256 results on '"A. Barka"'
Search Results
2. Synthesis of Ba doped ZnO‐Al2O3 nanocomposite from layered double hydroxide structure and their photocatalytic activity for the degradation of caffeine
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Alaâeddine Elhalil, Mohamed Abdennouri, M’hamed Sadiq, Yassine Kadmi, Lidia Favier, and Noureddine Barka
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Ba/ZnO‐Al2O3 ,Mixed metal oxides photocatalyst ,Photocatalytic property ,Caffeine degradation ,UV irradiation ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
In this research, BaCO3/ZnO‐Al2O3 nanocomposite photocatalyst at different amount of Ba (1, 3 and 5 wt%) was prepared from Zn‐Al‐CO3 layered double hydroxides precursors using ceramic process. A range of physicochemical techniques including X‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) with energy dispersive X‐ray analysis (EDX), were employed to characterize the as‐prepared catalysts. The photocatalytic activity of the catalysts was evaluated for the photocatalytic degradation of caffeine as a model of pharmaceutical pollutant in aqueous solutions under UV irradiation. Detailed photocatalytic experiments based on the effects of dopant amount, irradiation time, catalyst dose, initial pH and reuse were performed and presented in this study. The enhancement of photocatalytic activity was strongly dependent on the Ba amount and adsorption process. The 1%Ba/ZnO‐Al2O3 sample with high adsorption capacity showed the highest photocatalytic activity with a degradation efficiency of 98.2% after 70 min of irradiation. It showed a significantly higher rate of degradation compared to undoped, pure ZnO and standard Degussa P‐25 titanium dioxide. The photocatalyst showed high stability after three regeneration cycles.
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- 2018
3. The Base-Editing Enzyme APOBEC3A Catalyzes Cytosine Deamination in RNA with Low Proficiency and High Selectivity
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Emily K Schutsky, Peter Bailer, Tong Wang, Aleksia Barka, Kiara N. Berríos, and Rahul M. Kohli
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Mutagenesis ,Deamination ,Proteins ,RNA ,Uracil ,DNA ,General Medicine ,Biochemistry ,Article ,Cytosine ,chemistry.chemical_compound ,chemistry ,Cytidine Deaminase ,Nucleic acid ,Humans ,Molecular Medicine ,APOBEC3A - Abstract
Human APOBEC3A (A3A) is a nucleic acid-modifying enzyme that belongs to the cytidine deaminase family. Canonically, A3A catalyzes the deamination of cytosine into uracil in single-stranded DNA, an activity that makes A3A both a critical antiviral defense factor and a useful tool for targeted genome editing. However, off-target mutagenesis by A3A has been readily detected in both cellular DNA and RNA, which has been shown to promote oncogenesis. Given the importance of substrate discrimination for the physiological, pathological, and biotechnological activities of A3A, here we explore the mechanistic basis for its preferential targeting of DNA over RNA. Using a chimeric substrate containing a target ribocytidine within an otherwise DNA backbone, we demonstrate that a single hydroxyl at the sugar of the target base acts as a major selectivity determinant for deamination. To assess the contribution of bases neighboring the target cytosine, we show that overall RNA deamination is greatly reduced relative to that of DNA, but can be observed when ideal features are present, such as preferred sequence context and secondary structure. A strong dependence on idealized substrate features can also be observed with a mutant of A3A (eA3A, N57G) which has been employed for genome editing due to altered selectivity for DNA over RNA. Altogether, our work reveals a relationship between the overall decreased reactivity of A3A and increased substrate selectivity, and our results hold implications both for characterizing off-target mutagenesis and for engineering optimized DNA deaminases for base-editing technologies.
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- 2022
4. Natural Phosphates Characterization and Evaluation of their Removal Efficiency of Methylene Blue and Methyl Orange from Aqueous Media
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R. Elmoubarki, Meryem Assimeddine, M. Sadiq, Noureddine Barka, and Mohamed Abdennouri
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Aqueous medium ,Chemistry ,Environmental technology. Sanitary engineering ,Environmental sciences ,chemistry.chemical_compound ,adsorption ,methyl orange ,Methyl orange ,methylene blue ,GE1-350 ,natural phosphate ,Methylene blue ,TD1-1066 ,General Environmental Science ,Nuclear chemistry - Abstract
This study evaluated the capacity of a rock phosphate for the adsorption of organic dyes methylene blue MB and methyl orange MO in aqueous solution, in order to minimize the impact of these dyes on the environment. The physicochemical characterization of natural phosphates (NP) shows that its mineralogy is carbonate-fluorapatite, calcite and quartz, as demonstrated by X-ray diffraction. An infrared (IR) analysis completed the structural study by confirming the characteristic bands of a carbonated fluorapatite type B. The influence of adsorbent dose, pH, initial concentration and temperature of the dye solution on adsorption onto NP was studied. The experimental results show that MB is adsorbed almost entirely at an adsorbent dose of 1 g/L and at a more basic pH and that the Langmuir model describes its isotherm well. For MO, adsorption is performed at acidic pH, such that discoloration reaches 60% at pH 4 and NP adsorbent dose of 10 g/L. The maximum adsorbed amounts of MB (pH=9) and MO (pH=4) were found to be 9.54 and 1.09 mg/g, respectively. The kinetic data were analyzed to show that the pseudo-second-order model seems to be the most appropriate to describe the adsorption dynamics of both dyes on the naturel phosphate. The thermodynamic results show that the adsorption is endothermic for MB and exothermic for MO. So, rock phosphate shows a good potential as a sorbent for cationic dyes removal from wastewater.
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- 2021
5. Impact of Paraburkholderia phytofirmans PsJN on Grapevine Phenolic Metabolism
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Lidiane Miotto-Vilanova, Barbara Courteaux, Rosa Padilla, Fanja Rabenoelina, Cédric Jacquard, Christophe Clément, Gilles Comte, Céline Lavire, Essaïd Ait Barka, Isabelle Kerzaon, and Lisa Sanchez
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vitis vinifera ,beneficial bacterium ,phenolic compounds ,qrt-pcr ,uhplc-uv/dad-ms qtof ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Phenolic compounds are implied in plant-microorganisms interaction and may be induced in response to plant growth-promoting rhizobacteria (PGPRs). Among PGPR, the beneficial bacterium Paraburkholderia phytofirmans PsJN was previously described to stimulate the growth of plants and to induce a better adaptation to both abiotic and biotic stresses. This study aimed to investigate the impact of PsJN on grapevine secondary metabolism. For this purpose, gene expression (qRT-PCR) and profiling of plant secondary metabolites (UHPLC-UV/DAD-MS QTOF) from both grapevine root and leaves were compared between non-bacterized and PsJN-bacterized grapevine plantlets. Our results showed that PsJN induced locally (roots) and systemically (leaves) an overexpression of PAL and STS and specifically in leaves the overexpression of all the genes implied in phenylpropanoid and flavonoid pathways. Moreover, the metabolomic approach revealed that relative amounts of 32 and 17 compounds in roots and leaves, respectively, were significantly modified by PsJN. Once identified to be accumulated in response to PsJN by the metabolomic approach, antifungal properties of purified molecules were validated in vitro for their antifungal effect on Botrytis cinerea spore germination. Taking together, our findings on the impact of PsJN on phenolic metabolism allowed us to identify a supplementary biocontrol mechanism developed by this PGPR to induce plant resistance against pathogens.
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- 2019
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6. Controllable genome editing with split-engineered base editors
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Meiqi Luo, Caroline Bartman, Rahul M. Kohli, Niklaus H. Evitt, Junwei Shi, Rachel A. DeWeerd, Yemin Lan, Kiara N. Berríos, Aleksia Barka, Tong Wang, Diqiu Ren, and Abby M. Green
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Gene Editing ,Sirolimus ,Constitutively active ,DNA ,Nucleoside Deaminases ,Cell Biology ,Computational biology ,Biology ,Article ,Cytosine ,chemistry.chemical_compound ,Regulatory control ,Genome editing ,chemistry ,Deaminase activity ,Escherichia coli ,Nucleic Acid Conformation ,DNA Breaks, Double-Stranded ,CRISPR-Cas Systems ,Molecular Biology ,Biotechnology - Abstract
DNA deaminase enzymes play key roles in immunity and have recently been harnessed for their biotechnological applications. In base editors (BEs), the combination of DNA deaminase mutator activity with CRISPR-Cas localization confers the powerful ability to directly convert one target DNA base into another. While efforts have been made to improve targeting efficiency and precision, all BEs to date utilize a constitutively active DNA deaminase. The absence of regulatory control over promiscuous deaminase activity remains a major limitation to accessing the widespread potential of BEs. Here, we reveal sites that permit splitting of DNA cytosine deaminases into two inactive fragments, whose reapproximation reconstitutes activity. These findings allow for the development of split-engineered base editors (seBEs), which newly enable small-molecule control over targeted mutator activity. We show that the seBE strategy facilitates robust regulated editing with BE scaffolds containing diverse deaminases, offering a generalizable solution for temporally controlling precision genome editing.
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- 2021
7. Activités antioxydante et insecticide d’huiles essentielles de Mentha arvensis L. du Sénégal
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Cheikhna Hamala Sanghare, El Hadji Barka Ndiaye, Momar Talla Gueye, Papa Seyni Cissokho, Serigne Mbacké Diop, Abdoulaye Thiam, and Marie-Laure Fauconnier
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biology ,DPPH ,Mentha arvensis ,Context (language use) ,biology.organism_classification ,Menthone ,law.invention ,Steam distillation ,chemistry.chemical_compound ,Horticulture ,chemistry ,law ,Gas chromatography ,Menthyl acetate ,Essential oil - Abstract
Ce travail s'inscrit dans le contexte global de valorisation de la biodiversité des plantes aromatiques du Sénégal. L’objectif de l’étude était de déterminer la composition chimique d’huiles essentielles (HE) de Mentha arvensis L. et d’évaluer leurs activités antioxydante et insecticide. A cet effet, des feuilles de M. arvensis ont été récoltées dans la région de Dakar (Sénégal) et séchées à l'ombre puis leurs HE extraites par entrainement à la vapeur d'eau. Les HE obtenues ont été analysées par chromatographie en phase gazeuse (CPG) couplée à un détecteur à ionisation de flamme (FID) et un spectromètre de masse (MS). L’activité antioxydante des HE a été évaluée par la méthode d’inhibition du radical 2,2-diphényl-1-picrylhydrazyl (DPPH) tandis que l’activité insecticide a été testée par fumigation contre Sitophilus zeamais (Motsch.). Les résultats ont montré que les HE contiennent principalement du menthol (68,4%), de l’acétate de menthyle (16,9%) et de la menthone (5,0%). Leur activité antioxydante augmente avec la concentration, soient 38%, 57% et 73% d’inhibition respectivement pour 10, 25 et 50 mg/ml ; la CI50 est de 19,5 mg/ml. Les huiles présentent aussi une activité insecticide. Cette dernière bien qu’étant faible après 24 heures, augmente fortement au bout de 48 heures avec une mortalité maximale de 92%. Cette mortalité atteint des maximums de 98% pour 5 et 25 μl et 100% pour 50 et 75 μl après 72 heures. English title: Antioxidant and insecticidal activities of essential oils of Mentha arvensis L. from Senegal This work is part of the global context of valorization of the biodiversity of aromatic plants from Senegal. The aim of the study is to determine the chemical composition of essential oils (EOs) of Mentha arvensis L. and to evaluate their antioxidant and insecticidal activities. For this purpose, leaves of M. arvensis were harvested in Dakar region, Senegal and dried in the shade. EOs were extracted by steam distillation from dried leaves and analyzed by gas chromatography (GC) coupled to a flame ionization detector (FID) and a mass spectrometer (MS). The antioxidant activity of EOs was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method while the insecticidal activity was tested by fumigation against Sitophilus zeamais (Motsch.). The results revealed that EOs were dominated by menthol (68.4%), menthyl acetate (16.9%) and menthone (5.0%). The antioxidant activity assay showed that the inhibition increased with the concentration: 38%, 57% and 73% for 10, 25 and 50 mg/ml, respectively; the IC50 was of 19.5 mg/ml. The oils were also effective as insecticide. Mortalities were low after 24 hours and increased strongly after 48 hours with a maximum of 92%. They reached a maximum of 98% for 5 and 25 μl and 100% for 50 and 75 μl after 72 hours.
