1. Kinin-B2 Receptor Activity in Skeletal Muscle Regeneration and Myoblast Differentiation.
- Author
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Alves JM, Martins AH, Lameu C, Glaser T, Boukli NM, Bassaneze V, Dariolli R, Nascimento IC, Martins PCM, de Souza HDN, Krieger JE, Casarini DE, Sales VM, Pesquero JB, and Ulrich H
- Subjects
- Animals, Biomarkers metabolism, Bradykinin metabolism, Cardiotoxins administration & dosage, Cell Line, Cell Proliferation, Cytoskeleton metabolism, Gene Deletion, Kininogens genetics, Kininogens metabolism, Mice, Inbred C57BL, Muscle Fibers, Skeletal cytology, Muscle Fibers, Skeletal metabolism, Myosin Heavy Chains metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Receptor, Bradykinin B2 genetics, Cell Differentiation, Muscle, Skeletal physiology, Myoblasts cytology, Receptor, Bradykinin B2 metabolism, Regeneration
- Abstract
The bioactive peptide bradykinin obtained from cleavage of precursor kininogens activates the kinin-B2 receptor functioning in induction of inflammation and vasodilatation. In addition, bradykinin participates in kidney and cardiovascular development and neuronal and muscle differentiation. Here we show that kinin-B2 receptors are expressed throughout differentiation of murine C2C12 myoblasts into myotubes. An autocrine loop between receptor activation and bradykinin secretion is suggested, since bradykinin secretion is significantly reduced in the presence of the kinin-B2 receptor antagonist HOE-140 during differentiation. Expression of skeletal muscle markers and regenerative capacity were decreased after pharmacological inhibition or genetic ablation of the B2 receptor, while its antagonism increased the number of myoblasts in culture. In summary, the present work reveals to date no functions described for the B2 receptor in muscle regeneration due to the control of proliferation and differentiation of muscle precursor cells.
- Published
- 2019
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