12 results on '"Héctor Flores-Herrera"'
Search Results
2. Differential proMMP-2 and proMMP-9 secretion in human pre-implantation embryos at day 5 of development
- Author
-
Jair Lozano-Cuenca, Jorge Skiold López-Canales, Héctor Flores-Herrera, Esther Iyune-Cojab, Paola Vázquez-Cárdenas, Mauricio Osorio-Caballero, Mercedes Olvera-Valencia, and Ricardo Josué Acuña-González
- Subjects
Enzyme Precursors ,business.industry ,Embryonic Development ,Embryo ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Cell biology ,Culture Media ,Text mining ,Matrix Metalloproteinase 9 ,Gelatinases ,Pregnancy ,Humans ,Matrix Metalloproteinase 2 ,Secretion ,Female ,business - Abstract
Background: The most commonly used non-invasive criterion for evaluating the probable success of transferring in vitro human embryos for implantation is their morphological development. With this criterion, however, embryos in cellular arrests go unnoticed. Extracellular matrix metalloproteases type 2 (MMP-2) and MMP-9 are key markers of embryonic development and the implantation process, according to various animal studies. The current study investigated the proMMP-2 and proMMP-9 expression in the culture media developing human embryos that were transferred for implantation. Methods: Forty-two patients were accepted in the Department of Reproductive Biology of a Hospital in México City, based on the Institutional inclusion criteria for in vitro fertilization. On day 5 of development, embryos were transferred to women, and the culture medium was stored at -70 to await assessment of the activity of proMMP-2 and proMMP-9 in substrate gel zymography. Results: The patients showing embryo sac development were assigned to the pregnant group (n =17) or non-pregnant (n =25). In both groups, the activity of proMMP-2 and proMMP-9 was evaluated in substrate gel zymography. Our results indicate for all 17 women able to achieve a full-term pregnancy, the activity band of proMMP-2 was found in the corresponding culture medium. For 11 of them, the band of proMMP-9. Regarding the other 25 patients, the expression band for proMMP-2 detected in 3 and that proMMP-9 in 11 individuals. Conclusions: On day 5 of embryo development, the evaluation of proMMP-2 and proMMP-9 in the embryo culture medium is a reliable indicator of embryo quality and capacity to establish pregnancy.
- Published
- 2022
3. Increased systemic and peritoneal oxidative stress biomarkers in endometriosis are not related to retrograde menstruation
- Author
-
Alberto Martín Guzmán-Grenfell, Rafael Medina-Navarro, Araceli Montoya-Estrada, Oliver P. Cruz-Orozco, Héctor Flores-Herrera, Cynthia F. Coria-García, Yessica Dorin Torres-Ramos, J. J. Hicks, and Patricia Aguayo-González
- Subjects
Adult ,endometriosis ,medicine.medical_specialty ,Adolescent ,Physiology ,Clinical Biochemistry ,retrograde menstruation ,Endometriosis ,Serum Albumin, Human ,oxidative damage ,medicine.disease_cause ,Biochemistry ,Gastroenterology ,chemistry.chemical_compound ,Peritoneal cavity ,Young Adult ,Internal medicine ,Blood plasma ,medicine ,lcsh:Pathology ,Ascitic Fluid ,Humans ,lcsh:QH301-705.5 ,business.industry ,Peritoneal fluid ,Biochemistry (medical) ,Albumin ,Cell Biology ,medicine.disease ,Malondialdehyde ,Oxidative Stress ,medicine.anatomical_structure ,peritoneal fluid ,chemistry ,lcsh:Biology (General) ,Female ,Hemoglobin ,business ,Oxidative stress ,Biomarkers ,Research Article ,lcsh:RB1-214 - Abstract
Objetives: The goal of this study was to determine if systemic and peritoneal oxidative stress biomarkers are related to each other and to retrograde menstruation in endometriosis. Methods: Plasma and peritoneal fluid oxidative stress biomarkers and hemoglobin and erythrocytes in peritoneal fluid as retrograde menstruation indicators, were measured in 28 patients with endometriosis and 23 without endometriosis. Results: In the peritoneal fluid, carbonyls and lipohydroperoxides, indicative of protein and lipid oxidative damage, were higher in endometriosis group (21%, p = 0.016 and 46%, p = 0.009, respectively). However, these biomarkers were not different in the blood plasma of both groups, and only protein dityrosine, was increased in the plasma of endometriosis group (31%, p = 0.04). The peritoneal fluid hemoglobin content was not higher in the endometriosis group, nor related to carbonyls and lipohydroperoxides. Additionally, the peritoneal fluid oxidative biomarkers were not correlated with the blood plasma ones, and only malondialdehyde, and ischemia-modified albumin were almost two times higher in peritoneal fluid. Discussion: Our results show a peritoneal and systemic oxidative stress biomarkers increase in endometriosis, but not related to each other, and do not support the hypothesis of an increase in hemoglobin-iron supply towards the peritoneal cavity that causes oxidative damage.
