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In the present study, the crude aqueous and chloroform extracts of Annona squamosa was tested for its in-vitro acaricidal efficacy against Rhipicephalus microplus ticks. Concentrations of 2.5%, 5%, 7.5%, 10% and 12.5% were tested against adult ticks whereas 0.312%, 0.625%, 1.25% and 2.5% were tested against larvae. No significant mortality and effect on oviposition was observed using aqueous extract, however, chloroform extract showed strong activity against both viz. adults and larvae. The highest adult mortality was observed at 12.5% concentration and determined LC50 and LC90 values were 5.24% and 14.12% respectively. A dose dependent reduction in egg laying capacity of treated female was observed in all concentrations compared to control group. The recorded mean mortality of larvae was 13.45 ± 0.73, 71.04 ± 1.10, 98.28 ± 0.39 and 100 ± 0.00 observed at 0, 0.312%, 0.625%, 1.25% and 2.5% of concentrations respectively. [ABSTRACT FROM AUTHOR]

A study was conducted to investigate the tick infestation on cattle in the plain region of Chhattisgarh state. During the period of study, 832 ticks of 47 animals were examined for the different tick species. The most prominently infested ixodid tick belonged to Hyalomma anatolicum (68.38%) followed by Rhipicephalus microplus (30.40%).The females showed higher infestation than males. A single tick species infestation pattern was observed in 17 animals while 30 animals were infested by both tick species. Single species infestation of H. anatolicum was encountered in 15 animals while R. microplus was observed in 2 animals. The intensity of tick infestation on each animal varied from 6 to 65 ticks per animal at different locations. [ABSTRACT FROM AUTHOR]