1,032 results on '"CHAO HUANG"'
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2. 2FAKA-C/S: A Robust Two-Factor Authentication and Key Agreement Protocol for C/S Data Transmission in Federated Learning
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Chao Huang, Bin Wang, Zhaoyang Bao, and Wenhao Qi
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federated learning (FL) ,client–server ,authentication ,hash-chain ,elliptic curve cryptography (ECC) ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
As a hot technology trend, the federated learning (FL) cleverly combines data utilization and privacy protection by processing data locally on the client and only sharing model parameters with the server, embodying an efficient and secure collaborative learning model between clients and aggregated Servers. During the process of uploading parameters in FL models, there is susceptibility to unauthorized access threats, which can result in training data leakage. To ensure data security during transmission, the Authentication and Key Agreement (AKA) protocols are proposed to authenticate legitimate users and safeguard training data. However, existing AKA protocols for client–server (C/S) architecture show security deficiencies, such as lack of user anonymity and susceptibility to password guessing attacks. In this paper, we propose a robust 2FAKA-C/S protocol based on ECC and Hash-chain technology. Our security analysis shows that the proposed protocol ensures the session keys are semantically secure and can effectively resist various attacks. The performance analysis indicates that the proposed protocol achieves a total running time of 62.644 ms and requires only 800 bits of communication overhead, showing superior computational efficiency and lower communication costs compared to existing protocols. In conclusion, the proposed protocol securely protects the training parameters in a federated learning environment and provides a reliable guarantee for data transmission.
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- 2024
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3. Reliability Prediction for New Prefabricated Track Structures Based on the Fuzzy Failure Modes, Effects, and Criticality Analysis Method
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Chao Huang, Jun Wu, Zhi Shan, Qing’e Wang, and Zhiwu Yu
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high-speed railway ,prefabricated track structure ,fuzzy FMECA ,reliability ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
This paper aims to address the problems of safety and durability in China’s ballastless track structures, particularly the lack of accurate analysis and methods for predicting the reliability of the new type of prefabricated track structure during the design phase. We propose a reliability prediction method for a new prefabricated track structure, the modular assembled track structure with built-in position retention. By adopting the fuzzy Failure Modes, Effects, and Criticality Analysis (fuzzy FMECA) method, a comprehensive assessment of fault severity, fault occurrence probability, and fault detection difficulty is conducted on the CRTS II slab track structure and the modular assembled track structure with built-in position retention. Consequently, a fault mode hazard assessment model for the new prefabricated track structure is constructed. Based on the assessment model and using a similar product method, a reliability prediction model for the new prefabricated track structure is established, and reliability prediction for the track structure is conducted. The research results indicate that the modular assembled track structure with built-in position retention has lower hazard levels and higher reliability compared to the CRTS II slab track structure. This study provides a scientific basis for the design optimization of new prefabricated track structures, helping to improve their safety and reliability, reduce operating and maintenance costs, and thereby promote the green and low-carbon development of the railway.
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- 2024
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4. Laboratory Test and Constitutive Model for Quantifying the Anisotropic Swelling Behavior of Expansive Soils
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Zhengnan Liu, Rui Zhang, Tian Lan, Yu Zhou, and Chao Huang
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road engineering ,constitutive model ,swelling test ,expansive soil ,support structure ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Expansive soils exhibit directionally dependent swelling that traditional isotropic models fail to capture. This study investigates the anisotropic swelling characteristics of expansive soil with a medium swelling potential through the use of modified oedometric testing. Vertical swelling strains can reach up to 1.71 times that of the horizontal movements, confirming intrinsic anisotropy. A nonlinear elastic constitutive model incorporates vertical and horizontal elastic moduli with respect to matric suction to characterize anisotropy. Three elastic parameters were determined through the experiments, and predictive equations were developed to estimate the unsaturated moduli. The constitutive model and predictive techniques provide practical tools to better assess expansive soil pressures considering anisotropy, offering guidelines for utilization and design. The outcomes advance understanding of these soils’ directionally dependent behavior and stress–strain–suction response.
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- 2024
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5. Game Theory-Based Interactive Control for Human–Machine Cooperative Driving
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Yangyang Zhou, Chao Huang, and Peng Hang
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autonomous vehicles ,human–machine shared control ,non-cooperative game ,model predictive control ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
To address the safety and efficient driving issues of human–machine shared control vehicles (HSCVs) in future complex traffic environments, this paper proposes a game theory-based interactive control method between HSCVs and surrounding autonomous vehicles (SVs) and involves considering different driving behaviors. In HSCV, a comprehensive driver model integrating steering control and speed control is designed based on the brain emotional learning circuit model (BELCM), and the control authority between the driver and the automation system is dynamically allocated through the evaluation of the driving safety field. Factors such as driving safety and travel efficiency that reflect personalized driving style are considered for modeling the uncertain behavior of SVs. In the interaction between HSCVs and SVs, a method based on game theory and distributed model predictive control (DMPC) that considers the uncertainty of SVs’ driving behavior is established and is finally integrated into a multi-objective constraint problem. The driver control input in an HSCV will also be introduced into the solution process. To demonstrate the feasibility of the proposed method, two test scenarios considering the driving characteristics of different SVs are established. The test results show that automation control systems can promptly stop the human driver’s dangerous operations in an HSCV and safely interact with multiple AVs with different driving characteristics.
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- 2024
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6. Diurnal Variations and Driving Factors of CO2 Flux at Water–Air Interfaces in the Open-Flow Sections of Karst Underground Rivers
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Danyang Li, Canfeng Li, Chao Huang, Hong Li, Xiongwei Xu, Xuefeng Peng, Guiren Chen, and Liankai Zhang
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open-flow section ,water–air interface ,CO2 flux ,submerged plants ,karst ,diurnal variation ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
The high-intensity partial pressure of CO2 (pCO2) in karst underground rivers rapidly releases in open-flow sections. This is an important process affecting the global karst carbon cycle. This study focuses on the diurnal variation patterns and driving factors of CO2 exchange flux at the water–air interface in the open-flow sections of typical karst underground rivers in southwestern China. The inorganic carbon in water and water–air interface exchange fluxes are observed. Three representative survey stations, i.e., the outlet of the underground river (Q1), the river sections without submerged plants (H1), and the river sections with submerged plants (H2), are selected to study the CO2 exchange process and its influencing factors. The results show that the CO2 release flux at Q1 exhibits high pressure in the daytime and low pressure in the nighttime, while H1 and H2 exhibit the opposite pattern. The photosynthesis of submerged plants significantly inhibits the carbon release flux of the river, and in the river sections where submerged plants are distributed, their biological effects have inhibited approximately 0.131 Tg C/yr of carbon emissions. This study emphasizes the significant contribution of submerged plants in restraining the release of CO2, thereby promoting carbon sequestration and storage in karst water systems.
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- 2024
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7. YUCCA2 (YUC2)-Mediated 3-Indoleacetic Acid (IAA) Biosynthesis Regulates Chloroplast RNA Editing by Relieving the Auxin Response Factor 1 (ARF1)-Dependent Inhibition of Editing Factors in Arabidopsis thaliana
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Zi-Ang Li, Yi Li, Dan Liu, David P. Molloy, Zhou-Fei Luo, Hai-Ou Li, Jing Zhao, Jing Zhou, Yi Su, Ruo-Zhong Wang, Chao Huang, and Lang-Tao Xiao
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YUC2 ,auxin ,chloroplast ,RNA editing ,ARF1 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Although recent research progress on the abundant C-to-U RNA editing events in plant chloroplasts and mitochondria has uncovered many recognition factors and their molecular mechanisms, the intrinsic regulation of RNA editing within plants remains largely unknown. This study aimed to establish a regulatory relationship in Arabidopsis between the plant hormone auxin and chloroplast RNA editing. We first analyzed auxin response elements (AuxREs) present within promoters of chloroplast editing factors reported to date. We found that each has more than one AuxRE, suggesting a potential regulatory role of auxin in their expression. Further investigation unveiled that the depletion of auxin synthesis gene YUC2 reduces the expression of several editing factors. However, in yuc2 mutants, only the expression of CRR4, DYW1, ISE2, and ECD1 editing factors and the editing efficiency of their corresponding editing sites, ndhD-2 and rps14-149, were simultaneously suppressed. In addition, exogenous IAA and the overexpression of YUC2 enhanced the expression of these editing factors and the editing efficiency at the ndhD-2 and rps14-149 sites. These results suggested a direct effect of auxin upon the editing of the ndhD-2 and rps14-149 sites through the modulation of the expression of the editing factors. We further demonstrated that ARF1, a downstream transcription factor in the auxin-signaling pathway, could directly bind to and inactivate the promoters of CRR4, DYW1, and ISE2 in a dual-luciferase reporter system, thereby inhibiting their expression. Moreover, the overexpression of ARF1 in Arabidopsis significantly reduced the expression of the three editing factors and the editing efficiency at the ndhD-2 and rps14-149 sites. These data suggest that YUC2-mediated auxin biosynthesis governs the RNA-editing process through the ARF1-dependent signal transduction pathway.
