Search

The contribution of colostrum to passive immunity transfer and intestinal protection is well known; however, the effects of colostrum intake on the expression of antimicrobial peptides (AP) and Fc receptors in the intestine of neonatal calves are unclear. Our aim was to investigate changes in the expression of AP and Fc receptor in the small intestine of calves in the first 36 h postpartum. Twenty-four Holstein bull calves were used in this study, of which 18 calves were administered 3.2 L of pooled colostrum for each calf per meal via an esophageal tube. Calves were slaughtered at 8 h (1 meal at 1-2 h), 24 h (2 meals at 1-2 h and 10-12 h), and 36 h (3 meals at 1-2 h, 10-12 h, and 22-24 h) postpartum. The remaining 6 calves without any milk administration were slaughtered at 2 h postpartum. Samples of blood and jejunum digesta were collected to determine immunoglobulin concentration using ELISA. Samples of the duodenum, jejunum, and ileum tissues after slaughter were collected to determine AP and Fc receptor expression using quantitative real-time PCR. In calves administered colostrum, IgG concentration in jejunum digesta rapidly decreased in an age-dependent manner (33.41, 9.47, and 0.34 mg/mL at 8, 24, and 36 h, respectively), whereas serum IgG concentration increased significantly, from 0.25 μg/mL at 2 h to 21.72 mg/mL at 24 h. Cathelicidin-4, β-defensin (DEFB)-7, and enteric β-defensin expression was upregulated at 8 h postpartum in the duodenum and jejunum compared with that at 2 h, but progressive recovery was detected from 24 h onward. Higher expression of cathelicidin-4, regenerating family member 3γ, lysozyme (LYZ), LYZ1, and LYZ2 and lower expression of DEFB, DEFB1, DEFB7, DEFB10, and enteric β-defensin were observed in the duodenum and jejunum compared with the ileum. Differences in AP expression between intestinal regions suggested that the innate immune defense mechanism varied significantly among the duodenum, jejunum, and ileum. No difference in the expression of Fc fragment of the IgG receptor was observed either among ages or small intestinal regions. The Fcγ receptor (FcγR)Ia and FcγRIIb expression was the highest at 8 h compared with that at 2, 24, and 36 h, and expression of FcγRIa, FcγRIIb, and FcγRIIIa was higher in the duodenum and jejunum than in the ileum. These results indicated that AP and Fcγ receptors might play important roles in intestinal defense during the passive immunity period., (Copyright © 2020 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

Circular RNA (circRNA) have been suggested to contribute to regulating gene expression in various tissues and cells of eukaryotes. However, little is known regarding the expression pattern of circRNA and their potential function in the small intestine of neonatal calves that receive colostrum. In the current study, jejunum tissue samples were collected from control calves (2 h after birth; CT; n = 3) and neonatal calves that ingested colostrum (24 h after birth; CO; n = 3) or milk (24 h after birth; MK; n = 3) to compare the circRNA expression patterns using a high-throughput RNA sequencing approach. A total of 21,213, 17,861, and 21,737 circRNA were identified in the CT, CO, and MK groups, respectively. Only 13,254 of these circRNA were common to the 3 groups, suggesting high specificity of circRNA expression depending on nutrient type. In total, 243, 249, and 283 circRNA were differentially expressed in the CO versus CT, CO versus MK, and MK versus CT comparisons, respectively. Gene ontology analysis showed that the differentially expressed circRNA and their predicted or known target genes from the CO and MK groups were mainly involved in macromolecule metabolic process, response to stress, and vesicle-mediated transport. Moreover, pathway analysis showed that the Rap1 signaling pathway, focal adhesion, ubiquitin-mediated proteolysis, and extracellular matrix-receptor interaction were the most significantly enriched pathways. These data collectively indicate that circRNA are abundant and dynamically expressed when calves receive colostrum and act as microRNA sponges to regulate their target genes for jejunum function during the early development of newborn calves., (Copyright © 2019 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

In ruminants, colostrum is the main source of immunoglobulins for the newborn animal, conferring immune protection until the immune system becomes active and able to synthesize its own immunoglobulins. Serotonin (5-HT), a biogenic amine derived from tryptophan, has stimulatory effects on many physiological processes, including components of the innate (mastocytes, eosinophils, and natural killer cells) and adaptive (T and B lymphocytes) immune systems. Based on the known effects of 5-HT on the immune system, we hypothesized that increased concentrations of 5-HT, through administration of its precursor 5-hydroxy-l-tryptophan (5-HTP), may positively affect development of the calf's immune system and therefore support health and growth performance during the first weeks of life. Eighteen calves were randomly assigned to 1 of 2 experimental groups (control and 5-HTP), resulting in n = 9 per treatment group. Both groups received 2 colostrum meals from a common pool of colostrum. Thereafter, calves were fed milk replacer twice daily for 30 d. In the 5-HTP group, colostrum and milk replacer were supplemented with 1.5 mg of 5-HTP/kg of birth weight during the first 15 d after birth. Body weight was recorded at birth and on d 5, 10, 15, and 30 after birth. Blood samples were collected every morning (0800 h) before feeding from birth until d 5 and then on d 7, 9, 11, 13, 15, and 30 after birth. Serum 5-HT concentrations were increased as a consequence of the 5-HTP supplementation. Plasma immunoglobulin G concentrations did not differ between groups throughout the experimental period. The blood mRNA abundance of several factors related to the innate and adaptive immune system [nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), serum amyloid A-1 (SAA1), chemokine C-C motif ligand 5 (CCL5), cyclooxygenase 2 (PTGS2), haptoglobin (HP), and IL-1β] were increased in calves supplemented with 5-HTP. Supplementation of 5-HTP did not affect any of the measured metabolites (fatty acids and glucose) or minerals (calcium and magnesium) or milk feed intake, feed conversion ratio, and growth. In conclusion, 5-HTP supplementation induced an increase of 5-HT concentrations in blood and caused an increase in mRNA abundance of several factors related to the innate and adaptive immune systems, which might increase the protection of the calf against external agents., (Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

Milk yield and quality influence calf preweaning growth and ultimately the sale value of the calf at weaning. This study was conducted to evaluate the relationships of milk production and quality of beef cows to calf preweaning ADG in beef cows sired by Bonsmara, Brangus, Charolais, Gelbvieh, Hereford, and Romosinuano and from Brangus dams to determine whether the relationships were homogeneous across cow breed group. Approximately 50 cows/yr were milked monthly for 6 mo in each of the 7 yr of this study. Milk traits were included in models as linear and quadratic covariates along with interactions of the covariates with sire breed. Tests for curvilinearity and homogeneity of regression coefficients indicated the relationship of calf preweaning ADG to milk yield and quality was quadratic and homogeneous across Charolais and Gelbvieh; linear and homogeneous across Bonsmara, Brangus, and Romosinuano; and linear and different from other sire breeds in Herefords (P < 0.05). Exceptions to this were in the regression of calf preweaning ADG on the natural logarithm of somatic cell count (SCC) and milk urea nitrogen (MUN). The relationship of calf preweaning ADG to SCC was quadratic in Brangus (P < 0.05) and linear in Gelbvieh (P < 0.05) with little evidence (P > 0.05) of a relationship in Bonsmara, Charolais, Hereford, or Romosinuano. There was little evidence (P > 0.05) of a relationship of calf preweaning ADG to MUN in any of the sire breed groups. Results from this study confirmed the importance of the influence of milk yield and quality on calf preweaning growth but indicated this influence can depend on the breed composition of the cow. Furthermore, results suggest that breed origin or adaptation may have influenced the relationships of calf preweaning ADG to cow milk yield and quality.

