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Bu çalışmanın amacı, etlik piliç yemlerinemonosodyum glutamat (MSG) ilavesinin etlik piliçlerde et kalitesi üzerindekiolası etkilerinin 8 hafta boyunca değerlendirmektir. Toplamda 300 adet günlükkarışık cinsiyetteki Abor-acre civcivleri, rastgele olarak 6 muamele ve her muamelede10 civciv olacak şekilde 5 tekerrürlü olarak deneme dizaynı kurulmuştur. Hembaşlangıç hem de son dönem yemlerinde tat arttırıcı katkı maddesi olarak 6farklı miktarda monosodyum glutamat (0,0; 0,25; 0,50; 0,75; 1,00 ve 1,25g/kg)eklendi. Et kalitesi değerlendirmesi için deneme süresi sonunda her tekerrürdendört civciv (cinsiyeti bilinmeyen) kesildi. Sonuçlar etin yağ ve ham proteiniçeriği ile uyluk kasının lezzetinin MSG' den önemli ölçüde etkilendiğiniortaya çıkardı, ancak oksidatif stabilite, nem, çözülme ve pişirme kaybıyemlerdeki uygulamadan etkilenmedi. Özetle, etlik piliç yemlerine MSG’nin 0,75g/kg’a kadar eklenmesiyle piliç etlerinin lezzeti arttırılmış ve MSG içerendiyetle birlikte etin yağ içeriği azaltılmıştır., The objective of this study was to evaluate theeffect of monosodium glutamate (MSG) inclusion in broiler diet on meat qualityof broiler chickens for 8 weeks. A total of 300 day old mixed sexed Abor-acrechicks were randomly allocated to six treatments with five replicates per treatmentand ten birds per replicate in a completely randomized experimental design. Sixadministration levels of monosodium glutamate (0.00, 0.25, 0.50, 0.75, 1.00 and1.25 g MSG/kg diet) were added as taste enhancing additive in the diet at bothstarter and finisher phases. Four birds (unsexed) per replicate wereslaughtered at the end of the trial period for meat quality evaluation. Theresult revealed fat and crude protein contents of the meat and palatability ofthe thigh muscle were significantly influenced by MSG, but the oxidativestability, moisture, thawing and cooking loss were not significantly influencedby the dietary treatments. In summary, the palatability of chicken meat frombroilers fed diet with MSG inclusion up to 0.75 g MSG/kg diet was enhanced, andfat content was reduced with dietary inclusion of MSG.

Lipopolysaccharide (LPS) is isolated from the genital tract of animals suffering from uterine damage and ovarian dysfunction. This study provides direct molecular evidence about the mechanism through which endotoxins cause reproductive disorders. Granulosa cells and ovaries were collected from immature mice treated with eCG or with eCG and LPS injection intraperitoneally. Normal large antral follicles were observed in ovaries obtained from eCG and LPS coinjected mice, and the morphology of the ovaries was similar to that observed in the control group. These antral follicles were not deemed atretic because few TUNEL-positive cells were observed. However, the granulosa cells of large antral follicles did not acquire the ability to respond to hCG stimulation. The number of ovulated oocytes was significantly lower in LPS-injected mice after superovulation compared to mice that were not exposed to LPS. The low reactivity was caused by the limited expression of the Lhcgr gene, which encodes the LH receptor in granulosa cells as well as an LPS-induced increase in the level of Dnmt1 expression. The methylation rate of the Lhcgr promoter region was significantly higher in granulosa cells obtained from the LPS treatment group compared with the control group. Together, these findings demonstrated that the decrease in the expression of Lhcgr due to LPS was a result of the epigenetic regulatory action of LPS. Our studies suggest that ovarian follicular cysts that is characterized by bacterial infection in humans and animals, is closely connected to the level of methylation of the Lhcgr promoter region.