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Precise control of DNA‐circuit functions facilitates the construction of complex DNA networks to perform sophisticated functions. Inspired by optochemical genetics with high precision for controlling and studying neural networks by photoregulation of membrane receptors, we herein report optochemically controlled DNA switching circuits for regulating computation functions. The DNA‐switching circuits involve a CG–C+ triplex‐based DNA switch that undergoes structural transition from triplex to duplex states after exposure to UV irradiation due to proton transfer, showing optochemical control. We demonstrate that the DNA‐switching circuits enable the regulation of computation functions by optochemical control of the state of the DNA switch, including multiple logic computations and probabilistic computation. We hope this work will broaden DNA‐circuit functions and facilitate the construction of complex DNA networks. [ABSTRACT FROM AUTHOR]

We have developed a set of DNA circuits that execute during gel electrophoresis to yield immobile, fluorescent features in the gel. The parallel execution of orthogonal circuits led to the simultaneous production of different fluorescent lines at different positions in the gel. The positions of the lines could be rationally manipulated by changing the mobilities of the reactants. The ability to program at the nanoscale so as to produce patterns at the macroscale is a step towards programmable, synthetic chemical systems for generating defined spatiotemporal patterns.

We have developed a theoretical framework for developing patterns in multiple dimensions using controllable diffusion and designed reactions implemented in DNA. This includes so-called strand displacement reactions in which one single-stranded DNA hybridizes to a hemi-duplex DNA and displaces another single-stranded DNA, reversibly or irreversibly. These reactions can be designed to proceed with designed rate and molecular specificity. By also controlling diffusion by partial complementarity to a stationary, cross-linked DNA, we can generate predictable patterns. We demonstrate this with several simulations showing deterministic, predictable shapes in space. [ABSTRACT FROM AUTHOR]

We have developed a set of DNA circuits that execute during gel electrophoresis to yield immobile, fluorescent features in the gel. The parallel execution of orthogonal circuits led to the simultaneous production of different fluorescent lines at different positions in the gel. The positions of the lines could be rationally manipulated by changing the mobilities of the reactants. The ability to program at the nanoscale so as to produce patterns at the macroscale is a step towards programmable, synthetic chemical systems for generating defined spatiotemporal patterns [ABSTRACT FROM AUTHOR]

Titelbild: Optochemical Control of DNA-Switching Circuits for Logic and Probabilistic Computation (Angew. Keywords: chemical reaction networks; DNA circuits; DNA switches; logic computations; probabilistic computations EN chemical reaction networks DNA circuits DNA switches logic computations probabilistic computations 3353 3353 1 02/10/21 20210215 NES 210215 B Ein molekularer Computer b aus optochemischen DNA-Schaltkreisen wurde entwickelt, der digitale Rechenoperationen einschließlich Boolescher Logik (dargestellt durch 0 und 1) und Wahrscheinlichkeitsrechnungen (dargestellt durch die Würfel), ausführen kann. Chemical reaction networks, DNA circuits, DNA switches, logic computations, probabilistic computations. [Extracted from the article]