1. The physicochemical and biological characterization of a 24-month-stored nanocomplex based on gold nanoparticles conjugated with cetuximab demonstrated long-term stability, EGFR affinity and cancer cell death due to apoptosis.
- Author
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Andrade LM, Martins EMN, Versiani AF, Reis DS, da Fonseca FG, Souza IP, Paniago RM, Pereira-Maia E, and Ladeira LO
- Subjects
- Antineoplastic Agents chemistry, Antineoplastic Agents metabolism, Cell Line, Tumor, Cetuximab immunology, Cetuximab pharmacology, Drug Carriers chemistry, Drug Stability, ErbB Receptors chemistry, ErbB Receptors immunology, ErbB Receptors metabolism, Humans, Neoplasms metabolism, Neoplasms pathology, Antineoplastic Agents pharmacology, Apoptosis drug effects, Cetuximab chemistry, Gold chemistry, Metal Nanoparticles chemistry, Nanostructures chemistry
- Abstract
Nanotechnology is one of the most promising tools for future diagnosis and therapy. Thus, we have produced gold nanoparticles coated with cetuximab at a dose-range from 5 μg up to 200 μg, and prolonged stable nanocomplexes were obtained. The nanocomplexes were characterized by UV-Vis, zeta potential, TEM, fluorometry, infrared regions, XPS and atomic absorption spectrometry. For biological characterization the A431 cell line was used. Cellular uptake, target affinity and cell death were assessed using ICP-OES, immunocytochemistry and flow cytometry, respectively. The immobilization of cetuximab on the AuNPs surfaces was confirmed. The nanocomplex with 24 months of manufacturing promoted efficient EGFR binding and induced tumour cell death due to apoptosis. Significant (p < 0.05) cell death was achieved using relatively low cetuximab concentration for AuNPs coating compared to the antibody alone. Therefore, our results provided robust physicochemical and biological characterization data corroborating the cetuximab-bioconjugate AuNPs as a feasible nanocomplex for biomedical applications., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2020
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