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Phytoremediation, assisted with different amendments, is receiving much attention around the world due to its high efficiency, low cost and ease of handling. The current study was constructed to determine the combined performance of the citric acid (CA) and 5-aminolevulinic acid (5-AA) to enhance chromium (Cr) extraction through sunflower. Healthy seeds of sunflower were grownup in Cr contaminated soil (0, 5, 10 & 20 mgkg-1) and externally provided with CA (0, 2.5 &5 mM) and 5-AA (0, 10 & 20 mg L-1) at juvenile stage. The results showed a significant reduction in sunflower agronomic traits and biomass under Cr stress. In response to Cr stress, higher production of the reactive oxygen species (ROS) significantly reduced soluble proteins, photosynthetic pigments and activities of anti-oxidant enzymes. The combined addition of CA and 5-AA considerably mitigated the Cr-induced toxic effects on sunflower. Citric acid (CA) and 5-AA application enhanced plants' agronomic and physiological attributes by lowering electrolyte leakage and ROS production. Furthermore, the introduction of 5-AA and CA significantly up-regulated the actions of antioxidants enzymes in leaves and root of sunflower. With increasing soil Cr concentration, more Cr uptake and accumulation was observed which was further boosted with mutual applications of CA and 5-AA. Results of our work summarize that, CA and 5-AA combined application can effectively mitigate Cr stress and enhance Cr extraction capability of sunflower. [ABSTRACT FROM AUTHOR]

Phytoremediation is an important technique to remove heavy metals from contaminated soils due to its efficiency and cost-effectiveness. The present study was conducted to assess the synergistic role of 5-aminolevulinic acid (ALA) and citric acid (CA) in improving the phyto-extraction of chromium (Cr) by sunflower. Sunflower plants were grown in soil, spiked with different concentrations of Cr (0, 5, 10, 20mg kg-1). Various concentrations of 5-ALA (0, 10, 20mg L-1) and CA (0, 2.5, 5mM) were applied exogenously at juvenile stage. A significant decrease was observed in biomass and agronomic traits of sunflower under Cr stress alone. Further, Cr toxicity significantly decreased the plant growth, soluble proteins and photosynthetic pigments. However, exogenously applied ALA and CA significantly improved the plants' physiological as well as agronomic attributes by lowering the production of reactive oxygen species and reducing electrolyte leakage. Moreover, Cr uptake was increased with increasing concentration of Cr in spiked soil, which was further enhanced by combined application of ALA and CA. [ABSTRACT FROM AUTHOR]

Soil and water contamination from heavy metals and metalloids is one of the most discussed and burning global issues due to its potential to cause the scarcity of healthy food and safe water. The scientific community is proposing a range of lab and field based physical, chemical and biological solutions to remedy metals and metalloids contaminated soils and water. The present study finds out a possibility of Chromium (Cr) extraction by sunflower from spiked soil under chelating role of citric acid (CA). The sunflower plants were grown under different concentrations of Cr (0, 5, 10 & 20 mg kg −1 ) and CA (0, 2.5 & 5 mM). Growth, biomass, gas exchange, photosynthesis, electrolyte leakage (EL), reactive oxygen species (ROS; malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ) and the activities of antioxidant enzymes such as, superoxide dismutase (SOD), guaiacole values peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT) were measured. The results depicted a clear decline in plant height, root length, leaf area, number of leaves and flowers per plant along with fresh and dry biomass of all parts of plant with increasing concentration of Cr in soil. Similar reduction was observed in chlorophyll a and b, total chlorophyll, carotenoids, soluble protein, gas exchange attributes and SPAD. The increasing concentration of Cr also enhanced the Cr uptake and accumulation in plant roots, stem and leaves along with the production of ROS and EL. The activities of antioxidant enzymes increased with increasing Cr concentration from 0 to 10 mg, but decreased at 20 mg kg −1 soil. The CA application significantly alleviated Cr-induced inhibition of plant growth, biomass, photosynthesis, gas exchange, soluble proteins and SPAD value. Presence of CA also enhanced the activities of all antioxidant enzymes and reduced the production of ROS and EL. The chelating potential of CA increased the concentration and accumulation of Cr in plant roots, stem and leaves. It is concluded that the sunflower can be a potential candidate for the remediation of Cr under CA treatment, while the possibility may vary with genotype, Cr level and CA concentration. [ABSTRACT FROM AUTHOR]

Chelate-assisted phytoextraction by high biomass producing macrophyte plant Typha latifolia L. commonly known as cattail, is gaining much attention worldwide. The present study investigated the effects of Lead (Pb) and Mercury (Hg) on physiology and biochemistry of plant, Pb and Hg uptake in T. latifolia with and without citric acid (CA) amendment. The uniform seedlings of T. latifolia were treated with various concentrations in the hydroponics as: Pb and Hg (1, 2.5, 5 mM) each alone and/or with CA (5 mM). After four weeks of treatments, the results revealed that Pb and Hg significantly reduced the plant agronomic traits as compare to non-treated plants. The addition of CA improved the plant physiology and enhanced the antioxidant enzymes activities to overcome Pb and Hg induced oxidative damage and electrolyte leakage. Our results depicted that Pb and Hg uptake and accumulation by T. latifolia was dose depend whereas, the addition of CA further increased the concentration and accumulation of Pb and Hg by up to 22 & 35% Pb and 72 & 40% Hg in roots, 25 & 26% Pb and 85 & 60% Hg in stems and 22 & 15 Pb and 100 & 58% Hg in leaves respectively compared to Pb and Hg treated only plants. On other hand, the root-shoot translocation factor was ≥1 and bioconcentration factor was also ≥2 for both Pb & Hg. The results also revealed that T. latifolia showed greater tolerance towards Hg and accumulated higher Hg in all parts compared with Pb. Image 1 • Lead and Hg ecotoxicity altered physiological and biochemical functions. • Citric acid improved antioxidant defense system and photosynthetic pigments. • Citric acid alleviates Pb and Hg stress and enhanced plant growth and biomass. • Citric acid enhanced uptake and accumulation of Pb and Hg in Typha latifolia. [ABSTRACT FROM AUTHOR]