1. Bone marrow-derived mesenchymal stromal cells promote colorectal cancer cell death under low-dose irradiation.
- Author
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Feng H, Zhao JK, Schiergens TS, Wang PX, Ou BC, Al-Sayegh R, Li ML, Lu AG, Yin S, and Thasler WE
- Subjects
- Apoptosis radiation effects, Bone Marrow Cells, Caspase 3 metabolism, Cell Differentiation, Coculture Techniques, Epithelial-Mesenchymal Transition, HT29 Cells, Humans, Interferon-gamma metabolism, MAP Kinase Signaling System radiation effects, Mesenchymal Stem Cells physiology, Organoids, Phosphatidylinositol 3-Kinase metabolism, Phosphorylation radiation effects, Proto-Oncogene Proteins c-akt metabolism, Tumor Necrosis Factor-alpha metabolism, Ultraviolet Rays, X-Rays, Cell Proliferation radiation effects, Cell Survival radiation effects, Colorectal Neoplasms radiotherapy, Mesenchymal Stem Cells metabolism, Mesenchymal Stem Cells radiation effects
- Abstract
Background: Radiotherapy remains one of the cornerstones to improve the outcome of colorectal cancer (CRC) patients. Radiotherapy of the CRC not only help to destroy cancer cells but also remodel the tumour microenvironment by enhancing tumour-specific tropism of bone marrow-derived mesenchymal stromal cell (BM-MSC) from the peripheral circulation. However, the role of local MSCs and recruited BM-MSC under radiation were not well defined. Indeed, the functions of BM-MSC without irradiation intervention remained controversial in tumour progression: BM-MSC was previously shown to modulate the immune function of major immune cells, resulting in an impaired immunological sensitivity and to induce an increased risk of tumour recurrence. In contrast, it could also secrete various cytokines and possess anticancer effect., Methods: Three co-cultivation modules, 3D culture modules, and cancer organoids were established. The induction of cytokines secretion in hBM-MSCs after irradiation was analysed by ELISA array and flow cytometry. AutoMac separator was used to separate hBM-MSC and CRC automatically. Cells from the co-cultured group and the control group were then irradiated by UV-C lamp and X-ray. Proliferation assay and viability assay were performed., Results: In this study, we show that BM-MSCs can induce the EMT progression of CRC cells in vitro. When irradiated with low doses of ultraviolet radiation and X-rays, BM-MSCs show an anti-tumour effect by secreting certain cytokine (TNF-α, IFN-γ) that lead to the inhibition of proliferation and induction of apoptosis of CRC cells. This was further verified in a 3D culture model of a CRC cell in vitro. Furthermore, irradiation on the co-culture system induced the cleavage of caspase3, and attenuated the phosphorylation of phosphatidylinositol 3-kinase (PI3K)/AKT and extracellular signal-regulated kinase in cancer cells. The signal pathways above might contribute to the cancer cell death., Conclusions: Taken together, we show that BM-MSC can potentially promote the effect of radiotherapy in CRC.
- Published
- 2018
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