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- 2023
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8. TRIM67 Implicates in Regulating the Homeostasis and Synaptic Development of Mitral Cells in the Olfactory Bulb
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Chunyu Cai, Qihui Luo, Lanlan Jia, Yu Xia, Xinting Lan, Xiaoli Wei, Shuai Shi, Yucong Liu, Yao Wang, Zongliang Xiong, Riyi Shi, Chao Huang, and Zhengli Chen
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TRIM67 ,olfactory bulb ,development ,mitral cells ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
In recent years, olfactory dysfunction has attracted increasingly more attention as a hallmark symptom of neurodegenerative diseases (ND). Deeply understanding the molecular basis underlying the development of the olfactory bulb (OB) will provide important insights for ND studies and treatments. Now, with a genetic knockout mouse model, we show that TRIM67, a new member of the tripartite motif (TRIM) protein family, plays an important role in regulating the proliferation and development of mitral cells in the OB. TRIM67 is abundantly expressed in the mitral cell layer of the OB. The genetic deletion of TRIM67 in mice leads to excessive proliferation of mitral cells in the OB and defects in its synaptic development, resulting in reduced olfactory function in mice. Finally, we show that TRIM67 may achieve its effect on mitral cells by regulating the Semaphorin 7A/Plexin C1 (Sema7A/PlxnC1) signaling pathway.
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- 2023
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9. Conversion of CO2 Hydrogenation to Methanol over K/Ni Promoted MoS2/MgO Catalyst
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Siyi Jiang, Yujing Weng, Yangbin Ren, Shihang Meng, Xiaoman Li, Chao Huang, Yulong Zhang, and Qi Sun
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CO2 hydrogenation ,molybdenum disulfide ,methanol ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
The chemical transformation of carbon dioxide (CO2) not only reduces the amount of carbon dioxide emitted into the Earth’s atmosphere by humans, but also produces carbon compounds that can be used as precursors for chemical and fuel production. Herein, a selective catalytic conversion of carbon dioxide to methanol is achieved by a bifunctional molybdenum disulfide catalyst (MoS2) with magnesium oxide and nickel and potassium promoters. Molybdenum disulfide prepared by the supercritical ethanol method has a large specific surface area and presents good catalytic performance with high methanol selectivity when loaded with potassium (K) and nickel (Ni) promoters. In addition, the catalysts were evaluated and it was founded that the addition of the K-promoter improved methanol selectivity. This research provides a new strategy for improved product selectivity and space–time yield (STY) of methanol in CO2 hydrogenation.
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- 2023
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10. Complexation and Separation of Trivalent Actinides and Lanthanides by a Novel DGA Derived from Macrocyclic Crown Ether: Synthesis, Extraction, and Spectroscopic and Density Functional Theory Studies
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Yu Fan, Youzhen Li, Xi Shu, Rulei Wu, Shanyong Chen, Yongdong Jin, Chao Xu, Jing Chen, Chao Huang, and Chuanqin Xia
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Chemistry ,QD1-999 - Published
- 2021
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11. Rapid Detection of IgM Antibodies against the SARS-CoV‑2 Virus via Colloidal Gold Nanoparticle-Based Lateral-Flow Assay
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Chao Huang, Tian Wen, Feng-Juan Shi, Xiao-Yan Zeng, and Yong-Jun Jiao
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Chemistry ,QD1-999 - Published
- 2020
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12. The Effect of the Histone Chaperones HSPA8 and DEK on Tumor Immunity in Hepatocellular Carcinoma
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Chuanxin Yang, Yaodi Shao, Xiangjun Wang, Jie Wang, Puxiongzhi Wang, Chao Huang, Wei Wang, and Jian Wang
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hepatocellular carcinoma ,histone chaperone ,HSPA8 ,DEK ,tumor immunity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Complex immune contexture leads to resistance to immunotherapy in hepatocellular carcinoma (HCC), and the need for new potential biomarkers of immunotherapy in HCC is urgent. Histone chaperones are vital determinants of gene expression and genome stability that regulate tumor development. This study aimed to investigate the effect of histone chaperones on tumor immunity in HCC. Bioinformatics analyses were initially performed using The Cancer Genome Atlas (TCGA) database, and were validated using the Gene Expression Omnibus (GEO) database and the International Cancer Genome Consortium (ICGC) database. Immune-related histone chaperones were screened with the Spearman rank coefficient. Consensus clustering was utilized to divide the HCC samples into two clusters. ESTIMATE, CIBERSORT and ssGSEA analyses were performed to assess immune infiltration. The expression of immunomodulatory genes, chemokines and chemokine receptors was analyzed to evaluate sensitivity to immunotherapy. The differentially expressed genes (DEGs) were included in weighted gene coexpression network analysis (WGCNA) to identify the hub genes. Enrichment analyses were used to investigate the functions of the hub genes. The Kaplan-Meier method and log-rank test were conducted to draw survival curves. A Cox regression analysis was utilized to identify independent risk factors affecting prognosis. HSPA8 and DEK were screened out from 36 known histone chaperones based on their strongest correlation with the ESTIMATE score. Cluster 2, with high HSPA8 expression and low DEK expression, tended to have stronger immune infiltration and better sensitivity to immunotherapy than Cluster 1, with low HSPA8 expression and high DEK expression. Furthermore, WGCNA identified 12 hub genes closely correlated with immune infiltration from the DEGs of the two clusters, of which FBLN2 was proven to be an independent protective factor of HCC patients. HSPA8 and DEK are expected to be biomarkers for precisely predicting the effect of immunotherapy, and FBLN2 is expected to be a therapeutic target of HCC.
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- 2023
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13. A Novel Filter-Level Deep Convolutional Neural Network Pruning Method Based on Deep Reinforcement Learning
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Yihao Feng, Chao Huang, Long Wang, Xiong Luo, and Qingwen Li
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deep convolutional neural network ,filter pruning ,deep deterministic policy gradient ,Taylor expansion ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Deep neural networks (DNNs) have achieved great success in the field of computer vision. The high requirements for memory and storage by DNNs make it difficult to apply them to mobile or embedded devices. Therefore, compression and structure optimization of deep neural networks have become a hot research topic. To eliminate redundant structures in deep convolutional neural networks (DCNNs), we propose an efficient filter pruning framework via deep reinforcement learning (DRL). The proposed framework is based on a deep deterministic policy gradient (DDPG) algorithm for filter pruning rate optimization. The main features of the proposed framework are as follows: (1) AA tailored reward function considering both accuracy and complexity of DCNN is proposed for the training of DDPG and (2) a novel filter sorting criterion based on Taylor expansion is developed for filter pruning selection. To illustrate the effectiveness of the proposed framework, extensive comparative studies on large public datasets and well-recognized DCNNs are conducted. The experimental results demonstrate that the Taylor-expansion-based filter sorting criterion is much better than the widely used minimum-weight-based criterion. More importantly, the proposed filter pruning framework can achieve over 10× parameter compression and 3× floating point operations (FLOPs) reduction while maintaining similar accuracy to the original network. The performance of the proposed framework is promising compared with state-of-the-art DRL-based filter pruning methods.
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- 2022
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14. Lysyl Oxidase Family Proteins: Prospective Therapeutic Targets in Cancer
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Wei Wang, Xiangjun Wang, Feng Yao, and Chao Huang
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lysyl oxidase (LOX) ,extracellular matrix (ECM) ,cancer ,LOX family ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The lysyl oxidase (LOX) family, consisting of LOX and LOX-like proteins 1–4 (LOXL1–4), is responsible for the covalent crosslinking of collagen and elastin, thus maintaining the stability of the extracellular matrix (ECM) and functioning in maintaining connective tissue function, embryonic development, and wound healing. Recent studies have found the aberrant expression or activity of the LOX family occurs in various types of cancer. It has been proved that the LOX family mainly performs tumor microenvironment (TME) remodeling function and is extensively involved in tumor invasion and metastasis, immunomodulation, proliferation, apoptosis, etc. With relevant translational research in progress, the LOX family is expected to be an effective target for tumor therapy. Here, we review the research progress of the LOX family in tumor progression and therapy to provide novel insights for future exploration of relevant tumor mechanism and new therapeutic targets.
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- 2022
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15. SOX2 Promotes Invasion in Human Bladder Cancers through MMP2 Upregulation and FOXO1 Downregulation
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Qipeng Xie, Xiaohui Hua, Chao Huang, Xin Liao, Zhongxian Tian, Jiheng Xu, Yunping Zhao, Guosong Jiang, Haishan Huang, and Chuanshu Huang
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SOX2 ,SKP2 ,FOXO1 ,nucleolin ,bladder cancer invasion ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
SOX2, a member of the SRY-related HMG-box (SOX) family, is abnormally expressed in many tumors and associated with cancer stem cell-like properties. Previous reports have shown that SOX2 is a biomarker for cancer stem cells in human bladder cancer (BC), and our most recent study has indicated that the inhibition of SOX2 by anticancer compound ChlA-F attenuates human BC cell invasion. We now investigated the mechanisms through which SOX2 promotes the invasive ability of BC cells. Our studies revealed that SOX2 promoted SKP2 transcription and increased SKP2-accelerated Sp1 protein degradation. As Sp1 is a transcriptionally regulated gene, HUR transcription was thereby attenuated, and, in the absence of HUR, FOXO1 mRNA was degraded fast, which promoted BC cell invasion. In addition, SOX2 promoted BC invasion through the upregulation of nucleolin transcription, which resulted in increased MMP2 mRNA stability and expression. Collectively, our findings show that SOX2 promotes BC invasion through both SKP2-Sp1-HUR-FOXO1 and nucleolin-MMP2 dual axes.
