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Milk is a basic source of human diets, which contain essential materials such as carbohydrates, protein, lipids, minerals, vitamins and essential amino acids. In order to keep milk temporarily fresh, some unethical activities are usually adapted to prevent the financial losses due to the spoilage of milk during its transportation and sale. The aim of study is to identifying the adulterants presents in the samples and compare them with the standard products. The method used for the detection of neutralizers in milk is rosalic acid and forthe detection of formalin there was used Hehner’s Test in this study. The study had done on seven type of packed milk (MILK PACK NESTLE, MILK PACK PLUS, QUDRAT, TARANG, TAZA, EVERY DAY and KHATIZ). Finally the result showed, neutralizer which are using for the neutralizing of acidity in milk were negative in mentioned samples, but formalin which is used for the extending of milk shelf life was positive in MILK PACK NESTLE and TAZA PACKED MILK samples. From total 42 samples, we detected adulteration of formalin in 12 (28-30%) samples. We are advising to Society for the rejections of MILK PACK NESTLE and TAZA PACKED MILK as use, and related administrations’ must control the above packed milk imports.

Chlorpyrifos (ChF) is an organophosphate pesticide that is widely used in agricultural fields and indoor for controlling pests. Aquatic ecosystems are the recipients of various pesticide residues due to leaching spray drift and agricultural runoff and pose toxicity for aquatic organisms. Therefore, the current study was designed to investigate the oxidative stress enzymes and histological alterations in the vital organs of tilapia due to ChF exposure. LC50 (24 h) was calculated as 52.78 μg/l by exposing tilapia with different acute concentrations of ChF. For assessment of sub-lethal toxicity of ChF, the fish were divided into four groups (ChF1, ChF2, ChF3, and control group). ChF1 group was treated with 1/15th of LC50, whereas ChF2 and ChF3 groups were treated with 1/10th and 1/5th of LC50, respectively for 14 days. After that, ChF induced changes in oxidative stress enzymes and histological alterations were evaluated. It was found that the level of glutathione S-transferase (GST), catalase (CAT), and superoxide dismutase (SOD) increased significantly in the liver of ChF-treated tilapia. Histological study of liver tissues showed an increased number of Kupffer cells, hydropic degeneration, necrosis, and hemorrhage. In the spleen of treated fish, increased melanomacrophage centers, necrosis, and congestion were detected. Disorganized muscle fibers, cardiac muscle fiber degeneration, and coagulative necrosis were observed in the heart of ChF-treated fish. It is concluded that sub-lethal concentrations of ChF can induce oxidative stress and histological alterations in the tissues of tilapia.

Theoretical energy levels, wavelengths and transitions probabilities of six-times ionized cesium (Cs VII) are calculated using multi-configuration-Dirac-Hartree-Fock (MCDHF) method. The present calculations have been performed for the 5s\(^2\)5p, 5s\(^2\)6s, 5s\(^2\)5d, 5s5p\(^2\) configurations using the GRASP2018 package. The electron correlation effects, Breit interaction and quantum electro dynamic effects have been considered in the calculations.

Picrotoxinin (1), a stable natural neurotoxin containing a highly oxygenated polycyclic rigid compound, was first isolated from berries of Anamirta cocculus (L.) Wight & Arn. (formerly known as Menispermum cocculus). The present work was aimed to study the modification of the complex polycyclic structure of picrotoxinin (1) by using microbial transformation. Initially various fungi, such as Macrophomina phaseolina, Cunninghamella blakesleeana, Aspergillus flavus, Aspergillus niger, Cephalosporium aphidicola, and Fusarium lini were screened for their ability to modify the structure of 1. Only one fungus Macrophomina phaseolina was able to catalyze the structural modification of picrotoxinin (1). The subsequent whole cell catalysis produced an intramolecular trans-esterified product 2. The structure of the transformed product was deduced by spectroscopic, and single-crystal X-ray diffraction analyses. A comparative study on convulsant activity of picrotoxinin (1) and its metabolite 2 was conducted in mice. Interestingly, metabolite 2 showed a delayed convulsant activity, as compared to the compound 1.