- Published
- 2019
4. Possible mechanisms involved in the effect of the subchronic administration of rosuvastatin on endothelial function in rats with metabolic syndrome
- Author
-
Jair Lozano-Cuenca, O.A. López-Canales, Ignacio Valencia-Hernández, Jorge Skiold López-Canales, R.M. López-Mayorga, Héctor Flores-Herrera, and E.F. Castillo-Henkel
- Subjects
0301 basic medicine ,Male ,Medicine (General) ,Charybdotoxin ,K+ channel ,Physiology ,Vasodilator Agents ,Stimulation ,Pharmacology ,Biochemistry ,Glibenclamide ,chemistry.chemical_compound ,0302 clinical medicine ,General Pharmacology, Toxicology and Pharmaceutics ,Rosuvastatin Calcium ,Biology (General) ,Aorta ,General Neuroscience ,General Medicine ,Metabolic syndrome ,Vasodilation ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,medicine.drug ,Research Article ,Endothelium ,QH301-705.5 ,Immunology ,Biophysics ,Rat aorta ,Ocean Engineering ,Apamin ,NO ,Rosuvastatin ,03 medical and health sciences ,R5-920 ,Diabetes mellitus ,medicine ,Animals ,Rats, Wistar ,business.industry ,Vasorelaxation ,nutritional and metabolic diseases ,Cell Biology ,medicine.disease ,Acetylcholine ,Rats ,Disease Models, Animal ,030104 developmental biology ,chemistry ,Endothelium, Vascular ,business - Abstract
Metabolic syndrome is a multifaceted condition associated with a greater risk of various disorders (e.g., diabetes and heart disease). In a rat model of metabolic syndrome, an acute in vitro application of rosuvastatin causes relaxation of aortic rings. Since the outcome of a subchronic rosuvastatin treatment is unknown, the present study explored its effect on acetylcholine-induced vasorelaxation of aortic rings from rats with metabolic syndrome. Animals were submitted to a 16-week treatment, including a standard diet, a cafeteria-style diet (CAF-diet), or a CAF-diet with daily rosuvastatin treatment (10 mg/kg). After confirming the development of metabolic syndrome in rats, aortic segments were extracted from these animals (those treated with rosuvastatin and untreated) and the acetylcholine-induced relaxant effect on the corresponding rings was evaluated. Concentration-response curves were constructed for this effect in the presence/absence of L-NAME, ODQ, KT 5823, 4-aminopyridine (4-AP), tetraethylammonium (TEA), apamin plus charybdotoxin, glibenclamide, indomethacin, clotrimazole, and cycloheximide pretreatment. Compared to rings from control rats, acetylcholine-induced vasorelaxation decreased in rings from animals with metabolic syndrome, and was maintained at a normal level in animals with metabolic syndrome plus rosuvastatin treatment. The effect of rosuvastatin was inhibited by L-NAME, ODQ, KT 5823, TEA, apamin plus charybdotoxin, but unaffected by 4-AP, glibenclamide, indomethacin, clotrimazole, or cycloheximide. In conclusion, the subchronic administration of rosuvastatin to rats with metabolic syndrome improved the acetylcholine-induced relaxant response, involving stimulation of the NO/cGMP/PKG/Ca2+-activated K+ channel pathway.
