1. Isolation and characterization of extracellular enzymes from endophytic bacteria isolated from Typhonium flagelliforme and Typhonium blumei.
- Author
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Lekatompessy, Sylvia J. R., Puteri, Sella Amelia, Simarmata, Rumella, Widowati, Tiwit, Erdayani, Eva, Windiastri, Vincentia Esti, Nurhasanah, Ade Nena, Widyajayantie, Dwi, Nurjanah, Liseu, Nuriyanah, Maulana, Budi Satrio, Erlinawati, Ina, Wahyuni, Sri, Harmoko, Rikno, Nuryana, Isa, Izzati, Fauzia Nurul, Sarwono, Ki Ageng, Khaerunnisa, Isyana, Rahmawati, Siti Irma, and Bayu, Asep
- Subjects
EXTRACELLULAR enzymes ,ENDOPHYTIC bacteria ,AMYLASES ,CELLULASE ,GELATIN ,TARO ,STARCH - Abstract
Fifty-nine endophytic bacterial isolates from rat taro have been obtained; thirty-four bacterial isolates from rat taro type 3 (TF3) and twenty-five bacterial isolates were from type 4 (TF4). The extracellular enzyme activity was observed qualitatively from rat taro endophytic bacterial isolates. The amylase activity test used starch as substrate, whereas the protease test used gelatin. A cellulose activity test was carried out using Carboxy Methyl Cellulase (CMC) media. The results showed that twenty-seven endophytic bacterial isolates of rat taro TF3 and TF4 showed extracellular enzyme activity. Endophytic bacterial isolate TF3Bt4 had the highest protease activity, indicated by its lytic index of about 11.304 mm in the gelatin-containing medium. The highest amylase activity was produced by isolate TF3U3, where the lytic index was 23.875 mm in the starch containing medium. Bacterial isolate TF3A4 showed a lytic index of about 6.9653 mm in the CMC medium, indicating the highest cellulase activity. The endophytic bacteria with protease activity are potentially utilized in the food industry, whereas endophytic bacteria with cellulase activity can be used in the textile industry. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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