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2 results on '"Authier, Laurent"'

1. Gold Nanoparticle-Based Quantitative Electrochemical Detection of Amplified Human Cytomegalovirus DNA Using Disposable Microband Electrodes

Author

Authier, Laurent, Grossiord, Celine, Brossier, Pierre, and Limoges, Benoit

Subjects
Chemistry, Analytic -- Research, Cytomegaloviruses -- Identification and classification, Electrochemistry -- Usage, Gold -- Usage, Nanotechnology -- Usage, Chemistry
Abstract

An electrochemical DNA detection method has been developed for the sensitive quantification of an amplified 406-base pair human cytomegalovirus DNA sequence (HCMV DNA). The assay relies on (i) the hybridization of the single-stranded target HCMV DNA with an oligonucleotide-modified Au nanoparticle probe, (ii) followed by the release of the gold metal atoms anchored on the hybrids by oxidative metal dissolution, and (iii) the indirect determination of the solubilized [Au.sup.III] ions by anodic stripping voltammetry at a sandwich-type screen-printed microband electrode (SPMBE). Due to the enhancement of the [Au.sup.III] mass transfer by nonlinear diffusion during the electrodeposition time, the SPMBE allows the sensitive determination of [Au.sup.III] in a small volume of quiescent solution. The combination of the sensitive [Au.sup.III] determination at a SPMBE with the large number of [Au.sup.III] released from each gold nanoparticle probe allows detection of as low as 5 pM amplified HCMV DNA fragment.

Published
2001

2. Detection of Cationic Phenolic Derivatives at a Surfactant-Doped Screen-Printed Electrode for the Sensitive Indirect Determination of Alkaline Phosphatase

Author

Bernd Schöllhorn, Benoît Limoges, Laurent Authier, and AUTHIER, Laurent

Subjects
Detection limit, chemistry.chemical_classification, Dodecylbenzene, Inorganic chemistry, Cationic polymerization, Substrate (chemistry), Sulfonic acid, Analytical Chemistry, Electrochemical gas sensor, chemistry.chemical_compound, chemistry, Pulmonary surfactant, [CHIM] Chemical Sciences, Electrochemistry, Phenol
Abstract

The enzyme-label alkaline phosphatase (AP) commonly used in immunoassay was recently detected at subfemtomolar concentration with a screen-printed electrode (SPE) coated with a Nafion film (O. Bagel et al., Anal. Chem., 1997, 69, 4688–4694) through the catalytic transformation of an anionic substrate (aromatic monoester phosphate) to a cationic electroactive product P+ (phenol substituted by a cationic function). In order to improve the technique, a new cation-exchanger-doped SPE was developed and its analytical performances were evaluated with several cationic phenolic products. The doped SPEs were prepared from a carbon ink loaded with the sodium salt of dodecylbenzene sulfonic acid. The anionic surfactant was strongly retained within the dry ink and it allowed the accumulation of nanomolar concentrations of cationic phenol derivatives to occur through an ion-exchange process at the electrode surface. The accumulated phenol compounds were detected by the voltammetric oxidation of their phenolic function. Preliminary results obtained with AP (detection limit of ca. 10−14 M) appear promising for enzyme immunoassays.

Published
1998

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