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An aerobic, rod-shaped, Gram-stain-positive, actinobacterial strain, designated 1.0914 T , was isolated from a stalactite sample collected from a cave located in Guizhou Province, southwest PR China. Based on 16S rRNA gene sequence analysis, strain 1.0914 T shared highest similarities values with Nocardioides pelophilus CGMCC 4.7388 T (97.7 %), Nocardioides immobilis CCTCC AB 2017083 T (97.5 %) and Nocardioides silvaticus CCTCC AB 2018079 T (97.3 %) and values lower than 97.0 % to other members of the genus Nocardioides . Phylogenetic trees based on 16S rRNA gene sequences indicated that strain 1.0914 T formed an isolated branch with N. pelophilus CGMCC 4.7388 T , N. immobilis CCTCC AB 2017083 T and N. silvaticus CCTCC AB 2018079 T . The polar lipids contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and one unidentified phospholipid in the cellular membrane. The major fatty acids were identified as iso-C 16 : 0 , C 18 : 1  ω9 c , C 17 : 1  ω8 c and C 16 : 0 . The predominant respiratory quinone was MK-8(H 4 ) and ll-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The genomic DNA G+C content was 71.1 mol%. The orthologous average nucleotide identiy values between N. pelophilus CGMCC 4.7388 T , N. immobilis CCTCC AB 2017083 T , N. silvaticus CCTCC 2018079 T and strain 1.0914 T were 82.3, 81.7 and 81.9 % respectively. DNA-DNA hybridization values between N. pelophilus CGMCC 4.7388 T , N. immobilis CCTCC AB 2017083 T , N. silvaticus CCTCC 2018079 T and strain 1.0914 T were 25.2, 24.6 and 24.5 % respectively. The phylogenetic, phenotypic and chemotaxonomic data supported the classification of strain 1.0914 T as representing a new species of Nocardioides , for which the name Nocardioides stalactiti sp. nov. is proposed. The type strain is 1.0914 T (=CCTCC AB 2018266 T =KCTC 49243 T ).