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3 results on '"Aragones, Julian"'

1. Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1.

Author

Ruiz de Almodovar C, Coulon C, Salin PA, Knevels E, Chounlamountri N, Poesen K, Hermans K, Lambrechts D, Van Geyte K, Dhondt J, Dresselaers T, Renaud J, Aragones J, Zacchigna S, Geudens I, Gall D, Stroobants S, Mutin M, Dassonville K, Storkebaum E, Jordan BF, Eriksson U, Moons L, D'Hooge R, Haigh JJ, Belin MF, Schiffmann S, Van Hecke P, Gallez B, Vinckier S, Chédotal A, Honnorat J, Thomasset N, Carmeliet P, and Meissirel C

Subjects
Animals, Apoptosis physiology, Blotting, Western, Cells, Cultured, Cerebellum cytology, Enzyme-Linked Immunosorbent Assay, Growth Cones metabolism, HEK293 Cells, Humans, Immunohistochemistry, Mice, Mice, Transgenic, Microscopy, Confocal, Neurons cytology, Protein Isoforms metabolism, Reverse Transcriptase Polymerase Chain Reaction, Vascular Endothelial Growth Factor A genetics, Vascular Endothelial Growth Factor Receptor-2 genetics, Cell Movement physiology, Cerebellum physiology, Neurons physiology, Vascular Endothelial Growth Factor A metabolism, Vascular Endothelial Growth Factor Receptor-2 metabolism
Abstract

Vascular endothelial growth factor (VEGF) regulates angiogenesis, but also has important, yet poorly characterized roles in neuronal wiring. Using several genetic and in vitro approaches, we discovered a novel role for VEGF in the control of cerebellar granule cell (GC) migration from the external granule cell layer (EGL) toward the Purkinje cell layer (PCL). GCs express the VEGF receptor Flk1, and are chemoattracted by VEGF, whose levels are higher in the PCL than EGL. Lowering VEGF levels in mice in vivo or ectopic VEGF expression in the EGL ex vivo perturbs GC migration. Using GC-specific Flk1 knock-out mice, we provide for the first time in vivo evidence for a direct chemoattractive effect of VEGF on neurons via Flk1 signaling. Finally, using knock-in mice expressing single VEGF isoforms, we show that pericellular deposition of matrix-bound VEGF isoforms around PC dendrites is necessary for proper GC migration in vivo. These findings identify a previously unknown role for VEGF in neuronal migration.

Published
2010
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2. Matrix-Binding Vascular Endothelial Growth Factor (VEGF) Isoforms Guide Granule Cell Migration in the Cerebellum via VEGF Receptor Flk1.

Author

de Almodovar, Carmen Ruiz, Coulon, Cathy, Salin, Paul Antoine, Knevels, Ellen, Chounlamountri, Naura, Poesen, Koen, Hermans, Karlien, Lambrechts, Diether, van Geyte, Katie, Dhondt, Joke, Dresselaers, Tom, Renaud, Julie, Aragones, Julian, Zacchigna, Serena, Geudens, Ilse, Gall, David, Stroobants, Stijn, Mutin, Mireille, Dassonville, Karel, and Storkebaum, Erik

Subjects
VASCULAR endothelial growth factors, CELL migration, CEREBELLUM, NEOVASCULARIZATION, NEURAL circuitry, PURKINJE cells, MICRODIALYSIS, ECTOPIC hormones
Abstract

Vascular endothelial growth factor (VEGF) regulates angiogenesis, but also has important, yet poorly characterized roles in neuronal wiring. Using several genetic and in vitro approaches, we discovered a novel role for VEGF in the control of cerebellar granule cell (GC) migration from the external granule cell layer (EGL) toward the Purkinje cell layer (PCL). GCs express the VEGF receptor Flk1, and are chemoattracted by VEGF, whose levels are higher in the PCL than EGL. Lowering VEGF levels in mice in vivo or ectopic VEGF expression in the EGL ex vivo perturbsGCmigration. Using GC-specific Flk1 knock-out mice, we provide for the first time in vivo evidence for a direct chemoattractive effect of VEGF on neurons via Flk1 signaling. Finally, using knock-in mice expressing single VEGF isoforms, we show that pericellular deposition of matrix-bound VEGF isoforms around PC dendrites is necessary for proper GC migration in vivo. These findings identify a previously unknown role for VEGF in neuronal migration. [ABSTRACT FROM AUTHOR]

Published
2010
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3. Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1