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- 2022
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16. Polysaccharide from Salviae miltiorrhizae Radix et Rhizoma Attenuates the Progress of Obesity-Induced Non-Alcoholic Fatty Liver Disease through Modulating Intestinal Microbiota-Related Gut–Liver Axis
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Lixia Li, Xinting Lan, Xi Peng, Shuai Shi, Yanlin Zhao, Wentao Liu, Qihui Luo, Lanlan Jia, Bin Feng, Zhengli Chen, Yuanfeng Zou, and Chao Huang
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Salviae miltiorrhizae Radix et Rhizoma ,polysaccharide ,obesity ,NAFLD ,gut–liver axis ,gut microbiota ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Non-alcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disease worldwide, thus treatments for it have attracted lots of interest. In this study, the Salviae miltiorrhizae Radix et Rhizoma (SMRR) polysaccharide was isolated by hot water extraction and ethanol precipitation, and then purified by DEAE anion exchange chromatography and gel filtration. With a high-fat-diet-induced obesity/NAFLD mouse model, we found that consumption of the SMRR polysaccharide could remarkably reverse obesity and its related progress of NAFLD, including attenuated hepatocellular steatosis, hepatic fibrosis and inflammation. In addition, we also reveal the potential mechanism behind these is that the SMRR polysaccharide could regulate the gut–liver axis by modulating the homeostasis of gut microbiota and thereby improving intestinal function.
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- 2022
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17. Polyphyllin I Promotes Autophagic Cell Death and Apoptosis of Colon Cancer Cells via the ROS-Inhibited AKT/mTOR Pathway
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Qihui Luo, Lanlan Jia, Chao Huang, Qi Qi, Asad Jahangir, Yu Xia, Wentao Liu, Riyi Shi, Li Tang, and Zhengli Chen
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polyphyllin I ,ROS ,AKT/mTOR ,autophagy ,SW480 cells ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Colon cancer is a common malignant tumor of the digestive tract, and it is considered among the biggest killers. Scientific and reasonable treatments can effectively improve the survival rate of patients if performed in the early stages. Polyphyllin I (PPI), a pennogenyl saponin isolated from Paris polyphylla var. yunnanensis, has exhibited strong anti-cancer activities in previous studies. Here, we report that PPI exhibits a cytotoxic effect on colon cancer cells. PPI suppressed cell viability and induced autophagic cell death in SW480 cells after 12 and 24 h, with the IC50 values 4.9 ± 0.1 μmol/L and 3.5 ± 0.2 μmol/L, respectively. Furthermore, we found PPI induced time-concentration-dependent autophagy and apoptosis in SW480 cells. In addition, down-regulated AKT/mTOR activity was found in PPI-treated SW480 cells. Increased levels of ROS might link to autophagy and apoptosis because reducing the level of ROS by antioxidant N-acetylcysteine (NAC) treatment mitigated PPI-induced autophagy and apoptosis. Although we did not know the molecular mechanism of how PPI induced ROS production, this is the first study to show that PPI induces ROS production and down-regulates the AKT/mTOR pathway, which subsequently promotes the autophagic cell death and apoptosis of colon cancer cells. This present study reports PPI as a potential therapeutic agent for colon cancer and reveals its underlying mechanisms of action.
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- 2022
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18. TRIM67 Deficiency Exacerbates Hypothalamic Inflammation and Fat Accumulation in Obese Mice
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Lanlan Jia, Zhengli Chen, Ting Pan, Yu Xia, Junbo He, Asad Jahangir, Xiaoli Wei, Wentao Liu, Riyi Shi, Chao Huang, and Qihui Luo
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TRIM67 ,hypothalamus ,obesity ,energy homeostasis ,neuroinflammation ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Obesity has achieved the appearance of a global epidemic and is a serious cause for concern. The hypothalamus, as the central regulator of energy homeostasis, plays a critical role in regulating food intake and energy expenditure. In this study, we show that TRIM67 in the hypothalamus was responsive to body-energy homeostasis whilst a deficiency of TRIM67 exacerbated metabolic disorders in high-fat-diet-induced obese mice. We found exacerbated neuroinflammation and apoptosis in the hypothalamus of obese TRIM67 KO mice. We also found reduced BDNF in the hypothalamus, which affected the fat sympathetic nervous system innervation and contributed to lipid accumulation in adipose tissue under high-fat-diet exposure. In this study, we reveal potential implications between TRIM67 and the hypothalamic function responding to energy overuptake as well as a consideration for the therapeutic diagnosis of obesity.
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- 2022
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19. Ameliorating Effects of TRIM67 against Intestinal Inflammation and Barrier Dysfunction Induced by High Fat Diet in Obese Mice
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Qihui Luo, Asad Jahangir, Junbo He, Chao Huang, Yu Xia, Lanlan Jia, Xiaoli Wei, Ting Pan, Yanni Du, Bin Mu, Huan Gong, Wentao Liu, Saif Ur-Rehman, Kangcheng Pan, and Zhengli Chen
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high fat diet ,TRIM67 ,knockout ,intestine ,inflammation ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Tripartite Motif 67 (TRIM67) is an important member of TRIM family proteins, which participates in different cellular processes including immune response, proliferation, differentiation, carcinogenesis, and apoptosis. In recent years, a high fat diet (HFD) has remained one of the main causes of different metabolic diseases and increases in intestinal permeability as well as inducing intestinal inflammation. The current study investigated the protective effects of TRIM67 in the ileum and colon of obese mice. 4-week-old wild-type (WT) C57BL/6N mice and TRIM67 knockout (KO) C57BL/6N mice were selected and randomly divided into four sub-groups, which were fed with control diet (CTR) or HFD for 14 weeks. Samples were collected at the age of 18 weeks for analysis. To construct an in vitro obesity model, over-expressed IPEC-J2 cells (porcine intestinal cells) with Myc-TRIM67 were stimulated with palmitic acid (PA), and its effects on the expression level of TRM67, inflammatory cytokines, and barrier function were evaluated. The KO mice showed pathological lesions in the ileum and colon and this effect was more obvious in KO mice fed with HFD. In addition, KO mice fed with a HFD or CTR diet had increased intestinal inflammation, intestinal permeability, and oxidative stress compared to that WT mice fed with these diets, respectively. Moreover, IPEC-J2 cells were transfected with TRIM67 plasmid to perform the same experiments after stimulation with PA, and the results were found consistent with the in vivo evaluations. Taken together, our study proved for the first time that HFD and TRIM67 KO mice have synergistic damaging effects on the intestine, while TRIM67 plays an important protective role in HFD-induced intestinal damage.
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- 2022
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20. Chinese Named Entity Recognition of Geological News Based on BERT Model
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Chao Huang, Yuzhu Wang, Yuqing Yu, Yujia Hao, Yuebin Liu, and Xiujian Zhao
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BERT ,named entity recognition ,geological news ,CRF ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
With the ongoing progress of geological survey work and the continuous accumulation of geological data, extracting accurate information from massive geological data has become increasingly difficult. To fully mine and utilize geological data, this study proposes a geological news named entity recognition (GNNER) method based on the bidirectional encoder representations from transformers (BERT) pre-trained language model. This solves the problems of traditional word vectors that are difficult to represent context semantics and the single extraction effect and can also help construct the knowledge graphs of geological news. First, the method uses the BERT pre-training model to embed words in the geological news text, and the dynamically obtained word vector is used as the model’s input. Second, the word vector is sent to a bidirectional long short-term memory model for further training to obtain contextual features. Finally, the corresponding six entity types are extracted using conditional random field sequence decoding. Through experiments on the constructed Chinese geological news dataset, the average F1 score identified by the model is 0.839. The experimental results show that the model can better identify news entities in geological news.
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- 2022
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21. Loss of TRIM67 Attenuates the Progress of Obesity-Induced Non-Alcoholic Fatty Liver Disease
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Chao Huang, Xiaoli Wei, Qihui Luo, Yu Xia, Ting Pan, Junbo He, Asad Jahangir, Lanlan Jia, Wentao Liu, Yuanfeng Zou, Lixia Li, Hongrui Guo, Yi Geng, and Zhengli Chen
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TRIM67 ,obesity ,NAFLD ,inflammation ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Obesity is considered as a major cause for the development and progress of non-alcoholic fatty liver disease (NAFLD), which is one of the most prevalent chronic liver diseases worldwide. However, molecular mechanisms that implicate in obesity-driven pathophysiology of NAFLD are not well defined. Here, we report a tripartite motif (TRIM) protein family member—TRIM67—that is hardly expressed in liver but is inducible on obese conditions. Enhanced expression of TRIM67 activates hepatic inflammation to disturb lipid metabolic homeostasis and promote the progress of NAFLD induced by obesity, while the deficiency in TRIM67 is protective against these pathophysiological processes. Finally, we show that the important transcription coactivator PGC-1α implicates in the response of hepatic TRIM67 to obesity.
