1. Genotype differentiating RT-PCR and sandwich ELISA: handy tools in epidemiological investigation of foot and mouth disease.
- Author
-
Mohapatra JK, Subramaniam S, Tosh C, Hemadri D, Sanyal A, Periyasamy TR, and Rasool TJ
- Subjects
- Animals, Antigens, Viral, Cattle, Cattle Diseases immunology, Cattle Diseases virology, Foot-and-Mouth Disease epidemiology, Foot-and-Mouth Disease immunology, Foot-and-Mouth Disease virology, Foot-and-Mouth Disease Virus classification, Foot-and-Mouth Disease Virus immunology, Genotype, India, Phylogeny, Serotyping, Cattle Diseases diagnosis, Enzyme-Linked Immunosorbent Assay, Foot-and-Mouth Disease diagnosis, Foot-and-Mouth Disease Virus isolation & purification, Reverse Transcriptase Polymerase Chain Reaction
- Abstract
Years of molecular epidemiological surveillance has revealed co-circulation of two antigenically divergent genotypes of foot and mouth disease virus serotype A in India. Genotype differentiating RT-PCR and sandwich ELISA were developed as fast, cost-effective and user-friendly alternatives to 1D region based phylogeny for detection and differentiation of genotype VI and VII. The RT-PCR assay targeting 1D region was found to be more sensitive and authentic in distinguishing genotypes than sandwich ELISA. These assays promise to be reliable tools in the epidemiological investigation of foot and mouth disease in the country.
- Published
- 2007
- Full Text
- View/download PDF