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- 2021
8. Aqueous fruit pulp extract of Adansonia digitata (L) protects against lead-acetate-induced hepato-renal damage in rat model
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Wusa Makena, Barka Ishaku, Sebastine Anthony Bazabang, Eduitem S. Otong, and Nathan Isaac Dibal
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Medicine (General) ,Science ,Pharmaceutical Science ,Medicine (miscellaneous) ,Renal function ,Adansonia ,Pharmacology ,Kidney ,Blood serum ,R5-920 ,medicine ,biology ,Chemistry ,biology.organism_classification ,Agricultural and Biological Sciences (miscellaneous) ,medicine.anatomical_structure ,Liver ,Lead acetate ,Creatinine ,Toxicity ,Adansonia digitata ,Liver function ,Succimer - Abstract
Background Adansonia digitata (L) fruit has a multi-purpose function one among many, is the antioxidant activities of the fruit by preventing oxidative stress. The effect of Adansonia digitata (L) fruit on lead-induced liver and kidney damage is not clear. Hence, the study was aimed to assessed the protective role of Adansonia digitata (L) fruits against lead acetate induced changes in the liver and kidney function test parameters and the histology of both organ in experimental rats. The rats were divided into five groups with five rats each. All the rats were administered with respective assigned treatment once daily for 6 weeks. Rats in groups I were administered with just distil water (2 ml/kg). Rats in groups II were administered with lead acetate (30 mg/kg) while rats in groups III–V were administered Adansonia digitatata (L) fruit extract (250 mg/kg and 500 mg/kg) and Succimer (5 mg/kg) respectively, then additionally challenged with lead acetate (30 mg/kg) immediately after. At the end of the administration, the blood serum from the experimental rats were used for biochemical analysis. Then, the the organs such as the liver and kidney collected for histological study. Results Rats administered with Lead acetate showed an increase in AST, ALP and ALT as well as increase in urea and creatinine level (p Adansonia digitatata (L) fruit prevented the effect (upsurge of serum, Urea, Creatinine, AST, ALP and ALT) of lead acetate. Rats administer with only Lead acetate revealed marked liver steatosis and the degeneration of the kidney glomerulus. The Adansonia digitatata (L) fruit extract and Succimer prevented the histological liver steatosis, as well as the degeneration of the glomerulus of the kidney cytoarchitecture. Conclusion The findings in this study suggest that Adansonia digitata fruits extract has a protective potentials against lead acetate induced liver and kidney toxicity by preventing the upsurge of liver function enzymes and kidney function parameters. Hence, Adansonia digitata fruits can serve as a natural plant agent that can prevent hepato-renal toxicity. Therefore, Adansonia digitata holds future prospects in preclinical framework to ameliorate organs toxicity for oral therapeutic applications.
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- 2021
9. Comparative adsorption of methyl orange on SO42- and SDS intercalated Mg-Fe layered double hydroxides
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A. Oussama, Fouzia Kzaiber, A. Elhalil, M. Abdennouri, F.Z. Mahjoubi, S. Charafi, Noureddine Barka, and Rachid Elmoubarki
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Chemistry ,Health, Toxicology and Mutagenesis ,Intercalation (chemistry) ,Public Health, Environmental and Occupational Health ,Layered double hydroxides ,Soil Science ,engineering.material ,Pollution ,Analytical Chemistry ,chemistry.chemical_compound ,Adsorption ,engineering ,Methyl orange ,Environmental Chemistry ,Waste Management and Disposal ,Water Science and Technology ,Nuclear chemistry - Abstract
This work investigates the synthesis of Mg-Fe layered double hydroxides intercalated by sulphate (Mg-Fe-SO4) or dodecylsulfate (Mg-Fe-SDS) and their capacity to adsorb methyl orange (MO), as a mode...
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- 2021
10. Antibody Epitope and Affinity Determination of the Myocardial Infarction Marker Myoglobin by SPR-Biosensor Mass Spectrometry
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Michael Przybylski, Pascal Wiegand, Michael O. Glocker, Loredana-Mirela Lupu, Günes Barka, Wolfgang Kleinekofort, Delia Mihoc, Frederik Barka, Friedemann Völklein, and Oliver Müller
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Spectrometry, Mass, Electrospray Ionization ,Hemeprotein ,medicine.drug_class ,Myocardial Infarction ,Peptide ,Monoclonal antibody ,Peptide Mapping ,Epitope ,Epitopes ,chemistry.chemical_compound ,Structural Biology ,medicine ,Animals ,Heme ,Spectroscopy ,chemistry.chemical_classification ,biology ,Myoglobin ,Chemistry ,Antibodies, Monoclonal ,Surface Plasmon Resonance ,Biochemistry ,biology.protein ,Target protein ,Antibody ,Antibodies, Immobilized ,Biomarkers - Abstract
Myoglobin (MG) is a biomarker for heart muscle injury, making it a potential target protein for early detection of myocardial infarction. Elevated myoglobin levels alone have low specificity for acute myocardial infarction (AMI) but in combination with cardiac troponin T have been considered highly efficient diagnostic biomarkers. Myoglobin is a monomeric heme protein with a molecular weight of 17 kDa that is found in skeletal and cardiac tissue as an intracellular storage unit of oxygen. MG consists of eight α-helices connected by loops and a heme group responsible for oxygen-binding. Monoclonal antibodies are widely used analytical tools in biomedical research and have been employed for immunoanalytical detection of MG. However, the epitope(s) recognized by MG antibodies have been hitherto unknown. Precise molecular identification of the epitope(s) recognized by antibodies is of key importance for the development of MG as a diagnostic biomarker. The epitope of a monoclonal MG antibody was identified by proteolytic epitope extraction mass spectrometry in combination with surface plasmon resonance (SPR) biosensor analysis. The MG antibody was immobilized both on an affinity microcolumn and a gold SPR chip. The SPR kinetic analysis provided an affinity-binding constant KD of 270 nM for MG. Binding of a tryptic peptide mixture followed by elution of the epitope from the SPR-MS affinity interface by mild acidification provided a single-epitope peptide located at the C-terminus [146-153] [YKELGFQG] of MG. The specificity and affinity of the epitope were ascertained by synthesis and affinity-mass spectrometric characterization of the epitope peptide.
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- 2020
11. Identification and Affinity Determination of Protein-Antibody and Protein-Aptamer Epitopes by Biosensor-Mass Spectrometry Combination
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Sascha Knauer, Wolfgang Kleinekofort, Loredana-Mirela Lupu, Michael Przybylski, Frederik Barka, Pascal Wiegand, Günes Barka, Julia B. Hennermann, Daria Holdschick, Stephan Rawer, Ebrahim Malek, Stefan Maeser, Andreas Hahn, Delia Mihoc, Friedemann Völklein, and Christina Uth
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QH301-705.5 ,Aptamer ,proteolytic epitope extraction ,Antibody Affinity ,SPR ,monoclonal ,Peptide ,Biosensing Techniques ,Review ,Antibodies ,Catalysis ,Epitope ,Protein–protein interaction ,Inorganic Chemistry ,Epitopes ,cross-immunoreactivity ,Animals ,Humans ,Physical and Theoretical Chemistry ,Surface plasmon resonance ,Biology (General) ,Molecular Biology ,QD1-999 ,Spectroscopy ,Antiserum ,chemistry.chemical_classification ,biology ,Chemistry ,Organic Chemistry ,interleukin-8 ,affinity determination ,General Medicine ,Aptamers, Nucleotide ,Surface Plasmon Resonance ,DNA aptamers ,chip-MALDI-mass spectrometry ,Computer Science Applications ,Biochemistry ,epitope structure determination ,Polyclonal antibodies ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,myoglobin ,biology.protein ,polyclonal protein antibodies ,Protein G - Abstract
Analytical methods for molecular characterization of diagnostic or therapeutic targets have recently gained high interest. This review summarizes the combination of mass spectrometry and surface plasmon resonance (SPR) biosensor analysis for identification and affinity determination of protein interactions with antibodies and DNA-aptamers. The binding constant (KD) of a protein–antibody complex is first determined by immobilizing an antibody or DNA-aptamer on an SPR chip. A proteolytic peptide mixture is then applied to the chip, and following removal of unbound material by washing, the epitope(s) peptide(s) are eluted and identified by MALDI-MS. The SPR-MS combination was applied to a wide range of affinity pairs. Distinct epitope peptides were identified for the cardiac biomarker myoglobin (MG) both from monoclonal and polyclonal antibodies, and binding constants determined for equine and human MG provided molecular assessment of cross immunoreactivities. Mass spectrometric epitope identifications were obtained for linear, as well as for assembled (“conformational”) antibody epitopes, e.g., for the polypeptide chemokine Interleukin-8. Immobilization using protein G substantially improved surface fixation and antibody stabilities for epitope identification and affinity determination. Moreover, epitopes were successfully determined for polyclonal antibodies from biological material, such as from patient antisera upon enzyme replacement therapy of lysosomal diseases. The SPR-MS combination was also successfully applied to identify linear and assembled epitopes for DNA–aptamer interaction complexes of the tumor diagnostic protein C-Met. In summary, the SPR-MS combination has been established as a powerful molecular tool for identification of protein interaction epitopes.
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- 2021
12. Chemically modified carbon-based electrodes for the determination of paracetamol in drugs and biological samples
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Noureddine Barka, Wafaa Boumya, Nawal Taoufik, and M. Achak
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Review Paper ,Chemistry ,Pharmaceutical samples ,digestive, oral, and skin physiology ,010401 analytical chemistry ,Pharmaceutical Science ,Nanotechnology ,RM1-950 ,02 engineering and technology ,Pharmacy ,Hepatic necrosis ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Modified electrodes ,Paracetamol ,Modified carbon ,Drug Discovery ,Electrode ,Electrochemistry ,Electrochemical sensors ,Therapeutics. Pharmacology ,0210 nano-technology ,Spectroscopy - Abstract
Paracetamol is a non-steroidal, anti-inflammatory drug widely used in pharmaceutical applications for its sturdy, antipyretic and analgesic action. However, an overdose of paracetamol can cause fulminant hepatic necrosis and other toxic effects. Thus, the development of advantageous analytical tools to detect and determine paracetamol is required. Due to simplicity, higher sensitivity and selectivity as well as costefficiency, electrochemical sensors were fully investigated in last decades. This review describes the advancements made in the development of electrochemical sensors for the paracetamol detection and quantification in pharmaceutical and biological samples. The progress made in electrochemical sensors for the selective detection of paracetamol in the last 10 years was examined, with a special focus on highly innovative features introduced by nanotechnology. As the literature is rather extensive, we tried to simplify this work by summarizing and grouping electrochemical sensors according to the by which manner their substrates were chemically modified and the analytical performances obtained., Graphical abstract Image 1, Highlights • An overview of the electrochemical sensors developed for the sensing of paracetamol in the last 10 years. • Carbon paste, glassy carbon and screen printed are the most investigated carbon-based electrodes. • Multiwalled carbon nanotubes-based composites are the most efficient modifiers for paracetamol detection. • Metal organic frameworks allowed the detection of paracetamol at picomolar levels. • Differential pulse voltammetry was the most useful to discriminate multiple compounds and improve sensitivity.
