope. The results showed that the Zn-(PDC)2 was almost an octahedron, and the Zn-(PDC)2@Fe3O4 looked like a nanoflower. The mimetic peroxidase properties of Zn-(PDC)2@Fe3O4 were studied with H2O2 solution and TMB solution as the substrates, and the results showed that: the Michaelis constant of H2O2 by Zn-(PDC)2@Fe3O4 was: Km = 0.411 mM, the maximum reaction rate: vmax = 3.440 × 10−8 M s−1; the Michaelis constant of TMB by Zn-(PDC)2@Fe3O4 was: Km = 0.189 mM, and the maximum reaction rate: vmax = 2.419 × 10−8 M s−1, both of which were lower than those of H2O2 and TMB by Fe3O4 and Horseradish Peroxidase (HRP). Based on the reduction of the absorbance of original solution due to the consumption of H2O2 in oxidation-reduction reaction between SO32− and H2O2 under acid conditions, a new method for determining the content of SO32− was established. The linear range of SO32− was 8 × 10−7∼8 × 10−5 mol/L, the detection limit of SO32− was 8 × 10−8 mol/L, and the RSD of SO32− was 2.7–9.2%. Keywords: Magnetic nanocomposite, Mimetic peroxidase, SO32−, Colorimetric analysis

In this paper, the high-concentration aqueous dispersion N-doped anatase TiO2 is prepared based on low temperature non-aqueous solvent-thermal method by using TCl4 and NH4Cl as titanium source and nitrogen source respectively. From TEM image, we can learn that the particle is composed of spindle-shaped particles with the size from 8 to 15 nm. According to the XRD and XPS spectra of N-doped TiO2, it can be seen that, during the synthesis process, N atoms permeate into the TiO2 lattice, and the impurity level can be formed within the band gap of TiO2. The impurity level can extend to absorb UV and the visible light. As for N-doped TiO2 nanoparticle of XPS spectrum, ethanol is used as solvent to form carbonaceous species on the surface of TiO2. The carbonaceous species in visible light can excite electrons towards the TiO2 conduction band, so as to increase the number of electrons in the conduction band under visible light to improve the catalytic activity of TiO2 which possesses the feature of high catalytic activity under UV–visible light. Keywords: TiO2 nanoparticle, Highly aqueous dispersion, Visible light photocatalytic activity

Filling mining plays an important role in controlling surface subsidence. To study the movement of overburdened rock in filling mining under thick loose layers, a numerical simulation combing field measurement in CT30101 working face in the Mahuangliang coal mine was tested. The results show that different filling rates and filling body strength have different influences on roof and surface movement. The filling rate has a greater impact, which is the main control factor. The filling stress and roof tensile stress decrease gradually with roadway filling. The filling body stress and roof tensile stress in the first and second rounds are far greater than in the fourth round. After the completion of filling, the first and second round of filling bodies mainly bear the overburden, and the total deformation of the surrounding rock of the main transport roadway is very small, and therefore the displacement of the overburdened rock is controllable. The field monitoring results also show that the overburdened rock became stable after several fillings rounds. Combing the numerical modeling and field tests results, this study can be a guideline for similar geological conditions especially for coal mining under thick loose layers and thin bedrock.

As contaminated water increases due to environmental pollution, the need for excellent water treatment is increased, and several studies have reported the polyvinylidene fluoride (PVDF)-based water treatment membranes. However, the PVDF membrane has several problems such as low filtration performance, fouling resistance, and difficulty in precisely controlling the morphology of the pores and hydrophilicity. Therefore, we newly produced a water treatment PVDF membrane containing graphene oxide (GO) and multi-walled carbon nanotubes (MWCNTs) to improve the filtration performance. Surface properties of the fabricated membrane such as morphology, and size of pores, hydrophilicity, and water flux of the membrane were investigated. Additionally, the performance of these membrane filters was evaluated for free residual chlorine, turbidity, chromaticity, magnesium, sulfate, and particulates class 1 according to drinking water management act criteria, respectively. A performance improvement of at least 108.37% was observed compared to the Pure PVDF filter module and anti-fouling effects due to the functional groups of GO and MWCNTs. These results reveal that proposed membrane can accelerate the development of various water filtration applications.

