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Your search keyword '"Zahm, Jean-Marie"' showing total 6 results
Start Over Author "Zahm, Jean-Marie" Topic cell migration
6 results on '"Zahm, Jean-Marie"'

2. Epigallocatechin-3-gallate (EGCG) inhibits the migratory behavior of tumor bronchial epithelial cells.

Author

Hazgui, Salma, Bonnomet, Arnaud, Nawrocki-Raby, Béatrice, Milliot, Magali, Terryn, Christine, Cutrona, Jérôme, Polette, Myriam, Birembaut, Philippe, and Zahm, Jean-Marie

Subjects
EPIGALLOCATECHIN gallate, TUMORS, EPITHELIAL cells, BRONCHI, CELL migration
Abstract

Background: Many studies associated the main polyphenolic constituent of green tea, (-)-Epigallocatechin-3-gallate (EGCG), with inhibition of cancers, invasion and metastasis. To date, most of the studies have focused on the effect of EGCG on cell proliferation or death. Since cell migration is an important mechanism involved in tumor invasion, the aim of the present work was to target another approach of the therapeutic effect of EGCG, by investigating its effect on the cell migratory behavior. Methods: The effect of EGCG (at concentrations lower than 10 µg/ml) on the migration speed of invasive cells was assessed by using 2D and 3D models of cell culture. We also studied the effects of EGCG on proteinases expression by RT-PCR analysis. By immunocytochemistry, we analyzed alterations of vimentin organization in presence of different concentrations of EGCG. Results: We observed that EGCG had an inhibitory effect of cell migration in 2D and 3D cell culture models. EGCG also inhibited MMP-2 mRNA and protein expression and altered the intermediate filaments of vimentin. Conclusion: Taken together, our results demonstrate that EGCG is able to inhibit the migration of bronchial tumor cells and could therefore be an attractive candidate to treat tumor invasion and cell migration. [ABSTRACT FROM AUTHOR]

Published
2008
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3. Beta[sub 1]-Integrins are involved in migration of human fetal tracheal epithelial cells and...

Author

Coraux, Christelle and Zahm, Jean-Marie

Subjects
*INTEGRINS, *EPITHELIAL cells, *MORPHOGENESIS, *CELL migration
Abstract

Examines the role of beta[sub 1]-integrins in the migration of human fetal tracheal epithelial cells and tubular morphogenesis. Immunolocalization of beta[sub 1-]integrins; Detection in the cytoplasm of epithelial cells; Alteration of the morphology of migrating cells.

Published
2000

4. Video-microscopic imaging of cell spatio-temporal dispersion and migration

Author

Terryn, Christine, Bonnomet, Arnaud, Cutrona, Jérôme, Coraux, Christelle, Tournier, Jean-Marie, Nawrocki-Raby, Béatrice, Polette, Myriam, Birembaut, Philippe, and Zahm, Jean-Marie

Subjects
*CELLS, *DIAGNOSTIC imaging, *MICROSCOPY, *CELL migration
Abstract

Abstract: Live-cell imaging has become a powerful analytical tool in most cell biology laboratories. The scope of this paper is to give an overview of the environmental considerations for maintaining living cells on the microscope stage and the technical advances permitting multi-parameter imaging. The paper will then focus on two-dimensional and three-dimensional analysis of cell dispersion and migration and finally give a brief insight on computational modeling of the cell behavior. [Copyright &y& Elsevier]

Published
2009
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5. Extracellular matrix proteins protect human HT1080 cells against the antimigratory effect of doxorubicin

Author

Jean-Marie Zahm, Noël Bonnet, Roselyne Garnotel, Jean-Marc Millot, Nicolas Fourre, Emilie Millerot-Serrurot, Pierre Jeannesson, Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Plasticité de l'épithélium respiratoire dans les conditions normales et pathologiques - UMR-S 903 (PERPMP), SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre Hospitalier Universitaire de Reims (CHU Reims)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Reims Champagne-Ardenne (URCA), Zahm, Jean-Marie, Université de Reims Champagne-Ardenne (URCA)-Centre Hospitalier Universitaire de Reims (CHU Reims)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)

Subjects
Cancer Research, RHOA, Stress fiber, MESH: Cell Line, Tumor, Cell Culture Techniques, MESH: Collagen Type I, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Collagen Type I, Focal adhesion, Extracellular matrix, 03 medical and health sciences, MESH: Doxorubicin, 0302 clinical medicine, Cell Movement, Cell Line, Tumor, MESH: Fibronectins, Humans, MESH: Antibiotics, Antineoplastic, MESH: Cell Movement, 030304 developmental biology, 0303 health sciences, MESH: Cell Culture Techniques, MESH: Humans, Antibiotics, Antineoplastic, biology, Cell growth, Cell migration, General Medicine, Vinculin, Molecular biology, Cell biology, Fibronectins, Fibronectin, Oncology, Doxorubicin, 030220 oncology & carcinogenesis, biology.protein, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
Abstract