Colostrum (C) feeding in neonatal calves improves glucose status and stimulates intestinal absorptive capacity, leading to greater glucose absorption when compared with milk-based formula feeding. In this study, diet effects on gut growth, lactase activity, and glucose transporters were investigated in several gut segments of the small intestine. Fourteen male German Holstein calves received either C of milkings 1, 3, and 5 (d 1, 2, and 3 in milk) or respective formulas (F) twice daily from d 1 to d 3 after birth. Nutrient content, and especially lactose content, of C and respective F were the same. On d 4, calves were fed C of milking 5 or respective F and calves were slaughtered 2h after feeding. Tissue samples from duodenum and proximal, mid-, and distal jejunum were taken to measure villus size and crypt depth, mucosa and brush border membrane vesicles (BBMV) were taken to determine protein content, and mRNA expression and activity of lactase and mRNA expression of sodium-dependent glucose co-transporter-1 (SGLT1) and facilitative glucose transporter (GLUT2) were determined from mucosal tissue. Additionally, protein expression of SGLT1 in BBMV and GLUT2 in crude mucosal membranes and BBMV were determined, as well as immunochemically localized GLUT2 in the intestinal mucosa. Villus circumference, area, and height were greater, whereas crypt depth was smaller in C than in F. Lactase activity tended to be greater in C than in F. Protein expression of SGLT1 was greater in F than in C. Parameters of villus size, lactase activity, SGLT1 protein expression, as well as apical and basolateral GLUT2 localization in the enterocytes differed among gut segments. In conclusion, C feeding, when compared with F feeding, enhances glucose absorption in neonatal calves primarily by stimulating mucosal growth and increasing absorptive capacity in the small intestine, but not by stimulating abundance of intestinal glucose transporters., (Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

The objective of this study was to estimate the heritability of 3 measures of temperament in Brahman and Brahman-influenced calves (n = 1,209). Individual animal pen scores (PS) were determined by a trained observer who evaluated groups of 5 or 4 calves at a time for willingness to be approached by a human. Exit velocity (EV) was the rate (m/s) at which each calf exited a squeeze chute. Temperament score (TS) was calculated individually as (PS + EV)/2. Temperament was evaluated at 5 different times of record (28 d preweaning, weaning, 28 d postweaning, 56 d postweaning, and yearling). Contemporary groups (n = 34) comprised calves of the same sex born in the same season of the same year. There were an average of 36 calves per contemporary group and group size ranged from 3 to 78 calves. Average weaning age (186 d) ranged from 105 to 304 d. Calves were born from 2002 through 2012. Random effects included additive genetic and the permanent environmental variance. The fixed effects analyzed were age of dam, sex of calf, contemporary group, fraction of Brahman (2 levels: 1 and 0.5), age of calf at record, and weaning age. At weaning, the mean PS was 2.68 ± 0.1, the mean EV was 2.41 ± 0.1, and the mean TS was 2.48 ± 0.1. The PS was affected by fraction of Brahman (P = 0.034) and tended to be affected by age of dam (P = 0.06). The EV was affected by contemporary group (P < 0.001) and tended to be affected by weaning age (P = 0.074). Contemporary group affected TS (P < 0.001). All 3 methods of temperament evaluation were affected by time of record (P < 0.001). The regression coefficients for PS, EV, and TS were 0.0023 ± 0.0014, 0.0022 ± 0.0012, and 0.0015 ± 0.0012 m·s(-1)·d(-1) of age, respectively. Estimates of maternal genetic effects were always 0 and omitted from final models. Estimates of heritability were 0.27 ± 0.1, 0.49 ± 0.1, and 0.43 ± 0.1 for EV, PS, and TS, respectively. Estimates of permanent environmental variances as proportions of phenotypic variance were 0.33 ± 0.1, 0.23 ± 0.1, and 0.33 ± 0.1 for EV, PS, and TS, respectively. There appears to be sufficient additive genetic variance for selective improvement of temperament characteristics in Brahman cattle.

A conventional approach in dairy cow nutrition programs during late gestation is to feed moderate-energy diets. The effects of the maternal plane of nutrition on immune function and metabolism in newborn calves are largely unknown. Holstein cows (n=20) were fed a controlled-energy (CON) diet (1.24 Mcal/kg) for the entire dry period (~50 d) or the CON diet during the first 29 d of the dry period followed by a moderate-energy (OVE) diet (1.47 Mcal/kg) during the last 21 d prepartum. All calves were weighed at birth before first colostrum intake. Calves chosen for this study (n=6 per maternal diet) had blood samples harvested before colostrum feeding (d 0) and at 2 and 7 d of age. Blood samples were used to determine metabolites, acute-phase proteins, oxidative stress markers, hormones, phagocytic capacity of polymorphonuclear leukocytes (PMN) and monocytes, and total RNA was isolated from PMN. Calves from OVE dams weighed, on average, 5kg less at birth (44.0 vs. 48.6kg) than calves from CON dams. Blood glucose concentration in OVE calves had a more pronounced increase between 0 and 2 d than CON, at which point phagocytosis by PMN averaged 85% in OVE and 62% in CON. Compared with CON, calves from OVE had greater expression of TLR4, but lower expression of PPARA and PPARD at birth. Expression of PPARG and RXRA decreased between 0 and 2 d in both groups. Concentrations of leptin, cholesterol, ceruloplasmin, reactive oxygen metabolites, myeloperoxidase, retinol, tocopherol, IgG, and total protein, as well as expression of SOD2 and SELL increased markedly by 2 d in both groups; whereas, cortisol, albumin, acid-soluble protein, NEFA, insulin, as well as expression of IL6, TLR4, IL1R2, LTC4S, and ALOX5 decreased by 2 d. By 7 d of age, the concentration of haptoglobin was greater than precolostrum and was lower for OVE than CON calves. Our data provide evidence for a carry-over effect of maternal energy overfeeding during the last 3 wk before calving on some measurements of metabolism in the calf at birth and the phagocytic capacity of blood neutrophils after colostrum feeding. It might be feasible to design nutrient supplements to fortify colostrum in a way that metabolic and immunologic capabilities of the calf are improved., (Copyright © 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