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- 2022
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22. Natural Clinopyroxene Reference Materials for in situ Sr Isotopic Analysis via LA-MC-ICP-MS
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Han Zhao, Xin-Miao Zhao, P. J. Le Roux, Wen Zhang, Hao Wang, Lie-Wen Xie, Chao Huang, Shi-Tou Wu, Jin-Hui Yang, Fu-Yuan Wu, and Yue-Heng Yang
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clinopyroxene ,in situ ,87Sr/86Sr analysis ,LA-MC-ICP-MS ,natural reference material ,Chemistry ,QD1-999 - Abstract
Clinopyroxene is a major host mineral for lithophile elements in the mantle lithosphere, and therefore, its origin is vital for constraints on mantle evolution and melt generation. In situ Sr isotopic measurement of clinopyroxene has been available since the recent development of laser ablation multicollector inductively coupled plasma mass spectrometry (LA-MC-ICP-MS) in the 2000s. Therefore, there is an increasing demand for natural clinopyroxene reference materials for Sr isotope microanalysis. In this contribution, we present six natural clinopyroxene reference materials from South Africa (JJG1424) and China (YY09-47, YY09-04, YY09-24, YY12-01, and YY12-02) for Sr isotope microanalysis. The Sr content of these clinopyroxenes ranges from 50 to 340 μg g−1, which covers most natural clinopyroxene compositions. Homogeneity of these potential reference materials were investigated and evaluated in detail over a 2-year period using 193-nm nanosecond and 257-nm femtosecond laser systems coupled to either a Neptune or Neptune Plus MC-ICP-MS. Additionally, the major and trace element of these clinopyroxenes were examined by electron probe microanalyzer (EPMA) as well as solution and laser ICP-MS. The in situ87Sr/86Sr values obtained for the six natural clinopyroxene reference materials agree well with data obtained using the thermal ionization mass spectrometer (TIMS) method. The Sr isotopic stability and homogeneity of these clinopyroxenes make them potential reference materials for in situ Sr microanalysis to correct instrumental fractionation or as quality control materials for analytical sessions. The new Sr isotope data provided here might be beneficial for microbeam analysis in the geochemical community.
- Published
- 2020
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- View/download PDF
23. BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis
- Author
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Chao Huang, Huan Gong, Bin Mu, Xinting Lan, Chengcheng Yang, Jinlong Tan, Wentao Liu, Yuanfeng Zou, Lixia Li, Bin Feng, Xia He, Qihui Luo, and Zhengli Chen
- Subjects
BAF-L ,spermiogenesis ,histones ,protamines ,male infertility ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Maturing male germ cells undergo a unique developmental process in spermiogenesis that replaces nucleosomal histones with protamines, the process of which is critical for testicular development and male fertility. The progress of this exchange is regulated by complex mechanisms that are not well understood. Now, with mouse genetic models, we show that barrier-to-autointegration factor-like protein (BAF-L) plays an important role in spermiogenesis and spermatozoal function. BAF-L is a male germ cell marker, whose expression is highly associated with the maturation of male germ cells. The genetic deletion of BAF-L in mice impairs the progress of spermiogenesis and thus male fertility. This effect on male fertility is a consequence of the disturbed homeostasis of histones and protamines in maturing male germ cells, in which the interactions between BAF-L and histones/protamines are implicated. Finally, we show that reduced testicular expression of BAF-L represents a risk factor of human male infertility.
- Published
- 2022
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24. Preparation of a Sensor Based on Biomass Porous Carbon/Covalent-Organic Frame Composites for Pesticide Residues Detection
- Author
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Yali Liu, Mingyue Zhou, Chen Jin, Jinxiang Zeng, Chao Huang, Qiuye Song, and Yonggui Song
- Subjects
sensor ,AChE ,integrated electrode ,biomass carbon materials ,detecting ,Chemistry ,QD1-999 - Abstract
In this work, a covalent-organic framework with high carbon and nitrogen content microstructures (named COF-LZU1), assisted by 3D nitrogen-containing kenaf stem composites (represented as COF-LZU1/3D-KSCs), was constructed. Moreover, it was utilized for immobilizing acetylcholinesterase (AChE) for identifying trichlorfon, a commonly applied organophosphorus (OP) pesticide. The development of COF-LZU1/3D-KSC was affirmed by SEM, PXRD, and EDXS. The findings confirmed that COF-LZU1 microstructures were uniformly developed on 3D-KSC holes using a one-step synthesis approach, which can substantially enhance the effective surface area. Also, the COF-LZU1/3D-KSC composite contains not only the nitrogen element in COF-LZU1 but also the nitrogen element in 3D-KSC, which will greatly improve the biocompatibility of the material. The AChE/COF-LZU1/3D-KSC integrated electrode was fabricated by directly fixing a large amount of AChE on the composite. At the same time, the integrated electrode had good detection efficiency for trichlorfon. Improved stabilization, a wide-linear-range (0.2–19 ng/mL), and a lower detection limit (0.067 ng/mL) have been displayed by the sensor. Therefore, this sensor can be used as an important platform for the on-site detection of OP residue.
- Published
- 2020
- Full Text
- View/download PDF
25. Three-Component One-Pot Approach to Highly Efficient and Sustainable Synthesis of the Functionalized Quinolones via Linear/Branched Domino Protocols, Key Synthetic Methods for the Floxacin of Quinolone Drugs
- Author
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Hairui Bai, Fujun Liu, Xiaojing Wang, Ping Wang, and Chao Huang
- Subjects
Chemistry ,QD1-999 - Published
- 2018
- Full Text
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26. Mitochondrial Ceramide Effects on the Retinal Pigment Epithelium in Diabetes
- Author
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Yan Levitsky, Sandra S. Hammer, Kiera P. Fisher, Chao Huang, Travan L. Gentles, David J. Pegouske, Caimin Xi, Todd A. Lydic, Julia V. Busik, and Denis A. Proshlyakov
- Subjects
diabetes ,retinopathy ,acid sphingomyelinase ,mitochondria ,ceramide ,sphingolipid ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Mitochondrial damage in the cells comprising inner (retinal endothelial cells) and outer (retinal pigment epithelium (RPE)) blood–retinal barriers (BRB) is known to precede the initial BRB breakdown and further histopathological abnormalities in diabetic retinopathy (DR). We previously demonstrated that activation of acid sphingomyelinase (ASM) is an important early event in the pathogenesis of DR, and recent studies have demonstrated that there is an intricate connection between ceramide and mitochondrial function. This study aimed to determine the role of ASM-dependent mitochondrial ceramide accumulation in diabetes-induced RPE cell damage. Mitochondria isolated from streptozotocin (STZ)-induced diabetic rat retinas (7 weeks duration) showed a 1.64 ± 0.29-fold increase in the ceramide-to-sphingomyelin ratio compared to controls. Conversely, the ceramide-to-sphingomyelin ratio was decreased in the mitochondria isolated from ASM-knockout mouse retinas compared to wild-type littermates, confirming the role of ASM in mitochondrial ceramide production. Cellular ceramide was elevated 2.67 ± 1.07-fold in RPE cells derived from diabetic donors compared to control donors, and these changes correlated with increased gene expression of IL-1β, IL-6, and ASM. Treatment of RPE cells derived from control donors with high glucose resulted in elevated ASM, vascular endothelial growth factor (VEGF), and intercellular adhesion molecule 1 (ICAM-1) mRNA. RPE from diabetic donors showed fragmented mitochondria and a 2.68 ± 0.66-fold decreased respiratory control ratio (RCR). Treatment of immortalized cell in vision research (ARPE-19) cells with high glucose resulted in a 25% ± 1.6% decrease in citrate synthase activity at 72 h. Inhibition of ASM with desipramine (15 μM, 1 h daily) abolished the decreases in metabolic functional parameters. Our results are consistent with diabetes-induced increase in mitochondrial ceramide through an ASM-dependent pathway leading to impaired mitochondrial function in the RPE cells of the retina.
- Published
- 2020
- Full Text
- View/download PDF
27. Extracellular Vesicle-Induced Classical Complement Activation Leads to Retinal Endothelial Cell Damage via MAC Deposition
- Author
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Chao Huang, Kiera P. Fisher, Sandra S. Hammer, and Julia V. Busik
- Subjects
diabetic retinopathy ,extracellular vesicles ,complement system ,retinal vascular damage ,human retinal endothelial cells (hrecs) ,membrane attack complex (mac) ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Several studies have suggested that there is a link between membrane attack complex (MAC) deposition in the retina and the progression of diabetic retinopathy (DR). Our recent investigation demonstrated that circulating IgG-laden extracellular vesicles contribute to an increase in retinal vascular permeability in DR through activation of the complement system. However, the mechanism through which extracellular vesicle-induced complement activation contributes to retinal vascular cytolytic damage in DR is not well understood. In this study, we demonstrate that IgG-laden extracellular vesicles in rat plasma activate the classical complement pathway, and in vitro Streptozotocin (STZ)-induced rat diabetic plasma results in MAC deposition and cytolytic damage in human retinal endothelial cells (HRECs). Moreover, removal of the plasma extracellular vesicles reduced the MAC deposition and abrogated cytolytic damage seen in HRECs. Together, the results of this study demonstrate that complement activation by IgG-laden extracellular vesicles in plasma could lead to MAC deposition and contribute to endothelium damage and progression of DR.
- Published
- 2020
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28. Mechanical Behavior of Circular Steel Tubular Beam-Columns Corroded Uniformly in Atmospheric Environment
- Author
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Zhaoqi Wu, Yuan Wei, Xintao Wang, Chao Huang, and Shao-Fei Jiang
- Subjects
atmospheric environment ,circular steel tube ,uniform corrosion ,experimental research ,load carrying capacity prediction ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Circular steel tube members with the absence of anticorrosive protection or coating failure are prone to uniform corrosion, which threatens the reliability and safety of members in the atmospheric environment. To fully study the mechanical behavior of uniformly corroded circular steel tubes, compression test and theoretical analysis were conducted, and two methods considering section reduction and material degradation, respectively, were adopted for the calculation of ultimate load carrying capacities of specimens. The results indicate that uniform corrosion did not change the failure modes of specimens, and all of them belonged to global buckling failure. The load carrying capacities and stiffness of specimens decreased with the increase of corrosion ratio, and the degree of reduction was greater than that of material degradation, showing a linear relationship with the corrosion rate. Under the same corrosion ratio, the specimens with larger eccentricity represented more obvious load carrying capacity and stiffness degradation. The load carrying capacities predicted by both methods were in good agreement with the test results and had a certain safety margin. The conservative degree of calculation results from three specifications followed a descending order of ANSI/AISC 360-16, GB 50017-2017, and EN 1993-1-1. Under the same corrosion ratio, the load carrying capacity variation of specimens between one-sided corrosion and two-sided corrosion was less than 3%.
