1. Gata4, Tbx5 and Baf60c induce differentiation of adipose tissue-derived mesenchymal stem cells into beating cardiomyocytes.
- Author
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Li Q, Guo ZK, Chang YQ, Yu X, Li CX, and Li H
- Subjects
- Adipose Tissue cytology, Cells, Cultured, Chromosomal Proteins, Non-Histone, GATA4 Transcription Factor metabolism, Gene Expression, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Humans, Mesenchymal Stem Cells cytology, Microscopy, Fluorescence, Myocytes, Cardiac cytology, Reverse Transcriptase Polymerase Chain Reaction, T-Box Domain Proteins metabolism, Transcription Factors metabolism, Transfection, Cell Differentiation genetics, GATA4 Transcription Factor genetics, Mesenchymal Stem Cells metabolism, Myocytes, Cardiac metabolism, T-Box Domain Proteins genetics, Transcription Factors genetics
- Abstract
The adipose tissue-derived mesenchymal stem cells (ADMSCs) are extensively utilized in tissue engineering, regenerative medicine and cell therapy. ADMSCs can differentiate into cardiomyocytes, and it has been shown that over-expression of a cocktail of factors can induce ectopic heart formation and program cardiogenesis in ESCs. However, which genes are responsible for differentiation of ADMSCs into beating cardiomyocyte-like cells remains unknown. In this study we have shown that the combination of Gata4, Tbx5 and Baf60c is sufficient for inducing ADMSCs to form cardiomyocytes. It also appears that, while Gata4 and Baf60c are key inducers of myocardial differentiation, Tbx5 is essential for the ability of cardiac cells to contract. These findings provide additional experimental references for myocardial tissue engineering in the emerging field of cell-based therapy of heart diseases., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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