Premature mortality and culling causes great wastage in the dairy industry, as a large number of heifers born never become productive or are culled before their full lactation potential is reached. The objectives of this study were to characterize survival and estimate genetic parameters for alternative longevity traits that considered (1) the survival of replacement heifers and (2) functional longevity of milking cows in the UK Holstein Friesian population, using combined information from the British Cattle Movement Service and milk recording organizations. Mortality of heifers was highest in the first month of life and was proportionately highest in calves born during winter months. Heifer mortality tended to decrease with age until about 16 mo onward; it then gradually increased, expected to be associated with culls due to reproductive failure or problems during pregnancy and calving. In milking cows, days of productive life (DPL) was analyzed as an alternative to the current trait lifespan score. Cows that died in 2009 on average lived for 6.8 yr with an average production of 4.3 yr. Heritability estimates were low for both heifer and cow survival and were ~0.01 and ~0.06, respectively. The positive genetic correlation between heifer survival with lifespan score (0.31) indicates that bulls that sire daughters with longer productive lives are also likely to have calves that survive and become replacement heifers. However, the magnitude of the genetic correlation suggests that survival in the rearing period and the milking herd are different traits. Genetic correlations were favorable between DPL with somatic cell count and fertility traits indicating that animals with a longer productive life tend to have lower somatic cell count, a shorter calving interval, fewer days to first service, and require fewer inseminations. However, an antagonistic relationship existed between DPL with milk and fat yield traits., (Copyright © 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

The aim of this study was to estimate inbreeding depression for juvenile mortality in Austrian Brown Swiss replacement heifers born in the years 2001 to 2007. After data editing, records of 69,571 animals were investigated. In total, the pedigree consisted of 203,894 animals. Mean and median inbreeding coefficients were 0.0514 and 0.0475, respectively. The following periods were defined for analyses of juvenile mortality: P1=48 h to 30 d, P2=31 to 180 d, P3=181 to the day before first calving or a maximum age of 1,200 d if no calving was reported, P4=48 h to the day before age at first calving or a maximum age of 1,200 d if no calving was reported. Mortality during the first 30 d of life was 3.2%; in the total rearing period, 9.3% of the heifers died (excluding slaughtered and exported animals). For the estimation of the effect of inbreeding and of genetic parameters a linear animal model with the random effects herd-year of birth and animal as well as the fixed effects year of birth-month of birth and dam parity, and the continuous effect of inbreeding coefficient (linear and quadratic) was applied. The model was reduced to the linear effect of inbreeding as the quadratic term was not found to have a significant effect. Inbreeding significantly affected all traits unfavorably. In the full rearing period (P4), heifer calves with an inbreeding coefficient of 0.10 had a 4.9% higher mortality rate than heifer calves with no inbreeding., (Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

The objective of this study was to evaluate the genetic relationship between postweaning weight gain (PWG), heifer pregnancy (HP), scrotal circumference (SC) at 18 months of age, stayability at 6 years of age (STAY) and finishing visual score at 18 months of age (PREC), and to determine the potential of these traits as selection criteria for the genetic improvement of growth and reproduction in Nellore cattle. The HP was defined as the observation that a heifer conceived and remained pregnant, which was assessed by rectal palpation at 60 days. The STAY was defined as whether or not a cow calved every year up to the age of 6 years, given that she was provided the opportunity to breed. The Bayesian linear-threshold analysis via the Gibbs sampler was used to estimate the variance and covariance components applying a multitrait model. Posterior mean estimates of direct heritability were 0.15 ± 0.00, 0.42 ± 0.02, 0.49 ± 0.01, 0.11 ± 0.01 and 0.19 ± 0.00 for PWG, HP, SC, STAY and PREC, respectively. The genetic correlations between traits ranged from 0.17 to 0.62. The traits studied generally have potential for use as selection criteria in genetic breeding programs. The genetic correlations between all traits show that selection for one of these traits does not imply the loss of the others.

The objectives of this study were to estimate the genetic parameters of preweaning undifferentiated bovine respiratory disease (BRD), umbilical diseases (UMB), and bloat (BLT) for a population of Holstein calves from New York State, as well as to associate the estimated breeding values determined in the current study with traits from ongoing genetic evaluations used in Canada and the United States. Data were recorded for 7,372 heifer calves at a commercial rearing facility in New York State, from arrival at 1 to 7d of age for the duration of stay at the facility. Performance and disease up to weaning and mortality before and after weaning were recorded. The 3 traits of interest, BRD, UMB, and BLT, were scored as 0 or 1 and analyzed using a multivariate linear sire model. The model included fixed effects of arrival weight, serum total protein, weaning weight, and season and year of birth; herd and sire were included as random effects. The heritabilities of the 3 health traits of interest were estimated at 0.09 for BRD, 0.14 for UMB, and 0.04 for BLT. The genetic correlation between the calf health traits BRD and BLT was 0.62. Correlations between BRD and UMB and between BLT and UMB were close to zero. Breeding values were estimated for the 3 calf health traits and correlated with routinely evaluated traits from Canadian and US genetic evaluations (correlations ranged from -0.42 to 0.32). Significant differences existed among Holstein sires for calf health during the preweaning period., (Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

Dolly, the first mammal cloned from a somatic cell, had shorter telomeres than age-matched controls and died at an early age because of disease. To investigate longevity and lifetime performance in cloned animals, we produced cloned cows with short telomeres using oviductal epithelial cells as donor cells. At 5 years of age, despite the presence of short telomeres, all cloned cows delivered multiple healthy offspring following artificial insemination with conventionally processed spermatozoa from noncloned bulls, and their milk production was comparable to that of donor cows. Moreover, this study revealed that the offspring had normal-length telomeres in their leukocytes and major organs. Thus, cloned animals have normal functional germ lines, and therefore germ line function can completely restore telomere lengths in clone gametes by telomerase activity, resulting in healthy offspring with normal-length telomeres.

The aim of this study was to estimate genetic parameters for BW of Angus cattle up to 5 yr of age and to discuss options for including mature weight (MW) in their genetic evaluation. Data were obtained from the American Angus Association. Only records from herds with at least 500 animals and with >10% of animals with BW at ≥ 2 yr of age were considered. Traits were weaning weight (WW, n = 81,525), yearling weight (YW, n = 62,721), and BW measured from 2 to 5 yr of age (MW2, n = 15,927; MW3, n = 12,404; MW4, n = 9,805; MW5, n = 7,546). Genetic parameters were estimated using an AIREML algorithm with a multiple-trait animal model. Fixed effects were contemporary group and departure of the actual age from standard age (205, 365, 730, 1,095, 1,460, and 1,825 d of age for WW, YW, MW2, MW3, MW4, and MW5, respectively). Random effects were animal direct additive genetic, maternal additive genetic, maternal permanent environment, and residual. Estimates of direct genetic variances (kg(2)) were 298 ± 71.8, 563 ± 15.1, 925 ± 52.1, 1,221 ± 65.8, 1,406 ± 80.4, and 1,402 ± 66.9; maternal genetic variances were 167 ± 4.8, 153 ± 6.1, 123 ± 9.1, 136 ± 12.25, 167 ± 18.0, and 110 ± 14.0; maternal permanent environment variances were 124 ± 2.9, 120 ± 4.3, 61 ± 7.5, 69 ± 11.9, 103 ± 15.9, and 134 ± 35.2; and residual variances were 258 ± 3.8, 608 ± 8.6, 829 ± 34.2, 1,016 ± 38.8, 1,017 ± 52.1, and 1,202 ± 63.22 for WW, YW, MW2, MW3, MW4, and MW5, respectively. The direct genetic correlation between WW and YW was 0.84 ± 0.14 and between WW and MW ranged from 0.66 ± 0.06 (WW and MW4) to 0.72 ± 0.11 (WW and MW2). Direct genetic correlations ranged from 0.77 ± 0.08 (YW and MW5) to 0.85 ± 0.07 (YW and MW2) between YW and MW, and they were ≥ 0.95 among MW2, MW3, MW4, and MW5. Maternal genetic correlations between WW and YW and MW ranged from 0.52 ± 0.05 (WW and MW4) to 0.95 ± 0.07 (WW and YW), and among MW they ranged from 0.54 ± 0.14 (MW4 and MW5) to 0.94 ± 0.07 (MW2 and MW3). Genetic correlations suggest that a genetic evaluation for MW may be MW2-based and that including BW from older ages could be accomplished by adjusting records to the scale of MW2.

