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Your search keyword '"ZILOCCHI C"' showing total 2 results
Start Over Author "ZILOCCHI C" Topic cd8-positive t-lymphocytes
2 results on '"ZILOCCHI C"'

1. IL-4-transduced tumor cell vaccine induces immunoregulatory type 2 CD8 T lymphocytes that cure lung metastases upon adoptive transfer.

Author

Rodolfo M, Zilocchi C, Accornero P, Cappetti B, Arioli I, and Colombo MP

Subjects
Animals, CD8-Positive T-Lymphocytes metabolism, CD8-Positive T-Lymphocytes transplantation, Cancer Vaccines genetics, Carrier Proteins genetics, Carrier Proteins immunology, Cell Communication immunology, Cytokines metabolism, Female, Folate Receptors, GPI-Anchored, Gene Transfer Techniques, Granulocytes immunology, Interleukin-4 genetics, Killer Cells, Natural immunology, Longevity, Lung Neoplasms immunology, Lymphocyte Activation genetics, Mice, Mice, Inbred BALB C, Mice, Knockout, Retroviridae genetics, T-Lymphocyte Subsets metabolism, T-Lymphocyte Subsets transplantation, Adoptive Transfer methods, CD8-Positive T-Lymphocytes immunology, Cancer Vaccines immunology, Interleukin-4 immunology, Lung Neoplasms secondary, Lung Neoplasms therapy, Lymphocyte Activation immunology, Receptors, Cell Surface, T-Lymphocyte Subsets immunology
Abstract

Vaccinations with tumor cells engineered to produce IL-4 prolonged survival and cured 30% of mice bearing pulmonary metastases, an effect abrogated by in vivo depletion of T cells. Vaccination induced type 2 T cell polarization in both CD4 and CD8 T lymphocyte subsets. We focused on the antitumor activity exerted by type 2 CD8+ T cells (Tc2) activated by IL-4 tumor cell vaccination. Tc2 lymphocytes lacked in vitro tumor cytotoxicity, but released IL-4 upon stimulation with tumor cells, as shown by limiting dilution analysis of the frequencies of tumor-specific pCTL and of CD8 cells producing the cytokine. In vivo fresh purified CD8+ T lymphocytes from IL-4-vaccinated mice eliminated 80-100% of lung metastases when transferred into tumor-bearing mice. CD8+ lymphocytes from IL-4-vaccinated IFN-gamma knockout (KO), but not from IL-4 KO, mice cured lung metastases, thus indicating that IL-4 produced by Tc2 cells was instrumental for tumor rejection. The antitumor effect of adoptively transferred Tc2 lymphocytes needed host CD8 T cells and AsGM1 leukocyte populations, and partially granulocytes. These data indicate that Tc2 CD8+ T cells exert immunoregulatory functions and induce tumor rejection through the cooperation of bystander lymphoid effector cells. Tumor eradication is thus not restricted to a type 1 response, but can also be mediated by a type 2 biased T cell response.

Published
1999

2. IL-4-transduced tumor cell vaccine induces immunoregulatory type 2 CD8 T lymphocytes that cure lung metastases upon adoptive transfer

Author

Rodolfo, M., Zilocchi, C., Accornero, P., Barbara Cappetti, Arioli, I., and Colombo, M. P.

Subjects
Mice, Knockout, Mice, Inbred BALB C, Lung Neoplasms, Folate Receptors, GPI-Anchored, Longevity, Immunology, Gene Transfer Techniques, Receptors, Cell Surface, Cell Communication, CD8-Positive T-Lymphocytes, Lymphocyte Activation, Adoptive Transfer, Cancer Vaccines, Killer Cells, Natural, Mice, Retroviridae, T-Lymphocyte Subsets, Animals, Cytokines, Immunology and Allergy, Female, Interleukin-4, Carrier Proteins, Granulocytes
Abstract

Vaccinations with tumor cells engineered to produce IL-4 prolonged survival and cured 30% of mice bearing pulmonary metastases, an effect abrogated by in vivo depletion of T cells. Vaccination induced type 2 T cell polarization in both CD4 and CD8 T lymphocyte subsets. We focused on the antitumor activity exerted by type 2 CD8+ T cells (Tc2) activated by IL-4 tumor cell vaccination. Tc2 lymphocytes lacked in vitro tumor cytotoxicity, but released IL-4 upon stimulation with tumor cells, as shown by limiting dilution analysis of the frequencies of tumor-specific pCTL and of CD8 cells producing the cytokine. In vivo fresh purified CD8+ T lymphocytes from IL-4-vaccinated mice eliminated 80–100% of lung metastases when transferred into tumor-bearing mice. CD8+ lymphocytes from IL-4-vaccinated IFN-γ knockout (KO), but not from IL-4 KO, mice cured lung metastases, thus indicating that IL-4 produced by Tc2 cells was instrumental for tumor rejection. The antitumor effect of adoptively transferred Tc2 lymphocytes needed host CD8 T cells and AsGM1 leukocyte populations, and partially granulocytes. These data indicate that Tc2 CD8+ T cells exert immunoregulatory functions and induce tumor rejection through the cooperation of bystander lymphoid effector cells. Tumor eradication is thus not restricted to a type 1 response, but can also be mediated by a type 2 biased T cell response.

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