- Published
- 2020
5. Secretion of heat shock -60, -70 kD protein, IL-1β and TNFα levels in serum of a term normal pregnancy and patients with pre-eclampsia development
- Author
-
Oscar Flores-Herrera, Néstor F. Díaz, Anayansi Molina-Hernández, Asyadette Barrera‐García, Edgar Barrientos‐Galeana, Héctor Flores-Herrera, Mauricio Osorio-Caballero, María C. Álvarez‐Cabrera, Jesus F. Acevedo, and Guadalupe García-López
- Subjects
Adult ,0301 basic medicine ,inflammatory cytokine ,medicine.medical_specialty ,Amniotic fluid ,Short Communication ,Pregnancy Trimester, Third ,Interleukin-1beta ,Short Communications ,Gene Expression ,Preeclampsia ,Young Adult ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Pre-Eclampsia ,Pregnancy ,Internal medicine ,Heat shock protein ,Lactate dehydrogenase ,Extracellular ,medicine ,Humans ,HSP70 Heat-Shock Proteins ,Aspartate Aminotransferases ,030219 obstetrics & reproductive medicine ,L-Lactate Dehydrogenase ,Tumor Necrosis Factor-alpha ,business.industry ,Alanine Transaminase ,Chaperonin 60 ,Cell Biology ,Amniotic Fluid ,medicine.disease ,pre‐eclampsia ,Uric Acid ,heat‐shock proteins ,030104 developmental biology ,Endocrinology ,chemistry ,Shock (circulatory) ,Molecular Medicine ,Uric acid ,Female ,Tumor necrosis factor alpha ,medicine.symptom ,business ,Biomarkers - Abstract
The extracellular heat shock proteins (eHsp) family act as molecular chaperones regulating folding, transporting protein and are associated with immune modulation in different physiological and pathological processes. They have been localized in different gestational tissues and their concentration in amniotic fluid and serum has been determined. In the present study, we proposed to determine the concentration of eHsp‐60, ‐70, IL‐1β and TNFα in the serum of pregnant patients with 34 weeks of gestation with and without clinical evidences of preeclampsia (PE). Our results indicate significant increase of these markers in patients with PE with respect to healthy pregnant patients without active labor. Finally, the concentration of eHsp‐60 and ‐70 correlated positively with the hepatic dysfunction markers uric acid, lactate dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT) glutamic pyruvic transaminase (GPT), and inflammatory IL‐1β and TNFα response. In conclusion, our results demonstrate a strong associated between Hsp and marker of hepatic dysfunction.
- Published
- 2018
6. In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
- Author
-
Héctor Flores-Herrera, Néstor E. Díaz-Martínez, Wendy Portillo, Guadalupe García-López, Néstor F. Díaz, Anayansi Molina-Hernández, and Daniela Ávila-González
- Subjects
0301 basic medicine ,Placenta ,General Chemical Engineering ,Biology ,Regenerative medicine ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,Antigens, CD ,Pregnancy ,medicine ,Humans ,Amnion ,Cells, Cultured ,030219 obstetrics & reproductive medicine ,General Immunology and Microbiology ,General Neuroscience ,Epithelial Cells ,Cadherins ,Epithelium ,In vitro ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Amniotic epithelial cells ,Cellular component ,Female ,Developmental biology ,Biomarkers - Abstract
Several protocols have been reported in the literature for the isolation and culture of human amniotic epithelial cells (HAEC). However, these assume that the amniotic epithelium is a homogeneous layer. The human amnion can be divided into three anatomical regions: reflected, placental, and umbilical. Each region has different physiological roles, such as in pathological conditions. Here, we describe a protocol to dissect human amnion tissue in three sections and maintain it in vitro. In culture, cells derived from the reflected amnion displayed a cuboidal morphology, while cells from both placental and umbilical regions were squamous. Nonetheless, all the cells obtained have an epithelial phenotype, demonstrated by the immunodetection of E-cadherin. Thus, because the placental and reflected regions in situ differ in cellular components and molecular functions, it may be necessary for in vitro studies to consider these differences, because they could have physiological implications for the use of HAEC in biomedical research and the promising application of these cells in regenerative medicine.
