1. Genomics and proteomics approaches to the study of cancer-stroma interactions
- Author
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Flávia C, Rodrigues-Lisoni, Paulo, Peitl, Alessandra, Vidotto, Giovana M, Polachini, José V, Maniglia, Juliana, Carmona-Raphe, Bianca R, Cunha, Tiago, Henrique, Caique F, Souza, Rodrigo A P, Teixeira, Erica E, Fukuyama, Pedro, Michaluart, Marcos B, de Carvalho, Sonia M, Oliani, Eloiza H, Tajara, P M, Cury, M B, de Carvalho, E, Dias-Neto, D L A, Figueiredo, E E, Fukuyama, J F, Góis-Filho, A M, Leopoldino, R C M, Mamede, P, Michaluart-Junior, R A, Moyses, F G, Nóbrega, M P, Nóbrega, F D, Nunes, E F B, Ojopi, L N, Serafini, P, Severino, A M A, Silva, W A, Silva, N J F, Silveira, S C O M, Souza, E H, Tajara, V, Wünsch-Filho, A, Amar, C M, Bandeira, M A, Braconi, L G, Brandão, R M, Brandão, A L, Canto, M, Cerione, R, Cicco, M J, Chagas, H, Chedid, A, Costa, B R, Cunha, O A, Curioni, C S, Fortes, S A, Franzi, A P Z, Frizzera, D, Gazito, P E M, Guimarães, C M, Kaneto, R V M, López, R, Macarenco, M R, Magalhães, C, Meneses, A M C, Mercante, D G, Pinheiro, G M, Polachini, A, Rapoport, C O, Rodini, F C, Rodrigues-Lisoni, R V, Rodrigues, L, Rossi, A R D, Santos, M, Santos, F, Settani, F A M, Silva, I T, Silva, T B, Souza, E, Stabenow, J T, Takamori, P J, Valentim, A, Vidotto, F C A, Xavier, F, Yamagushi, M L, Cominato, P M S, Correa, G S, Mendes, R, Paiva, O, Ramos, C, Silva, M J, Silva, M V C, Tarlá, Faculdade de Medicina de São José do Rio Preto (FAMERP), Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), Arnaldo Vieira de Carvalho Hosp, and Heliopolis Hosp
- Subjects
lcsh:Internal medicine ,Stromal cell ,lcsh:QH426-470 ,Proteome ,Down-Regulation ,Apoptosis ,Biology ,Proteomics ,Paracrine signalling ,Gene expression ,Research article ,Genetics ,medicine ,Humans ,Vimentin ,Genetics(clinical) ,Electrophoresis, Gel, Two-Dimensional ,Annexin A5 ,lcsh:RC31-1245 ,Genetics (clinical) ,Cell Proliferation ,Regulation of gene expression ,Tumor microenvironment ,Subtraction hybridization ,Cancer ,Nucleic Acid Hybridization ,Genomics ,Hep G2 Cells ,Fibroblasts ,medicine.disease ,Molecular biology ,Gene Expression Regulation, Neoplastic ,lcsh:Genetics ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Cancer research ,Keratins ,Mouth Neoplasms ,Stromal Cells - Abstract
Made available in DSpace on 2013-08-28T14:07:40Z (GMT). No. of bitstreams: 1 WOS000278383600001.pdf: 960234 bytes, checksum: 9cd26a9750ec13e67eaf9019e1f48e7b (MD5) Made available in DSpace on 2013-09-30T18:12:07Z (GMT). No. of bitstreams: 2 WOS000278383600001.pdf: 960234 bytes, checksum: 9cd26a9750ec13e67eaf9019e1f48e7b (MD5) WOS000278383600001.pdf.txt: 69473 bytes, checksum: e718db9d53c8e8829f735f82524e401f (MD5) Previous issue date: 2010-05-04 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:00:48Z No. of bitstreams: 2 WOS000278383600001.pdf: 960234 bytes, checksum: 9cd26a9750ec13e67eaf9019e1f48e7b (MD5) WOS000278383600001.pdf.txt: 69473 bytes, checksum: e718db9d53c8e8829f735f82524e401f (MD5) Made available in DSpace on 2014-05-20T14:00:48Z (GMT). No. of bitstreams: 2 WOS000278383600001.pdf: 960234 bytes, checksum: 9cd26a9750ec13e67eaf9019e1f48e7b (MD5) WOS000278383600001.pdf.txt: 69473 bytes, checksum: e718db9d53c8e8829f735f82524e401f (MD5) Previous issue date: 2010-05-04 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Rede Proteoma do Estado de São Paulo Ludwig Institute for Cancer Research Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Background: The development and progression of cancer depend on its genetic characteristics as well as on the interactions with its microenvironment. Understanding these interactions may contribute to diagnostic and prognostic evaluations and to the development of new cancer therapies. Aiming to investigate potential mechanisms by which the tumor microenvironment might contribute to a cancer phenotype, we evaluated soluble paracrine factors produced by stromal and neoplastic cells which may influence proliferation and gene and protein expression.Methods: The study was carried out on the epithelial cancer cell line (Hep-2) and fibroblasts isolated from a primary oral cancer. We combined a conditioned-medium technique with subtraction hybridization approach, quantitative PCR and proteomics, in order to evaluate gene and protein expression influenced by soluble paracrine factors produced by stromal and neoplastic cells.Results: We observed that conditioned medium from fibroblast cultures (FCM) inhibited proliferation and induced apoptosis in Hep-2 cells. In neoplastic cells, 41 genes and 5 proteins exhibited changes in expression levels in response to FCM and, in fibroblasts, 17 genes and 2 proteins showed down-regulation in response to conditioned medium from Hep-2 cells (HCM). Nine genes were selected and the expression results of 6 down-regulated genes (ARID4A, CALR, GNB2L1, RNF10, SQSTM1, USP9X) were validated by real time PCR.Conclusions: A significant and common denominator in the results was the potential induction of signaling changes associated with immune or inflammatory response in the absence of a specific protein. Sch Med FAMERP, Dept Mol Biol, Sao Jose do Rio Preto, Brazil São Paulo State Univ UNESP, IBILCE, Dept Biol, Sao Jose do Rio Preto, Brazil Sch Med FAMERP, Dept Otorhinolaryngol & Head & Neck Surg, Sao Jose do Rio Preto, Brazil Univ São Paulo, Inst Biociencias, Dept Genet & Evolutionary Biol, São Paulo, Brazil Arnaldo Vieira de Carvalho Hosp, Dept Head & Neck Surg, São Paulo, Brazil Univ São Paulo, Sch Med, Dept Surg, Div Head & Neck Surg, BR-09500900 São Paulo, Brazil Heliopolis Hosp, Dept Head & Neck Surg, São Paulo, Brazil UNESP, Fac Engn Ilha Solteria, Dept Biol & Zootechny, Ilha Solteira, Brazil São Paulo State Univ UNESP, IBILCE, Dept Biol, Sao Jose do Rio Preto, Brazil UNESP, Fac Engn Ilha Solteria, Dept Biol & Zootechny, Ilha Solteira, Brazil FAPESP: 04/12054-9 FAPESP: 06/60162-0 FAPESP: 04/14846-0 FINEP: 01.07.0290.00
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- 2010