- Published
- 2020
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29. An Efficient Strategy for the Fabrication of CuS as a Highly Excellent and Recyclable Photocatalyst for the Degradation of Organic Dyes
- Author
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Na Qin, Wutao Wei, Chao Huang, and Liwei Mi
- Subjects
cus ,photocatalysis ,simulated solar light ,organic dyes ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
An effective and practical in situ sulfuration approach has been developed in this work, for the fabrication of CuS with a 3D hierarchical network structure under mild preparation conditions. The prepared CuS consists of a primary structure of the multi-structure interchange copper foam precursor, and a secondary structure of nanoplates. The structural characteristics, morphologies, and photocatalytic performances of the prepared photocatalyst were investigated systematically. To evaluate the photocatalytic performance of the prepared CuS samples, we investigated the degradation of MB (methylene blue), RhB (Rhodamine B), and MB/RhB dye solutions over the samples under the irradiation of simulated solar light. Specifically, the degradation of RhB rapidly reached ≈100.0% after simulated solar light irradiation for 25 min, which is higher than those of P25 (83.0%) and bulk CuS (54.8%). For the mixed systems of MB/RhB, both the degradations of MB and RhB reached up to ≈99.0% after simulated solar light irradiation for 25 min. The superior photocatalytic performances of the prepared samples are attributed to the synergistic effects of high optical absorption, large specific surface area, and abundant active sites. The prepared catalysts can retain the photocatalytic activities during the entire reaction process without significant loss after four catalytic cycles, which reveals that the CuS with a stable 3D hierarchical network structure has a promising prospect as an ideal recyclable catalyst.
- Published
- 2019
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30. Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana
- Author
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Wei Hu, Xiaowan Hou, Chao Huang, Yan Yan, Weiwei Tie, Zehong Ding, Yunxie Wei, Juhua Liu, Hongxia Miao, Zhiwei Lu, Meiying Li, Biyu Xu, and Zhiqiang Jin
- Subjects
Aquaporin (AQP) ,abiotic stress ,banana ,development ,expression analysis ,fruit postharvest ripening ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.
- Published
- 2015
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31. The Effect of Different Mixed Organic Solvents on the Properties of p(OPal-MMA) Gel Electrolyte Membrane for Lithium Ion Batteries
- Author
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Lanlan Tian, Mengkun Wang, Lian Xiong, Haijun Guo, Chao Huang, Hairong Zhang, and Xinde Chen
- Subjects
lithium ion battery ,phase inversion ,mixed solvent ,gel polymer electrolyte ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
A solvent is a key factor during polymer membrane preparation, and it is directly related to application performance as a separator for lithium ion battery (LIB). In this study, different mixed solvents were employed to prepare polymer (p(OPal-MMA)) membranes by the phase inversion technique. The polymer membrane then absorbed liquid electrolytes to obtain gel electrolytes (GPEs). The surface morphologies and porosities of these membranes were investigated, and lithium ion transferences and electrochemical performances of these GPEs were also measured. The membrane displayed an interconnected three-dimensional framework structure with uniformly distributed pores when using DMF as a porogen. When combined with acetone as the component solvent, the prepared GPE displayed the largest lithium ion transference number (0.706), the highest porosity (42.6%) and ion conductivity (3.99 × 10−3 S/cm). Even when assembled as Li/GPE/LiFePO4 cell, it exhibited the highest initial specific capacity of 167 mAh/g and retained most capacity (162 mAh/g) after 50 cycles. The results presented here probably provide reference for choosing an appropriate mixed solvent in fabricating polymer membranes.
- Published
- 2018
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- View/download PDF
32. A Novel Chemometric Method for the Prediction of Human Oral Bioavailability
- Author
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Jinyou Duan, Yang Ling, Yonghua Wang, Hua Yu, Chao Huang, Yan Li, Wuxia Zhang, and Xue Xu
- Subjects
oral bioavailability ,quantitative structure activity relationship ,cytochrome P4503A4 and P4502D6 ,P-glycoprotein ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Orally administered drugs must overcome several barriers before reaching their target site. Such barriers depend largely upon specific membrane transport systems and intracellular drug-metabolizing enzymes. For the first time, the P-glycoprotein (P-gp) and cytochrome P450s, the main line of defense by limiting the oral bioavailability (OB) of drugs, were brought into construction of QSAR modeling for human OB based on 805 structurally diverse drug and drug-like molecules. The linear (multiple linear regression: MLR, and partial least squares regression: PLS) and nonlinear (support-vector machine regression: SVR) methods are used to construct the models with their predictivity verified with five-fold cross-validation and independent external tests. The performance of SVR is slightly better than that of MLR and PLS, as indicated by its determination coefficient (R2) of 0.80 and standard error of estimate (SEE) of 0.31 for test sets. For the MLR and PLS, they are relatively weak, showing prediction abilities of 0.60 and 0.64 for the training set with SEE of 0.40 and 0.31, respectively. Our study indicates that the MLR, PLS and SVR-based in silico models have good potential in facilitating the prediction of oral bioavailability and can be applied in future drug design.
- Published
- 2012
- Full Text
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33. A Link Between Chemical Structure and Biological Activity in Triterpenoids
- Author
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Cunqin Wang, Lei Wang, Hao Xu, Li Zang, Rongbin Wang, Hongting Wang, Chao Huang, and Ying Chen
- Subjects
Cancer Research ,Molecular Structure ,Chemistry ,Chemical structure ,Antineoplastic Agents ,Apoptosis ,Biological activity ,General Medicine ,Computational biology ,Triterpenes ,Patents as Topic ,Triterpenoid ,Oncology ,Pentacyclic triterpenoid ,Neoplasms ,Drug Discovery ,Cancer cell ,Humans ,Pharmacology (medical) - Abstract
Background: Plants that are rich in triterpenoid compounds possess various biological activities and are reported in many scientific works. Triterpenoids, because of their broad sources, wide variety, high medicinal value, and anti-tumor properties, have drawn great attention from scientists. However, the lack of approach to understand the link between their chemical structures and biological activities has limited the fundamental comprehension of these compounds in cancer therapy. Objective: The aim of the study is to summarize the list of plants with triterpenoids and their derivatives that are a source of potential novel therapeutic anti-cancer agents by interpreting the network of anti-cancer activity and the structures of triterpenoids and their derivatives. Methods: This work focuses on analyzing relevant patents and references that detail the structure of triterpenoids and their derivatives for the treatment of tumors. Result: Pentacyclic triterpenoid plays a more important role in improving the autophagic signaling pathways of cancer cells compared to tetracyclic triterpenoid. Conclusion: The heterogenous skeleton structure of triterpenoids impairs programmed cell death signaling pathways in various cancers.
- Published
- 2022
34. Q[8]/SC[6]A-based framework constructed via OSIQ for metal ion capture
- Author
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Chao Huang, Jiang-Lin Zhao, Zhu Tao, Li-Xia Chen, Qian-Jiang Zhu, Kai Chen, Ming Liu, and Li-Fei Tian
- Subjects
Alkaline earth metal ,Materials science ,Seawater desalination ,Supramolecular chemistry ,chemistry.chemical_element ,General Chemistry ,Alkali metal ,Water softening ,Ion ,Metal ,chemistry ,visual_art ,Caesium ,visual_art.visual_art_medium ,Physical chemistry - Abstract
Since the outer surface interaction of Q[n]s (OSIQ, including self-, anion- and aromatic-induced OSIQs) was proposed in 2014, it has become the most important research area in our group to construct various Q[n]-based supramolecular frameworks via the OSIQ strategy. Herein, we report a novel supramolecular framework constructed using cucurbit[8]uril (Q[8]) and 4-sulfocalix[6]arene (SC[6]A). This Q[8]/SC[6]A-based supramolecular framework is a product via the perfect combination of self-, anion- and aromatic-induced OSIQs. This framework has the characteristics of easy preparation and high stability with the most important feature being the sequence selective capture of specific metal cations, such as common alkali- and alkaline earth metal ions, and renewability. Thus, this framework may be used in seawater desalination, potassium ion enrichment, radioactive cesium ion pollution source treatment, Gruinard's treatment or water softening and other applications.
