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Your search keyword '"Souza, Aline Moreira de"' showing total 2 results
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2 results on '"Souza, Aline Moreira de"'

1. Bartonella henselae and Bartonella clarridgeiae infection, hematological changes and associated factors in domestic cats and dogs from an Atlantic rain forest area, Brazil.

Author

Silva, Beatriz Teixeira Gomes da, Souza, Aline Moreira de, Campos, Sabrina Destri Emmerick, Macieira, Daniel de Barros, Lemos, Elba Regina Sampaio de, Favacho, Alexsandra Rodrigues de Mendonça, and Almosny, Nádia Regina Pereira

Subjects
*FELIDAE, *CATS, *DOGS, *BARTONELLA henselae, *RAIN forests, *ANIMAL housing
Abstract

Abstract Cats are considered main reservoir of Bartonella henselae , which is transmitted to other cats especially through Ctenocephalides felis fleas, and to humans through scratching and biting. Serra da Tiririca State Park (PESET) is an Atlantic Forest area that shelters a wide variety of endemic fauna. Recently, the park has been suffering due to irregular housing construction and domestic animal population that interacts with humans and wildlife. Given that surveillance policies for animals are part of the global Strategic Framework for One Health, the aim of this study was to detect Bartonella spp. DNA in cats and dogs, evaluating laboratory changes and associated factors. Blood samples of 124 dogs and 89 cats were collected for hematology and serum chemistry analysis. DNA was extracted and tested by conventional polymerase chain reaction (PCR) targeting a fragment of the citrate synthase (glt A) gene of Bartonella spp. with specific primers. Positive samples were sequenced to identify species. Bartonella henselae and B. clarridgeiae were detected in 24.7% of cats, being, for our knowledge, the first report of B. clarridgeiae in cats from Rio de Janeiro, Brazil. None of the samples obtained from dogs tested positive in the PCR assays. No statistical significance was observed in physical and laboratory exams. We suggest that cats that inhabit PESET can be considered sources of Bartonella sp. for other cats and humans. We highlight that infected cats did not present clinical or laboratory alterations. We alert for the need of care measures, avoiding scratch and bite, particularly in immunocompromised people. [ABSTRACT FROM AUTHOR]

Published
2019
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2. Molecular and metagenomic diagnostic methods of anaplasmataceae agents in domestic felids (domiciled and errant) and opossums of Metropolitan Region of Rio de Janeiro

Author

Guimar?es, Andresa, Baldani, Cristiane Divan, Dumler, John Stephen, Carvalho, Fab?ola Marques de, Souza, Aline Moreira de, Machado, Carlos Henrique, and Balthazar, Daniel de Almeida

Subjects
gatos, diagn?stico, diagnosis, cats, hemoparasitosis, hemoparasitoses, Medicina Veterin?ria
Abstract

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2019-04-05T17:52:54Z No. of bitstreams: 1 2017 - Andresa Guimar?es.pdf: 1801107 bytes, checksum: e4ad1dcf396d5aa7844e5fbab6a2a85a (MD5) Made available in DSpace on 2019-04-05T17:52:54Z (GMT). No. of bitstreams: 1 2017 - Andresa Guimar?es.pdf: 1801107 bytes, checksum: e4ad1dcf396d5aa7844e5fbab6a2a85a (MD5) Previous issue date: 2017-07-04 CAPES Anaplasmataceae agents are gram-negative bacteria that are found in leukocytes, platelets or erythrocytes, forming morulae, characteristic of their infection. The transmission of these agents to man and animals occurs mainly by arthropod vectors. The participation of domestic felines and wild reservoirs has not yet been defined, with few studies on hemoparasitosis affecting these animals. The present study had as objective to investigate the presence of infection by Anaplasmataceae family agents in domestic felines and possums (Didelphis sp.) of the state of Rio de Janeiro, using molecular methods. We sought to sequence the PCR positive samples for validation of the same and to compare them with those deposited in Genbank, as well as to develop new diagnostic methods aiming at fast and high yield results, such as "deep sequencing", used in metagenomic assays. The aim of this study was to determine which hematological changes (hemogram, hematological morphology and hemoparasite inclusions) are more frequent and which associated factors are related to these in two distinct cat populations in the Metropolitan Region of Rio de Janeiro. Two populations of domestic cats, wandering cats (n = 105) and domiciled cats (n = 178) from the Metropolitan region of Rio de Janeiro were studied. Also, were studied 37 opossums collected from nature for rehabilitation, these were tested for the same agents as cats. The diagnosis of Ehrlichia canis, E. chaffeensis and Anaplasma spp. was performed by Polymerase Chain Reaction (PCR). In the comparison between the two populations of cats, there were significant differences between them (p

Published
2017

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