Spring maize is highly sensitive to low temperatures during the early development of seedlings and to high temperatures during its reproductive stage. Different strategies are being used to minimize the adverse effects of temperature extremes. Therefore, a field experiment was conducted to enhance the performance of spring hybrid maize by seed priming (3% MLE) and transplanting 20 and 30-day-old seedlings. Seed priming with moringa leaf extract (MLE) significantly enhanced stand establishment in both direct sowing and in transplanting, as indicated by the higher emergence percentage, emergence index, and lower time taken to start of emergence and mean emergence time. Minimum days from sowing to tasseling and silking were found in MLE primed 20-day-old seedlings grown in a nursery. However, all the agronomic parameters increased considerably with MLE priming of 20-day-old seedlings. Thus, MLE priming reduced chilling damage by improving stand establishment, whereas transplanting 20-day-old seedlings further enhanced the agronomic traits, yield, and quality of maize. However, the perfor-mance of maize plants from 30-day-old transplanted seedling and direct sowing was substandard.

The regulatory T cells (Treg cells) expressing CD4 + CD25 + FOXP3 + markers are indispensable for the initiation of immune homeostasis and tolerance to self-antigens in both mice and humans. A decrease in regulatory T cells leads to various autoimmune pathologies. Herein, we report three low molecular weight, small organic molecules as a new series of Treg proliferators TRP-1-3. These small molecules were tested for their proliferative effect on regulatory T cells. It was found that TRP-1 (Oleracein E) strongly accelerates the Treg proliferation in vitro in a concentration-dependent manner. The effect was evident for all subsets of Treg cells tested, including naturally occurring, thymus-derived and peripherally-induced or adaptive Treg, indicating an effect independent of the maturation site. Importantly, increased Treg cells numbers by TRP-1 correlated with improved CD4 + CD25 + FOXP3 + expression in vitro, while propidium iodide-based staining showed low TRP-1-induced cytotoxicity. Molecular docking plus simulation studies of these TRP-1-3 with IL-2R, mTOR and TCR receptors suggest a TCR-based Treg cells activation mechanism. Because of its high Treg cells activities and low cellular cytotoxicity, TRP-1-3 may be useful in stimulating ex-vivo/in-vivo, Treg cell-specific responses for therapeutic applications.

Understanding the proteolytic processing of polyprotein mediated by NS2B-NS3 protease contributes to the exploration of the mechanisms underlying infection of Japanese encephalitis virus (JEV), a zoonotic flavivirus. In this study, eukaryotic and prokaryotic cell models were employed to identify the cleavage sites mediated by viral NS2B-NS3 protease in JEV polyprotein. Artificial green fluorescent protein (GFP) substrates that contained the predicted cleavage site sequences of JEV polyprotein were expressed in swine testicle (ST) cells in the presence and absence of JEV infection, or co-expressed in E. coli with the recombinant NS2B-NS3 protease that was generated by fusing the N-terminal protease domain of NS3 to the central hydrophilic domain of NS2B. The cleavage of GFP substrates was examined by western blot. Among twelve artificial GFP substrates containing the cleavage site sequences predictively processed by host cell and/or NS2B-NS3 proteases, all sites were found to be cleaved by host cell proteases with different efficiencies. The sites at internal C, NS2A/NS2B, NS2B/NS3 and NS3/NS4A junctions, but not the sites at internal NS3, internal NS4A and NS4B/NS5 junctions were identified to be cleaved by JEV NS2B-NS3 protease. These data provide insight into the proteolytic processing of polyprotein, which is useful for understanding JEV replication and pathogenesis.

The spectroscopic data of tungsten (W) is important for plasma science, as it is used as a diverter and in ITER. We have recorded the spectrum of tungsten on a 1.5-m Wadsworth spectrograph in the 2100--4900 A wavelength range. The ground configuration of the neutral tungsten is 5d\({}^{4}\)6s\({}^{2}\). First excited configurations in the odd parity system are 5d\({}^{3}\)6s\({}^{2 }\)(6p + 7p + 5f + 6f) + 5d\({}^{4}\)6s6p. The 5d\({}^{4}\)6s6p configuration has already been studied. We have identified hundreds of lines in this spectrum and confirmed fifty nine levels of the 5d\({}^{4}\)6s6p configuration. Relativistic Hartree-Fock (HFR) and least squares fitted (LSF) parametric calculations were carried out to interpret the observed spectrum. We have found good agreement with the previously published data.

