1. No replication of direct neuronal activity-related (DIANA) fMRI in anesthetized mice.
- Author
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Choi SH, Im GH, Choi S, Yu X, Bandettini PA, Menon RS, and Kim SG
- Subjects
- Mice, Animals, Neurons physiology, Thalamus physiology, Vibrissae physiology, Oxygen, Brain Mapping methods, Magnetic Resonance Imaging methods, Cerebral Cortex
- Abstract
Direct imaging of neuronal activity (DIANA) by functional magnetic resonance imaging (fMRI) could be a revolutionary approach for advancing systems neuroscience research. To independently replicate this observation, we performed fMRI experiments in anesthetized mice. The blood oxygenation level-dependent (BOLD) response to whisker stimulation was reliably detected in the primary barrel cortex before and after DIANA experiments; however, no DIANA-like fMRI peak was observed in individual animals' data with the 50 to 300 trials. Extensively averaged data involving 1050 trials in six mice showed a flat baseline and no detectable neuronal activity-like fMRI peak. However, spurious, nonreplicable peaks were found when using a small number of trials, and artifactual peaks were detected when some outlier-like trials were excluded. Further, no detectable DIANA peak was observed in the BOLD-responding thalamus from the selected trials with the neuronal activity-like reference function in the barrel cortex. Thus, we were unable to replicate the previously reported results without data preselection.
- Published
- 2024
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