1. A pepper (Capsicum annuum L.) metacaspase 9 (Camc9) plays a role in pathogen-induced cell death in plants.
- Author
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Kim SM, Bae C, Oh SK, and Choi D
- Subjects
- Capsicum cytology, Capsicum microbiology, Cell Death genetics, Gene Expression Regulation, Plant, Gene Silencing physiology, Genes, Plant genetics, Genes, Plant physiology, Plant Proteins genetics, Plants, Genetically Modified cytology, Plants, Genetically Modified enzymology, Plants, Genetically Modified genetics, Plants, Genetically Modified microbiology, Xanthomonas campestris pathogenicity, Capsicum enzymology, Capsicum genetics, Cell Death physiology, Plant Proteins metabolism
- Abstract
Metacaspases, which belong to the cysteine-type C14 protease family, are most structurally similar to mammalian caspases than any other caspase-like protease in plants. Atmc9 (Arabidopsis thaliana metacaspase 9) has a unique domain structure, and distinct biochemical characteristics, such as Ca²⁺ binding, pH, redox status, S-nitrosylation and specific protease inhibitors. However, the biological roles of Atmc9 in plant-pathogen interactions remain largely unknown. In this study, a metacaspase gene present as a single copy in the pepper genome, and sharing 54% amino acid sequence identity with Atmc9, was isolated and named Capsicum annuum metacaspase 9 (Camc9). Camc9 encodes a 318-amino-acid polypeptide with an estimated molecular weight of 34.6 kDa, and shares approximately 40% amino acid sequence identity with known type II metacaspases in plants. Quantitative reverse transcription-polymerase chain reaction analyses revealed that the expression of Camc9 was induced by infections of Xanthomonas campestris pv. vesicatoria race 1 and race 3 and treatment with methyl jasmonate. Suppression of Camc9 expression using virus-induced gene silencing enhanced disease resistance and suppressed cell death symptom development following infection with virulent bacterial pathogens. By contrast, overexpression of Camc9 by transient or stable transformation enhanced disease susceptibility and pathogen-induced cell death by regulation of reactive oxygen species production and defence-related gene expression. These results suggest that Camc9 is a possible member of the metacaspase gene family and plays a role as a positive regulator of pathogen-induced cell death in the plant kingdom., (© 2013 BSPP AND JOHN WILEY & SONS LTD.)
- Published
- 2013
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