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Charcoal Rot (CR) caused by Macrophomina Phaseolina has adverse effect on sesame crop in Pakistan. Use of host plant resistance is the cheapest and effective disease management strategy. In this research 52 local sesame genotypes were used to evaluate resistance against Charcoal rot disease in two consecutive summer seasons i.e. 2017 and 2018, in a sick field under natural conditions. The results showed that there was notable difference in disease infection percentage (DI%) of the varieties tested in two years. Furthermore, the results indicated that DI% for genotypes tested in 2017 ranged from 06.97% to 50.82%, whereas, DI% for genotypes tested in 2018 remained between 4.91% and 52.63%. In both seasons only 3 sesame lines L-7, ML-6-8/12 and L-100 were classified as a resistant (R) and they exhibited lower means of DI% 8.21 %, 7.84 %, 6.97% andDI% 8.19, 6.15, 4.91 during 2017 and 2018 respectively. On the other hand, 12 lines name Black Til, TH-6, No-7/12, ML-6-8-12, Cluster, 70004, L-41, 20011, 50022, L-24, No-6/12, and TS-3were classified as a moderately resistant (MR).Twenty three lines were grouped into moderately susceptible (MS)class and the susceptible (S) group contains 8 genotypes 95006-2, 97005, 97001, 97002, 92001-52-9, 96008, 16003 and Shan in both seasons but three lines such as 93003, 93005-3-4, and No-56 were moderately resistant MR in first season and moderately susceptible in second season. Similarly the genotypes 9600- 4-1 and Agara were moderately susceptible (MS) in first season and susceptible (S) in second season. Line N-10 was highly susceptible to CR with maximum DI% 50.8 and 52.63 in both seasons. [ABSTRACT FROM AUTHOR]