The effect of minor amounts of impurities on the course of chemical reactions is often overlooked. Analyzing commercial 1-dodecene feeds, hydroperoxides were identified as critical impurities. The influence of varying hydroperoxide concentrations in olefin feeds was systematically investigated, experimentally studying rhodium-catalyzed hydroformylation using a diphosphite ligand. A significant loss of n-aldehyde selectivity and linear-to-branched ratio (l/b) was observed for increasing hydroperoxide concentrations. Feeding of an additional ligand and/or purification of 1-dodecene restored the catalyst performance.

Multiconfiguration Dirac-Hartree-Fock (MCDHF) calculations and subsequent Relativistic Configuration Interaction (RCI) calculations have been performed for the 5s\({}^{2}\)5p\({}^{4}\), 5s\({}^{2}\)5p\({}^{3}\)6s and 5s5p\({}^{5}\) configurations of six times ionized cerium using GRASP2018 package. The electron correlation effects, (BI) interaction and Quantum Electrodynamics (QED) effects have been considered in the calculation.\ Energy levels, oscillator strength and transition probabilities among the transitions of 5s\({}^{2}\)5p\({}^{4}\), 5s5p\({}^{5}\) and 5s\({}^{2}\)5p\({}^{3}\)6s have been calculated. The calculated energy levels are compared with experimental data available and show good agreement with it.

In this paper the ground and first excited configurations of Ce VIII have been studied, both experimentally and theoretically. The spectra of cerium have been photographed using a spark source on a normal incident spectrograph, equipped with a grating having the reciprocal dispersion of 1.38 A/mm in the first order. The ground configuration has been established experimentally for the first time. The GRASP2018 package has been used to calculate energy levels, wavelengths and transition rates for the 5s\(^2\)5p\(^3\) and 5s\(^5\)p\(^4\) configurations. The electron correlation effects, Breit interaction and quantum electrodynamics effects have been considered in the calculation. The experimentally established energy levels have been compared with their theoretical values.

Four-times ionized cerium (Ce V) is isoelectronic with neutral xenon, having 5s25p6 1S0, the only level in the ground configuration. Previously reported energy levels and line classifications of the 5s25p5 (4f+5d+6d+6s and 6p) configurations were evaluated empirically by means of extended calculations and Boltzmann plots. Here, we have added a new configuration 5s5p64f to the previously reported work. The spectrum was recorded on a 3 m normal incidence vacuum spectrograph in the 300–1240 A region at the Antigonish laboratory (Canada). Theoretical calculations were performed using the pseudo-relativistic Hartree-Fock (HFR) configuration interaction method. The transition probabilities were calculated for the above-mentioned configurations. We also calculated the lifetimes of 5s25p54f metastable states using the MCDHF method.

Fine structure energy levels, transition probabilities and oscillator strength for terms belonging to the ground configuration, \(\rm 4s^{2}4p^{2}\) and the first exited odd parity configurations \(\rm 4s4p^{3}\), \(\rm 4s^24p5s\) and \(\rm 4s^{2}4p4d\) of Ge-I like ions have been calculated using Hartree-Fock method with relativistic correction incorporating large number of interacting configurations \(\rm[4s^{2}4p(4f+5p+5g), 4s4p^{2}(4d+5s), 4p^{3}(4d+5s)]\). Experimentally reported levels have been compared with theoretical results establishing energy parameters in the first eleven members of the sequence (Ge I-Mo XI). The configuration \(\rm 4s^{2}4p4d\) in Rb VI, Sr VII, and \(\rm 4s4p^3\) except \(\rm 4s4p^{3}\) \(\rm^{5}S_2\) level in Ge-I, are not observed yet. A systematic study of Slater parameters in the isoelectronic sequence enabled us to make precise predictions of the missing energy levels belonging to these configurations.