Author

Mireille Mutin, Ulf Eriksson, Karlien Hermans, Alain Chédotal, Marie-Françoise Belin, Ellen Knevels, Peter Carmeliet, Carmen Ruiz de Almodovar, Claire Meissirel, Stefan Vinckier, Rudi D'Hooge, Naura Chounlamountri, Stijn Stroobants, Jody J. Haigh, Koen Poesen, Nicole Thomasset, Katie Van Geyte, Cathy Coulon, Paul Van Hecke, Bernard Gallez, Bénédicte F. Jordan, Paul A. Salin, Serena Zacchigna, Erik Storkebaum, Tom Dresselaers, Julian Aragones, Joke Dhondt, Julie Renaud, Jérôme Honnorat, Ilse Geudens, David Gall, Serge N. Schiffmann, Lieve Moons, Karel Dassonville, Diether Lambrechts, UCL - SSS/LDRI - Louvain Drug Research Institute, UCL - (SLuc) Service de médecine nucléaire, Institut National de la Santé et de la Recherche Médicale (INSERM), KUL, De Almodovar, Carmen Ruiz, Coulon, Cathy, Salin, Paul Antoine, Knevels, Ellen, Chounlamountri, Naura, Poesen, Koen, Hermans, Karlien, Lambrechts, Diether, Van Geyte, Katie, Dhondt, Joke, Dresselaers, Tom, Renaud, Julie, Aragones, Julian, Zacchigna, Serena, Geudens, Ilse, Gall, David, Stroobants, Stijn, Mutin, Mireille, Dassonville, Karel, Storkebaum, Erik, Jordan, Bénédicte F., Eriksson, Ulf, Moons, Lieve, D'Hooge, Rudi, Haigh, Jody J., Belin, Marie Françoise, Schiffmann, Serge, Van Hecke, Paul, Gallez, Bernard, Vinckier, Stefan, Chédotal, Alain, Honnorat, Jérôme, Thomasset, Nicole, Carmeliet, Peter, and Meissirel, Claire

Subjects
Neurons - cytology, physiology, Vascular Endothelial Growth Factor A, Cerebellum, Angiogenesis, [SDV]Life Sciences [q-bio], Apoptosis, Matrix (biology), Cerebellum - cytology, physiology, Transgenic, chemistry.chemical_compound, Mice, 0302 clinical medicine, HEK293 Cell, Cell Movement, Protein Isoforms, Vascular Endothelial Growth Factor A - genetics, metabolism, Apoptosis - physiology, ComputingMilieux_MISCELLANEOUS, Cells, Cultured, Neurons, 0303 health sciences, Microscopy, Cultured, Microscopy, Confocal, Blotting, Reverse Transcriptase Polymerase Chain Reaction, General Neuroscience, Articles, Sciences bio-médicales et agricoles, Growth Cone, Immunohistochemistry, Vascular Endothelial Growth Factor Receptor-2 - genetics, metabolism, Cell biology, Vascular endothelial growth factor, medicine.anatomical_structure, Confocal, Western, Human, Cells, Protein Isoforms - metabolism, Blotting, Western, Growth Cones, Enzyme-Linked Immunosorbent Assay, Mice, Transgenic, Biology, 03 medical and health sciences, In vivo, medicine, Animals, Humans, HEK293 Cells, Vascular Endothelial Growth Factor Receptor-2, Neuroscience (all), 030304 developmental biology, Animal, Growth Cones - metabolism, Apoptosi, Protein Isoform, Neuron, Granule cell, Molecular biology, In vitro, chemistry, Vascular Endothelial Growth Factor Receptor-2 -- genetics, Cell Movement - physiology, metabolism, 030217 neurology & neurosurgery, Ex vivo
Abstract

Vascular endothelial growth factor (VEGF) regulates angiogenesis, but also has important, yet poorly characterized roles in neuronal wiring. Using several genetic and in vitro approaches, we discovered a novel role for VEGF in the control of cerebellar granule cell (GC) migration from the external granule cell layer (EGL) toward the Purkinje cell layer (PCL). GCs express the VEGF receptor Flk1, and are chemoattracted by VEGF, whose levels are higher in the PCL than EGL. Lowering VEGF levels in mice in vivo or ectopic VEGF expression in the EGL ex vivo perturbs GC migration. Using GC-specific Flk1 knock-out mice, we provide for the first time in vivo evidence for a direct chemoattractive effect of VEGF on neurons via Flk1 signaling. Finally, using knock-in mice expressing single VEGF isoforms, we show that pericellular deposition of matrix-bound VEGF isoforms around PC dendrites is necessary for proper GC migration in vivo. These findings identify a previously unknown role for VEGF in neuronal migration., Journal Article, Research Support, Non-U.S. Gov't, SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2010
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