1. [Preparation of a novel targeted MR contrast agent Gd-DTPA-Granzyme B monoclonal antibody]
- Author
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Yi, Xie, Guang-yi, Liu, Fei, Han, Hong, Jiang, You-ying, Mao, Hui-ping, Wang, Jie, Zhao, Juan, Jin, and Jiang-hua, Chen
- Subjects
Gadolinium DTPA ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Antibodies, Monoclonal ,Contrast Media ,Magnetic Resonance Imaging ,Cells, Cultured ,Granzymes - Abstract
To prepare a novel MRI targeted contrast agent Gd-DTPA-Granzyme B monoclonal antibody (mAb) and to test its reaction conditions.The Granzyme B mAb was coupled with DTPA,and then conjugated with Gd. The Gd-DTPA antibody was characterized using MALDI-TOF-MS. Cytotoxicity test was performed with MTT assay, and immune activation was examined with immunohistochemistry.MALDI-TOF-MS demonstrated that the molecular weight shifted from granzyme B mAb (133986) to Gd-DTPA-GB mAb (139736), which indicated the conjugation of the antibody with Gd-DTPA. The molar ratio of Gd per IgG molecule was about 20. MTT assay showed that Gd, DTPA, Gd-DTPA and Gd-DTPA-GB mAb groups did not make an impact on cell viability, and there were no significant differences among 4 groups (P0.05). Immunohistochemistry results showed that compared with the positive control group the targeted contrast agent had a high immune activity.The novel contrast agent Gd-DTPA-Granzyme B mAb prepared in this study keeps a good immune activity and has no significant cytotoxicity.
- Published
- 2010