Biotransformation of a synthetic progestonic hormone dydrogesterone (1), C21H28O2, with a plant pathogenic fungus Macrophomina phaseolina yielded two new 2 and 3, and a known 4 metabolites. These analogues were identified as, 3β,11α-dihydroxy-5β,9β,10α-pregna-7-ene-6,20-dione (2), 15β-hydroxy-9β,10α-pregna-4,6-diene-3,20-dione (3), and 8α-hydroxy-9β,10α-pregna-4,6-diene-3,20-dione (4). Major structural changes were observed in metabolite 2. New metabolite 3 showed anti-inflammatory potential, and was found to be the potent inhibitor of intracellular reactive oxygen species (ROS) from whole blood phagocytes (IC50 = 4.2 ± 0.3 μg/mL), as compared to standard drug Ibuprofen (IC50 = 11.2 ± 1.9 μg/mL). The metabolites 2, 3, and 4 were found to be non-toxic to NIH-3T3 (CRL-1658) normal cell line. This indicated anti-inflammatory potential of resulting metabolites.

Energy levels and emission line wavelengths of high-Z materials are useful for impurity diagnostics in the next generation fusion devices. For this here we have calculated E1, M2 transitions, oscillator strengths, and transition probabilities for transitions among the terms belonging to the 2p63s2, 2p63s3p, 2p63p2 and 2p63s3d for the Magnesium like cerium (Ce XLVII) using the GRASP2K package based on the fully relativistic multi-configuration Dirac–Fock method. The electron correlation effects, Breit interaction and quantum electrodynamics effects to the atomic state wave functions and the corresponding energies have been taken into account.

The ground configuration of neutral tungsten is 5d46s2. The first excited configurations in the odd parity system are 5d36s2 (6p+7p+5f+6f)+5d46s6p. Further excitation leads to large number of configurations. However the configuration 5d56p belonging to the odd parity was obtained by doubly exciting the 5d46s6p configuration. Relativistic Hartree–Fock (HFR) and least squares fitted (LSF) parametric calculations were carried out to interpret the observed spectrum recorded on a 1.5-m Wadsworth spectrograph at our laboratory in the wavelength region 2300A to 4500A. We confirmed the earlier reported ground configuration 5d46s2 and excited configuration 5d46s6p. Almost fifty new transitions establishing 22 energy levels belonging to the configuration 5d36s26p have been identified.

Boerhavia procumbens is a medicinal herb used in the treatment of various ailments including asthma, cough and jaundice. The concentration of calcium (280.7 ± 0.0 mg/100 g, 102.3 ± 0.0 mg/100 g), potassium (240.4 ± 0.0 mg/100 g, 92.1 ± 0.0 mg/100 g), aspartic acid (30.9 ± 0.0 g/100 g, 26.1 ± 0.0 g/100 g), glutamicacid (25.2 ± 0.0 g/100 g, 17.7 ± 0.0 g/100 g) and alanine were the abundant in the stem and root than leaf while carbohydrate (79.9 ± 0.0%) was found higher in the leaf of B. procumbens. The leaf and root extracts revealed the presence of alkaloids, flavonoids, tannins and phenol. Leaf extract was found to be more active against S. aureus (11.5 ± 0.3 mm), C. michiganensis (12.1 ± 0.2 mm) and F. oxysporum (23.6 ± 0.2 mm) than the root extract of B. procumbens. It is concluded that stem and root of B. procumbens contain essential nutrients and the antimicrobial study encourages the use of leaf extract for the treatment of most common microbial diseases.Video Clip of Methodology:4 min 54 sec Full Screen Alternate

Dyes are usually present in trace quantities in the treated effluents of many industries. The effectiveness of adsorption for dye removal from wastewaters has made it an ideal alternative to other expensive treatment methods. Biosorption of acid yellow (AY17), a monoazo acid dye, was investigated using Typha angustata L. as biosorbent in a batch system with respect to initial pH, temperature, initial dye concentration, biosorbent dosage, and contact time. The biomass exhibited the highest dye uptake capacity at 303K, initial pH value of 2, the initial dye concentration of 150mg/L, biosorbent dosage of 0.5g and contact time of 40min. The extent of dye removal increased with increase in time, biosorbent dosage and decreased with increase in temperature. The equilibrium sorption capacity of the biomass increased on increasing the initial dye concentration up to 150mg/L and then started decreasing in the studied concentration up to 600mg/L. The experimental results have shown that the acidic pH favors the biosorption. Langmuir and Freundlich adsorption model is used for the mathematical description of the biosorption equilibrium and isotherm constants are evaluated at different temperatures. Equilibrium data fitted very well to the Freundlich model in the studied concentration (25‐600mg/L) and temperature (303‐323K) ranges. The pseudo-first- and second-order kinetic models were also applied to the experimental data. The results indicated that the dye uptake process followed the pseudosecond-order rate expression and the adsorption rate constants increased with increasing concentration. The adsorption results in this study indicated that all the adsorbents were attractive candidates for removing acidic dyes from dye wastewater.