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- 2021
13. New Evidence of the Enhanced Elimination of a Persistent Drug Used as a Lipid Absorption Inhibitor by Advanced Oxidation with UV-A and Nanosized Catalysts
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Narcisa Vrinceanu, Raluca Maria Hlihor, Andrei Ionut Simion, Lacramioara Rusu, Ildikó Fekete-Kertész, Noureddine Barka, and Lidia Favier
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emerging water pollutants ,pharmaceutic compounds ,titania catalysts ,photocatalysis ,kinetics ,degradation ,mineralization ,toxicity ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
This work demonstrates new evidence of the efficient destruction and mineralization of an emergent organic pollutant using UV-A and titanium nanosized catalysts. The target compound considered in this work is the primary metabolite of a lipid regulator drug, clofibrate, identified in many studies as refractory during conventional wastewater treatment. The photocatalytic performance study was carried out in batch mode at laboratory scale, in aqueous suspension. Kinetic data showed that titanium dioxide P25 Aeroxide® exhibits the highest photocatalytic efficiency compared to the other investigated catalysts. Pollutant degradation and mineralization efficiencies strongly increased when decreasing the initial substrate concentration. Target molecules oxidized faster when the catalyst load increased, and the mineralization was enhanced under acidic conditions: 92% of mineralization was achieved at pH 4 after 190 min of reaction. Radical quenching assays confirmed that HO• and ( h vb + ) were the reactive oxygen species involved in the photocatalytic oxidation of the considered pollutant. In addition, further results revealed that the removal efficiency decreased in real water matrices. Finally, data collected through a series of phytotoxicity tests demonstrated that the photocatalytic process considerably reduces the toxicity of the treated solutions, confirming the process’s effectiveness in the removal of persistent and biorefractory emergent organic water pollutants.
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- 2019
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14. Klebsiella pneumoniae producing extended spectrum β-lactamase in Regional Military University Hospital of Oran, Algeria: antibiotic resistance, biofilm formation, and detection of blaCTX-M and blaTEM genes
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C. Benbrahim, M.S. Barka, A. Khadir, L. Benmahdi, and A. Zatout
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0301 basic medicine ,Imipenem ,biology ,Klebsiella pneumoniae ,General Chemical Engineering ,030106 microbiology ,Surgical wound ,biology.organism_classification ,Molecular biology ,03 medical and health sciences ,chemistry.chemical_compound ,Minimum inhibitory concentration ,030104 developmental biology ,chemistry ,Ampicillin ,Ticarcillin ,medicine ,Cefoxitin ,Ertapenem ,medicine.drug - Abstract
Background: Klebsiella pneumoniae is a bacterial pathogen commonly associated with severe nosocomial and community acquired infections especially through the acquisition of extended spectrum β-lactamases (ESβL) and biofilm formation capacity. The objectives of this study are to determine the prevalence of K. pneumoniae ESβL (KP-ESβL)-producing isolates in the Regional Military University Hospital of Oran (HMRUO) Algeria,characterize their antibiotic resistance profile, genetically detect blaTEM and blaCTX-M genes, and evaluate their biofilm formation capacity.Methodology: Different clinical specimens including blood, cerebrospinal fluids, urine and catheter, pus, perirectal abscess, and surgical wounds were collected from patients with suspected clinical infections in different units and departments of the hospital. The specimens were cultured on Blood, MacConkey and CLED agar (for urine only) plates and incubated aerobically for 24 hours at 37°C for preliminary identification of bacteria using conventional colony morphology, Gram stain reaction, and disk diffusion test for antibiotic susceptibility testing (AST). Confirmation of isolates, antibiogram, minimum inhibitory concentration (MIC) and detection of resistance phenotypes, were carried out by the automated Vitek 2 (BioMérieux) identification and susceptibility method. ESβL production was confirmed by the synergy and combination disk tests. ESβL genes were detected by conventional simplex PCR and biofilm formation was detected by the tissue culture plate (TCP) method.Results: A total of 630 patients’ clinical samples (one sample per patient) were processed. Klebsiella pneumoniae was isolated in 40 (6.3%) samples, and 15 of these (37.5%) produced ESβL. In the disk diffusion AST assay, all 40 K. pneumoniae isolates were resistant to ampicillin and ticarcillin while all 40 isolates were sensitive to cefoxitin, imipenem and ertapenem. KP-ESβL producing isolates were more frequently recovered from intensive care unit (33.3%) and from urine (46.7%) samples. Group 1 blaCTX-M genes were detected in 13 of the 15 (86.7%) KP-ESβL isolates, and 46.7% of these isolates were moderate biofilm producers.Conclusion: There is need for health departments to put in place preventative measures through regular surveillance of these resistant pathogens and initiating appropriate infection prevention and control strategies to limit their spread in Algerian hospitals and worldwide. Keywords: Klebsiella pneumoniae, ESβL, biofilm, PCR, antibacterial resistance French title: Klebsiella pneumoniae productrice de-lactamase spectre tendu dans l'hôpital universitaire militaire régional d'Oran, Algérie: résistance aux antibiotiques, formation de biofilm et détection desgènes blaCTX-M et blaTEM Contexte: Klebsiella pneumoniae est un pathogène bactérien communément associé aux infectionsnosocomiales et communautaires sévères, en particulier par l'acquisition de β-lactamases à spectre étendu(ESβL) et la capacité de formation de biofilm. Les objectifs de cette étude sont de déterminer la prévalence desisolats de K. pneumoniae producteurs de βLSE (KP-βLSE) au CHU d'Oran (HMRUO) Algérie, caractériser leurprofil de résistance aux antibiotiques, détecter génétiquement les gènes blaTEM et blaCTX-M, et évaluer leurcapacité de formation de biofilm.Méthodologie: Différents échantillons cliniques, y compris du sang, des liquides céphalo-rachidiens, de l'urinemictionnelle et du cathéter, du pus, des abcès périrectal et des plaies chirurgicales ont été prélevés despatients suspectés d'infections cliniques dans différentes unités et départements de l'hôpital. Les échantillonsont été cultivés sur des milieu de culture: deglose au sang, MacConkey et CLED (pour l'urine uniquement) etincubés en aérobie pendant 24heures à 37°C pour l'identification préliminaire des bactéries en utilisant lamorphologie conventionnelle des colonies, la coloration de Gram et le test de diffusion sur disque pour les testsde sensibilité aux antibiotiques (AST). La confirmation des isolats, l'antibiogramme, la concentration minimaleinhibitrice (CMI) et la détection des phénotypes de résistance ont été réalisés par la méthode automatiséed'identification et de sensibilité sur Vitek 2 (BioMérieux). La production de βLSE a été confirmée par les tests desynergie et de double disques. Les gènes de βLSE ont été détectés par PCR simplex conventionnelle et laformation de biofilm a été détectée par la méthode de la plaque de culture tissulaire (TCP).Résultats: Un total de 630 échantillons cliniques de patients (un échantillon par patient) ont été traités.Klebsiella pneumoniae a été isolé dans 40 échantillons (6,3%) et 15 d'entre eux (37,5%) ont produit des βLSE.Dans le test AST à diffusion sur disque, tous les 40 isolats de K. pneumoniae étaient résistants à l'ampicilline età la ticarcilline, tandis que les 40 isolats étaient sensibles à la céfoxitine, à l'imipénème et à l'ertapénème. Lesisolats producteurs de KP-βLSE ont été plus fréquemment récupérés dans les unités de soins intensifs (33,3%)et dans les échantillons d'urine (46,7%). Les gènes blaCTX-M du groupe 1 ont été détectés dans 13 des 15 isolatsde KP-βLSE (86,7%), et 46,7% de ces isolats étaient des producteurs de biofilm modérés.Conclusion: Il est nécessaire que les services de santé mettent en place des mesures préventives grâce à unesurveillance régulière de ces pathogènes résistants et à la mise en place de stratégies appropriées deprévention et de contrôle des infections pour limiter leur propagation dans les hôpitaux algériens et dans lemonde. Mots clés: Klebsiella pneumoniae, βLSE, biofilm, PCR, résistance antibactérienne
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- 2021
15. Gallic acid removal using fresh and calcined Ni-Al layered double hydroxides: Kinetics, equilibrium and response surface methodology (RSM) optimisation
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Nawal Taoufik, A. Elhalil, M. Achak, Noureddine Barka, M. Sadiq, M. Abdennouri, and Wafaa Boumya
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Health, Toxicology and Mutagenesis ,Kinetics ,Soil Science ,010501 environmental sciences ,engineering.material ,01 natural sciences ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,Human health ,Adsorption ,law ,Environmental Chemistry ,Calcination ,Gallic acid ,Response surface methodology ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Water Science and Technology ,010401 analytical chemistry ,Public Health, Environmental and Occupational Health ,Layered double hydroxides ,Pollution ,0104 chemical sciences ,chemistry ,Chemical engineering ,engineering - Abstract
Emerging contaminants have recently aroused attention because of their harmful impact on ecosystem and human health. Among those contaminants, standout gallic acid which is known for its high toxic...
- Published
- 2021
16. Sorption of methyl orange dye by dodecyl-sulfate intercalated Mg-Al layered double hydroxides
- Author
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Noureddine Barka, F. Kzaiber, M. El-Abboubi, A. Oussama, F.Z. Mahjoubi, and Nawal Taoufik
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chemistry.chemical_compound ,Aqueous solution ,Adsorption ,Chemistry ,Methyl orange ,Layered double hydroxides ,engineering ,Carbonate ,Sorption ,engineering.material ,Fourier transform infrared spectroscopy ,Nuclear chemistry ,Ion - Abstract
The aim of this study is to evaluate the Mg-Al layered double hydroxides (LDHs) containing dodecyl sulfate and carbonate ions in the removal of methyl orange (MO) from aqueous solution. The prepared materials (Mg-Al-CO3 and Mg-Al-Ds) were characterized by XRD, TEM and FT-IR methods. XRD patterns of the two materials show the interlayer type structure with (00 l) reflections. TEM analysis showed that the LDH samples have nanoscale structures. FTIR spectroscopy confirmed the presence of the desired anions in the interlayer space. The results of the adsorption experiences for MO were performed as a function of solution pH and contact time. The adsorption ability of MO onto Mg-Al-CO3 was practically influenced by pH solution. However, the adsorption capacity of MO by Mg-Al-Ds appears stable at the different values of solution pH. The maximum adsorption capacities were 185.06 and 97.5 mg/g for Mg-Al-Ds MB and Mg-Al-CO3, respectively. The adsorption tests recommend the use of organic LDHs as potential adsorbents for azo dye treatment in a wide range of pH.