The objectives of this study were to estimate the genetic parameters of survival to weaning and survival to exit for a population of Holstein calves from New York State, as well as to associate the estimated breeding values determined in the current study with traits from ongoing genetic evaluations used in Canada and the United States. Data were recorded for 7,372 heifer calves at a commercial rearing facility in New York State from arrival at 1 to 7 d of age for the duration of stay at the facility (on average, heifers departed the facility 1 mo before calving). Performance and disease up to weaning and mortality before and after weaning were recorded. Analyzed data were limited to daughters of sires with at least 10 calves originating from farms that had sent a minimum of 5 calves to be raised at the facility. As such, calves from 264 sires and 36 herds were studied using 2 statistical methods. The first method, calf survival, used a Weibull proportional hazards model, with survival defined as age at culling, death, or censoring. The second method, a 2-trait sire model, included survival from arrival to weaning (SUV1) and survival from weaning to exit (SUV2). Both models included fixed effects of arrival weight, serum total protein, weaning weight, season and year of birth, and calving ease score. Herd and sire were included as random effects. Significant associations among all fixed effects and calf survival were observed. In general, very light or heavy weight at arrival, low total protein, low weaning weight, and difficult birth increased risk of mortality for calves. The heritability of survival from the first method was 0.063. The heritabilities from the linear model were 0.001 for SUV1 and 0.036 for SUV2. The genetic correlation between SUV1 and SUV2 was 0.58. Genetic variance was close to zero for survival of calves to weaning, but was greater for survival after weaning through the growing period. Breeding values were estimated for SUV1 and SUV2 and correlated with routinely evaluated traits from Canadian and US genetic evaluations. Significant associations between genetic evaluations for survival traits and routinely evaluated traits in Canada and the United States were found, in particular with conformation body traits, somatic cell score, fertility, and longevity., (Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

Mortality in dairy cattle is not only relevant with regard to economic losses but also to animal health and welfare. Thus, the aim of this investigation was to explore the genetic background of postnatal mortality in calves and replacement heifers in different age groups until first calving in Danish Holsteins. Records of Danish Holstein heifer calves born in the years 1998 to 2007 were extracted from the Danish Cattle database (Danish Cattle, Skejby, Denmark). The following periods (P) were defined for analyses: P1=d 1 to 30, P2=d 31 to 180, P3=d 181 to 365, P4=d 366 until the day before first calving or a maximum age of 1,200 d if no calving was reported, and the full period P5=d 1 until the day before first calving or a maximum age of 1,200 d if no calving was reported. Records of animals slaughtered or exported within a defined period were set to missing for this and following periods, whereas their records were kept for preceding periods. After further data editing, more than 840,000 calves and heifers born in the years 1998 to 2007 were investigated. Mortality rates were 3.23, 2.66, 0.97, 1.92, and 9.36% for the defined periods P1 to P5, respectively. For the estimation of genetic parameters, linear and threshold sire models were applied. Effects accounted for were the random effects herd × year × season and sire as well as the fixed effects year × month, number of dam's parity (parities >5 were set to 5), calf size, and calving ease. In total, the pedigree consisted of 4,643 sires and 20,821 animals. Heritabilities for the linear model were low, ranging from 0.006 (P3) to 0.042 (P5). Heritabilities estimated by threshold models showed a wider range, from not significantly different from zero for periods with low frequencies to 0.082 for P1. The mortality rate until first calving was higher than the stillbirth rate. Genetic and phenotypic variation seemed to be sufficiently high to genetically improve the trait calf and heifer mortality. Hence, a routine genetic evaluation would be valuable for monitoring and for selecting fitter animals in the Danish Holstein cattle population., (Copyright © 2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

The objectives of this work were to estimate genetic effects for age and size at estimated time of first conception, and temperament in straightbred and crossbred heifers (n = 554) produced from Romosinuano, Brahman, and Angus cattle, and to evaluate first-parturition performance of heifers, including calf birth weight, occurrence of calving difficulty, occurrence of poor vigor in their newborn calves, and calf mortality. At approximately 7 mo of age, weaned heifers were pastured with Mashona or Tuli bulls until confirmed pregnant. Body weight, hip height, exit velocity (m/s), and chute temperament score (1 = calm, no movement; 5 = continuous movement, struggling) were recorded at 28-d intervals until heifers averaged 19 mo of age. Age at first conception was estimated as age at calving minus 285 d. Regression analyses were used to estimate BW and hip height at age of first conception. Brahman heifers were older, heavier, and had greater hip height than other straightbred groups (P < 0.05) and most crossbred groups. Brahman and reciprocal Brahman-Angus heifers had greater (P < 0.05) exit velocity than Romosinuano and Angus heifers. Brahman sire and dam breed chute temperament scores were greater (P < 0.05) than those of all other breed groups. Estimates of heterosis for age at first conception were -53.7 ± 9.5 (-11%), -56 ± 10.1 (-11%), and -92.9 ± 11 d (-18%) for Romosinuano-Brahman, Romosinuano-Angus, and Brahman-Angus, respectively (P < 0.01). Heterosis was detected (P < 0.04) for Romosinuano-Brahman for BW (12 ± 4.3 kg, 3.7%) and hip height (1.3 ± 0.6 cm, 1%) at first conception. Maternal heterosis for calf birth weight was 3.6 ± 0.5 (12%) and 2.4 ± 0.6 kg (8.6%) for Romosinuano-Angus and Brahman-Angus. In Romosinuano-Brahman and Brahman-Angus, heterosis for exit velocity was 0.23 ± 0.09 (10%) and 0.5 ± 0.1 m/s (21%). The direct breed effect of Romosinuano was to reduce age (-58.2 ± 18.9 d), BW (-57.6 ± 10.5 kg), and hip height (-2.6 ± 1.1 cm) at the time of first conception (P < 0.01), and the direct Brahman effects (P < 0.001) were large and numerically positive for these traits (169.8 ± 20.8 d, 93.3 ± 11.6 kg, and 14 ± 1.2 cm). Use of Romosinuano in crossbreeding programs with Brahman may be useful for decreasing the age at first conception. The larger birth weights of calves born to Romosinuano-Angus cross heifers would not be desirable in southern cow-calf operations.

