1. Generation of mouse ES cell lines engineered for the forced induction of transcription factors
- Author
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Yong Qian, Marshall Thomas, Minoru S.H. Ko, Lina S. Correa-Cerro, Dawood B. Dudekula, Hien G. Hoang, Gregory Mowrer, Dan L. Longo, Emily A. Meyers, Yulan Piao, David Schlessinger, Jean S. Cadet, Akira Nishiyama, Bernard Y. K. Binder, Hong Yu, Alexei A. Sharov, Li Xin, Lioudmila V. Sharova, and Uwem C. Bassey
- Subjects
Genetics ,Multidisciplinary ,Microarray analysis techniques ,Gene Expression Profiling ,Cellular differentiation ,fungi ,Cell Differentiation ,Biology ,Embryonic stem cell ,Phenotype ,Genome ,Article ,Recombinant Proteins ,Cell Line ,Cell biology ,Gene expression profiling ,Mice ,Gene expression ,Animals ,natural sciences ,Genetic Engineering ,Cell Engineering ,Transcription factor ,Embryonic Stem Cells ,Transcription Factors - Abstract
Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7-10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource.
- Published
- 2011
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