- Published
- 2021
17. Box–Behnken design for understanding of adsorption behaviors of cationic and anionic dyes by activated carbon
- Author
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Mohamed Abdennouri, Wafaa Boumya, A. Machrouhi, M. Khnifira, M. Achak, Samir Qourzal, Noureddine Barka, and Hanane Tounsadi
- Subjects
Adsorption ,Chemical engineering ,Chemistry ,medicine ,Cationic polymerization ,Box–Behnken design ,Activated carbon ,medicine.drug - Published
- 2021
18. Ni-Fe-SDS and Ni-Fe-SO4 layered double hydroxides: Preparation, characterization and application in dyes removal
- Author
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Wafaa Boumya, A. Elhalil, M. Sadiq, Noureddine Barka, F.Z. Mahjoubi, and R. Elmoubarki
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010302 applied physics ,Materials science ,Scanning electron microscope ,Layered double hydroxides ,02 engineering and technology ,engineering.material ,021001 nanoscience & nanotechnology ,01 natural sciences ,chemistry.chemical_compound ,Crystallinity ,Adsorption ,chemistry ,0103 physical sciences ,Methyl orange ,engineering ,Fourier transform infrared spectroscopy ,Inductively coupled plasma ,0210 nano-technology ,Spectroscopy ,Nuclear chemistry - Abstract
This work involved the removal of textile dyes by synthesized Ni-Fe-SDS and Ni-Fe-SO4 layered double hydroxides (LDH). The adsorbent was synthesized using co-precipitation method at room temperature. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) and inductively coupled plasma (ICP). The obtained materials were used for the removal of methyl orange (MO) as a model anionic dye. Adsorption experiments were carried out under different solution pH and contact time. XRD analyses indicate low degree of crystallinity when using SDS as an intercalating anion in the LDH structure. Experimental results indicate rapid adsorption process with maximum adsorption capacity occurred in acidic medium. The maximum adsorption capacities were 105.36 and 82.45 mg/g, respectively for Ni-Fe-SDS and Ni-Fe-SO4 samples.
- Published
- 2021
19. Simultaneous H 2 Production and Bleaching via Solar Photoreforming of Model Dye‐polluted Wastewaters on Metal/Titania
- Author
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Mikel Imizcoz, Noureddine Barka, and Alberto V. Puga
- Subjects
Inorganic Chemistry ,Metal ,Materials science ,Hydrogen ,chemistry ,Chemical engineering ,visual_art ,Organic Chemistry ,Photocatalysis ,visual_art.visual_art_medium ,chemistry.chemical_element ,Physical and Theoretical Chemistry ,Catalysis - Published
- 2020
20. Chemical Composition and Anti-inflammatory Activity of Apium graveolens var. dulce Essential Oils from Senegal
- Author
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Michel Bakar Diop, Papa Seyni Cissokho, Ibrahima Ndiaye, Cheikhna Hamala Sanghare, Momar Talla Gueye, El Hadji Barka Ndiaye, Serigne Mbacké Diop, Abdoulaye Thiam, and Marie-Laure Fauconnier
- Subjects
Limonene ,Apiaceae ,biology ,Traditional medicine ,Chemistry ,medicine.drug_class ,Linoleic acid ,Apium graveolens ,General Medicine ,biology.organism_classification ,Anti-inflammatory ,law.invention ,Steam distillation ,chemistry.chemical_compound ,law ,medicine ,Food science ,Chemical composition ,Essential oil - Abstract
Apium graveolens var. dulce commonly known as celery, belongs to the Apiaceae family. It is used especially as a vegetable and medicinal plant. In Senegal, A. graveolens is used as a food condiment. The aim of this work is to study the chemical composition of A. graveolens stems (S), leaves (L) essential oils and anti-inflammatory activity. GC/FID and GC/MS analyzes carried out on essential oils obtained by steam distillation showed that oils from both stems and leaves were characterized by the same major constituents α-pinene, the prominent compound of oils represented 69.3 and 68.4% for stems (S) and leaves (L), respectively. It is followed by limonene (9.5 and 9.8%), α-phellandrene (5.5 and 5.9%) and β-pinene (4.8 and 4.3%). These compounds represented 89.1% (S) and 88.4% (L) of the total essential oils content. Anti-inflammatory activity was measured by the inhibition of 5-lipoxygenase (5-LOX) by A. graveolens essential oil anti-inflammatory assays revealed an IC50 of 29.5±2.0 µg/mL for A. graveolens oils and 23.7±0.5 µg/mL for quercetine used as a reference. This study showed that essential oils of A. graveolens was an important source of α-pinene who is probably responsible of its anti-inflammatory properties.
- Published
- 2020
21. Probiotic Bifidobacterium longum BB536 Viable Existence at Refrigeration Storage of Fermented Goat Milk Supplemented with Inulin and Different Cereal Bran (Sorghum, Barely and Millet)
- Author
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Saeed Abdullah Badahdah, Barka Mohammed Kabeir, Salma Elghali Mustafa, Salma Elzen Ibraheem, and Limia Hashim Mohamed
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chemistry.chemical_compound ,Probiotic ,Bifidobacterium longum ,Bran ,biology ,Chemistry ,law ,Inulin ,Fermentation ,Food science ,biology.organism_classification ,Sorghum ,law.invention - Published
- 2020
22. Behavior of TA6V Alloy at High Temperature in Air Plasma Conditions: Part 1—Oxidation
- Author
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Barka Lucile, Marianne Balat-Pichelin, Procédés, Matériaux et Energie Solaire (PROMES), and Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS)
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Materials science ,Alloy ,Oxide ,Thermodynamics ,02 engineering and technology ,Activation energy ,engineering.material ,Kinetic energy ,7. Clean energy ,01 natural sciences ,[SPI.MAT]Engineering Sciences [physics]/Materials ,symbols.namesake ,chemistry.chemical_compound ,0103 physical sciences ,General Materials Science ,Spallation ,ComputingMilieux_MISCELLANEOUS ,010302 applied physics ,Arrhenius equation ,[SPI.FLUID]Engineering Sciences [physics]/Reactive fluid environment ,Mechanical Engineering ,[SPI.PLASMA]Engineering Sciences [physics]/Plasmas ,Plasma ,Atmospheric temperature range ,021001 nanoscience & nanotechnology ,chemistry ,13. Climate action ,Mechanics of Materials ,[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic ,symbols ,engineering ,0210 nano-technology - Abstract
In order to mitigate debris in the low Earth orbit and to avoid dramatic collisions on Earth after atmospheric reentry at its end of life, the spacecraft mission has to take into account the influence of reentry on the spacecraft survivability. In this way, oxidation in air plasma conditions was studied on metallic alloys such as TA6V to obtain oxidation kinetic laws at high temperature and for short time duration (few minutes). Experimental results obtained for the oxidation of TA6V at high temperature and low pressure in air plasma conditions together with microstructural characterization using SEM and XRD are presented. For a constant holding time of oxidation of 300 s for two total air pressures (300 and 2000 Pa) in the temperature range 1300–1900 K, the mass variation versus reciprocal temperature follows an Arrhenius law that gives a same activation energy of around 62–64 kJ/mol showing the low influence of pressure. Then kinetics laws were obtained for a constant temperature of 1830–1840 K for 3 pressures (300, 2000 and 5000 Pa) in the short time interval 50–470 s. The oxidation of the TA6V alloy by atomic oxygen leads to the spallation of the oxide layers even for short duration, and thus, the space debris during its atmospheric reentry will lose very few mass and the debris will reach the Earth only partially destroyed. The mass loss was calculated, and finally, it was shown that it represents only 0.5‰ of the initial mass of the TA6V alloy.
- Published
- 2020
23. Zinc chloride activation of carob shells for heavy metals removal from water: statistical optimisation, characterisation and isotherm modelling
- Author
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M. Khnifira, H. Tounsadi, Rachid Elmoubarki, M. Farnane, Mohammed Barour, Samir Qourzal, A. Machrouhi, and Noureddine Barka
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Health, Toxicology and Mutagenesis ,010401 analytical chemistry ,Public Health, Environmental and Occupational Health ,Soil Science ,chemistry.chemical_element ,Heavy metals ,Zinc ,010501 environmental sciences ,01 natural sciences ,Pollution ,0104 chemical sciences ,Analytical Chemistry ,chemistry ,Environmental chemistry ,medicine ,Environmental Chemistry ,Sewage treatment ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Water Science and Technology ,Activated carbon ,medicine.drug - Abstract
This study is focused on the preparation of activated carbon based on carob shells waste by chemical activation with ZnCl2. The Box-Behnken experimental design with 17 experiences was used to optim...
- Published
- 2020
24. Molecular Marker Development and Gene Cloning for Diverse Disease Resistance in Pepper (Capsicum annuum L.): Current Status and Prospects
- Author
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Jundae Lee and Geleta Dugassa Barka
- Subjects
0106 biological sciences ,0301 basic medicine ,Genetics ,Candidate gene ,food and beverages ,Plant Science ,Genetically modified crops ,Biology ,Quantitative trait locus ,Plant disease resistance ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Gene mapping ,Genetic marker ,Molecular marker ,Gene ,010606 plant biology & botany ,Biotechnology - Abstract
The production of chili pepper (Capsicum annuum L.) is hindered by several biotic factors even though striding progresses were made in genetic improvement in the last two decades. Among the advancements were the fast-track genetic improvement of disease-resistant varieties by the use of marker-assisted selection (MAS) and the conventional breeding-based introgression of major resistance genes. Marker development, marker-based identification and fine mapping have revealed a large number of resistance genes, from which cloning of some candidate genes demonstrated the applicability and versatility of map-based cloning for disease resistance. In some of the recent fine mapping of disease resistance QTLs, closely linked DNA markers were identified, which in turn resulted in the rapid introgression of target gene(s) into breeding lines. Also, progresses were made on the characterization and map-based cloning of resistance genes conferring broad-spectrum resistance. As the number of identified and characterized resistance genes and the DNA markers linked to resistance genes are steadily generated, the development of multiple/durable resistance to major chili pepper diseases is accelerated by MAS. In the present review, the development of molecular markers, marker-based mapping of genes conferring resistance to ten major chili pepper diseases were discussed, focusing on the recent advancements in major and QTL-spanning resistance gene mapping. The review provides up-to-date insights into the development of DNA markers linked to disease resistance genes and the cloning of resistance genes, which are all so crucial in pepper breeding for disease resistance.
