Search

Your search keyword '"Cyrielle Fournier"' showing total 2 results
Start Over Author "Cyrielle Fournier" Topic chemistry
2 results on '"Cyrielle Fournier"'

1. Cellular and Molecular Impact of Vitrification Versus Slow Freezing on Ovarian Tissue

Author

Jacqueline Lornage, Bruno Salle, Cyrielle Fournier, M. Benchaib, Claire Santamaria, Muriel Rabilloud, Elsa Labrune, and Pauline Jaeger

Subjects
0206 medical engineering, Biomedical Engineering, Medicine (miscellaneous), Apoptosis, Bioengineering, Ovary, 02 engineering and technology, Tissue Culture Techniques, Andrology, 03 medical and health sciences, Ovarian Follicle, Freezing, Gene expression, medicine, Animals, Vitrification, 030304 developmental biology, Cryopreservation, Slow freezing, 0303 health sciences, Sheep, Chemistry, Gene Expression Profiling, Ovarian tissue, Histology, 020601 biomedical engineering, medicine.anatomical_structure, Gene Expression Regulation, Female, Tissue Preservation
Abstract

Objective: To evaluate a vitrification protocol from histology to gene expression to slow freezing. Methods: Ovaries from 12 prepubertal ewes. The same ovary was cut into fragments, studied fresh, ...

Published
2020

2. A New Bioreactor to Promote Follicular Growth With or Without Activin A

Author

Cynthia Jovet, Jacqueline Lornage, Antoine Gavoille, Elsa Labrune, David Laurent, Nicolas Morel-journel, Eloise Fraison, Alexandra Montembault, Bruno Salle, and Cyrielle Fournier

Subjects
Activin a, endocrine system, Chemistry, Follicular phase, Bioreactor, Cell biology
Abstract

Background: The aim of the present study was to evaluate the effect of Activin A on the activation of in vitro folliculogenesis of human ovarian tissues with or without our new three-dimensional structure (3D-structure). Methods: Five fresh ovarian human tissues were cultured in vitro in 4 groups with 100ng/mL Activin A or without Activine A and within or without a 3D-structure for 20 or 22 days of in vitro culture. Follicular density and quality were evaluated, and follicular diameters were measured. Estradiol secretion was quantified. Proliferation and apoptosis through immunostaining were performed.Results: The proportion of primordial follicles was significantly reduced, and the proportion of primary and secondary follicles was significantly increased in all four groups (p0.05). The 3D-structure increased the density of primary follicles and decreased the estradiol secretion (pConclusion: The presence of Activin A did not seem to play a key role in in vitro folliculogenesis activation. However, the results may indicate that the 3D-structure could be more physiological and could prevent a pejorative in vitro folliculogenesis flare-up.

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results