We developed a complex three-dimensional (3D) multilayer deposition method for the fabrication of single-walled carbon nanotubes (SWCNTs) using vacuum filtration and plasmonic carbonization without lithography and etching processes. Using this fabrication method, SWCNTs can be stacked to form complex 3D structures that have a large surface area relative to the unit volume compared to the single-plane structure of conventional SWCNTs. We characterized 3D multilayer SWCNT patterns using a surface optical profiler, Raman spectroscopy, sheet resistance, scanning electron microscopy, and contact angle measurements. Additionally, these carbon nanotube (CNT) patterns showed excellent mechanical stability even after elastic bending tests more than 1000 times at a radius of 2 mm.

The effect of changes in non-solvent coagulation bath temperature on surface properties such as morphology and hydrophilicity were investigated in multi-walled carbon nanotubes (MWCNTs) and graphene oxide (GO)-based polyvinylidene fluoride (PVDF) membranes. The properties of pores (size, shape, and number) as well as membrane hydrophilicity were investigated using field emission scanning electron microscopy, Raman spectroscopy, optical microscopy, water contact angle, and water flux. Results showed that the pore size increased with an increase in coagulation temperature. The hydrophilic functional groups of the added carbon materials increased the solvent and non-solvent diffusion rate, which significantly increased the number of pores by 700% as compared to pure PVDF. Additionally, these functional groups changed the hydrophobic properties of pure PVDF into hydrophilic properties.

Increasing evidence indicates that human exposure to inorganic arsenic causes cutaneous diseases and skin cancers. Nuclear factor erythroid 2-like 1 (NRF1) belongs to the cap “n” collar (CNC) basic-region leucine zipper (bZIP) transcription factor family and regulates antioxidant response element (ARE) genes. The human NRF1 gene is transcribed into multiple isoforms, which contain 584, 616, 742, 761, or 772 amino acids. We previously demonstrated that the long isoforms of NRF1 (i.e., NRF1-742, NRF1-761 and NRF1-772) are involved in the protection of human keratinocytes from acute arsenic cytotoxicity by enhancing the cellular antioxidant response. The aim of the current study was to investigate the roles of NRF1-742 and NRF1-772 in the arsenic-induced antioxidant response and cytotoxicity. We found that overexpression of NRF1-742 or NRF1-772 in human HaCaT keratinocytes decreased susceptibility to arsenic-induced apoptosis and cytotoxicity. In addition, we characterized the different protein bands observed for NRF1-742 and NRF1-772 by western blotting. The posttranslational modifications and nuclear translocation of these isoforms differed and were partially affected by arsenic exposure. Antioxidant protein levels were increased in the NRF1-742 and NRF1-772-overexpressing cell lines. The upregulation of antioxidant protein levels was partly due to the translation of nuclear factor erythroid 2-like 2 (NRF2) and its increased nuclear transport. Overall, overexpression of NRF1-742 and NRF1-772 protected HaCaT cells from arsenic-induced cytotoxicity, mainly through translational modifications and the promotion of antioxidant gene expression.

Adventitious root (AR) formation is essential for the successful propagation of Camellia sinensis and auxins play promotive effects on this process. Nowadays, the mechanism of auxin-induced AR formation in tea cuttings is widely studied. However, a lack of global view of the underlying mechanism has largely inhibited further studies. In this paper, recent advances including endogenous hormone changes, nitric oxide (NO) and hydrogen peroxide (H2O2) signals, secondary metabolism, cell wall reconstruction, and mechanisms involved in auxin signaling are reviewed. A further time course analysis of transcriptome changes in tea cuttings during AR formation is also suggested to deepen our understanding. The purpose of this paper is to offer an overview on the most recent developments especially on those key aspects affected by auxins and that play important roles in AR formation in tea plants.

Laser welding has been increasingly utilized to manufacture a variety of components thanks to its high quality and speed. For components with complex shapes, the welding position needs be continuously adjusted during laser welding, which makes it necessary to know the effects of the welding position on the quality of the laser welds. In this paper, the weld quality under two (flat and horizontal) welding positions were studied comparatively in the laser welding of Ti6Al4V titanium alloy, in terms of weld profiles, process porosity, and static tensile strengths. Results show that the flat welding position led to better weld profiles, less process porosity than that of the horizontal welding position, which resulted from the different actions of gravity on the molten weld metals and the different escape routes for pores under different welding positions. Although undercuts showed no association with the fracture positions and tensile strengths of the welds, too much porosity in horizontal laser welds led to significant decreases in the strengths and specific elongations of welds. Higher laser powers and travel speeds were recommended, for both flat and horizontal welding positions, to reduce weld porosity and improve mechanical properties.