- Published
- 2019
7. Capturing the ephemeral human pluripotent state
- Author
-
Irma Lydia García-Castro, Daniela Ávila-González, Wendy Portillo, Guadalupe García-López, Héctor Flores-Herrera, Néstor F. Díaz, and Anayansi Molina-Hernández
- Subjects
0301 basic medicine ,Genetics ,Octamer Transcription Factor-3 ,Somatic cell ,Cell potential ,Cellular differentiation ,Biology ,Embryonic stem cell ,Cell biology ,Developmental dynamics ,03 medical and health sciences ,030104 developmental biology ,Induced pluripotent stem cell ,Reprogramming ,Developmental Biology - Abstract
During human development, pluripotency is present only in early stages of development. This ephemeral cell potential can be captured in vitro by obtaining pluripotent stem cells (PSC) with self-renewal properties, the human embryonic stem cells (hESC). However, diverse studies suggest the existence of a plethora of human PSC (hPSC) that can be derived from both embryonic and somatic sources, depending on defined culture conditions, their spatial origin, and the genetic engineering used for reprogramming. This review will focus on hPSC, covering the conventional primed hESC, naive-like hPSC that resemble the ground-state of development, region-selective PSC, and human induced PSC (hiPSC). We will analyze differences and similarities in their differentiation potential as well as in the molecular circuitry of pluripotency. Finally, we describe the need for human feeder cells to derive and maintain hPSC, because they could emulate the interaction of in vivo pluripotent cells with extraembryonic structures that support development. Developmental Dynamics 245:762-773, 2016. © 2016 Wiley Periodicals, Inc.
- Published
- 2016
8. Establishment of human embryonic stem cell line Amicqui-2 using poor-quality embryos from Mexican population
- Author
-
Héctor Flores-Herrera, Omar Martínez-Alarcón, Néstor F. Díaz, Juan Carlos Regalado-Hernández, Anayansi Molina-Hernández, Eva Vega-Hernández, Julio Francisco De la Jara-Díaz, Guadalupe García-López, Guadalupe Razo-Aguilera, Néstor E. Díaz-Martínez, María Yolotzin Valdespino-Vázquez, Wendy Portillo, Elsa Romelia Moreno-Verduzco, and Daniela Ávila-González
- Subjects
0301 basic medicine ,Population ,Human Embryonic Stem Cells ,Cell Culture Techniques ,Biology ,Cell Line ,Andrology ,03 medical and health sciences ,Mice ,0302 clinical medicine ,medicine ,Animals ,Humans ,education ,lcsh:QH301-705.5 ,Mexico ,education.field_of_study ,Karyotype ,Embryo ,Cell Biology ,General Medicine ,medicine.disease ,Embryo, Mammalian ,Embryonic stem cell ,Mexican population ,030104 developmental biology ,lcsh:Biology (General) ,Cell culture ,embryonic structures ,Female ,Teratoma ,030217 neurology & neurosurgery ,Developmental Biology ,Human embryonic stem cell line - Abstract
Although investigation with human embryonic stem cells (HESC) is not decreasing, the derivation of new lines has been diminished. The preeminence of only a few HESC lines in research is accompanied by lack of universal applicability of results as well as by genetic under-representation. We previously reported the derivation of one line with male karyotype from Mexican population. Here, we derived one HESC line (Amicqui-2) with female karyotype from poor-quality embryos. These line comply the pluripotent requirements (normal karyotype, detection of pluripotency-associated markers, mycoplasma test and teratoma formation) and could be a valuable model for studying diseases specific to under-represented population.
- Published
- 2018
9. Pluripotency markers in tissue and cultivated cells in vitro of different regions of human amniotic epithelium
- Author
-
Irma Lydia García-Castro, Héctor Flores-Herrera, Horacio Merchant-Larios, Néstor F. Díaz, Anayansi Molina-Hernández, Guadalupe García-López, Alejandro Sanchez-Flores, Daniela Ávila-González, Jerome Verleyen, Néstor E. Díaz-Martínez, and Wendy Portillo
- Subjects
0301 basic medicine ,Homeobox protein NANOG ,Pluripotent Stem Cells ,Placenta ,Biology ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,medicine ,Humans ,Amnion ,reproductive and urinary physiology ,Cells, Cultured ,SOXB1 Transcription Factors ,Gene Expression Regulation, Developmental ,Cell Differentiation ,Cell Biology ,Nanog Homeobox Protein ,Epithelium ,In vitro ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Epiblast ,030220 oncology & carcinogenesis ,embryonic structures ,Female ,Stem cell ,tissues ,Octamer Transcription Factor-3 ,Immunostaining ,Biomarkers - Abstract
Studies have described the presence of pluripotent markers in vivo and in vitro in human amnion. However, the amnion can be divided into reflected, placental and umbilical regions that are anatomically and functionally heterogeneous. Here, we evaluated the expression of pluripotency markers in tissue and cultivated cells in vitro of different regions of human amnion. To this end, we determined the presence of the core pluripotency factors OCT-4, NANOG and SOX-2 by immunofluorescence and RT-PCR and also performed transcriptome analysis of the different regions of amnion tissue. We identified the mRNA and protein of the pluripotency factors in the different regions of human amnion tissue. However, the OCT-4 and NANOG immunolocalization was cytoplasmic, whereas SOX-2 immunolocalization was nuclear regardless of the region analyzed. Moreover, we found three subpopulations of cells in the in vitro cultures of reflected and placental amnion: cells with immunostaining only in the nucleus, only in the cytoplasm, or in both compartments. Yet no statistically significant differences were found between the reflected and placental amnion. These results suggest a homogeneous distribution of the pluripotency transcription factors of the different regions of human amnion to isolate stem cells that can be used in regenerative medicine.
