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Your search keyword '"Nobuyuki Maruo"' showing total 2 results
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2 results on '"Nobuyuki Maruo"'

1. Effects of recombinant H2 relaxin on the expression of matrix metalloproteinases and tissue inhibitor metalloproteinase in cultured early placental extravillous trophoblasts

Author

Senn Wakahashi, Koji Nakabayashi, Nobuyuki Maruo, Takeshi Maruo, and Ai Yata

Subjects
endocrine system, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Down-Regulation, Matrix metalloproteinase, Biology, Cell Line, law.invention, Endocrinology, law, Internal medicine, medicine, Humans, RNA, Messenger, Receptor, Relaxin, Messenger RNA, Metalloproteinase, Tissue Inhibitor of Metalloproteinase-1, urogenital system, RNA, Matrix Metalloproteinases, Recombinant Proteins, Trophoblasts, Up-Regulation, body regions, Matrix Metalloproteinase 9, Cell culture, Recombinant DNA, Matrix Metalloproteinase 2, Matrix Metalloproteinase 3, hormones, hormone substitutes, and hormone antagonists
Abstract

Relaxin promotes softening of the uterine cervix and inhibits uterine contractility in rats, mice and pigs. Little information, however, is available about the role of relaxin in humans. In 2002, LGR7 and LGR8 were discovered to be receptors for relaxin and those receptors were identified in the human placenta. Thus, in this study, effects of recombinant H2 (rH2) relaxin on human early placental extravillous trophoblasts (EVTs) were examined. Isolation of EVTs from early placental trophoblasts was performed using the procedures established in our laboratory. After 48-h subculture, the presence of relaxin receptors in cultured EVTs was characterized by RT-PCR and immunoblotting. The cultured EVTs were treated with different doses (0.3-3 ng/ml) of rH2 relaxin for 24 h. The effects of rH2 relaxin on MMP-2, -3, -9 and TIMP-1 mRNAs levels were examined by real-time RT-PCR. RT-PCR and immunoblotting revealed that relaxin receptors are present in early placental EVTs. Treatment with rH2 relaxin increased MMP-2 and -9 mRNAs levels and decreased TIMP-1 mRNA levels in cultured EVTs, whereas rH2 relaxin did not affect MMP-3 mRNA levels. These results suggest that relaxin may promote the invasive potential of early placental EVTs through up-regulating MMP-2, -9 mRNAs and down-regulating TIMP-1 mRNA in EVTs.

Published
2007
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2. Suppression of progesterone production by stresscopin/urocortin 3 in cultured human granulosa-lutein cells.

Author

Ai Yata, Koji Nakabayashi, Senn Wakahashi, Nobuyuki Maruo, Noriyuki Ohara, and Takeshi Maruo

Subjects
PROGESTERONE, CELL culture, HORMONE receptors, FEMALE reproductive organs, ENZYME-linked immunosorbent assay, REGULATION of ovulation, RADIOIMMUNOASSAY, GENE expression
Abstract

BACKGROUND Corticotropin-releasing hormone (CRH) and its receptors have been identified in female reproductive tissues. CRH regulates follicular maturation, ovulation, luteolysis and steroidgenesis. A CRH-related peptide stresscopin (SCP), or urocortin III (Ucn3), has recently been identified, but its functions in the ovary remain to be elucidated. In the present study, we investigated the effects of SCP/Ucn3 on progesterone production in cultured human granulosa-lutein cells. METHODS The presence of SCP/Ucn3 and CRH type-2 receptor (CRHR2) in cultured granulosa-lutein cells from 21 infertile women (aged 22–36 years) was examined by RT–PCR and immunocytochemistry. The concentration of SCP/Ucn3 in follicular fluid, human serum and culture medium was examined by radioimmunoassay. Progesterone production by cultured granulosa-lutein cells treated with SCP/Ucn3 was examined by enzyme-linked immunosorbent assay. RESULTS SCP/Ucn3 and CRHR2 mRNAs and proteins were expressed in granulosa-lutein cells. SCP/Ucn3 was detected in culture media of granulosa-lutein cells and follicular fluid. Treatment of cultured granulosa-lutein cells with 0.1, 1.0 or 10 nM SCP/Ucn3 decreased progesterone secretion when compared with untreated control (all P CONCLUSIONS The present results suggest that the SCP/CRHR2 system is present in human ovaries and treatment with SCP/Ucn3 inhibits progesterone production by cultured granulosa-lutein cells through interaction with CRHR2. [ABSTRACT FROM AUTHOR]

Published
2009
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