- Published
- 2022
35. FOXM1-mediated activation of phospholipase D1 promotes lipid droplet accumulation and reduces ROS to support paclitaxel resistance in metastatic cancer cells
- Author
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Xiaoping Chen, Huifang Liang, Bixiang Zhang, Wei Zhang, Jianping Zhao, Sanshan Jin, Chao Huang, Xin Zhang, and Yue Yuan
- Subjects
Paclitaxel ,Endoplasmic reticulum ,Forkhead Box Protein M1 ,Cancer ,Lipid Droplets ,medicine.disease ,Biochemistry ,Metastasis ,Mice ,chemistry.chemical_compound ,chemistry ,Drug Resistance, Neoplasm ,In vivo ,Cell Line, Tumor ,Neoplasms ,Physiology (medical) ,Lipid droplet ,Cancer cell ,Phospholipase D ,Cancer research ,medicine ,Animals ,Reactive Oxygen Species ,Phospholipase D1 - Abstract
Chemoresistance is a major challenge for the treatment of cancer with metastasis. We investigated the mechanisms of lipid metabolites involved in drug resistance. Here, metastatic cancer cells isolated from mouse models were resistant to paclitaxel treatment in vitro and in vivo when compared with parental cancer cells. FOXM1, an oncogenic transcriptional factor, was highly expressed in metastatic cancer cells, and overexpression of FOXM1 conferred parental cancer cells resistance to paclitaxel. Lipidomic analysis showed that FOXM1 increased unsaturated triglyceride (TG) and phosphatidylcholine (PC) abundance, which are the main components of lipid droplet (LD). Inhibition of LD formation sensitized cells to paclitaxel. Mechanistically, the enzyme phospholipase D1 (PLD1) was identified as a potential effector target of FOXM1. PLD1 promoted LD accumulation, which reduced the level of reactive oxygen species (ROS) and maintained endoplasmic reticulum (ER) homeostasis in resistant cells with the treatment of paclitaxel. Moreover, inhibition of PLD1 reversed FOXM1-conferred paclitaxel resistance in vitro and in vivo. This study, for the first time, reveals the role of FOXM1-mediated PLD1 in LD accumulation and paclitaxel resistance. Targeting PLD1 or LD formation may help reverse chemoresistance in metastatic cancer cells. Generally, our results identified FOXM1 as a driver of paclitaxel resistance via activation of PLD1 to promote of LD accumulation, which contributes to the maintenace of ER homeostasis when metastatic cancer cells are confronted with ROS induced by paclitaxel.
- Published
- 2022
36. A facile method to enhance the output performance of triboelectric nanogenerators based on coordination polymers by modulating terminal coordination groups
- Author
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Chao Huang, Yingying Zhang, Sheng Zhang, Wenjie Wang, and Liwei Mi
- Subjects
chemistry.chemical_classification ,Materials science ,Polarity (physics) ,General Chemistry ,Polymer ,Dielectric ,Condensed Matter Physics ,Corrosion ,Metal ,Chemical engineering ,chemistry ,visual_art ,Halogen ,visual_art.visual_art_medium ,General Materials Science ,Isostructural ,Triboelectric effect - Abstract
The output performance of triboelectric nanogenerators (TENGs) basically depends on the inherent nature of friction electrode materials. In this work, a series of isostructural Cu(I)-CPs (coordination polymers) with different terminal coordination groups from the same main group, formulated as [Cu(μ3-H3ttc)X]n (1: X = Cl, 2: X = Br and 3: X = I; H3ttc = trithiocyanuric acid), were chosen as the friction electrode materials to fabricate TENGs (Cl-, Br- and I-TENG based on compounds 1–3, respectively) to clearly clarify the influence of chemical microcomposition on the output performance of TENGs. The results indicated that the polarity of the compounds determined from dielectric constants was greatly affected by the halogen elements, which plays a decisive role in the output performance of the TENGs. I-TENG based on the Cu(I)-CP containing the least electronegative halogen atoms but with the largest polarity had the largest output performance, followed by Br- and Cl-TENG. As a practical application, I-TENG was used as a power source to construct a self-powered anticorrosion system to protect metal materials from corrosion.
- Published
- 2022
37. Circ0008399 Interaction with WTAP Promotes Assembly and Activity of the m6A Methyltransferase Complex and Promotes Cisplatin Resistance in Bladder Cancer
- Author
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Guosong Jiang, Liang Wang, Wenjie Wei, Xiaoping Zhang, Hui Zhang, Xingyuan Xiao, Jiayin Sun, Chao Huang, Yangkai Jiang, and He Zhong
- Subjects
Cisplatin ,Cancer Research ,Messenger RNA ,Chemistry ,Methyltransferase complex ,Methylation ,TNFAIP3 ,Oncology ,Downregulation and upregulation ,Apoptosis ,Cancer research ,medicine ,Initiation factor ,medicine.drug - Abstract
Cisplatin (CDDP)-based chemotherapy is the first-line treatment for muscle-invasive and metastatic bladder cancer, yet most patients rapidly develop resistance. N6-methyladenosine (m6A) methylation is a pervasive RNA modification, and its specific role and potential mechanism in the regulation of CDDP chemosensitivity in bladder cancer remain unclear. Furthermore, studies have not yet fully elucidated whether circular RNA (circRNA) can directly regulate m6A modification of mRNA. Here we report upregulation of a novel circRNA, hsa_circ_0008399 (circ0008399), by eukaryotic translation initiation factor 4A3 (EIF4A3) in bladder cancer tissues and cell lines. Functionally, circ0008399 inhibited apoptosis of bladder cancer cells. Mechanistically, circ0008399 bound Wilms' tumor 1–associating protein (WTAP) to promote formation of the WTAP/METTL3/METTL14 m6A methyltransferase complex. Circ0008399 increased expression of TNF alpha-induced protein 3 (TNFAIP3) by increasing its mRNA stability in an m6A-dependent manner. In patients with bladder cancer, high expression of circ0008399 and WTAP was associated with poor outcomes. Importantly, activation of the circ0008399/WTAP/TNFAIP3 pathway decreased bladder cancer chemosensitivity to CDDP, and targeting the circ0008399/WTAP/TNFAIP3 axis enhanced the CDDP efficacy. Collectively, these findings give novel insights into circRNA-mediated regulation of m6A modifications and provide potential therapeutic targets for bladder cancer. Significance: A newly characterized circRNA circ0008399 binds WTAP to modulate expression of target RNA through m6A modification and reduce cisplatin sensitivity in bladder cancer, implicating the potential therapeutic value of targeting this axis.
- Published
- 2021
38. Diaminodiacid bridge improves enzymatic and in vivo inhibitory activity of peptide CPI-1 against botulinum toxin serotype A
- Author
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Dai Qiuyun, Yu Yunzhou, Shuo Yu, Xianghua Xiong, Jia Liu, Junjie Yue, Jintao Shen, and Chao Huang
- Subjects
chemistry.chemical_classification ,Peptide ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Trypsin ,Inhibitory postsynaptic potential ,Immunoglobulin light chain ,01 natural sciences ,Botulinum toxin ,0104 chemical sciences ,Amino acid ,Enzyme ,chemistry ,Biochemistry ,In vivo ,medicine ,0210 nano-technology ,medicine.drug - Abstract
The replacement of the disulfide bridge of CPI-1, a peptide inhibitor of light chain of Botulinum toxin serotype A, with the thioether-containing and biscarba-containing diaminodiacid bridge leads to a significant decrease in the degradation by trypsin and increase in the detoxification activity in vivo, the addition of hydrophobic or positive amino acid at C-terminus of modified peptides further improves the inhibitory activity.
- Published
- 2021
39. A Practical Synthesis of N ‐aryl/ N ‐alkyl 4‐Pyridones under Continuous Flow Technology
- Author
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Teng Liu, Yinjing Sun, Chao Huang, Qin Wu, Genrui Xu, and Weiqiang Li
- Subjects
chemistry.chemical_classification ,chemistry.chemical_compound ,chemistry ,Metal free ,Continuous flow ,Aryl ,Organic Chemistry ,Medicinal chemistry ,Alkyl - Published
- 2021
40. Double role of CoO Co4N hetero-nanocages as sulfur host for lithium-sulfur batteries
- Author
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Lü-Qiang Yu, Chao Huang, Jin-Lin Yang, Yi-Ming Lu, Shi-Xi Zhao, and Xiang-Tian Zeng
- Subjects
CoO-Co4N Hetero-nanocages ,Cathodes ,Materials science ,Nucleation ,chemistry.chemical_element ,02 engineering and technology ,Conductivity ,010402 general chemistry ,01 natural sciences ,Redox ,Energy storage ,Adsorption ,Nanocages ,Materials of engineering and construction. Mechanics of materials ,Sulfur host ,Li-S Batteries ,Metals and Alloys ,021001 nanoscience & nanotechnology ,Sulfur ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Redox kinetic ,chemistry ,Chemical engineering ,TA401-492 ,Lithium ,0210 nano-technology - Abstract
Lithium-sulfur batteries (LiSBs) are widely deemed as the most promising energy storage devices to substitute for traditional Li-ion batteries. However, its wide application is impeded by the soluble lithium polysulfides (LiPSs), which is called the shuttle effect, and the irregular distribution of final product Li2S. Herein, based on the interfaces engineering, CoO Co4N hetero-nanocages are used as the sulfur host for LiSBs. Taking advantage of the polarity of CoO with the conductivity of Co4N, CoO Co4N nanocages not only can provide large void space for sulfur volume fluctuation, but also can adsorb polysulfides and simultaneously regulate the nucleation of solid Li2S by the ‘trapping-diffusion-conversion’ mechanism, which significantly enhances the redox kinetic of LiSBs and the utilization of active materials. Eventually, LiSBs with CoO Co4N nanocages host exhibit higher rate capacity (737 mAh·g−1 at 2 C) and cycling stability (662 mAh·g−1 at 1 C after 350 cycles). Even when the areal sulfur loading is as high as 3.0 mg cm−2, a high capacity of 713 mAh·g−1 can still be achieved after 100 cycles at 0.2 C. This host with sufficient polar-conductive interfaces expands ‘trapping-diffusion-conversion’ concept for the design of fast kinetic and high performance LSBs.