This re search work was carried out to quantify the total phenolics, flavonoids and carotenoides, vitamin C contents, reported as antioxidants, in the extracts of four locally available varieties of durian. Fruit was extracted using dichloromethane: pentane (1:1 v/v) and subjected to study the various quality parameters. The total phenolic contents from durian fruit extract, determined following the Folin-ciocalteu assay were found in the range of 690.62-998.29 mg/L, showing the significant inter-varietals variations. The total flavonoids and vitamin C contents from fruit extract were found in the range of 211.36-220.34 and 18.87-25.1 mg/L, respectively. Whereas, the total carotenoides contents of fruit extract were found in a low concentrations i.e. 0.05-0.08 mg/L. The statistical analysis showed significant (p 0.05) variation in total flavonoids contents among different varieties of durian. Caffeic acid and quercetin were the dominant antioxidant substances found in durian. In conclusion, the bioactivity of ripe durian was high and the total polyphenols were the main contributors to the overall antioxidant capacity.

A study was conducted in Jamber Khurd, a unioncouncil of Tehsil Pattoki, district Kasur in Punjab province,Pakistan, in order to determine the effects of using pollutedwater for irrigation which disturbed the quality of groundwater and then its ultimate effects on the environment andhealth of common man living in the area. Different waterquality parameters were studied in ground water samples thatincludes physico-chemical variables (pH, temperature,dissolved oxygen, conductivity, turbidity and total dissolvedsolids), anions (carbonates, bicarbonates and chlorides), cations(sodium, calcium and magnesium), biological variables liketotal coliform (faecal coliform and E- coli), heavy metals(manganese, nickel, chromium, lead, copper, cobalt, iron andzinc), sodium absorption ratio and residual sodium carbonate.The results were compared with National Environment QualityStandards. The results of the study show that, the use ofpolluted water increases the value of conductivity, totaldissolved solids, sodium absorption ratio and residual sodiumcarbonate in ground water and exceeds the acceptable limits ofNational Environment Quality Standards. The increase invalues was statistically significant. After detailed survey byconducting interview, it was concluded that the use of pollutedwater not only degraded the ground water quality but also havea severe health hazard on the residents of the area. Theconcentration of heavy metals was also found to be higher thanacceptable range. Possible recommendations were given, inorder to protect the area from pollution degradation.

Background: Chronic suppurative otitis media (CSOM) is an ever growing concern of preventable hearing loss and is a major health concern especially in tropical countries. Lack of cleanliness, overcrowding, poverty, malnutrition and medical ignorance are few of the contributing factors. The microbiological flora is constantly changing due to indiscriminate use of antibiotics with rapid change in the antibiotic sensitivity patterns. Hence, this study was undertaken to determine the epidemiological profile, bacteriological profile and the antibiotic sensitivity pattern which would help in establishing a protocol in management of CSOM patients in the hospital.Methods: A total of 100 patients of CSOM attending the ENT Outpatient Department of The Oxford medical College, Hospital and Research Centre were included in the study. The samples were immediately sent to the Microbiology laboratory for further processing according to standard procedure. Results: Out of 100 cases, 74 were active mucosal type and 26 were active squamous, with Pseudomonas species (33.6%) being the predominant organism. Staphylococcus aureus was the next common organism with MSSA (16.3%) and MRSA (12.5%), CONS were 6.7%. Among the 28 gram-negative bacilli, Klebsiella pneumoniae (7.7%), was followed by Escherichia coli (5.7%), and Proteus mirabilis (4.8%).Conclusions: Pseudomonas species and Staphylococcus aureus are the commonest organisms of CSOM. Ciprofloxacin can be used as a topical agent for treating gram-negative bacilli. Among systemic antibiotics- Linezolid, Amikacin and Imepenam remains the drug of choice.

