1. The Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile
- Author
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Sebastian, Thieme, Alexander, Holzbaur, Ralf, Wiedemuth, Aline, Binner, Katrin, Navratiel, Konstantinos, Anastassiadis, Sebastian, Brenner, and Cornelia, Richter
- Subjects
Plasmacytoid dendritic cells, blood cell population, TU Dresden, Publishing Fund ,Physiology ,Immune Cells ,Immunology ,Antigen-Presenting Cells ,lcsh:Medicine ,Cytotoxic T cells ,macromolecular substances ,Research and Analysis Methods ,Mice ,White Blood Cells ,Spectrum Analysis Techniques ,Animal Cells ,Immune Physiology ,Medicine and Health Sciences ,Animals ,ddc:610 ,Molecular Biology Techniques ,lcsh:Science ,Molecular Biology ,Immune Response ,Secretion ,Cell Line, Transformed ,Innate Immune System ,Blood Cells ,T Cells ,lcsh:R ,Cell Polarity ,Biology and Life Sciences ,hemic and immune systems ,Dendritic Cells ,Cell Biology ,Molecular Development ,Flow Cytometry ,Spectrophotometry ,Doxycycline ,Immune System ,Cytokines ,lcsh:Q ,Cytophotometry ,Cellular Types ,Physiological Processes ,Research Article ,Developmental Biology ,Cloning ,Plasmazytoide dendritische Zellen, Blutzellpopulation, TU Dresden, Publikationsfonds - Abstract
Plasmacytoid dendritic cells (pDC) constitute a very rare blood cell population and play a significant role in immune response and immune-mediated disorders. Investigations on primary pDCs are hindered not only due to their rarity but also because they represent a heterogeneous cell population which is difficult to culture ex vivo. We generated a conditionally immortalized pDC line (Dox-pDC) from mice with Doxycycline-inducible SV40 Large T Antigen with a comparable immune profile to primary pDCs. The Dox-pDC secrete pro- and anti-inflammatory cytokines upon Toll-like receptor 9 stimulation and upregulate their MHCI, MHCII and costimulatory molecules. Further, the Dox-pDC activate and polarize naïve T cells in vivo and in vitro in response to the model antigen Ovalbumin. Due to their long-term culture stability and their robust proliferation Dox-pDC represent a reliable alternative to primary mouse pDC.
- Published
- 2018