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Albert Einstein Medicina Diagnostica Alka Tecnologia Amaral Costa Laboratorio Conselho Federal de Biomedicina DASA Euroimmun Brasil Grupo Fleury Hemagen Medivax Olimpus Padrao Laboratorio Clinic Pontificia Universidade Catolica de Goias - PUC-Goias Sociedade Brasileira de Patologia Clinica e Medicina Laboratorial Sociedade Brasileira de Reumatologia Thermo Scientific Wama Diagnostica Werfen Group - Werfen Medical Hermes Pardini Conselho Regional de Biomedicina - 3a Regiao Objetivo: O IV Consenso Brasileiro para Pesquisa de Autoanticorpos em Células HEp-2 (FAN) realizado em Vitória (ES), no dia 18 de setembro de 2012, objetivou discutir estratégias e recomendações relacionadas ao procedimento técnico, à padronização e à interpretação dos resultados da pesquisa de autoanticorpos em células HEp-2. Métodos: Participaram do evento 23 pesquisadores e especialistas de Universidades e laboratórios brasileiros. Foram abordados diferentes tópicos, discutidos amplamente a fim de se estabelecer recomendações específicas. Resultados e conclusão: O IV Consenso integrou à árvore de decisão o padrão citoplasmático em Anéis e Bastões, o padrão nuclear pontilhado Quasi-homogêneo (QH) e o padrão misto CENP-F. Discutiu-se ainda a necessidade de atenção para a classificação do padrão misto relacionado à presença de anticorpos anti-DNA topoisomerase I (Scl-70), compreendendo os componentes nuclear pontilhado fino, nucleolar homogêneo, NOR na placa metafásica e citoplasmático pontilhado fino. Foram sugeridas diretrizes para o controle de qualidade do teste, diluição de triagem e diluição de esgotamento, e foi emitido alerta quanto à necessidade de atenção em relação à heterogeneidade de substratos disponíveis no mercado e a utilização de metodologias automatizadas para detecção de autoanticorpos. Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved. PUC Goias, Goiania, Go, Brazil Fleury Med & Saude, São Paulo, Brazil Universidade Federal de São Paulo UNIFESP, Discipline Rheumatol, São Paulo, Brazil Univ Fed Uberlandia, Hosp Clin, Serv Rheumatol, BR-38400 Uberlandia, MG, Brazil Clin Doencas Reumat Porto Alegre, Ctr Diagnost Med & Rheuma, Porto Alegre, RS, Brazil Amaral Costa Med Diagnost, Belem, PA, Brazil Univ São Paulo, Fac Med, Hosp Clin, Lab Invest Med, São Paulo, Brazil Univ São Paulo, Fac Med, Hosp Clin, Lab Cent, São Paulo, Brazil Hosp Israelita Albert Einstein, Dept Clin Pathol, São Paulo, Brazil Inst Hermes Pardini, Belo Horizonte, MG, Brazil Univ São Paulo, Fac Med, São Paulo, Brazil Univ Fed Minas Gerais, Fac Med, Belo Horizonte, MG, Brazil Univ Catolica Brasilia, Brasilia, DF, Brazil Univ Fed Goias, Fac Med, Goiania, Go, Brazil Univ Sul Santa Catarina UNISUL, Florianopolis, SC, Brazil Univ Vale do Itajai UNIVALE, Florianopolis, SC, Brazil Univ Fed Espirito Santo, Vitoria, ES, Brazil EBMSP, Salvador, BA, Brazil Grp DASA, São Paulo, Brazil Escola Super Ciencias Saude Dist Fed, Brasilia, DF, Brazil Lab Sabin, Brasilia, DF, Brazil Universidade Federal de São Paulo UNIFESP, Discipline Rheumatol, São Paulo, Brazil Web of Science

ObjectiveThe Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitória, Espírito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.MethodsTwenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusionThe 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. The group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies.