1. Involvement of syk and VEGF-C in invasion of lung adenocarcinoma A549 cells.
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Qifeng Sun, Chuanliang Peng, Bo Cong, Yingtao Hao, Jiazhong Guo, Yunpeng Zhao, Xiaogang Zhao, Sun, Qifeng, Peng, Chuanliang, Cong, Bo, Hao, Yingtao, Guo, Jiazhong, Zhao, Yunpeng, and Zhao, Xiaogang
- Subjects
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LUNG cancer diagnosis , *VASCULAR endothelial growth factor receptors , *LUNG cancer patients , *PROTEIN-tyrosine kinases , *MESSENGER RNA , *CELL receptors , *ADENOCARCINOMA , *CANCER invasiveness , *LUNG tumors , *METASTASIS , *FETAL development , *ENDOTHELIAL growth factors , *CELL physiology - Abstract
Background and Aims: Lung cancer has become one of the most dangerous malignant tumors in the world nowadays, whose pathogenesis is complex involving multi-genes and multi-elements. This study aims to investigate the values of spleen tyrosine kinase (Syk) and vascular endothelial growth factor-C (VEGF-C) in lymphangiogenesis and metastasis of lung adenocarcinoma A549 cells.Materials and Methods: The pcDNA3.1-VEGF-C and pLNCX-syk were constructed and transfected into A549 cells. After cells with stable expression were sorted, the level of VEGF-C was tested by RT-PCR and immunohistochemistry and the mRNA of syk was tested by RT-PCR. The cell invasion assay was investigated by transwell chamber in vitro. Restriction enzyme digestion and gel electrophoresis demonstrated successful construction of the pcDNA3.1-VEGF-C.Results: RT-PCR and immunohistochemistry revealed higher expression of VEGF-C in VEGFC-construct-transfected A549 cells than that in controls (P < 0.05). Successful construction of the pLNCX-syk was demonstrated by restriction enzyme electrophoresis and sequencing. RT-PCR revealed Syk expression higher in syk-construct-transfected cells than in controls (P < 0.05).Conclusions: The results indicate a potential link between the upregulation of Syk and VEGF-C expression and lung adenocarcinoma. [ABSTRACT FROM AUTHOR]- Published
- 2016
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