The effect of different processing methods (soaking in water or solutions of sodium bicarbonate, citric acid, soaking plus cooking, and germination) on anti-nutritional factors (phytic acid, total polyphenols, tannins, and hydrocyanic acid) of red kidney bean was studied. The anti-nutritional factors were reduced significantly (P < 0.001) with processing techniques. Cyanide contents were most effectively (25%) reduced by cooking after soaking in sodium bicarbonate solution, followed by germination. The most drastic effect was noted on tannin contents. Cooking after soaking in either citric acid or sodium bicarbonate solutions almost eliminated it. However, simple soaking in water did not result in any reduction in tannin contents. Reduction in total polyphenols was 78.7% with cooking after soaking in sodium bicarbonate solution. Phytic acid contents were reduced only with germination treatment (42.6%), while the other treatments did not bring about any large reduction.

Effect of germination time on comparative sprout quality characteristics (proximate composition, ascorbic acid, phytic acid, invitro protein digestibility, protein solubility and sensory properties) was investigated. Sprouting significantly increased (P 5.0). Although, organoleptically the desi type chickpea sprouts were preferred over Kabuli type, the nutritional improvement due to sprouting was more pronounced in Kabuli chickpeas than their desi counterparts. Due to overall superior sprout quality, the kabuli type was more suitable for sprouting purposes.

Two fungal cultures Aspergillus niger and Cunninghamella blakesleeana were used for the biotransformation of methenolone enanthate (1). Biotransformation with A. niger led to the synthesis of three new (2-4), and three known (5-7) metabolites, while fermentation with C. blakesleeana yielded metabolite 6. Substrate 1 and the resulting metabolites were evaluated for their immunomodulatory activities. Substrate 1 was found to be inactive, while metabolites 2 and 3 showed a potent inhibition of ROS generation by whole blood (IC50=8.60 and 7.05μg/mL), as well as from isolated polymorphonuclear leukocytes (PMNs) (IC50=14.0 and 4.70μg/mL), respectively. Moreover, compound 3 (34.21%) moderately inhibited the production of TNF-α, whereas 2 (88.63%) showed a potent inhibition of TNF-α produced by the THP-1 cells. These activities indicated immunomodulatory potential of compounds 2 and 3. All products were found to be non-toxic to 3T3 mouse fibroblast cells.

We describe a continuous wave resonant acoustic sensor that has been optimized as a very sensitive in-line monitor for measuring the composition of precursor gases used in MOCVD processes. The precursor/carrier gas mixtures flow through a compact stainless steel acoustic chamber that is isolated from the acoustic transducers by a set of metallic diaphragms. The sensor has been successfully operated at supply line pressures from atmosphere down to 50 Torr with gas flow rates of up to 1600 sccm. The accuracy of the speed of sound measurement for hydrogen gas is better than 0.005%, even in a high noise and low pressure environment. Hydrogen, as well as nitrogen or argon carrier gases, are accommodated within the instrument's 1–5 kHz working frequency range. The instrument's sensitivity and stability are demonstrated with the laboratory data. Measurements of the dynamic response characteristics of the metalorganic bubbler lines at low pressure are also be presented. Application of the cell is general, encompassing any of the metalorganic and hydride materials typically used in MOCVD processes.

Background Aerva javanica (Burm. f.) Juss. ex Schult. (Amaranthacea) is traditionally used for the treatment of wound healings, cough, diarrhoea, ulcer and hyperglycaemia. The current study was aimed to fractionate and isolate bioactive compounds and ultimately to evaluate their anti-ulcereogenic potential. Results In order to achieve these aims, the fractionation, purifications and then biological potential determination of the isolated compounds was carried out. For purification purpose, initially extraction of the plant material was done with aqueous MeOH in the order of increasing polarity by using solvent-solvent extraction method. Phytochemical analysis revealed the presence of three compounds, 3-hydroxy-4 methoxybenzaldehyde (1), ursolic acid (2) and (E)-N-(4-hydroxy-3-methoxyphenethyl)-3-(4-hydroxy-3-ethoxyphenyl) acryl amide (3). Inhibition of urease activity of various fractions revealed that ethyl acetate fraction showed significant activity (P ) as compared to other fractions. (E)-N-(4-hydroxy-3-methoxyphenethyl)-3-(4-hydroxy-3-ethoxyphenyl) acryl amide (3) showed marked anti ulcer activity (P ). Conclusion These results suggested the mild potential of A. javanica against ulcer.

