Search

Objectives: Engineered Heart Tissue (EHT) is a promising tool to repair heart muscle defects and can additionally be used for drug testing. Due to the absence of an in vitro vascularization, EHT geometry crucially impacts nutrient and oxygen supply by diffusion capacity. We analyzed cardiomyocyte survival in different EHT geometries., Methods: Different geometries with varying surface-area-to-volume-ratios were calculated (structure A (Ring) AS/V = 58.47 mm 2 /440 μL 3 , structure B (Infinity) 25.86 mm 2 /440 μL 3 ). EHTs were generated from hiPSC-derived cardiomyocytes (4 × 10 6 ) and a fibrin/thrombin hydrogel. Cell viability was evaluated by RT-PCR, cytometric studies, and Bioluminescence imaging., Results: Using 3D-printed casting molds, spontaneously beating EHTs can be generated in various geometric forms. At day 7, the RT-PCR analyses showed a significantly higher Troponin-T value in ring EHTs, compared to infinity EHTs. In cytometric studies, we evaluated 15% more Troponin-T positive cells in ring (73% ± 12%), compared to infinity EHTs (58% ± 11%, p = 0.04). BLI visualized significantly higher cell survival in ring EHTs (ROI = A: 1.14 × 10 6 p/s and B: 8.47 × 10 5 p/s, p < 0.001) compared to infinity EHTs during longitudinal cultivation process., Conclusion: Use of 3D-printing allows the creation of EHTs in all desired geometric shapes. The geometry with an optimized surface-area-to-volume-ratio (ring EHT) demonstrated a significantly higher cell survival measured by RT-PCR, Bioluminescence imaging, and cytometric studies using FACS analysis., (© 2024 The Author(s). Artificial Organs published by International Center for Artificial Organ and Transplantation (ICAOT) and Wiley Periodicals LLC.)

This article discusses the use of 3D-printed geometric shapes in creating Engineered Heart Tissue (EHT) for repairing heart muscle defects and drug testing. The researchers analyzed the surface-area-to-volume ratio (AS/V) of different shapes to determine the ideal EHT shape for cell survival. They found that structure A had higher cell survival compared to structure B, as evidenced by histological experiments, gene expression analysis, cytometric studies, and bioluminescence imaging. The study concludes that optimizing the geometry of EHTs can significantly improve cell survival. [Extracted from the article]