This study investigated the effects of a SNP in the myostatin gene (MSTN or growth differentiation factor 8, GDF8) on birth, growth, carcass, and beef quality traits in Australia (Aust.) and New Zealand (NZ). The SNP is a cytosine to adenine transversion in exon 1, causing an amino acid substitution of leucine for phenylalanine(94) (F94L). The experiment used crosses between the Jersey and Limousin breeds, with the design being a backcross using first-cross bulls of Jersey x Limousin or Limousin x Jersey breeding, mated to Jersey and Limousin cows. Progeny were genotyped for the myostatin SNP and phenotyped in Aust., with finishing on feedlot (366 calves, over 3 birth years) and in NZ with finishing on pasture (416 calves, over 2 birth years). The effect of the F94L allele (A allele) on birth and growth traits was not significant. The F94L allele in Limousin backcross calves was associated with an increase in meat weight (7.3 and 5.9% of the trait mean in Aust. and NZ, respectively, P < 0.001), and a reduction in fat depth (-13.9 and -18.7% of the trait means on live calves (600 d) and carcasses, respectively, Aust. only, P < 0.001), intramuscular fat content (-8.2% of the trait mean in Aust., P < 0.05; -7.1% in NZ, not significant), total carcass fat weight (-16.5 and -8.1% of the trait mean, Aust. and NZ; P < 0.001 and P < 0.05, respectively). Meat tenderness, pH, and cooking loss of the M. longissimus dorsi were not affected by the F94L variant. In the Jersey backcross calves, additive and dominance effects were confounded because the F94L allele was not segregating in the Jersey dams. The combined effects, however, were significant on LM area (4.4% in both Aust., P < 0.05, and NZ, P < 0.01), channel fat (-11.7%, NZ only, P < 0.01), rib fat depth (-11.2%, NZ only, P < 0.05), and carcass fat weight (-7.1%, NZ only, P < 0.05). The results provide strong evidence that this myostatin F94L variant provides an intermediate and more useful phenotype than the more severe double-muscling phenotype caused by knockout mutations in the myostatin gene.

The objectives of this study were to assess the gestational, periparturient and preweaning growth traits of Holstein vs Gir x Holstein (G x H F1) cross-bred dairy calves. Holstein cows (n=64) pregnant with Holstein (H x H) calves (bulls, n=16; heifers, n=18) or G x H (bulls, n=8; heifers, n=22) calves were sampled from 60 days of gestation through calving for serum progesterone (P4) and in utero placentome sizes, and at parturition for calving ease scores (CES), calf vigour scores (CVS), and placental characteristics. Post-calving calf measures included birth weight (day 0) and body weight (kg), hip width (HW), body length (BL), wither height (WH), hip height (HH) and heart girth (HG) through 42 days of age. Serum IgG and calf vital function tests (respiration rate (RR), heart rate (HR), rectal temperature (RT) and fecal scores) were recorded on days 1, 3, 7 and 14 of age (a.m. and p.m.). Serum gestational P4 was higher (p < 0.05) for cows with H x H than G x H calves. Placentome measurements in utero were greater (p < 0.01) for H x H calves than for G x H calves, while at parturition placental characteristics did not differ (p > 0.10). Gestation length, CES and CVS also did not differ (p > 0.10) between H x H and G x H calves. Calf RR and HR were higher (p < 0.05) for H x H than G x H calves at both a.m. and p.m., while RT, fecal scores and serum IgG did not differ (p > 0.10) between H x H and G x H calves. Birth weight did not differ (p > 0.10) between H x H and G x H calves within sex. Moreover, bulls did not differ (p > 0.10) in any of the growth measures between H x H and G x H calves, whereas H x H heifers at days 28 and 42 of age were greater (p < 0.05) in all growth traits than G x H heifers. Nevertheless, the relative change in growth measures over time (days 0 to 42) did not differ (p > 0.10) between H x H and G x H calves. While subtle differences in gestational, early growth and vital function characteristics were observed, these data suggest very similar developmental profiles between H x H and G x H calves.

A total of 11,815 weight records from 23,94 Japanese Black calves was used to estimate direct, maternal, direct permanent environmental, and maternal permanent environmental effects on growth from birth to 356 d of age. The data were collected from a herd of Japanese Black cattle in Shiroshi city, Miyagi prefecture, Japan. A random regression model, including parity of dam and year-season of calving-sex of calf as fixed effects and animal, dam, animal permanent environmental, and maternal permanent environmental as random effects, was fitted to the data using Legendre polynomials for age of calf. Direct heritability estimates increased from 0.38 at birth to 0.65 at 120 d of age, decreased to 0.38 at 300 d, and then increased again up to 0.47 at 356 d. The ratio of animal permanent environmental variance to phenotypic variance decreased from 0.41 at birth to 0.12 at 90 d, and then increased gradually up to 0.40 at 270 d and oscillated around this value up to the end of the test period. Maternal genetic heritabilities increased from 0.04 at birth to 0.09 at 120 d and then decreased to 0.06 thereafter, whereas the variance ratios due to maternal permanent environment were fairly constant across the age trajectory, fluctuating around the value of 0.03. Direct genetic, phenotypic, maternal genetic, animal permanent environmental, and maternal permanent environmental correlations between different ages were all positive, and they generally decreased as the interval between ages increased. These correlations were lower between weights from nonadjacent ages than those between weights from adjacent ages. Results suggest that selection on preweaning weights would have a positive effect on weights at later ages.

The objective of this study was to investigate a potential association of an inactive myostatin allele with early calf mortality, and evaluate its effect on growth and carcass traits in a crossbred population. Animals were obtained by mating F1 cows to F1 (Belgian Blue x British Breed) or Charolais sires. Cows were obtained from mating Hereford, Angus, and MARC III (1/4 Hereford, 1/4 Angus, 1/4 Pinzgauer, and 1/4 Red Poll) dams to Hereford, Angus, Tuli, Boran, Brahman, or Belgian Blue sires. Belgian Blue was the source of the inactive myostatin allele. Myostatin genotypes were determined for all animals including those that died before weaning. Early calf mortality was examined in the F2 subpopulation (n = 154), derived from the F1 sires mated to F1 cows from Belgian Blue sires, to evaluate animals with zero, one, or two copies of inactive myostatin allele. An overall 1:2:1 ratio (homozygous active myostatin allele:heterozygous:homozygous inactive myostatin allele) was observed in the population; however, a comparison between calves dying before weaning and those alive at slaughter showed an unequal distribution across genotypes (P < 0.01). Calves with two copies of the inactive allele were more likely (P < 0.01) to die before weaning. Postweaning growth traits were evaluated in the surviving animals (n = 1,370), including birth, weaning, and live weight at slaughter, and postweaning ADG. Carcass composition traits analyzed were hot carcass weight, fat thickness, LM area, marbling score, USDA yield grade, estimated kidney, pelvic, and heart fat, retail product yield and weight, fat yield and weight, bone yield and weight, and percentage of carcasses classified as Choice. Charolais lack the inactive myostatin allele segregating in Belgian Blue; thus, in the population sired by Charolais (n = 645), only animals with zero or one copy of the inactive myostatin allele were evaluated. Animals carrying one copy were heavier at birth and at weaning, and their carcasses were leaner and more muscled. In the population sired by Belgian Blue x British Breed (n = 725), animals with two copies of inactive myostatin allele were heavier at birth, leaner, and had a higher proportion of muscle mass than animals with zero or one copies. Heterozygous animals were heaviest at weaning and had the highest live weight, whereas animals with zero copies had the highest fat content. The use of the inactive myostatin allele is an option to increase retail product yield, but considerations of conditions at calving are important to prevent mortality.