An important application of a quartz crystal microbalance is in the in-situ deposition monitoring of optical-dielectric materials. The useful life of a monitor crystal used in an optical deposition process is far shorter than experienced in most metal deposition processes. Furthermore, crystal failure occurs rather abruptly, preceded by a short period of increasing rate error. The surface morphology of the deposited film suggests that the stress at the quartz crystal-deposited film interface causes such a failure. An interfacial stress relief mechanism has been found to mitigate this problem. A new electrode structure has been devised that adds a compliant buffer layer of a suitable material, sandwiched between an adhesion layer and the electrode layer. Extensive laboratory tests indicate dramatic improvements in the monitor crystal's life, ranging from 250% with cryolite to over 600% with magnesium fluoride. In addition, better accuracy in the rate-thickness measurement is also achieved. Thus, overall process security is significantly enhanced.

In this paper, we present a numerical model based on the widely used finite element formulation to analyze in detail the effect of surface active agents on capillary–gravity wave parameters such as phase velocity and wave amplitude. Moreover, the effect of a physicochemical parameter, which is the ratio of surface concentration to surface tension is also considered. For a number of fluid samples covering a range of concentrations from 0 to 0.01 molar, the phase speed of waves propagating on the surface of the liquid is found to decrease monotonically as the concentration of the solution considered is increased up to a limit of 0.004 molar. This is attributed to the corresponding increase in capillary number. It is shown numerically that the Marangoni effects contribute to the interfacial dynamics for fluid with physicochemical parameter value greater than 0.5. Moreover, a grid refinement study shows accuracies and convergence orders of the numerical model.

Clean drinking water is the basic necessity for every human being, but about 1.1 billion people in the world lacked proper drinking water. There are many different types of water purification processes such as filtration, reverse osmosis, ultraviolet radiation, carbon absorption, but the most reliable processes are distillation and boiling. Water purification, such as distillation, is especially important in regions where water resources or tap water is not suitable for ingesting without boiling or chemical treatment. In design project It treats the water by combining different methods such as Filtration, Distillation and a technique called concentrated solar power (CSP). Distillation is literally the method seen in nature, whereby: the sun heats the water on the earth's surface, the water is turned into a vapor (evaporation) and rises, leaving contaminants behind, to form clouds. As the upper atmosphere drops in temperature the vapors cool and convert back to water to form water. In this project distillation is achieved by using a parabolic mirror which boils water at high temperature. Filtration is done by sand filter and carbon filter. First sand filter catches the sand particles and the carbon filter which has granules of active carbon is used to remove odor dissolved gases from water. This is the Pre-treatment of water. The filtered water is then collected in a water container at a focus of parabolic mirror where distillation process is done. Another important feature of designed project is the solar tracking of a parabolic mirror which increases the efficiency of a parabolic mirror [1],[2].

The quantitation of sialyl-Tn (STn) conjugated to keyhole limpet haemocyanin (KLH) can be determined by quantitating the amount of N-acetylneuraminic acid (NANA) released by acid or enzymatic digestion. An optimal 0.1 N H2SO4 acid hydrolysis at 80 degrees C results in quantitative release of NANA with minimal loss. A rapid isocratic method for the quantitation and separation of NANA is described using high-pH anion-exchange chromatography and pulsed amperometric detection (PAD). Multiple injection of NANA standard and/or samples containing protein led to a decrease in the PAD response which was corrected by addition of internal standard, alpha-2-keto-3-deoxyoctonate (KDO). The ratio of NANA/KDO peak area or peak height gives a linear response with increasing amount of NANA in the range 2.5-20 micro g/ml (r2 = 0.99). The limit of quantitation (LOQ) for NANA using this isocratic method is 1.9 micro g/ml (approximately 160 pmol/25 micro l injection). Based on the multiple determination the glycoconjugate, STn-KLH, showed a NANA content of 2.9% (w/w). Acid hydrolysis and the sialidase treatment of STn-KLH both yielded a similar NANA content. The carrier protein, KLH, showed the absence of NANA. The stability of glycoconjugate STn-KLH was monitored by a gradient method which separated possible degradation products STn-crotyl, NANA and GalNAc. Subjecting the glycoconjugate STn-KLH to various stress conditions of temperature, pH and oxidation does not result in any release of sialic acid, GalNac and STn-crotyl group.