- Published
- 2021
41. Retinal Astrocytes and Microglia Activation in Diabetic Retinopathy Rhesus Monkey Models
- Author
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Asad Jahangir, Chao Huang, Xiaoli Wei, Ting Pan, Jingfei Chen, Wentao Liu, Qihui Luo, Zhengli Chen, and Yu Xia
- Subjects
medicine.medical_specialty ,endocrine system diseases ,Calbindin ,Streptozocin ,Diabetes Mellitus, Experimental ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Internal medicine ,medicine ,Animals ,Retina ,Diabetic Retinopathy ,biology ,Glial fibrillary acidic protein ,business.industry ,nutritional and metabolic diseases ,Retinal ,Diabetic retinopathy ,Streptozotocin ,medicine.disease ,Macaca mulatta ,Sensory Systems ,Ophthalmology ,Diabetes Mellitus, Type 1 ,Endocrinology ,medicine.anatomical_structure ,Diabetes Mellitus, Type 2 ,chemistry ,Astrocytes ,biology.protein ,Microglia ,NeuN ,business ,Neuroglia ,Parvalbumin ,medicine.drug - Abstract
PURPOSE To assess the retinal neurodegeneration in type-1 diabetes mellitus (T1DM) and type-2 diabetes mellitus (T2DM) rhesus monkeys, and to investigate whether alterations of glial cells occur in the early stage of diabetic retinopathy (DR). MATERIAL AND METHODS T1DM rhesus monkeys were established by daily intravenous injections of streptozotocin (STZ, 25 mg/kg body weight) in citrate buffer (pH 4.5) for 5 days, while T2DM rhesus monkeys were induced by feeding with high-fat diet. The period of DR in rhesus monkeys was evaluated by fundoscopy and optical coherence tomography (OCT). Afterward, the morphological changes of inner neurons and glial cells in the retina were detected by immunofluorescence (IF). RESULTS When compared with the control groups, no difference was observed in both T1DM and T2DM by fundus photographs, while slight exudation and effusion in the blood vessels of retina of rhesus monkeys were found by OCT in DM rhesus monkeys. In addition, the expression of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adaptor molecule (Iba1) were significantly increased in both T1DM (P
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- 2021
42. Galectin-3 Derived from HucMSC Exosomes Promoted Myocardial Fibroblast-to-Myofibroblast Differentiation Associated with β-catenin Upregulation
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Hua Wang, Chao Huang, Jiejie Li, Xiangdong Zhao, Qinyu Guo, Yuanyuan Zhao, Wei Zhu, and Mei Wang
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medicine.diagnostic_test ,Chemistry ,Mesenchymal stem cell ,Cell Biology ,Cell biology ,medicine.anatomical_structure ,Downregulation and upregulation ,Western blot ,Galectin-3 ,In vivo ,Catenin ,medicine ,Fibroblast ,Myofibroblast ,Developmental Biology - Abstract
Background and objectives Galectin-3 promotes fibroblast-to-myofibroblast differentiation and facilitates injury repair. Previous studies have shown that exosomes derived from human umbilical cord mesenchymal stem cells (hucMSC-ex) promote the differentiation of myocardial fibroblasts into myofibroblasts under inflammatory environment. Whether hucMSC-ex derived Galectin-3 (hucMSC-ex-Galectin-3) plays an important role in fibroblast-to-myofibroblast differentiation is the focus of this study. Methods and results Galectin-3 was knocked-down by siRNA in hucMSCs, and then exosomes were extracted. Fibroblasts were treated with LPS, LPS+hucMSC-ex, LPS+negative control-siRNA-ex (NC-ex), or LPS+ Galectin-3-siRNA-ex (si-ex) in vitro. The coronary artery of the left anterior descending (LAD) branch was permanently ligated, followed by intramyocardial injection with phosphate buffered saline(PBS), hucMSC-ex, hucMSC-NC-ex, or hucMSC-si-ex in vivo. Western blot, RT-PCR, and immunohistochemistry were used to detect the expression of markers related to fibroblast-to-myofibroblast differentiation and inflammatory factors. Migration and contraction functions of fibroblasts were evaluated using Transwell migration and collagen contraction assays, respectively. β-catenin expression was detected by western blot and immunofluorescence. The results showed that hucMSC-ex increased the protein expression of myofibroblast markers, anti-inflammatory factors, and β-catenin. HucMSC-ex also reduced the migration and promoted the contractility of fibroblasts. However, hucMSC-si-ex did not show these activities. Conclusions HucMSC-ex-Galectin-3 promoted the differentiation of cardiac fibroblasts into myofibroblasts in an inflammatory environment, which was associated with increased β-catenin levels.
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- 2021
43. High Chemoselectivity in the Construction of Aryl Methyl Sulfones via an Unexpected C–S Bond Formation between Sulfonylhydrazides and Dimethyl Phosphite
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Xiang Shen, Yixian Li, Fei-Xiang Cheng, Chao Huang, Shi-Wen Yu, Teng Liu, and Jian-Jun Liu
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chemistry.chemical_classification ,chemistry.chemical_compound ,chemistry ,Aryl ,Organic Chemistry ,Methyl sulfones ,Dimethyl phosphite ,Chemoselectivity ,Bond formation ,Medicinal chemistry ,Catalysis ,Alkyl - Abstract
A highly chemoselective route to aryl methyl sulfones via an unexpected C–S bond formation between sulfonylhydrazides and dimethyl phosphite catalyzed by NaI under mild conditions has been established. This transformation provides an alternative and metal-free pathway to acquire various aryl methyl sulfones in good to excellent yields. Notably, dimethyl phosphite was employed as a stable and readily available alkyl source.
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- 2021
44. Distribution and Sources of Particulate Organic Matter in the Northern South China Sea: Implications of Human Activity
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Chunqing Chen, Chao Huang, Peiwang Bian, Shuwen Zhang, Fajin Chen, Qibin Lao, and Qingmei Zhu
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chemistry.chemical_classification ,Total organic carbon ,δ13C ,Stable isotope ratio ,Heterotroph ,Ocean Engineering ,δ15N ,Oceanography ,chemistry ,Environmental chemistry ,Environmental science ,Organic matter ,Eutrophication ,Isotope analysis - Abstract
In order to understand origin and fate of particulate organic matter, the isotopic composition (δ13C and δ15N), total organic carbon content, total nitrogen content, and C/N ratios were measured for suspended particulate organic matter (POM) collected from the northern South China Sea (NSCS) during summer. Our study revealed that δ13C generally decreased from land to sea, and elevated δ13C occurred at the nearshore stations, suggesting that POC was mainly contributed from the eutrophic level and microbial activity. Moreover, the distribution of δ15N values were complicated, and heterotrophic modification was responsible for higher δ15N in the nearshore stations. These distribution patterns of δ13C and δ15N in the nearshore stations may be associated with the intensification of human activity in the coast. Based on the Stable Isotope Analysis in R model, 65% of POM was contributed by marine organic matter in the NSCS, 20% by terrestrial inputs, and 15% by freshwater algae.
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- 2021
45. Accelerating C2+ alcohols synthesis from syngas by simultaneous optimizations of CO dissociation and chain growth over CuCo alloy catalyst
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Meiling Shui, Peiyu Ma, Bao Jun, Qun He, Wenjie Li, Chao Huang, Wenlong Wu, and Yisheng Tan
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Alcohol ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Combinatorial chemistry ,Dissociation (chemistry) ,0104 chemical sciences ,Catalysis ,chemistry.chemical_compound ,chemistry ,Alcohol synthesis ,0210 nano-technology ,Selectivity ,Co activation ,Alloy catalyst ,Syngas - Abstract
With regard to the reaction of higher alcohol synthesis (HAS), the optimizations of activity and selectivity towards C2+ alcohol are restricted by the improper equilibrium in two different CO activation pathways and chain growth capacity. Herein, we find that delibrately controlling the compositions of catalysts is an effective strategy to achieve the equilibrium of CO activation pathways and promote the chain growth. As a result, the optimized Cu0.25Co0.75 alloy catalyst can achieve a large proportion of higher alcohol in alcohol products (C2+OH/MeOH = 4.40), together with high CO conversion of 71.27% and space-time-yield of 147.65 g kg−1 h−1. The mechanistic studies suggest that the good performance of Cu0.25Co0.75 catalyst is attributed to the synergistic effect between alloyed Cu and Co.
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- 2021
46. Polysaccharide supramolecular hydrogel microparticles based on carboxymethyl β-cyclodextrin/chitosan complex and EDTA-chitosan for controlled release of protein drugs
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Chao Huang, Ying Wang, WeiCong Zhang, Juan Guo, and Yong Hu
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Polymers and Plastics ,Supramolecular chemistry ,02 engineering and technology ,010402 general chemistry ,Polysaccharide ,01 natural sciences ,Chitosan ,chemistry.chemical_compound ,Materials Chemistry ,medicine ,Bovine serum albumin ,chemistry.chemical_classification ,Aqueous solution ,Cyclodextrin ,biology ,General Chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Controlled release ,0104 chemical sciences ,chemistry ,biology.protein ,Swelling ,medicine.symptom ,0210 nano-technology ,Nuclear chemistry - Abstract
New pH-sensitive polysaccharide supramolecular hydrogel microparticles (HMPs) were constructed based on carboxymethyl β-cyclodextrin/chitosan inclusion complex (CM-β-CD/CS) and EDTA-chitosan (EDCS) in aqueous solution. The incorporation of components in HMPs was characterized by ultraviolet, fluorescence and FTIR spectroscopy. The morphology measurements of HMPs were investigated by particle size analyzer and fluorescence microscope. These results showed that CM-β-CD was integrated on the CS by electrostatic interactions, while the hydrogen bonding between CM-β-CD/CS complex and EDCS resulted in the formation of self-assembled HMPs. The swelling and shear rheological behaviors of the HMPs were also investigated, confirming the pH sensitive of the HMPs. In vitro drug release behaviors of the HMPs conducted in simulated gastric fluid (pH 1.2) and simulated intestinal fluid (pH 7.4) were studied by using bovine serum albumin (BSA) as model protein drug. Results indicated that the BSA-loaded HMPs exhibit highlighted pH-dependent release characteristics and the release of BSA from the HMPs could be described by the Ritger–Peppas model equation, suggesting that the HMPs might be formulated to be a promising candidate for the release of protein drugs.