- Published
- 2020
25. Co-inoculation effect of Mesorhizobium ciceri and Pseudomonas fluorescens on physiological and biochemical responses of Kabuli chickpea (Cicer arietinum L.) during drought stress
- Author
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Tulu Degefu, Geleta Dugassa Barka, and Argen Adem Abdela
- Subjects
biology ,Physiology ,fungi ,Drought tolerance ,Mesorhizobium ,food and beverages ,Plant physiology ,Pseudomonas fluorescens ,Cell Biology ,Plant Science ,biology.organism_classification ,chemistry.chemical_compound ,Horticulture ,chemistry ,Chlorophyll ,parasitic diseases ,Shoot ,Genetics ,Mesorhizobium ciceri ,Ecology, Evolution, Behavior and Systematics ,Legume - Abstract
Chickpea (Cicer arietinum L.) is an important legume plant which is being affected by drought stress. Due to drought, chickpea production has been low in Ethiopia and other chickpea producing countries. Hence, the aim of this study was to reduce the impact of drought on chickpea plant by taking advantage of consortium effect of Mesorhizobium ciceri CP41 and Pseudomonas fluorescens Biotype G. Two chickpea varieties, Habru (drought tolerant) and Arerti (drought susceptible) were used to study the effect of the two bacterial consortia in moderating the effects of drought stress on some morphological, physiological and biochemical traits. The results showed that co-inoculation had significantly improved fresh and dry weight of leaves, root and shoot (p
- Published
- 2020
26. Theoretical and experimental study of the adsorption characteristics of Methylene Blue on titanium dioxide surface using DFT and Monte Carlo dynamic simulation
- Author
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A. Machrouhi, Noureddine Barka, F.Z. Mahjoubi, Wafaa Boumya, Hanane Tounsadi, M. Khnifira, M. Sadiq, Sana El Hamidi, Anass Mahsoune, and Mohamed Abdennouri
- Subjects
Dynamic simulation ,Surface (mathematics) ,chemistry.chemical_compound ,Materials science ,Adsorption ,chemistry ,Chemical engineering ,Titanium dioxide ,Monte Carlo method ,Methylene blue - Published
- 2020
27. Facile nitric acid activation of carob seeds for efficient recovery of heavy metals from water
- Author
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R. Elmoubarki, M. Farnane, M. Barour, Samir Qourzal, M. Khnifira, A. Machrouhi, Noureddine Barka, H. Tounsadi, and M. Abdennouri
- Subjects
chemistry.chemical_compound ,Chemistry ,Nitric acid ,Heavy metals ,Nuclear chemistry - Published
- 2020
28. Synthetic dyes adsorption and discoloration of a textile wastewater effluent by H3PO4 and H3BO3 activated Thapsia transtagana biomass
- Author
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Samir Qourzal, A. Machrouhi, Mohamed Abdennouri, M. Khnifira, Wafaa Boumya, A. Elhalil, Hanane Tounsadi, Noureddine Barka, and M. Sadiq
- Subjects
Textile ,Adsorption ,Wastewater ,business.industry ,Chemistry ,Biomass ,business ,Pulp and paper industry ,Effluent ,Thapsia transtagana - Published
- 2020
29. Microfiltración tangencial de jugo de mora (Rubus adenotrichus Schltdl.) endulzado con estevia (Stevia rebaudiana Bertoni)
- Author
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María Soledad Tapia González, César González Torrivilla, and Fabrice Vaillant Barka
- Subjects
Sucrose ,sweetener ,Bebida ,Microfiltration ,Ultrafiltration ,Pasteurization ,edulcorante ,formulation ,Conservation ,drink ,law.invention ,lcsh:Agriculture ,chemistry.chemical_compound ,law ,Stevioside ,Food science ,lcsh:Agriculture (General) ,membrane ,filtration ,biology ,conservation ,lcsh:S ,General Medicine ,conservación ,filtración ,biology.organism_classification ,Stevia ,lcsh:S1-972 ,formulación ,Stevia rebaudiana ,chemistry ,membrana ,Rebaudioside A - Abstract
The modern consumer values those components that can promote their well-being and help them reduce the risk of diseases the use of steviolglucosides as a sweetener in the formulation of foods is promising. The study evaluated the tangential microfiltration for clarification and conservation of blackberry juice sweetened with dried leaves of stevia. Two ceramic membranes were evaluated. The tests were performed at 35°C in concentration mode at a constant transmembrane pressure and tangential velocity. The formulation of the juice was defined through sensory evaluation tests. Volume, flow permeate, temperature, and pressure input and output were registered. The results of the variables analyzed in the process, as well as the physicochemical characterization of clarified juice, helped define the operating conditions for draw two formulations of microfiltrated juice, sweetened with stevia or sucrose, and thus evaluate the effectiveness of microfiltration as a conservation treatment. Aciduric microorganisms were quantified; molds and yeasts were carried out in the generated pasteurized samples stored at five different temperatures (5, 20, 30, 37, and 45°C). The recovery permeate juice obtained was 84.62%. The permeate Flow (Jp), exhibited higher values at 40L/hm2. Also, the concentration of stevioside and rebaudioside A in the retentate increased with advancing ultrafiltration. A significant variation in color, turbidity, and soluble solids, was observed, unlike the pH. Also, its chemical and microbiological stability is confirmed that allows you to preserve quality during 27 days of storage. RESUMEN Dada la importancia que el consumidor moderno le otorga a aquellos componentes que puedan promover su bienestar y ayudarle a reducir el riesgo de enfermedades, el uso de los esteviolglucósidos como edulcorante en la formulación de alimentos resulta prometedor. El presente estudio evaluó la microfiltración tangencial como tratamiento de clarificación y conservación de un jugo de mora endulzado con hojas deshidratadas de estevia. Se evaluaron dos membranas cerámicas. Los ensayos fueron efectuados a 35°C en modo concentración, a una presión transmembranaria y velocidad tangencial constante. La formulación del jugo fue definida a través de pruebas de evaluación sensorial. Se registró el volumen, flujo del permeado, la temperatura y la presión de entrada y salida. Los resultados de las variables analizadas y la caracterización fisicoquímica del jugo microfiltrado, permitieron definir las condiciones de operación para elaborar dos formulaciones, uno endulzado con estevia y otro con sacarosa y así evaluar la efectividad de la microfiltración. También se efectuaron recuentos de acidúricos, mohos y levaduras, en las muestras pastaurizadas almacenadas a cinco diferentes temperaturas (5, 20, 30, 37 y 45ºC). El estudio permitió obtener un 84,62% de recuperación del jugo permeado. El flujo de permeado (Jp), exhibió valores superiores a 40L/hm2. La concentración de esteviósido y rebaudiósido A en el retenido aumentó al avanzar la ultrafiltración. Se apreció, una variación de color, turbidez y sólidos solubles, a diferencia del pH. Asimismo se corroboró su estabilidad química y microbiológica al conservar su calidad durante 27 días de almacenamiento.
- Published
- 2019
30. Statistical optimization of activated carbon from Thapsia transtagana stems and dyes removal efficiency using central composite design
- Author
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S. El Hamidi, H. Alilou, M. Farnane, A. Machrouhi, M. Sadiq, Noureddine Barka, M. Abdennouri, and H. Tounsadi
- Subjects
Materials science ,Central composite design ,Materials Science (miscellaneous) ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Biomaterials ,chemistry.chemical_compound ,symbols.namesake ,lcsh:TA401-492 ,medicine ,Methyl orange ,Point of zero charge ,Methyl violet ,Langmuir adsorption model ,Sorption ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,Indigo carmine ,chemistry ,Ceramics and Composites ,symbols ,lcsh:Materials of engineering and construction. Mechanics of materials ,0210 nano-technology ,Nuclear chemistry ,Activated carbon ,medicine.drug - Abstract
This study focused on the preparation of activated carbons from Thapsia transtagana stems by boric acid activation and their evaluation for dyes removal. The central composite design and response surface methodology were used to optimize the preparation conditions. The effect of activation temperature, impregnation ratio and activation time on iodine number (IN), methylene blue index (MB index) and removal efficiencies of methyl violet (MV), methyl orange (MO) and indigo carmine (IC) were fully evaluated. The activated carbon samples prepared in optimal conditions were characterized by FTIR, XRD, SEM-EDX, Boehm's titration, and point of zero charge (pHPZC). The equilibrium data for dyes sorption onto optimum activated carbons were best fitted with Langmuir isotherm. Keywords: Thapsia transtagana stems, Dyes removal, Chemical activation, Central composite design
- Published
- 2019
31. Geotechnische Untersuchungen an modifizierten Stahlwerksschlacken
- Author
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Emanuel Birle, Elissavet Barka, and Derik Demond
- Subjects
Pollutant ,Cement ,chemistry.chemical_compound ,chemistry ,visual_art ,Metallurgy ,Earthworks ,Carbon dioxide ,visual_art.visual_art_medium ,Environmental science ,Slag ,Geotechnical Engineering and Engineering Geology - Published
- 2019
32. Assessing the Performance of Environmentally Friendly-Produced Zerovalent Iron Nanoparticles to Remove Pharmaceuticals from Water
- Author
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Nymphodora Papassiopi, Daniel Mamais, Iliana Panagou, Constantinos Noutsopoulos, Christiana Mystrioti, Elena Koumaki, Simos Malamis, Maria Kalli, and Evridiki Barka
- Subjects
Ketoprofen ,Naproxen ,Geography, Planning and Development ,Nanoparticle ,green tea extracts ,TJ807-830 ,Management, Monitoring, Policy and Law ,nano zerovalent iron ,TD194-195 ,Fenton process ,Renewable energy sources ,non-steroidal anti-inflammatory drugs (NSAIDs) ,medicine ,GE1-350 ,Ion-exchange resin ,Zerovalent iron ,Environmental effects of industries and plants ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Ibuprofen ,Environmentally friendly ,Environmental sciences ,Reagent ,contaminants of emerging concern ,green synthesis method ,medicine.drug ,Nuclear chemistry - Abstract
Nano zerovalent iron (nZVI), produced from green tea extracts, was incorporated in a cation exchange resin (R-nFe) to investigate its performance regarding the removal of four non-steroidal anti-inflammatory drugs (NSAIDs): ibuprofen (IBU), naproxen (NPX), ketoprofen (KTP) and diclofenac (DCF). The effect of contact time, NaCl pretreatment, pH, R-nFe dose, the role of the supporting material, the initial concentration of pollutants, and the combined effect of nZVI with oxidative reagents was assessed through a series of batch experiments. According to the results, the best removal efficiencies obtained for DCF and KTP were 86% and 73%, respectively, at 48 h of contact time with NaCl pretreated R-nFe at a dose of 15 g L−1 and a pH of 4. The maximum removal efficiency for NPX was 90% for a contact time of 60 min with PS 1 mM and a pH of 3, which was quite similar to the experiment with a greater contact time of 48 h without PS addition. The maximum IBU removal was 70%, this was reached at pH 3, with a contact time of 30 min and R-nFe 15 g L−1. To the authors’ best knowledge, this is the first study investigating the utilization of nZVI, produced from leaf extracts and incorporated into a cationic exchange resin, to remove NSAIDs from water.
- Published
- 2021
33. A review of knowledge on the mechanisms of action of the rare sugar d-tagatose against phytopathogenic oomycetes
- Author
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Andrea Nesler, Essaid Ait Barka, Aziz Aziz, Michele Perazzolli, Abdessalem Chahed, Ilaria Pertot, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Fondazione Edmund Mach - Edmund Mach Foundation [Italie] (FEM), Università degli Studi di Trento (UNITN), European Project: 722642,INTERFUTURE, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Settore BIO/04 - FISIOLOGIA VEGETALE ,Plant Science ,Horticulture ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,Genetics ,medicine ,tagatose ,rare sugar ,030304 developmental biology ,2. Zero hunger ,D-tagatose ,0303 health sciences ,030306 microbiology ,fungi ,food and beverages ,Rare sugar ,[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy ,Mechanism of action ,chemistry ,Action (philosophy) ,Biochemistry ,mechanism of action, microbial growth inhibition, phytopathogenic oomycetes, rare sugar, tagatose ,phytopathogenic oomycetes ,medicine.symptom ,Agronomy and Crop Science ,Tagatose ,mechanism of action ,microbial growth inhibition - Abstract
d-tagatose is a rare monosaccharide, naturally present at low concentrations in some fruits and dairy products.d-tagatose is "generally recognized as safe" and is used as a low-calorie sweetener in the food industry.It is able to inhibit the growth of numerous microorganisms, such as phytopathogenic oomycetes responsible for important crop diseases. Thanks to the negligible effects on human health and the environment,d-tagatose has been proposed as a sustainable product for crop protection. This review describes the current knowledge on modes of action ofd-tagatose against phytopathogenic oomycetes and its potential uses in agriculture.d-tagatose can negatively affect the growth of phytopathogenic oomycetes by inhibiting key enzymes of sugar metabolism, such as β-glucosidase inPhytophthora infestans, and fructokinase and phosphomannose isomerase inHyaloperonospora arabidopsidis. Moreover,d-tagatose affects sugar content, causes severe mitochondrial alterations, and inhibits respiration processes with the accumulation of reactive oxygen species inP.infestans, but not inP.cinnamomi. Differential effects ofd-tagatose are associated with a global gene downregulation inP.infestansand with an efficient transcriptional reprogramming of multiple metabolic processes inP.cinnamomi.d-tagatose displays possible species-specific effects inPhytophthoraspp. and nutritional properties on some plant-associated microorganisms. However, inhibitory effects are reversible andP.infestansgrowth can be restored in the absence ofd-tagatose. Further functional studies are discussed in this review, in order to promote the use ofd-tagatose for sustainable crop protection.
