1. The centriolar satellite protein Cep131 is important for genome stability.
- Author
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Staples CJ, Myers KN, Beveridge RD, Patil AA, Lee AJ, Swanton C, Howell M, Boulton SJ, and Collis SJ
- Subjects
- Antigens, Neoplasm metabolism, Autoantigens metabolism, Cell Line, Chromosomal Instability, Cytoskeletal Proteins, Dynactin Complex, Dyneins metabolism, Humans, Microtubule-Associated Proteins metabolism, Mitosis genetics, Neoplasm Proteins metabolism, Cell Cycle Proteins genetics, Cell Cycle Proteins metabolism, Centrioles genetics, Centrioles ultrastructure, Centrosome metabolism, Centrosome ultrastructure, Genomic Instability, Microtubule Proteins genetics, Microtubule Proteins metabolism
- Abstract
The centrosome acts as a centre for microtubule organisation and plays crucial roles in cell polarity, migration, growth and division. Cep131 has recently been described as a basal body component essential for cilium formation, but its function in non-ciliogenic cells is unknown. We identified human Cep131 (also known as AZI1) in a screen for regulators of genome stability. We show that centrosomal localisation of Cep131 is cell-cycle-regulated and requires both an intact microtubule network and a functional dynein-dynactin transport system. Cep131 is recruited to centriolar satellites by PCM1, and localised to the centriolar core region by both pericentrin and Cep290. Depletion of Cep131 results in a reduction in proliferation rate, centriole amplification, an increased frequency of multipolar mitosis, chromosomal instability and an increase in post-mitotic DNA damage. These data therefore highlight the importance of human Cep131 for maintaining genomic integrity.
- Published
- 2012
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