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Leishmania tarentolae has been used to produce recombinant intracellular and secreted proteins for their easy handling and posttranslational modifications. Filamentous acid phosphatase is a multimeric protein complex composed of many subunits assembled in a linear highly glycosylated filament, which is secreted in vast amounts into the culture supernatant via the flagellar pocket of Leishmania mexicana promastigotes. This suggested that the protein could be used as a carrier for other proteins for easy purification to generate a protein complex decorated with multiple SAG1 subunits suitable for immunisation. Surface Antigen1 protein of a Toxoplasma gondii has an immunodominant structure that is involved in binding to host cells. Previous studies used this surface protein for vaccination for its immunological importance for triggering a type 1 immune response in the host. This study aims to determine the production of recombinant filamentous protein carried subunits of the surface protein of Toxoplasma gondii for vaccination purposes. Leishmania codon-optimised SAG1 was cloned as a fusion construct into pLEXSY-ble2.1 and introduced into Leishmani. tarentolae to generate recombinant cell lines expressing of a filamentous fusion protein called SAP2SAG1. PCR confirmed the correct integration into the small ribosomal subunit RNA gene locus of Leishmania tarentolae. Immunofluorescences and Immunoblot analyses were used to detect the fusion protein in the sediment of culture supernatants of recombinant L. tarentolae promastigotes after purification by ultracentrifugation. The yield of purified protein was low that suggested further investigations of other methods for scaling large production of secreted protein.

To conduct the influence of salinomycin & anticoccidial vaccine onpathological changes in intestine of broiler chickens experimentalinfected with Eimeria spp. By using 40 broiler chicks divided randomlyto four groups( 10 chicks of each group ) First group vaccinated withanticoccidial vaccine (coccivac) at 8 days of age in drinking water whilethe second group feeded salinomycin in concentration 60ppm from oneday old ,the third group left non vaccinated nor given salinomycin as acontrol group at(26 days )of age chicks in that three groups infected withdifferent species of Eimeria spp .with high dose (744x74³) Oocyst /chickby group inoculation & the fourth group consider negative control notvaccinated or treated .At (33 days)of chicks age all the chicks were killedfor measuring lesion score of intestine which their results +1.75 , +3, +4with mortality rate 0%,10%,20% for vaccinated ,salinomycine treated &control groups respectively then took samples for Histopathologicalchanges the results pointed high pathological changes in intestine ofpositive control group with sever necrosis & degeneration of epitheliawith sever tissue damage companied with maturation first & secondgeneration sporozoite in the endothelial layer of intestine. While thepathological changes was less sever in salinomycine treated group withnecrosis & degeneration of epithelial layers accompanied with exist ofsporozoite in endothelial layer of intestine . while the vaccinated groupshowed no clear pathological changes in intestine except hyperplasia ofglobal cells & infiltration of lymphocytes cells in the layers of intestine ,it was concluded that the vaccine which was given to chicks provide goodprotection & decrease dangerous of coccidiosis infection .