Genetic parameters for a random regression model of growth in Gelbvieh beef cattle were constructed using existing estimates. Information for variances along ages was provided by parameters used for routine Gelbvieh multiple-trait evaluation, and information on correlations among different ages was provided by random regression model estimates from literature studies involving Nellore cattle. Both sources of information were combined into multiple-trait estimates; corrected for continuity, smoothness, and general agreement with literature estimates; and extrapolated to 730 d. Covariance functions using standardized Legendre polynomials were fit for the following effects: additive genetic (direct and maternal), and animal and maternal permanent environment. Residual variances at different ages were fitted using linear splines with three knots. Fit was by least squares. The order of polynomials was varied from third to sixth. Increasing the fit beyond cubic provided small improvements in R2 and increased the number of small eigenvalues of covariance matrices, especially for the additive effect. Parameters for a random regression model in beef cattle can be constructed with negligible artifacts from literature estimates. Formulas can easily be modified for other types of polynomials and splines.

The objective of this study was to estimate genetic parameters of postnatal mortality (PM) in dairy cattle. Data originated from 841,921 Danish Holstein calves. Four binary traits of mortality were considered: D1-14, D15-60, D61-180, and D1-180 with numbers indicating the period of risk in days after birth. The unadjusted frequency of D1-14, D15-60, D61-180, and D1-180 were 0.027, 0.018, 0.020, and 0.066, respectively. A linear sire-model was fitted to the data, and average information-REML was used to estimate (co)variance components. Estimates of direct heritabilities for the four mortality traits ranged from 0.001 to 0.008 but were all significant. D61-180 and D1-180 had the highest direct heritabilities. Maternal heritabilities were very low, ranging from 0.0002 to 0.0015 and significant for D1-14 and D1-180 only. The direct genetic correlation between D1-14 and D15-60, between D15-60 and D61-180, and between D1-14 and D61-180 was 0.73, 0.54, and 0.34, respectively. It indicates that different genes are responsible for early PM (D1-14) and late PM (D61-180). When D61-180 was treated as a different trait for females, males not transferred, and transferred males, the direct heritability was 0.004, 0.008, and 0.034, respectively, but the direct genetic correlations between these three traits were very high. If transfers of calves are getting more common, the importance of including PM in a breeding program will increase, as the genetic variation of PM was considerably higher for transferred calves than for calves that were not transferred.

The purpose of this study was to compare estimates of genetic parameters for sequential growth of beef cattle using two models and two data sets. Growth curves of Nellore cattle were analyzed using body weights measured at ages 1 (birth weight) to 733 d. Two data samples were created, one with 71,867 records sampled from all herds (MISS), and the other with 74,601 records sampled from herds with no missing traits (NMISS). Records preadjusted to a fixed age were analyzed by a multiple-trait model (MTM), which included the effects of contemporary group, age of dam class, additive direct, additive maternal, and maternal permanent environment. Analyses were by REML, with five traits at a time. The random regression model (RRM) included the effects of age of animal, contemporary group, age of dam class, additive direct, additive maternal, permanent environment, and maternal permanent environment. All effects were modeled as cubic Legendre polynomials. These analyses were also by REML. Shapes of estimates of variances by MTM were mostly similar for both data sets for all except late ages, where estimates for MISS were less regular, and for birth weight with MISS. Genetic correlations among ages for the direct and maternal effects were less smooth with MISS. Genetic correlations between direct and maternal effects were more negative for NMISS, where few sires were maternal grandsires. Parameter estimates with RRM were similar to MTM cept that estimates of variances showed more artifacts for MISS; the estimates of additive direct-maternal correlations were more negative with both data sets and approached -1.0 for some ages with NMISS. When parameters of a growth model obtained by used for genetic evaluation, these parameters should be examined for consistency with parameters from MTM and prior information, and adjustments may be required to eliminate artifacts.

Purebred Angus (n = 96) and Polled Hereford (n = 96) bull calves sired by bulls of either low or high yearling weight performance lines were assigned to either creep-fed or non-creep-fed treatment before weaning for four calf crops. For each breed, after weaning in the fall, half of the calves within each of the four groups were fed a high concentrate diet for 189 d after weaning in a feedlot and the remainder were put on winter temporary pastures (rye or ryegrass) followed by summer grazing on pearl millet and bermudagrass pastures for 329 d after weaning. Dependent variables were ADG for various intervals and the complete test period, weight, height and scrotal circumference at periodic intervals, and weight per day of age at regular intervals during the test. The model included breed, performance line, sire nested within breed and line, year, age of dam, preweaning treatment, postweaning treatment, and two- and three-factor interactions. Age of calf was included as a covariate for weight, height, and scrotal circumference measurements. Line and breed differences generally were significant for weight but not for height. The interaction of primary interest (line by preweaning treatment by postweaning treatment) was not significant. The high growth performance line gained faster than low growth performance line for all intervals and were 45 kg heavier by 189 d after weaning. Preweaning treatment did not affect postweaning ADG but did affect weight per day of age. Creep-fed calves were heavier than non-creep-fed calves and maintained this advantage during the postweaning treatment periods in both the feedlot and pasture. Both preweaning treatment groups had similar ADG after weaning. Angus were 23 kg heavier at weaning than Polled Herefords; however, breed weight means were similar by 16 mo of age. In summary, bulls from different selection lines for growth were identified in feedlot and pasture when adjustments were made for prior management effects. The lack of significant interactions in these data indicates that central test station data, appropriately adjusted, are useful for ranking bulls on genetic merit for growth in feedlot and on pasture.

