1. Effects of granulocyte/macrophage colony-stimulating factor on the development and differentiation of CD5-positive macrophages and their potential derivation from a CD5-positive B-cell lineage in mice.
- Author
-
Takahashi K, Miyakawa K, Wynn AA, Nakayama K, Myint YY, Naito M, Shultz LD, Tominaga A, and Takatsu K
- Subjects
- Animals, Bone Marrow Cells physiology, Cell Differentiation drug effects, Cell Differentiation physiology, Cell Line, Cellular Senescence drug effects, Cellular Senescence physiology, Coculture Techniques, Immunophenotyping, Liver cytology, Liver embryology, Macrophages ultrastructure, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Peritoneal Cavity cytology, Stromal Cells physiology, Time Factors, B-Lymphocytes cytology, B-Lymphocytes metabolism, CD5 Antigens metabolism, Granulocyte-Macrophage Colony-Stimulating Factor pharmacology, Macrophages drug effects, Macrophages metabolism
- Abstract
In co-cultures of either the murine pre-B cell line J13, fetal liver cells, or adult peritoneal or bone marrow cells with ST2 mouse bone marrow stromal cells in the presence of granulocyte/macrophage colony-stimulating factor (GM-CSF), the development of CD5+ macrophages was demonstrated by immunohistochemical staining and flow cytometry. Although CD5+ macrophages were not present in the peritoneal cavities of normal mice, approximately 30% of the peritoneal macrophages in viable motheaten (mev/mev) mice, deficient in SHP-1 protein tyrosine phosphatase, expressed cell surface CD5 and B220, markers for B cells. In the mev/mev mice, GM-CSF level in peritoneal fluid was increased significantly. At 5 days after daily intravenous injection with GM-CSF, many CD5+ macrophages appeared in the peritoneal cavity and in omental milky spots of normal mice but fewer in osteopetrosis (op) mutant mice, deficient in macrophage (M)-CSF. These results indicate that GM-CSF, in combination with M-CSF, induces the development and differentiation of CD5+ macrophages in the peritoneal cavity, particularly in the omental milky spots of mice. In the peritoneal cavity of GM-CSF-treated mice, the percentages of hematopoietic progenitor cells doubly positive for CD5 and CD34 or c-kit and of macrophage precursor cells doubly positive for CD5 and ER-MP58 or ER-MP20 were increased significantly during the development of CD5+ macrophages and CD5 B cells, suggesting that CD5+ macrophages and B cells may share a bipotential progenitor in vivo.
- Published
- 1998