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The work aimed to evaluate the effect of nitrogen dose management on growth responses and physiological performance of barley seeds. Two barley cultivars (BRS Brau and BRS Caue) and four nitrogen doses were used. The experimental design was randomized blocks in a 2 X 4 factorial scheme (cultivar BRS Brau and BRS Caue x nitrogen doses 120 kg ha-1,1 50 kg ha-1, 180 kg ha-1 and 210 kg ha-1). Successive collections were performed every fourteen days of primary data for the establishment of growth analysis. The variables analyzed were total dry matter, dry matter production and relative growth rate, assimilated partition and seed electrical conductivity. Increasing nitrogen doses causes temporal-quantitative difference in growth, partition and in the accelerated aging test of barley seeds. The 150 kg ha-1 N dose resulted in total dry matter over time (Wt) superiority for both cultivars evaluated. The variable dry matter production rate achieved increase for the 150 kg ha-1 N dose at 70 days after emergency (DAE) for BRS Brau and at 56 DAE for BRS Caue, supporting Wt. The dose of 180 kg ha-1 N and 150 kg ha-1 N result in higher the relative growth rate for BRS Brau and BRS Caue.