On 10 northern Australian properties, the number of calves sired (calf output) by individual bulls in multiple-sire matings was measured by DNA typing for paternity. There were 235 bulls (92 Santa Gertrudis, 25 5/8 Brahman and 119 Brahman) from 37 multiple-sire mating groups. Number of bulls in groups ranged from 2 to 25 and ages of bulls ranged from 2 to 5 years. Mating periods were for 3-12 months and bull mating percentages were 2.5-6%. In all, there were 4251 calves tested and the resolution of paternity ranged from 92.5 to 100% and averaged 97.7% across all sites. This included 9.9% of calves with no potential sires in any of the mating groups. Of the 235 bulls mated, 58% sired 10% or less calves in each of their respective mating groups with 6% not siring any calves. In contrast, 14% sired over 30% of the calves in each of the respective mating groups. When bulls were mated in groups of 8-24, the maximum percent of calves sired by individual bulls was 26+/-7% (mean+/-S.D.) with a range 11-36%. However, when bulls were mated in groups of 2-7, the maximum percent of calves sired by individual bulls was 59+/-19% with a range 24-94%. Calf output of bulls was moderately repeatable across years at four of five sites. Multiple regression models relating pre-mating measures of physical, seminal and behavioural traits to calf output were developed for the three breed groups. In all, only 138 of the 235 bulls were included in the models (40 Santa Gertrudis, 24 5/8 Brahman and 74 Brahman). Sheath and testicular traits, such as scrotal circumference and testicular tone, were generally not related to calf output, the exceptions being sheath depth in Brahman bulls which was negatively related (P<0.05) and scrotal circumference in 5/8 Brahmans which was positively related (P=0.08) to calf output. Dominance was only included in the 5/8 Brahman model but there was no significant relationship between dominance hierarchy and calf output. Semen motility was only related (P<0.05) to calf output in 5/8 Brahmans. However, measures of semen quality based on spermatozoa morphology were important contributors to calf output in the Santa Gertrudis and Brahman models where percent normal spermatozoa was positively related (P<0.01) to calf output. In Santa Gertrudis and Brahman bulls, measures of sexual behaviour in the serving capacity test were related to calf output. In Santa Gertrudis, these were for the number of displays of sexual interest (P<0.05), and mounts (P<0.01), but not number of serves, whilst in Brahman bulls, libido score was positively related to calf output (P<0.05). The models only explained 35-57% of the variation in calf output.

Weight records of Brazilian Nelore cattle, from birth to 630 d of age, recorded every 3 mo, were analyzed using random regression models. Independent variables were Legendre polynomials of age at recording. The model of analysis included contemporary groups as fixed effects and age of dam as a linear and quadratic covariable. Mean trends were modeled through a cubic regression on orthogonal polynomials of age. Up to four sets of random regression coefficients were fitted for animals' direct and maternal, additive genetic, and permanent environmental effects. Changes in measurement error variances with age were modeled through a variance function. Orders of polynomial fit from three to six were considered, resulting in up to 77 parameters to be estimated. Models fitting random regressions modeled the pattern of variances in the data adequately, with estimates similar to those from corresponding univariate analysis. Direct heritability estimates decreased after birth and tended to be lowest at ages at which maternal effect estimates tended to be highest. Maternal heritability estimates increased after birth to a peak around 110 to 120 d of age and decreased thereafter. Additive genetic direct correlation estimates between weights at standard ages (birth, weaning, yearling, and final weight) were moderate to high and maternal genetic and environmental correlations were consistently high.

Estimates of covariances and sire expected progeny differences of additive and nonadditive direct and maternal genetic effects for birth and weaning weights were obtained using records from 1,581 straightbred and crossbred calves from the Angus-Brahman multibreed herd at the University of Florida. Covariances were estimated by Restricted Maximum Likelihood, using a Generalized Expectation-Maximization algorithm applied to multibreed populations. Estimates of heritabilities and additive genetic correlations for straightbred and crossbred groups were within the ranges of values found in the literature for these traits. Maximum values of interactibilities (ratios of nonadditive genetic variances to phenotypic variances) and nonadditive correlations were somewhat smaller than heritabilities and additive genetic correlations. Sire additive and total direct and maternal genetic predictions for birth and weaning weight tended to increase with the fraction of Brahman alleles, whereas nonadditive direct and maternal genetic predictions were similar for sires of all Angus and Brahman fractions. These results showed that it is feasible to evaluate sires for additive and nonadditive genetic effects in a structured multibreed population. Data from purebred breeders and commercial producers will be needed to accomplish the same goal at a national level.

Heat production was measured in newborn Angus-, Brahman-, and Tuli-sired calves born to Angus (n = 20) and Brahman (n = 26) dams, before (thermoneutral metabolic rate, TMR) and after norepinephrine (NE) infusion (peak metabolic rate, PMR), to assess genotypic effects on nonshivering thermogenesis in brown adipose tissue. Calves were fed pooled colostrum, fitted with jugular catheters, and placed in a temperature-controlled (37 degrees C) water immersion system. Heat production, determined by indirect calorimetry, and tympanic temperature were measured continuously in calves from approximately 3 to 6 h of age. Blood samples were collected at birth and at 0, 5, 20, 40, 60, 80, 100, and 120 min relative to NE infusion (35 micrograms.min-1.kg BW-1 for 4 min), and plasma was analyzed for metabolites (glucose, NEFA, and urea nitrogen [PUN]) and hormones (cortisol, triiodothyronine [T3] and thyroxine [T4]). Weight-specific TMR (cal.min-1.kg-1) was not affected by breed of sire or dam, although weight-specific PMR (cal.min-1.kg-1) was lower (P < .01) in Brahman-sired calves than in Angus- or Tuli-sired calves and was lower (P < .001) in calves born to Brahman rather than Angus dams. The reduction in weight-specific PMR due to the maternal Brahman influence was sire-breed dependent, and the reduction was largest (P < .01) for Tuli-sired (34.3%), intermediate (P < .05) for Brahman-sired (15.1%), and lowest (P > .25) for Angus-sired calves (4.1%). The PMR:TMR ratio was 1.80 and 2.21 +/- .06 in calves born to Brahman and Angus dams, respectively. Peak tympanic temperature was .6 degree C lower (P < .01) in calves born to Brahman rather than Angus dams. At birth, plasma NEFA concentrations were higher (P < .001) and glucose tended (P = .13) to be higher in calves born to Brahman rather than Angus dams. Cortisol, T3, and T4 concentrations at birth were higher in calves born to Brahman dams than in those born to Angus dams. These results suggest that calves born to Brahman dams may have less thermogenically active brown adipose tissue than calves born to Angus dams, which may contribute to the relative cold intolerance of calves with Bos indicus inheritance.

An experiment involving crosses among selection and control lines was conducted to partition direct and maternal additive genetic response to 20 yr of selection for 1) weaning weight (WWL), 2) yearling weight (YWL), and 3) an index of yearling weight and muscle score (IXL). Maternal response was estimated from reciprocal crosses among unselected sires and dams of control (CTL) and the selection lines. An Angus line was added to increase the number of reciprocal cross comparisons. Direct responses of WWL, YWL, and IXL linebreds compared with CTL were significant for all traits. Maternal genetic responses were much smaller than direct responses. Direct response in birth weight was largest for YWL, followed by WWL and IXL. Maternal effect of IXL on birth weight was larger and that of WWL and YWL was smaller than CTL. Direct responses in weaning weight did not differ greatly among selection lines; maternal response was greater for IXL than for WWL, which was selected for this trait, and response was negative for YWL. Responses in maternal effects on final weight were much reduced in Hereford crosses because of a negative relation between maternal responses in pre- and postweaning gains, especially in YWL and IXL. However, in Angus crosses, a positive association between pre- and postweaning gains increased maternal responses in final weight. Direct response for postweaning gain was greater in IXL than in YWL of WWL in Hereford crosses. In Angus crosses, YWL had larger direct responses for birth weight, preweaning gain, and postweaning gain than in other lines. The direct response for muscle score from selection in IXL, which was selected for muscle score and yearling weight, was greater than in other lines; maternal response was not important. The greatest gain in final weight was obtained when selection resulted in a favorable change in the total of direct and maternal effects pre- and postweaning, which in this experiment was provided by including a muscle score along with yearling weight as selection criteria.