- Published
- 2021
34. Polyaniline/Nanomaterial Composites for the Removal of Heavy Metals by Adsorption: A Review
- Author
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M. Abdennouri, Noureddine Barka, Hind Hajjaoui, Amal Soufi, and Wafaa Boumya
- Subjects
Conductive polymer ,Technology ,Materials science ,Nanocomposite ,Aqueous solution ,Environmental remediation ,Science ,Nanotechnology ,Human decontamination ,Nanomaterials ,chemistry.chemical_compound ,Adsorption ,chemistry ,Polyaniline ,nanocomposites ,remediation ,Ceramics and Composites ,conductive polymer ,heavy metals removal ,Engineering (miscellaneous) - Abstract
Heavy metals represent one of the most important kinds of pollutants, causing serious threats to the ecological balance. Thus, their removal from aqueous solution is a major environmental concern worldwide. The process of adsorption—being very simple, economical, and effective—is widely applied for the decontamination of wastewaters from heavy metals. In this process, the adsorbent is the key factor affecting the performance; for this reason, significant efforts have been made to develop highly efficient and selective adsorbents with outstanding properties. This paper presents a detailed overview of the research on different methods of synthesis of nanocomposite materials based on the polymer polyaniline combined with nanomaterials, along with the influence of the synthesis method on their size, morphology, and properties. In addition, the study evaluates the adsorption efficiency of various developed nanocomposites for the adsorption of heavy metals from aqueous solution. From an economical and environmental point of view, the regeneration studies of the nanocomposites are also reported.
- Published
- 2021
35. Experimental and modeling studies of the removal of phenolic compounds from olive mill wastewater by adsorption on sugarcane bagasse
- Author
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Noureddine Barka, Younes Chhiti, Fatima Elayadi, Chakib El Adlouni, Wafaa Boumya, Mounia Achak, and Fatima Ezzahrae M'hamdi Alaoui
- Subjects
Olive mill wastewater ,Global and Planetary Change ,Langmuir ,Environmental Engineering ,High energy value ,Exothermic process ,Chemistry ,Kinetics ,Langmuir adsorption model ,Sugarcane bagasse ,Management, Monitoring, Policy and Law ,Pulp and paper industry ,Pollution ,Phenolic compounds ,Environmental sciences ,symbols.namesake ,Adsorption ,Wastewater ,symbols ,Freundlich equation ,GE1-350 ,Bagasse ,Waste Management and Disposal - Abstract
Olive mill wastewater (OMW) poses severe problems for aquatic and environmental systems, especially in the Mediterranean region, therefore, its treatment is of great interest. This study aimed the removal of phenolic compounds from OMW by sugarcane bagasse (SCB) as a low-cost adsorbent. Different parameters influencing the adsorption such as adsorbent dose, solution of pH, contact time and temperature were optimized. I was found that SCB can be used as an effective and low-cost adsorbent to remove phenolic compounds from OMW. Application of experimental design approach showed an optimum of 60% removal after 60 min at 60 °C and pH of 12. Langmuir and Freundlich isotherm models were used to describe the equilibrium date, while kinetics data were fitted to pseudo-first-order and pseudo-second-order kinetic models. The obtained results showed that the equilibrium data were best fitted to Freundlich model. The Langmuir model also gives good correlation and shows an adsorption capacity of 57.7 mg/g. kinetics data followed pseudo-second-order model instead of pseudo-second-order model. The thermodynamic parameters including ∆G°, ∆H° and ∆S° were also investigated and suggested an exothermic process. In addition, high energy value of SCB both before (13.15 MJ/kg) and after adsorption (15.58 MJ/kg) indicates its potential for use as an alternative energy source.
- Published
- 2021
36. Electrochemical sensors and biosensors for the determination of diclofenac in pharmaceutical, biological and water samples
- Author
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Haad Bessbousse, Nawal Taoufik, M. Achak, A. Elhalil, Noureddine Barka, and Wafaa Boumya
- Subjects
Chromatography ,Diclofenac ,QD71-142 ,Chemistry ,Potentiometric titration ,Context (language use) ,Electrochemical detection ,Modified electrodes ,stomatognathic diseases ,Detection limit ,Recovery ,Biological fluids ,medicine ,Biosensor ,Analytical chemistry ,medicine.drug - Abstract
Diclofenac is a non-steroidal anti-inflammatory drug of wide use around the world for the treatment of several diseases such as ankylosing spondylitis, acute muscle pain conditions, and osteoarthritis. Although in proper doses it does not display any side effects, an overdose of diclofenac can cause adverse effects in the human body. Moreover, the presence of diclofenac in ecosystems directly affects the health of living organisms, even if it is present at trace. Therefore, the development of analytical tools for diclofenac monitoring was needed. In this context, electrochemical sensing and biosensing methods have been commonly reported. In this review, a critical evaluation of the methods employed for diclofenac analysis is presented according to the following classifications: potentiometric sensors, sensing at non-modified solid electrodes, chemically modified electrodes and biosensors. Recent developments related to the use of electrochemical detection of diclofenac in pharmaceutical formulations, biological fluids and environmental samples were reported and discussed.
- Published
- 2021
37. The Differential Growth Inhibition of Phytophthora spp. Caused by the Rare Sugar Tagatose Is Associated With Species-Specific Metabolic and Transcriptional Changes
- Author
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Abdessalem Chahed, Valentina Lazazzara, Marco Moretto, Andrea Nesler, Paola Elisa Corneo, Essaid Ait Barka, Ilaria Pertot, Gerardo Puopolo, Michele Perazzolli, Fondazione Edmund Mach - Edmund Mach Foundation [Italie] (FEM), Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Trento (UNITN), European Project: 722642,INTERFUTURE, and Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,Alternative respiration ,Microbiology (medical) ,Phytophthora cinnamomi ,01 natural sciences ,[SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,transcriptomics ,transcriptional reprogramming ,Phytophthora spp ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,targeted metabolomics ,Sugar ,rare sugar ,030304 developmental biology ,Original Research ,2. Zero hunger ,0303 health sciences ,biology ,rare sugar, Phytophthora spp., transcriptomics, gene expression level, targeted metabolomics, transcriptional reprogramming ,biology.organism_classification ,Rare sugar ,QR1-502 ,[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy ,Citric acid cycle ,chemistry ,Biochemistry ,Phytophthora infestans ,Settore AGR/16 - MICROBIOLOGIA AGRARIA ,Phytophthora ,Tagatose ,gene expression level ,010606 plant biology & botany - Abstract
Tagatose is a rare sugar with no negative impacts on human health and selective inhibitory effects on plant-associated microorganisms. Tagatose inhibited mycelial growth and negatively affected mitochondrial processes in Phytophthora infestans, but not in Phytophthora cinnamomi. The aim of this study was to elucidate metabolic changes and transcriptional reprogramming activated by P. infestans and P. cinnamomi in response to tagatose, in order to clarify the differential inhibitory mechanisms of tagatose and the species-specific reactions to this rare sugar. Phytophthora infestans and P. cinnamomi activated distinct metabolic and transcriptional changes in response to the rare sugar. Tagatose negatively affected mycelial growth, sugar content and amino acid content in P. infestans with a severe transcriptional reprogramming that included the downregulation of genes involved in transport, sugar metabolism, signal transduction, and growth-related process. Conversely, tagatose incubation upregulated genes related to transport, energy metabolism, sugar metabolism and oxidative stress in P. cinnamomi with no negative effects on mycelial growth, sugar content and amino acid content. Differential inhibitory effects of tagatose on Phytophthora spp. were associated with an attempted reaction of P. infestans, which was not sufficient to attenuate the negative impacts of the rare sugar and with an efficient response of P. cinnamomi with the reprogramming of multiple metabolic processes, such as genes related to glucose transport, pentose metabolism, tricarboxylic acid cycle, reactive oxygen species detoxification, mitochondrial and alternative respiration processes. Knowledge on the differential response of Phytophthora spp. to tagatose represent a step forward in the understanding functional roles of rare sugars.
- Published
- 2021
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- View/download PDF
38. A sensitive chemiluminescence method for quantification of the oxidative burst in grapevine cells and rice roots
- Author
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Lisa Heyman, Essaid Ait Barka, Monica Höfte, Enrico Ferrarini, Lisa Sanchez, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Crops, Agricultural ,0106 biological sciences ,0301 basic medicine ,Luminescence ,[SDV]Life Sciences [q-bio] ,Plant Science ,Biology ,Plant Roots ,01 natural sciences ,law.invention ,03 medical and health sciences ,chemistry.chemical_compound ,law ,Plant Cells ,Genetics ,Extracellular ,Bioassay ,Vitis ,Inducer ,ComputingMilieux_MISCELLANEOUS ,Respiratory Burst ,Chemiluminescence ,2. Zero hunger ,chemistry.chemical_classification ,Reactive oxygen species ,Superoxide ,food and beverages ,Oryza ,General Medicine ,Elicitor ,Respiratory burst ,030104 developmental biology ,chemistry ,Biochemistry ,Evaluation Studies as Topic ,Reactive Oxygen Species ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
Roots are prominent plant-microbe interaction sites and of great biological relevance for many studies. The root response is of interest when searching for potential systemic resistance inducers. Screening of elicitors often focuses on the oxidative burst, the rapid and transient production of Reactive Oxygen Species (ROS). However, to our knowledge, no high-throughput, sensitive methods have been developed for the quantification of ROS released by roots. Here, we report on the development of an L-012-based chemiluminescence bioassay to quantitatively determine the oxidative burst following elicitation events in roots. Rice and grapevine were used as monocot and dicot models. We demonstrate that chitosan, a recognized elicitor in rice cells, was able to elicit ROS production in rice roots. Chitosan also triggered a strong oxidative burst in grapevine cell suspension cultures, while grapevine roots were not responsive. Although this method is broadly applicable, the L-012 probe requires careful consideration of solvents and plant species. Insufficient extracellular ROS, quenching, and the interference of solvents with the probe can undermine the assay sensitivity.
- Published
- 2021
39. Physicochemical characterization of natural sand from the south-east of Morocco and its potential use as sorbent for dyes removal
- Author
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M. Barour, R. Elmoubarki, H. Tounsadi, E.M. Lemdek, F.Z. Mahjoubi, M. Sadiq, Samir Qourzal, M. Abdennouri, A. Elhalil, and Noureddine Barka
- Subjects
Sorbent ,Chemistry ,Environmental chemistry ,South east ,Natural sand - Published
- 2019
40. Photocatalytic degradation of caffeine as a model pharmaceutical pollutant on Mg doped ZnO-Al2O3 heterostructure
- Author
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Samir Qourzal, F.Z. Mahjoubi, R. Elmoubarki, M. Sadiq, A. Elhalil, M. Farnane, A. Machrouhi, and Noureddine Barka
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Aqueous solution ,Materials science ,Diffuse reflectance infrared fourier transform ,Scanning electron microscope ,Materials Science (miscellaneous) ,Layered double hydroxides ,02 engineering and technology ,Management, Monitoring, Policy and Law ,engineering.material ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Pollution ,0104 chemical sciences ,Catalysis ,chemistry.chemical_compound ,chemistry ,Titanium dioxide ,Photocatalysis ,engineering ,Fourier transform infrared spectroscopy ,0210 nano-technology ,Waste Management and Disposal ,Water Science and Technology ,Nuclear chemistry - Abstract
In this work, a promising photocatalyst was prepared and investigated for the photocatalytic degradation of caffeine as a model pharmaceutical pollutant. Mg-doped ZnO-Al2O3 heterostructure with different content of Mg (1, 3 and 5 wt%) was prepared from Zn-Al-CO3 layered double hydroxides precursors using ceramic process. The synthesized catalysts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) coupled with energy dispersive X-ray analysis (EDX) and UV–vis diffuse reflectance spectroscopy (UV–vis DRS). The photocatalytic activity of the catalysts was evaluated for the degradation of caffeine in aqueous solutions under UV irradiation. Detailed experiments based on the effect of doping amount, irradiation time, photocatalyst dose, initial solution pH, caffeine concentration and reuse were performed and discussed in this study. The 1%Mg-ZnO-Al2O3 sample showed the highest photocatalytic activity with a degradation efficiency of 98.9% after 70 min of irradiation. This catalyst showed significantly higher degradation efficiency compared to undoped, pure ZnO and standard Degussa P-25 titanium dioxide. The photocatalyst showed good stability after three regeneration cycles.
