1. Angiogenic Potential of Multipotent Stromal Cells from the Umbilical Cord: an In Vitro Study
- Author
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E. Sh. Raimova, G. B. Bol’shakova, Gennady T. Sukhikh, E. Yu. Kananykhina, T. Kh. Fatkhudinov, A. V. Makarov, Andrey Elchaninov, and I. V. Arutyunyan
- Subjects
Vascular Endothelial Growth Factor A ,0301 basic medicine ,Stromal cell ,Cellular differentiation ,medicine.medical_treatment ,Neovascularization, Physiologic ,Umbilical cord ,General Biochemistry, Genetics and Molecular Biology ,Umbilical Cord ,03 medical and health sciences ,Paracrine signalling ,Cell Movement ,medicine ,Humans ,Cells, Cultured ,Cell Proliferation ,Chemistry ,Growth factor ,Mesenchymal stem cell ,Endothelial Cells ,Cell Differentiation ,Mesenchymal Stem Cells ,General Medicine ,Cord lining ,Cell biology ,Vascular endothelial growth factor A ,030104 developmental biology ,medicine.anatomical_structure ,Culture Media, Conditioned - Abstract
The mechanisms of proangiogenic activity of multipotent stromal cells from human umbilical cord were analyzed in vitro. The absence of secreted forms of proangiogenic growth factor VEGF-A in the culture medium conditioned by umbilical cord-derived multipotent stromal cells was shown by ELISA. However, the possibility of paracrine stimulation of cell proliferation, mobility, and directed migration of endothelial EA.hy926 cells was demonstrated by using MTT test, Transwell system, and monolayer wound modeling. The capacity of multipotent stromal cells to acquire the phenotype of endothelium-like cells was analyzed using differentiation media of three types. It was found that VEGF-A is an essential but not sufficient inductor of differentiation of umbilical cord-derived multipotent stromal cells into CD31+ cells.
- Published
- 2016
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