International audience; In solid tumors, the cell microenvironment appears to be a key determinant in the emergence of drug resistance, a major obstacle to the successful use of antitumor drugs. Our aim was to determine whether type I collagen and fibronectin, proteins of the extracellular matrix, were able to influence the antimigratory properties induced by the antitumor drug doxorubicin. These properties were investigated at doxorubicin concentrations of 10 and 20 nM, which do not affect cell proliferation on a 24 h drug exposure. Using videomicroscopy, we found that these subtoxic doses of doxorubicin were sufficient to inhibit individual tumor cell motion on two-dimensional plastic surfaces. Such a drug treatment induced a dramatic disturbance of actin stress fiber formation and of vinculin distribution in 80% of cells. In contrast, on extracellular matrix proteins, cell speed was unaffected by drug and perturbation of both actin network and vinculin distribution was detected in only 50% of cells, suggesting a protective effect of the microenvironment. In addition, the phosphorylation of focal adhesion kinase and GTPase RhoA was less affected by doxorubicin with cells cultured on extracellular matrix proteins. In conclusion, our findings indicate that the cell microenvironment prevents drug-dependent inhibition of cell migration in vitro. They reveal cell locomotion as a key factor of microenvironment-mediated drug resistance. This new concept needs to be exploited in in vitro models to optimize the screening of new antimigratory drugs.

Published
2008
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6. Epigallocatechin-3-gallate (EGCG) inhibits the migratory behavior of tumor bronchial epithelial cells

Author

Christine Terryn, Béatrice Nawrocki-Raby, S. Hazgui, Arnaud Bonnomet, Myriam Polette, Magali Milliot, Jérôme Cutrona, Jean-Marie Zahm, Philippe Birembaut, Plasticité de l'épithélium respiratoire dans les conditions normales et pathologiques - UMR-S 903 (PERPMP), SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre Hospitalier Universitaire de Reims (CHU Reims)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Reims Champagne-Ardenne (URCA), Biomolécules : interactions moléculaires, cellulaires et cellules-matrice extracellulaire (BIMCCME), Université de Reims Champagne-Ardenne (URCA)-Centre Hospitalier Universitaire de Reims (CHU Reims)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'histologie, cytologie, biologie cellulaire et moléculaire Pol Bouin, Université de Reims Champagne-Ardenne (URCA)-Centre Hospitalier Universitaire de Reims (CHU Reims), InCa, SPLF, ARC, Zahm, Jean-Marie, Université de Reims Champagne-Ardenne (URCA)-Centre Hospitalier Universitaire de Reims (CHU Reims)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)

Subjects
MESH: Cell Death, Time Factors, Matrix metalloproteinase inhibitor, Cell, Cell Culture Techniques, Vimentin, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Catechin, MESH: Dose-Response Relationship, Drug, 3D cell culture, 0302 clinical medicine, Cell Movement, MESH: Reverse Transcriptase Polymerase Chain Reaction, heterocyclic compounds, MESH: Cell Movement, MESH: Bronchial Neoplasms, 0303 health sciences, MESH: Protease Inhibitors, Microscopy, Video, biology, Cell Death, Reverse Transcriptase Polymerase Chain Reaction, MESH: Gene Expression Regulation, Enzymologic, Bronchial Neoplasms, food and beverages, Cell migration, MESH: Gene Expression Regulation, Neoplastic, 3. Good health, Cell biology, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, MESH: Epithelial Cells, 030220 oncology & carcinogenesis, Matrix Metalloproteinase 2, Electrophoresis, Polyacrylamide Gel, Pulmonary and Respiratory Medicine, Programmed cell death, MESH: Cell Line, Tumor, MESH: Catechin, MESH: Antineoplastic Agents, Phytogenic, MESH: Imaging, Three-Dimensional, Matrix Metalloproteinase Inhibitors, complex mixtures, Gene Expression Regulation, Enzymologic, 03 medical and health sciences, Imaging, Three-Dimensional, Cell Line, Tumor, medicine, Humans, Neoplasm Invasiveness, Protease Inhibitors, RNA, Messenger, MESH: RNA, Messenger, 030304 developmental biology, lcsh:RC705-779, MESH: Cell Culture Techniques, MESH: Humans, Dose-Response Relationship, Drug, Cell growth, Research, MESH: Time Factors, Epithelial Cells, MESH: Neoplasm Invasiveness, lcsh:Diseases of the respiratory system, Antineoplastic Agents, Phytogenic, MESH: Matrix Metalloproteinase 2, MESH: Microscopy, Video, Cell culture, biology.protein, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, MESH: Vimentin, sense organs, MESH: Electrophoresis, Polyacrylamide Gel
Abstract

Background Many studies associated the main polyphenolic constituent of green tea, (-)-Epigallocatechin-3-gallate (EGCG), with inhibition of cancers, invasion and metastasis. To date, most of the studies have focused on the effect of EGCG on cell proliferation or death. Since cell migration is an important mechanism involved in tumor invasion, the aim of the present work was to target another approach of the therapeutic effect of EGCG, by investigating its effect on the cell migratory behavior. Methods The effect of EGCG (at concentrations lower than 10 μg/ml) on the migration speed of invasive cells was assessed by using 2D and 3D models of cell culture. We also studied the effects of EGCG on proteinases expression by RT-PCR analysis. By immunocytochemistry, we analyzed alterations of vimentin organization in presence of different concentrations of EGCG. Results We observed that EGCG had an inhibitory effect of cell migration in 2D and 3D cell culture models. EGCG also inhibited MMP-2 mRNA and protein expression and altered the intermediate filaments of vimentin. Conclusion Taken together, our results demonstrate that EGCG is able to inhibit the migration of bronchial tumor cells and could therefore be an attractive candidate to treat tumor invasion and cell migration.

Published
2008
Full Text
View/download PDF

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