- Published
- 2018
41. Biological Control of Pythium aphanidermatum, the Causal Agent of Tomato Root Rot by Two Streptomyces Root Symbionts
- Author
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Akbar Hosseinipour, Essaid Ait Barka, Ismail Saadoun, Roohollah Abdolshahi, Gholam Hosein Shahidi Bonjar, Mohadeseh Hassanisaadi, Shahid Bahonar University of Kerman, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), and University of Sharjah (UoS)
- Subjects
0106 biological sciences ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Pythium aphanidermatum ,Damping off ,[SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy ,Biology ,tomato ,Rhizobacteria ,01 natural sciences ,Streptomyces ,damping-off ,03 medical and health sciences ,chemistry.chemical_compound ,Streptomyces isolates ,root symbionts ,Root rot ,biocontrol ,16S rRNA ,Metalaxyl ,030304 developmental biology ,2. Zero hunger ,0303 health sciences ,food and beverages ,antagonist ,Agriculture ,biology.organism_classification ,3. Good health ,[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy ,Fungicide ,Horticulture ,chemistry ,PGPR ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
Damping-off caused by Pythium aphanidermatum, as one of the most infamous plant diseases, is considered as a significant disease that causes severe damages in greenhouse and field crops in a vast range of hosts especially vegetables. The application of chemical fungicides as a common method to control this disease poses negative side effects on humans and the environmental components. Actinobacteria, especially members of the genus Streptomyces, are fascinating biocontrol agents and plant growth-promoting rhizobacteria, which exhibit safer alternative managements to control this disease. The present study aims to explore for bioactives soil Streptomyces isolates that are able to control P. aphanidermatum, which is the causal agent of damping-off in tomato. Out of a total of 116 actinobacteria isolates collected from the soil, 53 have showed an antagonistic activity against P. aphanidermatum, as deduced through in vitro dual cultures. Based on in vitro Petri plate seedling-bioassays (IPSB), from the 53 tested isolates in dual cultures, two isolates coded as H2 and H3, considered as the most bioactive agents, were selected to assess their biocontrol performances against P. aphanidermatum in the Sandwich bed-mix technique in greenhouse experiments. In vivo greenhouse statistical studies were performed to compare seven treatments using completely randomized design experiments. Metalaxyl fungicide was applied as the chemical-control treatment. To evaluate biocontrol efficiencies of the two Streptomyces symbionts, disease incidence recorded throughout the course of experiment and criteria of number of leaves, length of the plants, plant fresh and dry weights, were measured at the end of experiment and analyzed statistically. The resulted disease incidences for all treatments indicated that the two Streptomyces strains had PGPR activity, and they were effective in decreasing disease incidence and improving plant performances regarding number of leaves, height, and plant fresh and dry weights. Based on the phylogenetic analysis of the partial sequences of the 16S rRNA gene, the strain H2 revealed a close relation to six Streptomyces species, namely S. badius, S. caviscabies, S. globisporus, S. parvus, S. sindenensis, and S. griseoplanus, with 99.9% similarity. The strain H3 also indicated a close relation of the same similarity to two species, namely S. flavogriseus and S. pratensis. In overall, collected data dictated that the two bioactive root symbiont Streptomyces strains effectively controlled the damping-off disease caused by P. aphanidermatum.
- Published
- 2021
42. Effect of aluminium incorporation on physicochemical properties and patent blue V photodegradation of magnesium phosphate materials
- Author
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M. Sadiq, M. Abdennouri, Mohammed Bensitel, Carole Lamonier, A. Elhalil, Noureddine Barka, Université Sultan Moulay Slimane (USMS ), Université Chouaib Doukkali (UCD), Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), and Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Materials science ,Coprecipitation ,chemistry.chemical_element ,physicochemical properties ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,law.invention ,Adsorption ,Aluminium ,law ,patent blue V photodegradation ,Specific surface area ,General Materials Science ,Fourier transform infrared spectroscopy ,Crystallization ,Photodegradation ,Magnesium phosphate ,aluminium incorporation ,[CHIM.CATA]Chemical Sciences/Catalysis ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,chemistry ,Mechanics of Materials ,0210 nano-technology ,Nuclear chemistry - Abstract
International audience; A series of magnesium phosphate samples modified by incorporation of aluminium, denoted MgAlP-x (where x indicates the Al molar ratio%; x = 0; 10; 20; 40%), have been prepared by coprecipitation method followed by characterization. Their structures have been investigated by using powder X-ray diffraction, the surface area by physisorption of nitrogen (BET), Fourier transform infrared (FTIR) were used to characterize the functional groups of solids and MAS–NMR spectroscopy. X-ray diffractograms for synthesized materials suggest the formation of mixed solids, such as Mg3(PO4)2, AlPO4 and Al2O3. Incorporation of aluminium into magnesium orthophosphate increased the temperature of crystallization, so that MgAlP-x systems retained amorphous up to 973 K. The specific surface area increases with aluminium content. 31P MAS–NMR spectra of calcined MgP shows a single peak at δ = +0.4 ppm and two overlapped signals for calcined MgAlP-x (x = 20 and 40%). But the dry solids present more than a single peak. The varying values of chemical shifts are due to the existence of different types of 31P structural configurations and oxygen environment. 27Al MAS-NMR spectra of sample exhibit two peaks, which were characteristic of the octahedral and tetrahedral environments of Al3+ ions. It was observed that the incorporation of Al in MgP material decreases the patent blue V (PB) photodegradation. The highest dye degradation was obtained over the MgP catalyst (without Al). No correlation between the adsorption before UV irradiation and the rate of photodegradation.
- Published
- 2021
43. Improving the Biocontrol Potential of Bacterial Antagonists with Salicylic Acid against Brown Rot Disease and Impact on Nectarine Fruits Quality
- Author
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Essaid Ait Barka, Rachid Lahlali, Rachida Ouaabou, Abdelali Blenzar, Chaimaa Letrib, Nadia Lyousfi, Said Ennahli, Zineb Belabess, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,Bacillus amyloliquefaciens ,Monilinia fructigena ,salicylic acid ,[SDV]Life Sciences [q-bio] ,Population ,biological control ,Bacillus amyloliquefaciens (SF14) ,Titratable acid ,Biology ,01 natural sciences ,lcsh:Agriculture ,03 medical and health sciences ,chemistry.chemical_compound ,In vivo ,Food science ,nectarines ,education ,030304 developmental biology ,0303 health sciences ,education.field_of_study ,Alcaligenes faecalis ,lcsh:S ,biology.organism_classification ,Alcaligenes faecalis (ACBC1) ,chemistry ,Agronomy and Crop Science ,Salicylic acid ,Bacteria ,010606 plant biology & botany - Abstract
The main objective of this study was to evaluate the ability of both antagonistic bacteria Bacillus amyloliquefaciens (SF14) and Alcaligenes faecalis (ACBC1) used in combination with salicylic acid (SA) to effectively control brown rot disease caused by Monilinia fructigena. Four concentrations of salicylic acid (0.5%, 2%, 3.5%, and 5%) were tested under in vitro and in vivo conditions. Furthermore, the impact of biological treatments on nectarine fruit parameters&rsquo, quality, in particular, weight loss, titratable acidity, and soluble solids content, was evaluated. Regardless of the bacterium, the results indicated that all combined treatments displayed a strong inhibitory effect on the mycelial growth of M. fructigena and disease severity. Interestingly, all SA concentrations significantly improved the biocontrol activity of each antagonist. The mycelial growth inhibition rate ranged from 9.79% to 88.02% with the highest reduction rate recorded for bacterial antagonists in combination with SA at both concentrations of 0.5% and 3.5%. The in vivo results confirmed the in vitro results with a disease severity varying from 0.00% to 51.91%. A significant biocontrol improvement was obtained with both antagonistic bacteria when used in combination with SA at concentrations of 0.5% and 2%. The lowest disease severity observed with ACBC1 compared with SF14 is likely due to a rapid adaptation and increase of antagonistic bacteria population in wounded sites. The impact of all biological treatments revealed moderate significant changes in the fruit quality parameters with weight loss for several treatments. These results suggest that the improved disease control of both antagonistic bacteria was more likely directly linked to both the inhibitory effects of SA on pathogen growth and induced fruit resistance.
- Published
- 2021
44. Combined DFT and MD simulation approach for the study of SO2 and CO2 adsorption on graphite (111) surface in aqueous medium
- Author
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Noureddine Barka, M. Khnifira, M. Sadiq, M. Abdennouri, L. Khamar, M.E. Belghiti, and A. Mahsoune
- Subjects
Materials science ,Binding energy ,Thermodynamics ,Electronegativity ,Molecular dynamics ,Chemistry ,Adsorption ,Greenhouse gases ,Molecular dynamics simulation ,Materials Chemistry ,Density functional theory ,Environmental Chemistry ,Molecule ,Molecular orbital ,Graphite ,Physical and Theoretical Chemistry ,QD1-999 - Abstract
In this study, density functional theory (DFT) calculations combined with molecular dynamics (MD) simulation have been used to understand the adsorption mechanism of sulfur dioxide (SO2) and carbon dioxide (CO2) on the graphite surface in aqueous medium, in view of analytical and environmental application. The global reactivity descriptors, such as the frontier molecular orbital energies (EHOMO and ELUMO), gap energy (ΔEgap), absolute hardness (η) and softens (σ), fraction of electrons transferred (ΔN) and electronegativity (χ) were evaluated. The dynamic descriptors calculated by the MD simulation such as the adsorption energy (Eads), total energy (Etot), deformation energy (Edef), rigid adsorption energy (RAE) and dEads/dNi were also calculated and discussed. The obtained results indicated strong electron acceptor ability and high adsorption energy, which can suggest good adsorption ability. The interaction energies (ΔEinteraction) were negative for both gases indicating strong attractive force between the molecules and the graphite surface. The highest values of the binding energy (ΔEbinding) indicate that the adsorption of each molecule is strong and stable, which is in agreement with the interaction energies. The values of EHOMO and Eads were negative, indicating that the complete chemical processes occur spontaneously. Furthermore, MD simulation was applied to investigate the most stable configuration of the molecules on the graphite (111) surface. The larger Eads and the smaller bond distances indicate that graphite exhibited higher sensitivity to interact with SO2 and CO2 molecules in an aqueous medium.
