1. lncRNA GAS5 suppresses rheumatoid arthritis by inhibiting miR-361-5p and increasing PDK4
- Author
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Lixin Feng, Zhenshan Zhang, Nannan Xia, Feilong Meng, Lei Yang, Pengfei Xu, Bing Li, Lijuan Zhu, Luyue Zhang, Weifeng Zhang, and Zhijuan Ren
- Subjects
Chemistry ,Biophysics ,PDK4 ,Cell Biology ,Hyperplasia ,medicine.disease ,Biochemistry ,Chondrocyte ,medicine.anatomical_structure ,Lncrna gas5 ,Apoptosis ,Rheumatoid arthritis ,medicine ,Cancer research ,GAS5 ,Molecular Biology ,BAX Protein - Abstract
Rheumatoid arthritis (RA) is an inflammatory disease that causes hyperplasia of synovial tissue and cartilage destruction. This research was to investigate the effects of lncRNA GAS5/miR-361-5p/PDK4 on rheumatoid arthritis. By qRT-PCR, GAS5 and PDK4 were found to be overexpressed in synovial tissue, fibroblast-like synoviocytes of RA patients and LPS-induced chondrocytes, while the miR-361-5p expression was significantly reduced. GAS5 overexpression resulted in a decrease in the proliferation and Bcl-2 protein expression, and an increase in the Bax protein level. On the contrary, miR-361-5p sponged by GAS5 could accelerate chondrocyte proliferation, inhibit apoptosis. PDK4 targeted by miR-361-5p could inhibit RA, and partially eliminated the effect of miR-361-5p on RA. Our study suggested that GAS5 suppressed RA by competitively adsorbing miR-361-5p to modulate PDK4 expression.
- Published
- 2021