Search

Your search keyword '"Altay Koçak, Aylin"' showing total 2 results
Start Over Author "Altay Koçak, Aylin" Topic cerebrospinal fluid
2 results on '"Altay Koçak, Aylin"'

1. Atipik menenjit/ensefalit tanısı almış hastalarda, enterovirüsler, herpesvirüsler (1, 2, 6, 7), flavivirüsler ve flebovirüslerin polimeraz zincir reaksiyonu yöntemi ile araştırılması

Author

Altay Koçak, Aylin, Bozdayı, Gülendam, Ergünay, Koray, and Mikrobiyoloji Anabilim Dalı

Subjects
Moleküler Tıp, Meninges, Cerebrospinal fluid, Mikrobiyoloji, Flaviviridae, Viruses, Molecular Medicine, Encephalitis, Microbiology, Herpesviridae, Enteroviruses, Polymerase chain reaction
Abstract

Bu çalışmada, atipik menenjit/ensefalitli hastalarda, PZR yöntemi ile herpesvirüsler (tip 1,2,6,7), enterovirüsler (EV), flavivirüsler ve flebovirüslerin araştırılması ve epidemiyolojik veri elde edilmesi amaçlanmıştır. Ankara'da iki farklı hastanenin Çocuk Enfeksiyon Bölümü'ne Ekim 2011-Aralık 2015 tarihleri arasında nörolojik şikayetlerle başvuran, 0-18 yaş arasındaki 100 hastadan beyin omurilik sıvısı (BOS) örnekleri toplanmıştır. Nükleik asit ekstraksiyonu ve cDNA sentezi için ticari kitler kullanılmıştır. HSV1/2 gerçek zamanlı PZR (LightCycler® HSV1/2 QualKit, Roche, Almanya); enterovirüsler, flavi- ve flebovirüsler geleneksel PZR yöntemi ile araştırılmış ve ayrıca çeşitli menenjit etkenlerini saptayabilen gerçek zamanlı multipleks PZR yöntemi (MeningoFinder® 2SMART, Hollanda) kullanılmıştır. Çalışılan 100 örneğin 7'si (%7) viral etkenler (2 HSV1, 4 HHV6, 1 EV) açısından pozitif; diğer etkenler negatif bulunmuştur. HHV6 pozitif saptanan 4 hastanın 2'si 1-6 aylık, 1'i 13-36 aylık ve diğeri ise 4-6 yaş grubundadır. HHV6 pozitif hastaların 3'ü menenjit, 1'i viral ensefalit; HSV1 pozitif hastalar viral ensefalit; EV pozitif hasta ise menenjit ön tanısı almıştır. Merkezi sinir sistemi enfeksiyonlarında çok sayıda virüs etken olabilmektedir ve etkeni saptanabilen olgu sayısı düşüktür. HSV1 viral ensefalit etkenleri arasında başlarda yer alsa da, HHV6 ile nadir karşılaşılmaktadır. Ayrıca HHV6'nın etken olma durumu, latent bir virüs olması nedeniyle her olguda kesin değildir. Yine de bu etkenlerin, MSS enfeksiyonlarında rolü olabileceği için, tanı algoritmalarında değerlendirilmesi gerekmektedir. The aim of this study was to investigate the impact of enteroviruses (EV), herpesviruses (types 1,2,6,7), flaviviruses and phleboviruses in patients with atypical meningitis/encephalitis and to obtain epidemiological data. One hundred cerebrospinal fluid (CSF) specimens were collected from 100 patients (age:0-18) that presented with neurological complaints at Paediatric Infections Clinics of two hospitals in Ankara from October 2011 to December 2015. Commercial kits were used for nucleic acid extraction and cDNA synthesis. A commercial real time PCR assay (LightCycler® HSV1/2 QualKit, Roche, Germany) was employed for HSV1/2 detection; enteroviruses, flaviviruses, phleboviruses were investigated by in house PCR methods. Also a commercial real time multiplex PCR method (MeningoFinder® 2SMART, The Netherlands), which can detect various meningitis agents, was used. A total of 7 specimens (7%) were positive for viral agents (2 HSV1, 4 HHV6, 1 EV) and other agents tested were negative. Two of HHV6 positive patients were 1-6 months old, 1 was 13-36 months old and the other was 4-6 years old. HHV6 was detected in 3 patients with meningitis, in 1 patient with viral encephalitis, HSV1 was detected in 2 patients with viral encephalitis and EV positive patient had meningitis. Many viral agents can cause central nervous infections and detectable number of cases is low. Although HSV1 is the most common agent in viral encephalitis cases, HHV6 is a rare agent. Thus, these viral agents that might have a role in CNS infections, should be considered in diagnostic algorithms. 94

Published
2017

2. Çeşitli nörolojik hastalıklarda herpes simpleks virüs tip 1 pozitifliği.

Author

ALTAY KOÇAK, Aylin, ÇOLAK, Meryem, İRKEÇ, Ceyla, SERDAROĞLU, Ayşe, AKTAŞ-TAPISIZ, Anıl, TEZER, Hasan, AVCIKÜÇÜK, Havva, FIDAN, Işıl, ÖZKAN, Seçil, and BOZDAYI, Gülendam

Subjects
*NEUROLOGICAL disorders, *GUILLAIN-Barre syndrome, *DISEASE progression, *HERPES simplex, *MOLECULAR microbiology, *CEREBROSPINAL fluid
Abstract

Objective: Herpesviruses are etiology of various neurological disorders causing morbidity and also mortality in both immunocompetent and immunocompromized children and adults. Viruses play an important role in etiology of encephalitis but their role is still unclear in neurologic diseases such as multiple sclerosis or Guillain-Barre syndrome. Studies revealed that only in few cases, viruses were isolated. But viruses are thought to have a role in triggering the diseases process or progression of the diseases in pathogenesis of neurological disorders, especially in multiple sclerosis. The aim of this study was detection of HSV-1 DNA by Real Time PCR in patients applying to our hospital with neurological symptoms and review of clinical picture in positive patients. Methods: Totally 150 patients whose samples sent to Molecular Microbiology Laboratory between 1 April 2014-31 January 2016 were included in this study. DNAs were extracted by spin-column method (High Pure Viral Nucleic Acid Kit, Roche, Germany). Amplification was done by Real time PCR method (LightCycler®HSV1/2 QualKit, Roche, Germany) in LightCycler 2.0 (Roche, Germany) device and the results were evaluated qualitatively. HSV-1 IgM and HSV-1 IgG antibody titres of the patients were studied by ELISA (DIA. PRO, Milan, Italy). Results: Totally 164 samples were composed of 82.9% (136/164) CSF and 17.1% (28/164) blood. HSV-1 DNA was found positive for 4.8% (8/164) of samples while all samples were negative for HSV-2 DNA. HSV-1 IgM was positive for 6.2% (2/32) and HSV-1 IgG was positive for 57.9% (11/19) of patients. There was no statistically significant difference between HSV-1 DNA positivity in blood and CSF samples of patients and their ages and gender. Three (50%) of positive patients were associated with Herpes simplex encephalitis, the other positive patients had multiple sclerosis, Wilson disease and Guillain Barre syndromes. Conclusion: Real time PCR is a reliable and sensitive method for giving results in short time to patients with neurological symptoms and detecting low positive results. Thus early treatment prevents sequels. There are studies demonstrating the relation of HSV-1 with neurological disorders especially multiple sclerosis and Guillain Barre syndrome. Therefore, we consider that testing HSV-1 can be sensible for the patients with variable neurological disorders. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results