- Published
- 2021
45. Influence ofIndole-3-Acetic Acid treated Cowpea (Vignaunguiculata L. Walp) Seedson germination, plant development and leaf Chlorophyll Content
- Author
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Mshelmbula Barka Peter, Adama Michael, Solomon Peter Wante, Anoliefo Geoffrey Obidimbu, and Bello Suleman
- Subjects
Acetic acid ,chemistry.chemical_compound ,Horticulture ,Plant development ,Chlorophyll content ,chemistry ,Germination - Published
- 2021
46. Cobalt sulfide-reduced graphene oxide: An efficient catalyst for the degradation of rhodamine B and pentachlorophenol using peroxymonosulfate
- Author
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Baghdad Ouddane, Rabah Boukherroub, Alexandre Barras, Priyakshree Borthakur, Hervé Vezin, Lylia Amirache, Fatiha Barka-Bouaifel, Sabine Szunerits, Manash R. Das, Hania Ahouari, Université Abderrahmane Mira [Béjaïa], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), East Institute of Science and Technology, Jorhat, Academy of Scientific and Innovative Research [Ghaziabad, India] (AcSIR), Partenaires INRAE, Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 (LASIRE), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), The Centre National de la Recherche Scientifique (CNRS), the Hauts-de-France region and the University of Lille are acknowledged for financial support. L.A. thanks the French embassy in Algeria and the Algerian Ministry of Higher Education and Scientific Research (M.E.S.R.S) for a PROFAS B+2017 grant.This work was partly supported by the French Renatech network., Renatech Network, Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Centrale Lille Institut (CLIL), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université de Lille, CNRS, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Assam University, Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE], Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516, and Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
- Subjects
Thermogravimetric analysis ,Pentachlorophenol ,Rhodamine B ,chemistry.chemical_element ,02 engineering and technology ,010501 environmental sciences ,7. Clean energy ,01 natural sciences ,law.invention ,Catalysis ,chemistry.chemical_compound ,law ,Chemical Engineering (miscellaneous) ,Reduced graphene oxide ,Fourier transform infrared spectroscopy ,Electron paramagnetic resonance ,Waste Management and Disposal ,Cobalt sulfide ,PMS ,0105 earth and related environmental sciences ,[SDE.IE]Environmental Sciences/Environmental Engineering ,Process Chemistry and Technology ,[CHIM.CATA]Chemical Sciences/Catalysis ,021001 nanoscience & nanotechnology ,Pollution ,chemistry ,13. Climate action ,[SDU]Sciences of the Universe [physics] ,0210 nano-technology ,Cobalt ,Nuclear chemistry - Abstract
In this study, cobalt sulfide-reduced graphene oxide (CoS-rGO) nanocomposite was prepared by a solvothermal route and its structure, morphology and composition were assessed using various techniques, including transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) and thermogravimetric analysis (TGA). The catalytic performance of CoS-rGO was evaluated for activation of peroxymonosulfate (PMS) for the elimination of rhodamine B (RhB) and pentachlorophenol (PCP) in water. The effects of various operating parameters on the degradation efficiency were systematically studied. Under optimized operating conditions, the CoS-rGO/PMS system achieved fast (less than 10 min) elimination of RhB and PCP at room temperature, as evidenced by high-performance liquid chromatographic (HPLC) analysis. Electron Paramagnetic Resonance (EPR) studies along with scavenging experiments revealed that sulfate radicals ( S O 4 • − ) were the primary players in the catalytic process. Interestingly, the CoS-rGO catalyst was stable after 8 cycles without obvious activity loss. The results reported in the present study hold promise for potential application of CoS-rGO for PMS activation for the elimination of various organic pollutants.
- Published
- 2021
47. Pseudo-obstruction–inducing ACTG2R257C alters actin organization and function
- Author
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Changsong Yang, Tatyana Svitkina, Vasia Barka, Robert O. Heuckeroth, Sabine Schneider, and Sohaib Khalid Hashmi
- Subjects
0301 basic medicine ,Phalloidin ,macromolecular substances ,medicine.disease_cause ,Protein filament ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cell Movement ,Gene expression ,medicine ,Humans ,Actin ,Cells, Cultured ,Mutation ,Intestinal Pseudo-Obstruction ,Gastroenterology ,Muscle, Smooth ,General Medicine ,Cell Biology ,Subcellular localization ,Actin cytoskeleton ,Actins ,Cell biology ,Actin Cytoskeleton ,030104 developmental biology ,chemistry ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Ultrastructure ,Medicine ,Muscle ,Collagen ,Research Article ,Genetic diseases ,Muscle Contraction - Abstract
Actin γ 2, smooth muscle (ACTG2) R257C mutation is the most common genetic cause of visceral myopathy. Individuals with ACTG2 mutations endure prolonged hospitalizations and surgical interventions, become dependent on intravenous nutrition and bladder catheterization, and often die in childhood. Currently, we understand little about how ACTG2 mutations cause disease, and there are no mechanism-based treatments. Our goal was to characterize the effects of ACTG2R257C on actin organization and function in visceral smooth muscle cells. We overexpressed ACTG2WT or ACTG2R257C in primary human intestinal smooth muscle cells (HISMCs) and performed detailed quantitative analyses to examine effects of ACTG2R257C on (a) actin filament formation and subcellular localization, (b) actin-dependent HISMC functions, and (c) smooth muscle contractile gene expression. ACTG2R257C resulted in 41% fewer, 13% thinner, 33% shorter, and 40% less branched ACTG2 filament bundles compared with ACTG2WT. Curiously, total F-actin probed by phalloidin and a pan-actin antibody was unchanged between ACTG2WT- and ACTG2R257C-expressing HISMCs, as was ultrastructural F-actin organization. ACTG2R257C-expressing HISMCs contracted collagen gels similar to ACTG2WT-expressing HISMCs but spread 21% more and were 11% more migratory. In conclusion, ACTG2R257C profoundly affects ACTG2 filament bundle structure, without altering global actin cytoskeleton in HISMCs., A mutation associated with visceral myopathy profoundly disrupts actin gamma 2, smooth muscle (ACTG2) filament bundles without changing the global actin cytoskeleton in visceral smooth muscle cells.
- Published
- 2020
48. Induction of systemic resistance to Agrobacterium tumefaciens by endophytic bacteria in grapevine
- Author
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Behrouz Harighi, Essaid Ait Barka, Morahem Ashengroph, Susan Asghari, Aziz Aziz, Qassim Esmaeel, Christophe Clément, University of Kurdistan, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,0301 basic medicine ,[SDV]Life Sciences [q-bio] ,Plant Science ,Horticulture ,01 natural sciences ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Genetics ,Gall ,ComputingMilieux_MISCELLANEOUS ,Pathogenesis-related protein ,chemistry.chemical_classification ,biology ,Inoculation ,Phytoalexin ,Pseudomonas ,Agrobacterium tumefaciens ,biology.organism_classification ,030104 developmental biology ,chemistry ,Shoot ,Polyamine ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
Crown gall disease of grapevine, caused by Agrobacterium tumefaciens, often results in severe economic loss to grape production worldwide. This study demonstrated the ability of the endophytic bacteria Pseudomonas sp. Sn48 and Pantoea sp. Sa14 isolated from domesticated and wild grapevines to induce resistance in both above‐ and belowground tissues of grapevines infected with A. tumefaciens. Our results provide evidence that both strains can colonize roots and/or shoots. We showed that the strains Pseudomonas sp. Sn48 and Pantoea sp. Sa14 are capable of inducing stilbenic phytoalexin production in grapevine tissues and to further prime plantlets for enhanced phytoalexin production after A. tumefaciens inoculation. We also showed that in the majority of treatments, polyamine accumulation remained unchanged or slightly increased in plantlets treated with Pseudomonas sp. Sn48 and Pantoea sp. Sa14 compared with the control. Our findings indicated that the levels of polyamines remain unchanged or significantly decrease in plantlets treated with endophytic bacteria after A. tumefaciens challenge compared to the control and plantlets treated with individual endophytic bacterial strains. PR1, PR2, and PR4 gene expression levels of plantlets treated with Pseudomonas sp. Sn48 and Pantoea sp. Sa14 significantly increased after A. tumefaciens inoculation. The findings revealed the efficacy of the selected endophytic bacteria in triggering grapevine resistance against A. tumefaciens and the possible use of these strains as an alternative to chemical control methods in grapevine crown gall disease management.
- Published
- 2020
49. A regio- and stereoselectivity and molecular mechanism study on the addition reactions of morpholine and m-CPBA to 9α-hydroxyparthenolide using DFT calculations
- Author
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Wafaa Boumya, Mohamed Abdennouri, Sadiq Mhamed, A. Chekroun, Noureddine Barka, Ahmed Benharref, Mohamed Moumou, Redouan Hammal, Sanaa Elhamidi, and Asmaa Ben Naji
- Subjects
chemistry.chemical_classification ,Electron density ,Addition reaction ,Double bond ,Chemistry ,General Chemistry ,Medicinal chemistry ,lcsh:Chemistry ,chemistry.chemical_compound ,Stereospecificity ,lcsh:QD1-999 ,Morpholine ,Stereoselectivity ,Density functional theory ,Chemoselectivity - Abstract
The chemoselectivity and stereospecificity of the addition of the morpholine and the meta-chloroperoxybenzoic acid (m-CPBA) onto 9α-hydroxyparthenolide were studied using Density Functional Theory (DFT) calculations with the B3LYP/6-311+G(d,p) computational level within the Molecular Electron Density Theory (MEDT), to demonstrate the key role of the Global Electron Density Transfer (GEDT) and to examine the polar character of these reactions. This work is divided into two parts; the first part concerns the reaction between the morpholine and the 9α-hydroxyparthenolide. The second part deals with the epoxidation of the 9α-hydroxyparthenolide by m-CPBA followed by the addition of the morpholine to the major product resulting from the epoxidation step. The obtained results show that the reaction between the morpholine and the 9α-hydroxyparthenolide takes place on the double bond C3=C4. On the other hand, when 9α-hydroxyparthenolide is attacked by m-CPBA, the epoxidation reaction is carried out on the double bond C1=C2.
- Published
- 2020
50. Olive mill wastewater treatment using infiltration percolation in column followed by aerobic biological treatment
- Author
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Gomaa A. M. Ali, F.Z. Mahjoubi, Khalid Boutoial, A. Benamar, Abdelkhalek Oussama, Noureddine Barka, and Fouzia Kzaiber
- Subjects
Biochemical oxygen demand ,chemistry.chemical_classification ,Chemistry ,General Chemical Engineering ,Chemical oxygen demand ,General Engineering ,General Physics and Astronomy ,engineering.material ,Biodegradation ,Pulp and paper industry ,Wastewater ,medicine ,engineering ,General Earth and Planetary Sciences ,General Materials Science ,Sewage treatment ,Organic matter ,General Environmental Science ,Activated carbon ,medicine.drug ,Lime - Abstract
This work aims to treat olive mill wastewater (OMWW) by infiltration percolation in different columns containing filtration sand, granular activated carbon, and lime, and thus followed by biological treatment using the soil microorganisms to the treatment that will give the best results. The optimum removal efficiency was obtained using granular activated carbon column mixed with 15% of lime with percentages removal of 39.33, 60.28, 53.17 and 88.74% for biological oxygen demand (BOD5), chemical oxygen demand (COD), polyphenols and suspended matter respectively. Aerobic treatment 30 days of OMWW obtained from the column of activated carbon mixed with 15% of lime, neutralized and diluted 15 times with distilled water was investigated. The results showed a significant percentage reduction of 79.78, 69.43, and 60.67%, respectively, for BOD5, COD, and polyphenols. The global removal percentages after the two successive treatments were 87.86% for COD, 87.39% for BOD5 and 81.59% for polyphenols compounds. Fourier Transformed Infrared spectroscopy analysis of crude OMWW, infiltrated percolated and biologically treated, showed a difference between the spectra that manifests by the reduction of the spectrum intensity bands after each treatment stage, which confirmed the analytical results. Soil microorganisms have shown their biodegradation efficiency of organic matter and polyphenols of these much-polluted effluents under aerobic conditions. The results indicated that the pretreatment of raw olive mill wastewater could be realized before biological treatment to obtain a satisfactory reduction.
- Published
- 2020
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