Growth was examined in bovine fetuses and neonates that typically differ in mature size and postnatal developmental pattern. Pregnancies were established from matings expected to produce early (E), late (L), and intermediate (I) maturing postnatal growth patterns. Tissues were collected at 100 and 200 d of gestation and 30 d postnatal. Muscle:body weight ratios were lower at 100 and 200 d for the E maturity type than for the L maturity type (P < .05). This differs from observations of muscle:body weight ratios made at 30 d postnatal, at which time ratios for E were either greater than (triceps brachii, P < .05) or similar to those for L. Few differences due to maturity type were observed at 100 d for bone weight:body weight ratios; however, at 200 d of gestation E bone weight:body weight ratios were generally lower (P < .05) than those for L. The genotypic relationship for bone weight:body weight ratio at 30 d postnatal was similar to that observed at 200 d of gestation. Observations of organ weight:body weight ratios revealed no clear patterns due to maturity type. The genotypic relationship for total muscle DNA content was similar to that observed for muscle weight. These results indicate that fetal muscle development differs in cattle that have different postnatal growth patterns by as early as 100 d of gestation and that differences in fetal muscle growth are related to differences in muscle hyperplasia.

Body measurements on Angus- (n = 374) and Polled Hereford-sired (n = 438) calves were used to quantify calf shape via multivariate factor analysis, and measurements on Polled Hereford-sired calves were further used to investigate relationships between calf shape and sire expected progeny differences (EPD) for birth weight and first-calf calving ease. Calf birth weight, head circumference, shoulder width, hip width, heart girth, cannon bone circumference and length, and body length were recorded at 24 h postpartum. Sire birth weight EPD was positively associated (P less than .05) with all calf measures but only with head and cannon circumference after adjustment for phenotypic birth weight and gestation length. Thus, at constant birth weight, calves from higher birth weight EPD bulls tended to have larger cannon and head circumferences. Calving ease EPD was negatively related (P less than .05) to all calf measures except shoulder width. After adjustment for birth weight, only cannon circumference was associated with calving ease EPD, and this effect was removed by additional adjustment for birth weight EPD. Thus, at constant birth weight, bulls with higher EPD for calving ease sired calves with smaller cannon circumference, but at constant birth weight EPD, body measures were not associated with calving ease EPD. Factor analysis defined underlying skeletal width and frame components of shape (independent of birth weight) for both sire breeds. Calf shape differed among sires and was in part explained by birth weight EPD. No additional variation in shape was explained by calving ease EPD. Calf shape seemed to add no information for prediction of dystocia to that provided by birth weight EPD.

Simmental bulls (n = 27) were divergently selected on linear model first-calf calving ease expected progeny difference (CEPD) relative to birth weight expected progeny difference (BEPD) so that body measures of calves from sires whose progeny tended to be born either with more or less dystocia than expected from BEPD could be obtained. At birth, calf weight, head circumference, shoulder width, hip width, heart girth, cannon bone circumference and length, and body length were recorded for 204 calves. These measures had also been obtained from the Polled Hereford X Angus dams of the calves at their births. Sire differences (P less than .05) existed for calf cannon bone circumference and length before and after adjustment for gestation length and birth weight of the calf. Sire BEPD was positively associated with cannon and head circumferences independent of phenotypic birth weight and gestation length. No relationship existed between CEPD or threshold model first-calf calving ease expected progeny difference and any calf measure, either before or after adjustment for birth weight. Multivariate factor analysis was used to describe independent components of skeletal width, frame, and thickness after removal of birth weight effects; factors were not related to genetic merit for calving ease or observed calving performance. Independent of weight, newborn calf measures were not highly related to body measures at weaning or to dam birth measures. Body shape differences at constant weight existed in crossbred calves, but they were not related to sire genetic merit for calving ease or measured dystocia. Selection for calf body shape, independent of birth weight, would not reduce dystocia.

Serum samples were collected between 24 and 48 h of age from 408 Herefored calves of a Selection Experiment and from 200 Angus, Hereford and Red Poll calves of another experimental population (Germ Plasm Utilization Project). Concentrations of immunoglobulin G1 (IgG1) were determined by single radial immunodiffusion. Breed or selection line of calf and age of dam were the most important factors influencing IgG1 concentrations (P less than .01). In the Germ Plasm Utilization (GPU) herd, Angus calves were highest, Red Polls were intermediate and Herefords were lowest in serum concentrations. Calves from Hereford lines selected for weaning weight, yearling weight or an index based on yearling weight and muscling score were lower in IgG1 concentration than calves from the randomly selected control line. In both populations immunoglobulin levels in the calf increased as age of dam increased. In Selection Experiment Herefords, increased calving difficulty was associated with a decrease in calf IgG1 levels (P less than .05). Heritabilities of calf serum IgG1 concentration, estimated from paternal half-sib analysis on a within line or breed basis, were .03 +/- .09 and .13 +/- .19 in the two populations. In the Selection Experiment Herefords, when the component of variance for selection line was added both to the numerator and denominator of heritability, the revised estimate was .09 +/- .09. Heritabilities of maternal effects on IgG1 concentration were also estimated (by nesting calves within maternal grandsires) on a within line or breed basis. These heritabilities were .23 +/- .17 and -.07 +/- .27 for the Selection Experiment and GPU populations, respectively. In the Selection Experiment population, when the variance component for selection line was added to the numerator and denominator of maternal effects heritability, the estimate was .27 +/- . .17. Those calves that died had a lower mean IgG1 concentration than the population average (P less than .01).

The patterns of plasma concentrations of thyroxine (T4), triiodothyronine (T3) and cholesterol were determined in tropically adapted (ZX) and temperate (SH) breeds of cattle in the first 6 days of their lives in a tropical environment. T4 concentrations were high at birth and fell to within normal adult values at 6 days of age. In contrast, plasma cholesterol levels were low at birth and increased daily to the highest values on day 6. T3 levels increased from birth to maximum values at 12 h after birth and then declined progressively. The T4/T3 molar ratios were high at birth and decreased to lower values on day 2. T4 and cholesterol levels were higher (p less than 0.001) in ZX than in SH calves, and in calves born in winter were higher (p less than 0.001) in females than in males. There were significant (p less than 0.001) animals within-breed differences in all parameters. The results are discussed with reference to the use of physiological and biochemical indices for the early selection of cattle suited to tropical conditions.