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- 2021
47. Activated Nrf-2 Pathway by Vitamin E to Attenuate Testicular Injuries of Rats with Sub-chronic Cadmium Exposure
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Chao Huang, Zhuo Chen, Hongrui Guo, Fengyuan Wang, Zhengli Chen, Huidan Deng, Zhicai Zuo, Yi Geng, Kejie Chen, Wentao Liu, Ping Ouyang, Hengmin Cui, Yang Zhuangzhi, and Jing Fang
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Male ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Clinical Biochemistry ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,Antioxidants ,Rats, Sprague-Dawley ,Inorganic Chemistry ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,Testis ,medicine ,Animals ,Vitamin E ,0105 earth and related environmental sciences ,0303 health sciences ,business.industry ,GCLM ,030302 biochemistry & molecular biology ,Biochemistry (medical) ,General Medicine ,Glutathione ,Spermatozoa ,Sperm ,Rats ,Oxidative Stress ,GCLC ,chemistry ,Toxicity ,Reproductive toxicity ,business ,Oxidative stress ,Cadmium - Abstract
Cadmium (Cd), a heavy metal element, cumulates in the testis and can cause male reproductive toxicity. Although vitamin E (VE) as one of potential antioxidants protects the testis against toxicity of Cd, the underlying mechanism remained uncompleted clear. The aim of this study was to investigate whether the Nrf-2 pathway is involved with the protective effect of VE on testicular damages caused by sub-chronic Cd exposure. Thirty-two SD rats were divided into four groups and orally administrated with VE and/or Cd for 28 consecutive days: control group, VE group (100 mg VE/kg), Cd group (5 mg CdCl2/kg), and VE + Cd group (100 mg VE/kg + 5 mg CdCl2/kg). The results showed that 28-day exposure of Cd caused accumulation of Cd, histopathological lesions, and alternations of sperm parameters (elevated rate of abnormal sperm, decreased count of sperm, declined motility, and viability of sperm). Moreover, the rats exposed to Cd showed significant oxidative stress (increased contents of MDA and decreased levels or activities of T-AOC, GSH, CAT, SOD and GSH-Px) and inhibition of Nrf-2 signaling pathway (downregulation of Nrf-2, HO-1, NQO-1, GCLC, GCLM and GST) of the testes. In contrast, VE treatment significantly reduced the Cd accumulation, alleviated histopathological lesions and dysfunctions, activated Nrf-2 pathway, and attenuated the oxidative stress caused by Cd in the testes of rats. In conclusion, VE, through upregulating Nrf-2 pathway, could protect testis against oxidative damages induced by sub-chronic Cd exposure.
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- 2021
48. Coordination‐Induced N−H Bond Splitting of Ammonia and Primary Amine of Cu I −MOFs
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Chao Huang, Yan Qiao, Jie Wu, Hongwei Hou, Kuan Gao, and Shasha Wang
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Primary (chemistry) ,010405 organic chemistry ,Chemistry ,Hydrogen bond ,Organic Chemistry ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Catalysis ,Dissociation (chemistry) ,0104 chemical sciences ,Ammonia ,chemistry.chemical_compound ,X-ray photoelectron spectroscopy ,Polymer chemistry ,Amine gas treating ,Density functional theory ,Powder diffraction - Abstract
We report a porous three-dimensional anionic tetrazolium based CuI -MOF 1, which is capable of cleaving the N-H bond of ammonia and primary amine, as well as the O-H bond of H2 O along with spontaneous H2 evolution. In the gas-solid phase reaction of 1 with ammonia and water vapor, CuI -MOF 1 was gradually oxidized to NH2 -CuII -MOF and OH-CuII -MOF, through single-crystal-to-single-crystal (SCSC) structural transformations, which was confirmed by XPS, PXRD and X-ray single-crystal diffraction. Density functional theory (DFT) demonstrated that CuI -MOF could lower N-H bond dissociation free energy of ammonia through coordination-induced bond weakening and promote H2 evolution by the reduction potential of 1. To our knowledge, this is the first example of MOFs that activate ammonia and amine in gas-solid manner.
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- 2021
49. Dynamic formation and transcriptional regulation mediated by phytohormones during chalkiness formation in rice
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Yi Su, Ke Huang, Chao Huang, Langtao Xiao, Jianhua Tong, Wanhuang Lin, Manlin Wei, Qin Xie, Jinke Xu, and Zhigang Huang
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Sucrose ,Transcription, Genetic ,Plant Science ,Biology ,Quantitative trait locus ,Caryopsis ,Endosperm ,Transcriptome ,Transcriptional regulation ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Auxin ,Dynamic formation ,Transcription factor ,Plant Proteins ,chemistry.chemical_classification ,Oryza sativa ,Research ,Gene Expression Profiling ,Botany ,Reproducibility of Results ,food and beverages ,Oryza ,Starch ,Chalkiness ,Cell biology ,Phytohormones ,Gene Ontology ,Phenotype ,chemistry ,QK1-989 ,Rice ,Transcription Factors - Abstract
Background Rice (Oryza sativa L.) Chalkiness, the opaque part in the kernel endosperm formed by loosely piled starch and protein bodies. Chalkiness is a complex quantitative trait regulated by multiple genes and various environmental factors. Phytohormones play important roles in the regulation of chalkiness formation but the underlying molecular mechanism is still unclear at present. Results In this research, Xiangzaoxian24 (X24, pure line of indica rice with high-chalkiness) and its origin parents Xiangzaoxian11 (X11, female parent, pure line of indica rice with high-chalkiness) and Xiangzaoxian7 (X7, male parent, pure line of indica rice with low-chalkiness) were used as materials. The phenotype, physiological and biochemical traits combined with transcriptome analysis were conducted to illustrate the dynamic process and transcriptional regulation of rice chalkiness formation. Impressively, phytohormonal contents and multiple phytohormonal signals were significantly different in chalky caryopsis, suggesting the involvement of phytohormones, particularly ABA and auxin, in the regulation of rice chalkiness formation, through the interaction of multiple transcription factors and their downstream regulators. Conclusion These results indicated that chalkiness formation is a dynamic process associated with multiple genes, forming a complex regulatory network in which phytohormones play important roles. These results provided informative clues for illustrating the regulatory mechanisms of chalkiness formation in rice.
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- 2021
50. The sub-inhibitory concentration of cinnamaldehyde resists Aeromonas hydrophila pathogenicity via inhibition of W-pili production
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Ping Ouyang, Chao Huang, Jing Fang, Jiehao Chen, Kaiyu Wang, Lizi Yin, Xiaoli Huang, Hongrui Guo, Li Tang, Weiming Lai, Zhengli Chen, Yi Geng, and Defang Chen
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0106 biological sciences ,Hemagglutination ,biology ,medicine.drug_class ,Chemistry ,Cell adhesion molecule ,010604 marine biology & hydrobiology ,Antibiotics ,Biofilm ,04 agricultural and veterinary sciences ,Adhesion ,Aquatic Science ,biology.organism_classification ,01 natural sciences ,Pilus ,Cinnamaldehyde ,Microbiology ,Aeromonas hydrophila ,chemistry.chemical_compound ,040102 fisheries ,medicine ,0401 agriculture, forestry, and fisheries ,Agronomy and Crop Science - Abstract
Aeromonas hydrophila is one of the most important pathogens of zoonotic importance globally, causing various diseases in humans and animals. With the increase of drug resistance and the emergence of multidrug-resistant strains, therapeutic effects of antibiotics against A. hydrophila are limited. Hence, novel agents and strategies are urgently needed to overcome these difficulties. Wavy pili (W-pili), one of the major adhesion molecules, play a crucial role in adhesion and hemagglutination in the pathogenesis of A. hydrophila and regarded as a potential target for therapy. In this study, cinnamaldehyde, a natural compound isolated from cinnamon, could inhibit the production of W-pili and its biological function at the sub-inhibitory concentration (sub-MIC). To determine the active mechanism of cinnamaldehyde, transmission electron microscopy, adhesion inhibition, hemagglutination, electrophoresis, semi-quantitative RT-PCR, biofilm assay, and fish immersion challenge were performed. The results showed that cinnamaldehyde can reduce the production of W-pili and the formation of biofilm without affecting the growth of A. hydrophila. Besides, cinnamaldehyde interfered with the synthesis of W-pili by downregulating the expression of the genes related to pili protein, thereby reducing the adhesion and hemagglutination ability of A. hydrophila and its adhesive ability to the gills and intestines of channel catfish. Therefore, cinnamaldehyde could be employed as a promising candidate for preventing and controlling A. hydrophila infection in aquaculture, especially in antibiotic-resistant strains.
- Published
- 2021
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