The ImmunoDetection PG (protein G) cartridge which is based on perfusion chromatography has been evaluated for the quantitation of murine monoclonal antibody (IgG1) in biological fluids. The results indicate a low intra-day coefficient of variation (C.V.)or = 5% and a sample analysis time of 5.5 min. A standard curve generated using injection of known amounts of monomeric antibody against the eluted peak area shows linearity (r2 = 0.99) over the 0.1-100 micrograms range. The minimum detectable concentration (MDC) at 280 nm is about 2 micrograms/ml which can be decreased to 200 ng/ml if the antibody elution is monitored at 214 nm. Under these conditions the C.V. isor = 5%. Accuracy of the quantitation is independent of operator, solution composition, sample pH and the ionic strength, all of which are factors to consider in performing immunoassays. Antibody samples containing aggregated and fragmented Fc regions of the antibody will interfere with this method of quantitation. An optimized validated method for the quantitation of antibody is described. This mode of perfusion chromatography could be applied in the biopharmaceutical field due to its speed, efficiency and sensitivity.

Renilla Luciferase reporter gene (rLuc) GL4.82 and GL4.13 promoter are key player in transfection, but precise knowledge of its targets in colon cancer remains limited. The aim of this study was to characterize the best transfection technique to produce a stable transfected colon DLD1 (colorectal adenocarcinoma cell line), therefore imaging based approaches were employed. DLD1 cells were transfected with a Plasmid (SV40-RLuc) carrying Renilla luciferase under the control of the SV-40 promoter, by using two different transfection techniques. Cells expressing the required DNA were isolated after antibiotic (Puramycin) selection. Clones of DLD-1/SV40-RLuc were produced using two different techniques (96 well plates and Petri dish) and their florescence intensity was recorded using IVIS machine (Calliper Life Sciences, Hopkinton, USA). Both techniques were characterized with the help of serial dilution technique. Results from this study substantiated that electroporation is the best. As expected, clones varied in their specific luciferase activity along with the dilutions. With the increase in cell concentration increase in intensity of florescence was recorded. Based on the results we are confident that this transfected cell line DLD-1/SV40-RLuc (colorectal adenocarcinoma cell line) is the best for further Orthotopic Xenotransplantation Studies and in-vivo experiments as well. Investigation shows that DLD1/SV-rLuc cells have gained little bit resistance against both drugs therefore further study is suggested to know the reasons.

The efficacy of the banana peel ( Musa sapientum ) biomass was tested for the removal of lead, copper, zinc and nickel metal ions using batch experiments in single and binary metal solution under controlled experimental conditions. It is found that metal sorption increases when the equilibrium metal concentration rises. At highest experimental solution concentration used (150 mg/L), the removal of metal ions were 92.52% for lead, 79.55% for copper, 63.23% for zinc and 68.10% for nickel while at lowest experimental solution concentration (25 mg/L), the removal of metal ions were 94.80% for lead, 86.81% for copper, 84.63% for zinc and 82.36% for nickel. Biosorption equilibrium isotherms were plotted for metal uptake capacity (q) against residual metal concentrations (C f) in solution. The q versus Cf sorption isotherm relationship was mathematically expressed by Langmuir and Freundlich models. The values of separation factor were between zero and one indicating favourable sorption for four tested metals on the biosorbent. The surface coverage values were approaching unity with increasing solution concentration indicating effectiveness of biosorbent under investigation. The non-living biomass of M. sapientum present comparable biosorption capacity for lead, copper, zinc and nickel metal ions with other types of biosorbent materials found in literature and is effective to remove metal ions from single metal solutions as well as in the presence of other co-ions with the main metal of solution.