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Viral isolate FS1-43 isolated from a calf with acute respiratory tract disease was characterized as a bovine rhinovirus. This virus was antigenically indistinguishable from rhinovirus strains C-07 and VC-96. The virus replicated in low passage Madin-Darby and Georgia bovine kidney cells. A relative noncytopathogenic replication occurred in epithelial bovine turbinate, bovine fibroblastic turbinate, and bovine lung cells. Ciliary activity of tracheal explants was unaffected during eight serial passages. Viral replication was detected earlier and viral titers were higher at 33 C than at 37 C. Rotation of cultures did not affect viral titers but was necessary to produce a cytopathic effect in Madin-Darby bovine kidney cells.

Bovine adenovirus type 7 was isolated from a 10-month-old calf with fibrinopurulent pneumonia and from 2 newborn calves with pneumoenteritis. The viruses were isolated on calf lung and adrenal gland cell cultures and were identified as serotype 7 by immunoelectron microscopy and serum-neutralization tests.