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Your search keyword '"PHA, phytohaemagglutinin"' showing total 3 results
Start Over Descriptor "PHA, phytohaemagglutinin" Topic cellular immunity
3 results on '"PHA, phytohaemagglutinin"'

1. In vitro proliferation of blood, spleen and thymus leukocytes from the turtle Mauremys caspica

Author

Muñoz, Francisco J. and De la Fuente, Mónica

Subjects
*CELLULAR immunity, *IMMUNE response, *CELL proliferation, *MITOGENS
Abstract

Cell-mediated immune responses is commonly evaluated by cell proliferation assays. Mitogens are known to induce a vigorous proliferative response in lymphoid cells from mammals but relatively fewer studies have investigated mitogen-mediated lymphoproliferation in non-mammalian animals. In the present work, we incubated spleen, thymus and blood leukocytes with phytohaemagglutinin (PHA), concanavalin A (Con A), lipopolysaccharide (LPS) and pokeweed mitogen (PWM), by different times of incubation (96 and 120 h) and at different concentrations. Our results show that the optimal mitogen concentrations inducing proliferation on leukocytes from Mauremys caspica were 20 μg/ml PHA, 1 μg/ml Con A, 12.5 μg/ml LPS and 1/150 dilution PWM. The optimal time of incubation was dependent on the type of leukocytes (peripheral blood leukocytes, splenic leukocytes or thymic cells) and the mitogen utilized. [Copyright &y& Elsevier]

Published
2003
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2. Weak binder for MHC molecule is a potent Mycobacterium tuberculosis-specific CTL epitope in the context of HLA-A24 allele

Author

Meiyi Sun, Limin Shi, Junxian Zhang, Yan Wang, Jie Ding, Min He, Honglian Cui, Bin Gao, Wei Wang, and Wenjiong Xu

Subjects
Male, Cellular immunity, TB, tuberculosis, AFB, acid-fast bacilli, Epitopes, T-Lymphocyte, Genes, MHC Class I, HLA-A24 Antigen, Epitope, CFP10, 10-KDa culture filtrate protein, ESAT-6, rhIL-7, recombinant human interleukine-7, Cells, Cultured, BCG, bacillus Calmette-Guérin, ELISPOT, HLA-A24, LTBI, latent TB infection, Middle Aged, Infectious Diseases, HPLC, high performance liquid chromatography, Cytokines, Female, Adult, CTLs, cytotoxic T lymphocytes, Adolescent, Molecular Sequence Data, Mtb, Mycobacterium tuberculosis, chemical and pharmacologic phenomena, Human leukocyte antigen, Biology, Major histocompatibility complex, Microbiology, Article, SFCs, spot-forming cells, Young Adult, Species Specificity, Antigen, MHC, major histocompatibility complex, Humans, Tuberculosis, Amino Acid Sequence, rhIL-2, recombinant human interleukine-2, PBMCs, peripheral blood mononuclear cells, PHA, Phytohaemagglutinin, IFN-γ, interferon gamma, HLA, human leukocyte antigen, ESAT-6, 6 kDa early secretory antigenic target, Mycobacterium tuberculosis, PE, phycoerythrin, Virology, Immunology, Leukocytes, Mononuclear, biology.protein, β2m, β2 microglobulin
Abstract

s Tuberculosis causes serious health problem for the world population. Antigenic peptides selected by pathogen-specific cytotoxic T lymphocytes (CTLs) are presented by major histocompatibility complex (MHC; or human leukocyte antigen [HLA] in humans) molecules, and HLA-A restricted responses may be of interest for vaccine development and the understanding of cellular immunity. A series of peptides derived from the 10-KDa culture filtrate protein (CFP10) and the 6 kDa early secretory antigenic target (ESAT-6) in the Mycobacterium tuberculosis (Mtb) have been screened and a CTL epitope restricted by the human leukocyte antigen HLA-A24, a common HLA allele in Asian people, has been identified. In this study, we studied a panel of CFP10 and ESAT-6-derived peptides to identify those with binding motifs for HLA-A24 molecules. The antigenicity of candidate peptides was assessed with in vitro refolding tests and an enzyme-linked immunospot (ELISPOT) assay, and by tetramer staining to determine the capacity to stimulate CTLs from peripheral blood mononuclear cells (PBMCs) of HLA-A24-positive TB Patients. We report that one novel candidate peptide at positions 5–14 of ESAT-6 of Mtb could induce peptide-specific CTLs from PBMCs of HLA-A24-positive patients, but not from HLA-A24-negative patients and HLA-A24-positive healthy controls. Identified epitope is a weak binder for HLA-A24 molecule in a mini MHC refolding assay. Since the peptide is presented by a common HLA class I molecule, it may be useful for immunotherapy against Mtb infection and vaccine development in the large population of Mtb-infected patients., Highlights ► We study a panel of Mtb-derived peptides to identify binders for HLA-A24. ► Peptide P5 significantly elicits IFN-producing CTLs from HLA-A24+ TB donors. ► Peptide P5 is a weak binder for HLA-A24 molecule in a mini refolding assay. ► The identified epitope may be useful for immunotherapy and vaccine development.

Published
2012
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3. MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines

Author

Tamara Berthoud, Stephen Todryk, Susanna Dunachie, Helen A. Fletcher, and Adrian V. S. Hill

Subjects
Male, Cellular immunity, ESAT6, six-kDa early secreted antigenic target, T-Lymphocytes, STAT, signal transducers and activators of transcription, MVA, modified vaccinia virus Ankara, APC, antigen presenting cell, Chemokine CXCL9, ELISA, enzyme linked immunoSorbant assay, FP9, fowlpox strain FP9, BME, β-mercaptoethanol, Immunology and Allergy, M, MVA, Interferon gamma, Flow cytometry, Malaria, Falciparum, PPD, purified protein derivative, IFN-γ, RLT, RNA lysis buffer, Vaccines, Synthetic, HPRT, hypoxanthine phosphoribosyl transferase, medicine.diagnostic_test, Malaria vaccine, Reverse Transcriptase Polymerase Chain Reaction, ELISPOT, MIG, Middle Aged, MIG, monokine induced by gamma, TRAP, thrombospondin related adhesive protein, Monokine, P, PEV3A, ELISPOT, enzyme linked immuno SPOT, PBMC, peripheral blood mononuclear cells, CXCL9, F, FP9, Female, medicine.drug, Research Paper, Adult, CSP, circumsporozoite protein, Adolescent, SEB, staphylococcal enterotoxin B, Immunology, Plasmodium falciparum, Biology, JAK, janus kinase, Interferon-gamma, Young Adult, RT-PCR, reverse transcription polymerase chain reaction, stomatognathic system, RPMI, Roswell Park Memorial Institute media, Malaria Vaccines, medicine, Animals, Humans, RNA, Messenger, AMA1, apical membrane antigen 1, 7AAD, 7-amino-actinomycin D, Aged, CFP10, culture filtrate protein 10, DOC, day of challenge, Brefeldin A, IFN-γ, interferon gamma, CXCL9, CXC chemokine ligand 9, CMV, cytomegalovirus, EBV, Epstein Barr Virus, Virology, Malaria, stomatognathic diseases, PHA, phytohaemagglutinin, Vaccine, Ex vivo, ICS, intracellular staining, ME, multi-epitope string
Abstract

For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination.

Published
2009

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