- Published
- 2018
10. Markers of Pluripotency in Human Amniotic Epithelial Cells and Their Differentiation to Progenitor of Cortical Neurons
- Author
-
Héctor Flores-Herrera, Eva Ramón-Gallegos, Guadalupe García-López, Irma Lydia García-Castro, Wendy Portillo, Néstor F. Díaz, Anayansi Molina-Hernández, and Daniela Ávila-González
- Subjects
Homeobox protein NANOG ,Pluripotent Stem Cells ,Rex1 ,Cellular differentiation ,lcsh:Medicine ,Biology ,Epigenesis, Genetic ,Kruppel-Like Factor 4 ,SOX2 ,Humans ,Amnion ,Progenitor cell ,Induced pluripotent stem cell ,lcsh:Science ,Cells, Cultured ,Cell Proliferation ,Neurons ,Multidisciplinary ,lcsh:R ,Cell Differentiation ,Epithelial Cells ,Molecular biology ,Cell biology ,Amniotic epithelial cells ,embryonic structures ,cardiovascular system ,Intercellular Signaling Peptides and Proteins ,lcsh:Q ,Stem cell ,biological phenomena, cell phenomena, and immunity ,Biomarkers ,Research Article ,Transcription Factors - Abstract
Human pluripotent stem cells (hPSC) have promise for regenerative medicine due to their auto-renovation and differentiation capacities. Nevertheless, there are several ethical and methodological issues about these cells that have not been resolved. Human amniotic epithelial cells (hAEC) have been proposed as source of pluripotent stem cells. Several groups have studied hAEC but have reported inconsistencies about their pluripotency properties. The aim of the present study was the in vitro characterization of hAEC collected from a Mexican population in order to identify transcription factors involved in the pluripotency circuitry and to determine their epigenetic state. Finally, we evaluated if these cells differentiate to cortical progenitors. We analyzed qualitatively and quantitatively the expression of the transcription factors of pluripotency (OCT4, SOX2, NANOG, KLF4 and REX1) by RT-PCR and RT-qPCR in hAEC. Also, we determined the presence of OCT4, SOX2, NANOG, SSEA3, SSEA4, TRA-1-60, E-cadherin, KLF4, TFE3 as well as the proliferation and epigenetic state by immunocytochemistry of the cells. Finally, hAEC were differentiated towards cortical progenitors using a protocol of two stages. Here we show that hAEC, obtained from a Mexican population and cultured in vitro (P0-P3), maintained the expression of several markers strongly involved in pluripotency maintenance (OCT4, SOX2, NANOG, TFE3, KLF4, SSEA3, SSEA4, TRA-1-60 and E-cadherin). Finally, when hAEC were treated with growth factors and small molecules, they expressed markers characteristic of cortical progenitors (TBR2, OTX2, NeuN and β-III-tubulin). Our results demonstrated that hAEC express naive pluripotent markers (KLF4, REX1 and TFE3) as well as the cortical neuron phenotype after differentiation. This highlights the need for further investigation of hAEC as a possible source of hPSC.
