1. Differential Sensitivity of Target Genes to Translational Repression by miR-17~92.
- Author
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Jin, Hyun Yong, Oda, Hiroyo, Chen, Pengda, Yang, Chao, Zhou, Xiaojuan, Kang, Seung Goo, Valentine, Elizabeth, Kefauver, Jennifer, Liao, Lujian, Zhang, Yaoyang, Gonzalez-Martin, Alicia, Shepherd, Jovan, Morgan, Gareth J., Mondala, Tony S., Head, Steven R., Kim, Pyeung-Hyeun, Xiao, Nengming, Fu, Guo, Liu, Wen-Hsien, and Han, Jiahuai
- Subjects
MICRORNA genetics ,GENE expression ,MOLECULAR genetics ,GENETIC transformation ,GENETIC transcription ,PHYSIOLOGY - Abstract
MicroRNAs (miRNAs) are thought to exert their functions by modulating the expression of hundreds of target genes and each to a small degree, but it remains unclear how small changes in hundreds of target genes are translated into the specific function of a miRNA. Here, we conducted an integrated analysis of transcriptome and translatome of primary B cells from mutant mice expressing miR-17~92 at three different levels to address this issue. We found that target genes exhibit differential sensitivity to miRNA suppression and that only a small fraction of target genes are actually suppressed by a given concentration of miRNA under physiological conditions. Transgenic expression and deletion of the same miRNA gene regulate largely distinct sets of target genes. miR-17~92 controls target gene expression mainly through translational repression and 5’UTR plays an important role in regulating target gene sensitivity to miRNA suppression. These findings provide molecular insights into a model in which miRNAs exert their specific functions through a small number of key target genes. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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