1. Curcumin effects on cell proliferation, angiogenesis and metastasis in colorectal cancer.
- Author
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Calibasi-Kocal G, Pakdemirli A, Bayrak S, Ozupek NM, Sever T, Basbinar Y, Ellidokuz H, and Yigitbasi T
- Subjects
- Cell Line, Tumor, Cell Survival drug effects, Colorectal Neoplasms pathology, Curcuma, Curcumin chemistry, HCT116 Cells, Humans, Neoplasm Metastasis, Neovascularization, Pathologic pathology, Plant Extracts pharmacology, Cell Proliferation drug effects, Colorectal Neoplasms drug therapy, Curcumin pharmacology, Neovascularization, Pathologic drug therapy
- Abstract
Purpose: Curcumin is a natural phytopolyphenol compound isolated from the root of turmeric (Curcuma longa) and possesses a wide range of biological properties. The purpose of this study was to evaluate the antiproliferative, wound healing, anti-invasive and anti-migrative effects of curcumin on HCT-116 and LoVo colorectal cancer cell lines., Methods: The antiproliferative activity of 2.5-75 µM curcumin was tested on HCT-116 and LoVo colorectal cell lines and the viability of the cells was tested with WST-1 reagent by using ELISA plate reader at 450 nm. xCELLigence RTCA DP system was used for the detection of anti-invasive and anti-migrative effects of curcumin., Results: The IC50 of curcumin was 10±0.03 for HCT-116 and 20±0.05 µM for LoVo cell lines. The IC50 of curcumin (10µM for HCT-116 and 20 µM for LoVo) showed anti-metastatic activity on these cell lines., Conclusion: This study showed that curcumin could be evaluated as a promising anti-cancer agent for human colorectal cancer.
- Published
- 2019