1. Altered growth response of oral mucosal keratinocytes in p53-deficient mice.
- Author
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Ito, Daisuke, Kamijo, Ryutaro, Nakanishi, Vuko, Toyoshima, Takahiko, Takizawa, Kunlo, Sumitani, Kaname, Nagumo, Masao, Ito, D, Kamijo, R, Nakanishi, Y, Toyoshima, T, Takizawa, K, Sumitani, K, and Nagumo, M
- Subjects
P53 protein ,APOPTOSIS ,CELL death ,KERATINOCYTES ,CELL proliferation ,MESSENGER RNA - Abstract
P53 has important regulatory functions in cell growth, differentiation and apoptosis. Here we analyzed the effects of p53 on the growth response of oral mucosal keratinocytes (OMKCs) using p53-deficient (p53-/-) mice. No morphological difference was found between p53-/- and wild-type (p53+/+) oral mucosa. In a long-term culture, p53-/- OMKCs continued to proliferate past the point at which p53+/+ became senescent. The percentage of p53-/- OMKCs in the G0/G1 phase was lower than that of p53+/+ OMKCs. Proliferation of cultured OMKCs induced by epidermal growth factor (EGF) and interleukin-(IL)-1alpha was more strongly enhanced in p53-/- than in p53+/+ mice. Such an enhanced response was not due to increased mRNA expression of growth factor receptors. These data suggest that p53 acts as a modulator of G1 arrest in OMKCs and is also involved in the regulation of responses to EGF and IL-1alpha without affecting the expression of their receptors. [ABSTRACT FROM AUTHOR]
- Published
- 1999
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