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Introduction. The functions of CD36 membrane receptor include removal of oxidized low-density lipoproteins from plasma. The aim of our study was to search association between the IVS3-6 C allele and hypercholesterolemia in overweight children. Material and Methods. The study groups comprised 55 Caucasian children with (33) and without hypercholesterolemia (22). Amplicons of exon 4 including fragments of introns 3 and 4 were studied using denaturing high-performance liquid chromatography (DHPLC). Results. Polymorphism detected by DHPLC was single nucleotide substitution in intron 3 (IVS3-6 T/C - rs3173798). The IVS3-6 T/C polymorphism is located in the region encoding the oxidized LDL binding domain, at a conserved splice site. Total serum cholesterol concentrations were significantly lower in the IVS3-6 TC heterozygotes than in the TT patients. Furthermore we found tendency (p=0.06) to lower LDL-cholesterol level in IVS3-6 TC heterozygotes than in wild-type homozygotes. Conclusion. The results of our preliminary study suggest that the IVS3-6 C allele of CD36 rs3173798 polymorphism may be associated with lower serum total and LDL-cholesterol in overweight children diagnosed with hypercholesterolemia. [ABSTRACT FROM AUTHOR]

Introduction The receptor CD36 has been reported to play an important role in atherogenicity. The aim of this study was to gain insight into the relationship between CD36 gene polymorphisms or the plasma concentration of sCD36 and clinical or biochemical parameters in children. Patients and methods The study groups comprised Caucasian children with and without hypercholesterolemia. The alterations in the CD36 gene were detected by DHPLC and the plasma concentrations of sCD36 were measured by ELISA. Results The data presented suggest that the IVS4-10A allele of CD36 (rs3211892) is associated with a lower risk of hypercholesterolemia. We observed a negative correlation of the sCD36 concentration with uric acid and insulin concentrations, the HOMA-IR ratio, weight, waist and hip circumference, systolic blood pressure, body mass index, waist–hip ratio and mean arterial pressure ratio, but a positive correlation with HDL cholesterol and ApoA1 concentrations. Female gender was a significant independent predictor of a higher plasma sCD36 concentration. Conclusions The data presented suggest a possible protective effect of a higher sCD36 concentration in relation to metabolic syndrome components. [ABSTRACT FROM AUTHOR]