- Published
- 2015
11. Evidence of in vitro differential secretion of 72 and 92 kDa type IV collagenases after selective exposure to lipopolysaccharide in human fetal membranes
- Author
-
Veronica Zaga-Clavellina, Felipe Vadillo-Ortega, Rolando Maida-Claros, Yonathan Garfias-Becerra, Guadalupe García-López, Héctor Flores-Herrera, Horacio Merchant-Larios, Jorge Beltrán-Montoya, Mauricio Osorio, and Diana Soriano-Becerril
- Subjects
Lipopolysaccharides ,Embryology ,Extraembryonic Membranes ,Stimulation ,Enzyme-Linked Immunosorbent Assay ,Matrix metalloproteinase ,Biology ,Andrology ,Extracellular matrix ,Fetal membrane ,Genetics ,medicine ,Decidua ,Humans ,Zymography ,Secretion ,Amnion ,Molecular Biology ,Tissue Inhibitor of Metalloproteinase-2 ,Tissue Inhibitor of Metalloproteinase-1 ,Obstetrics and Gynecology ,Tissue Inhibitor of Metalloproteinases ,Cell Biology ,Extracellular Matrix ,medicine.anatomical_structure ,Reproductive Medicine ,Biochemistry ,Matrix Metalloproteinase 9 ,Collagenase ,Matrix Metalloproteinase 2 ,Female ,Developmental Biology ,medicine.drug - Abstract
Premature rupture of chorioamniotic membranes complicated with intrauterine infection has been associated to degradation of extracellular matrix (ECM), which could explain local morphological changes. We used a culture system in which the chorioamniotic membranes form two independent chambers, allowing for the selective stimulation of either the amnion (AMN) and/or the choriodecidua (CHD) regions. Lipopolysaccharide (500 ng/ml) was added to the AMN and/or the CHD; secretions and gelatinolytic activity of matrix metalloproteinase (MMP)-2 and MMP-9 were measured in both compartments by enzyme-linked immunosorbent assay (ELISA) and zymography. Secretions of TIMP-1, TIMP-2 and TIMP-4 were measured by ELISA. Both metalloproteinases were immunolocalized in tissue sections. All stimulation modalities induced a similar proMMP-2 and proMMP-9 secretion pattern in the CHD with concentrations of 2.49 ng/ml and 90.91 pg/ml, respectively; the AMN showed no significant changes. The active forms of both enzymes did not change with any stimulation modality. TIMP-1, TIMP-2 and TIMP-4 secretions remained without significant changes (P = 0.41). ECM degradation and structural disarrangement were evident after stimulation. Secretion of proMMP-2 and proMMP-9 mainly in the CHD, presence of active forms associated to the tissue and minor changes in TIMPs secretion could favor ECM degradation and explain the weakening and thinning associated with the pathological rupture of chorioamniotic membranes.
- Published
- 2007
12. Human amniotic epithelial cells as feeder layer to derive and maintain human embryonic stem cells from poor-quality embryos
- Author
-
Héctor Flores-Herrera, Elsa Romelia Moreno-Verduzco, Eva Vega-Hernández, Guadalupe García-López, Guadalupe Razo-Aguilera, Daniela Ávila-González, Néstor F. Díaz, Néstor E. Díaz-Martínez, Wendy Portillo, Anayansi Molina-Hernández, Julio Francisco De la Jara-Díaz, Irma Lydia García-Castro, and Juan Carlos Regalado-Hernández
- Subjects
KOSR ,Cellular differentiation ,Human Embryonic Stem Cells ,Biology ,Embryo Culture Techniques ,Feeder Layer ,Humans ,Amnion ,lcsh:QH301-705.5 ,Cells, Cultured ,Genetics ,Medicine(all) ,Feeder Cells ,Cell Differentiation ,Epithelial Cells ,Amniotic stem cells ,General Medicine ,Cell Biology ,Embryo, Mammalian ,Embryonic stem cell ,Cell biology ,lcsh:Biology (General) ,Karyotyping ,Amniotic epithelial cells ,embryonic structures ,Stem cell ,Transcription Factors ,Adult stem cell ,Developmental Biology - Abstract
Data from the literature suggest that human embryonic stem cell (hESC) lines used in research do not genetically represent all human populations. The derivation of hESC through conventional methods involve the destruction of viable human embryos, as well the use of mouse embryonic fibroblasts as a feeder layer, which has several drawbacks. We obtained the hESC line (Amicqui-1) from poor-quality (PQ) embryos derived and maintained on human amniotic epithelial cells (hAEC). This line displays a battery of markers of pluripotency and we demonstrated the capacity of these cells to produce derivates of the three germ layers.
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.