1. In vivo single-cell labeling by confined primed conversion.
- Author
-
Dempsey, William P, Georgieva, Lada, Helbling, Patrick M, Sonay, Ali Y, Truong, Thai V, Haffner, Michel, and Pantazis, Periklis
- Subjects
- *
FLUORESCENT proteins , *PULSED lasers , *ZEBRA danio , *NEURONS , *CELLS , *SCIENTIFIC method - Abstract
Spatially confined green-to-red photoconversion of fluorescent proteins with high-power, pulsed laser illumination is negligible, thus precluding optical selection of single cells in vivo. We report primed conversion, in which low-power, dual-wavelength, continuous-wave illumination results in pronounced photoconversion. With a straightforward addition to a conventional confocal microscope, we show confined primed conversion in living zebrafish and reveal the complex anatomy of individual neurons packed between neighboring cells. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF